Bioquell WIC-1 User manual

©Bioquell UK Ltd (2013). All rights reserved.
USER MANUAL
TD064-O&M-001
REVISION 4
Bioquell UK Ltd
52 Royce Close
West Portway
Andover
Hampshire SP10 3TS
T:
F:
E:
W:
[phone]
[phone]
[e-mail]
www.bioquell.com
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“Walk-in” Bio-decontamination Chamber
USER MANUAL

USER MANUAL
TD064-O&M-001
REVISION 4
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Contents
1SAFETY ................................................................................................ 5
2WALK-IN CHAMBER EXPLAINED............................................................... 8
2.1 System Description ......................................................................... 8
2.2 Configurations ................................................................................ 8
2.2.1 Product Range .......................................................................... 8
2.2.2 Chamber Doors ........................................................................ 8
2.2.3 Compressed Air ........................................................................ 9
2.2.4 Air Purge Boost (APB)................................................................ 9
2.2.5 HVAC ...................................................................................... 9
2.2.6 Ceiling Mounted Aeration Unit..................................................... 9
2.2.7 Floor Standing Aeration Unit....................................................... 9
2.3 Vapour Disinfection Process............................................................ 10
2.3.1 Conditioning Stage.................................................................. 10
2.3.2 Gassing Phase ........................................................................ 10
2.3.3 Dwell Phase ........................................................................... 10
2.3.4 Aeration Phase ....................................................................... 10
3HPV GENERATOR CONNECTION ............................................................. 11
Connections to HPV Generator............................................................... 11
4OPERATING INSTRUCTIONS.................................................................. 13
4.1 Loading the Chamber .................................................................... 13
4.2 Running a Cycle............................................................................ 14
4.2.1 Running an Air Clean Up Cycle.................................................. 14
4.2.2 Running a Bio-Decontamination Cycle ....................................... 15
4.2.3 Confirmation of End of Cycle .................................................... 20
4.3 Unloading the Chamber ................................................................. 21
4.4 Running a Generator Self-Bio-decontamination Cycle ........................ 22
5SYSTEM ADMINISTRATION ................................................................... 23
5.1 WIC ............................................................................................ 23
5.2 System Engineering ...................................................................... 23
5.3 System Administration................................................................... 24
5.3.1 System Information ................................................................ 24
5.3.2 Manage Passwords .................................................................. 24
5.3.3 Screen Management................................................................ 26
5.3.4 Time and Date........................................................................ 26

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REVISION 4
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5.4 Log OFF ....................................................................................... 26
6MAINTENANCE .................................................................................... 27
6.1 Cleaning ...................................................................................... 27
6.2 Scheduled Maintenance ................................................................. 27
6.2.1 Parts to be replaced ................................................................ 28
6.2.2 Functional Checks ................................................................... 28
7TROUBLESHOOTING ............................................................................ 29
7.1 Alarms......................................................................................... 29
7.2 Fault Finding ................................................................................ 31
8PARTS LIST......................................................................................... 32
8.1 Replacement Parts ........................................................................ 32
9SPECIFICATION................................................................................... 33
10 EC DECLARATION OF CONFORMITY .................................................... 34
11 SCREEN NAVIGATION....................................................................... 35
12 WARRANTY AND TERMS OF USE ........................................................ 37
13 DEFAULT PASSWORDS ..................................................................... 37

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TD064-O&M-001
REVISION 4
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1SAFETY
Safety instructions that must be observed when operating or servicing the “Walk-
In” Bio-decontamination Chamber (WIC) are listed below.
Danger and Warning signs are used where there is a potential hazard to
personnel.
The mandatory symbol is used to describe other safety precautions that
should be followed before operating the equipment.
DANGER –CHEMICAL INJURY HAZARD
HANDLING HYDROGEN PEROXIDE
Wear personal protective equipment including eye protection, neoprene or
vinyl gloves and protective clothing.
HYDROGEN PEROXIDE LIQUID can cause burns and blistering to the
skin and tissue damage to the eyes when in contact.
HYDROGEN PEROXIDE VAPOUR can cause irritation to eyes, nose,
throat, lungs and skin and breathing difficulties and coughing.
IN ALL CASES SEEK MEDICAL ADVICE.
ACTION TO BE TAKEN ON EXPOSURE TO HYDROGEN PEROXIDE
EYES
Irrigate with sterile water for at least 10 minutes.
LUNGS
Remove casualty immediately to fresh air, rest and keep warm.
SKIN
Drench with water. If spilled on clothing remove immediately and wash
thoroughly.
MOUTH
Rinse thoroughly with water and give plenty to drink. Do not induce
vomiting.
THE WALK-IN BIO-DECONTAMINATION CHAMBER AND ASSOCIATED
HPV GENERATOR ARE ONLY TO BE USED BY PERSONNEL WHO HAVE
BEEN TRAINED BY BIOQUELL ON ITS SAFE USE

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REVISION 4
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SPILLAGES OF H2O2
Mop up with plenty of water and run to waste, diluting at least 20:1.
HANDLING HYDROGEN PEROXIDE BOTTLE
Wear protective gloves. Inspect outer bag containing peroxide bottle for
signs of liquid before opening. If any liquid is present in the bag DO
NOT OPEN. Discard safely.
If there are no signs of liquid in the bag open carefully and remove
bottle. Keep bottle upright at all times.
Do not squeeze bottle.
Do not remove the bottle cap at any time or harmful liquid may spill
out.
ENCLOSURE INTEGRITY
Ensure that the chamber’s seals are not damaged and there has been no
damage which could affect its integrity and thus lead to hydrogen
peroxide vapour escaping.
HPV GENERATOR HOSE CONNECTION
Ensure that the hoses are connected between the HPV Generator and
the WIC correctly. The rings on the end of the Camlock levers ought to
be fixed in the closed position with the hose connected.
BEFORE RUNNING A CYCLE
Ensure all personnel are out of the chamber and close the door
Check that all hoses and tubes are properly connected and secured in
place.
DURING A CYCLE
Do not disconnect the hoses
Do not attempt to open the enclosure.
AT THE END OF AERATION
Check that the vapour concentration is at, or below, the safe
occupational exposure level (OEL) before opening the enclosure. This is
to be done by an independent means using the sample point.
IF THE CYCLE ABORTS DURING GASSING OR AERATION
For the chamber to allow entry a successful Aeration is required. Run an
Aeration only cycle. Only enter once vapour concentration is at, or
below, the OEL.
VISIBLE LIQUID in the enclosure must be treated as concentrated
Hydrogen Peroxide. Observe Hydrogen Peroxide handling precautions
and remove liquid by diluting with water at least 20:1 and wiping up.

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DISPOSAL OF USED HYDROGEN PEROXIDE BOTTLES
The used Hydrogen Peroxide bottles may contain small quantities of
Hydrogen Peroxide liquid. During disposal observe all precautions for
handling Hydrogen Peroxide. Keep the bottle upright. Dispose of in
accordance with local regulations or standard operating procedures.
DANGER –BIOHAZARD
Do not disconnect hoses or attempt any repairs before the bio-
decontamination cycle is complete.
WARNING –EQUIPMENT DAMAGE
Avoid moving any component once a cycle has been started.
Keep the hoses off the floor and avoid kinks and undue strain.
Operate the equipment strictly in accordance with the Operating
Instructions and SOPs.
Repairs or adjustments to the equipment should only be made by
trained Bioquell personnel. Maintenance performed by unqualified
personnel or installation of unauthorised parts could invalidate the
warranty, cause personal injury or result in damage.
The equipment must be serviced in accordance with the Maintenance
Schedule to comply with the warranty and to maintain safe performance.
Potential hazards or failure of performance may occur if the equipment is
not serviced by trained and authorised personnel. Contact Bioquell to
schedule preventative maintenance or for emergency repairs.
DANGER –PERSONAL INJURY HAZARD
The equipment components contain electrical connections. Always
disconnect the power supply before opening access covers for inspection
or maintenance. The electrical compartments should only be opened by
a trained electrician. Failure to do so may cause an ELECTRICAL INJURY
HAZARD.

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REVISION 4
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2WALK-IN CHAMBER EXPLAINED
2.1 System Description
The “Walk-in” bio-decontamination chamber, hereinafter referred as WIC, is for
decontamination of material loads or equipment entering or exiting controlled
areas, with floor level loading/unloading.
The WIC has two principle modes of operation:
1. Air Clean Up cycle
2. Hydrogen Peroxide Vapour disinfection cycle
The “Air Clean Up cycle” provides particulate clean up to EU Grade C/B (US
Class 100,000/10,000) particle conditions (as required), via H14 grade inlet HEPA
filtration. The particulate clean up time is a validated time based on clean up from
the actual environmental conditions on the “dirty” or Classified EU Grade (US
Class) loading side to the required level, typically that of the “Clean” / production
side.
The WIC doors are interlocked so that no two doors are open at the same time
and the Clean Up is completed after loading and before the other side door can be
opened. Each time a door is closed clean up will be completed automatically
before re-entry (door opening) is possible.
The “Hydrogen Peroxide Vapour disinfection cycle” is an automated cycle
for disinfection of material outer surfaces.
The WIC requires a Hydrogen Peroxide Vapour (HPV) Generator for material
surface bio-decontamination. The HPV Generator can be connected either room
side or in a separate technical area. The Hydrogen Peroxide supply may be
integrated into the WIC when connected to an integrated HPV Generator (IG-1).
2.2 Configurations
The WIC can be configured to client specifications:
2.2.1 Product Range
The WIC can be supplied in three standard depths; 2700mm, 3100mm or
3500mm (external dimensions).
There are two widths available (internal dimensions 1680mm or 2180mm).
The internal height of the WIC is either 2200mm (standard) or 2600mm (for
chambers with an internal ceiling mounted aeration unit installed).
2.2.2 Chamber Doors
The WIC doors are supplied as standard in Phenolic Resin with double glazed
6mm thick toughened glass and integrated H2O2test port. An upgrade to
stainless steel 304 or 316 is available.

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2.2.3 Compressed Air
The facility may not have a compressed air supply available to the chamber for
operation of the door seal pneumatics. One available option is for the system to
include an onboard compressed air system.
2.2.4 Air Purge Boost (APB)
If the chamber has an optional Air Purge Boost system installed then a fan will
draw air into the chamber and a valve system will direct the air either through
the chamber or bypass directly to the extract duct.
Note: APB will always require a dedicated extract.
2.2.5 HVAC
If the facility has an HVAC system connected, the chamber will be supplied with
inlet and outlet connection points.
2.2.6 Ceiling Mounted Aeration Unit
Optional aeration functionality can be supplied by a ceiling mounted aeration unit
(Aeroflow 2000).
This internal shot of a WIC shows a
ceiling mounted aeration unit installed.
2.2.7 Floor Standing Aeration Unit
Aeration functionality can alternatively be supplied by a floor standing aeration
unit, for example the Aeroflow R2 or Bioquell R-10. Additional power sockets can
be supplied if required.
Additionally mains sockets are provided
for equipment which requires to be
powered during bio-decontamination
and for floor mounted aeration units.

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2.3 Vapour Disinfection Process
The vapour disinfection process consists of the following stages:
The HPV generator operates in a total recirculation mode when connected to the
chamber to be bio-decontaminated.
2.3.1 Conditioning Stage
During this period all the pipes and the Chamber are conditioned to a stable
temperature. This time also allows all the instruments to warm-up and stabilize.
Conditioning is a timed phase and at the end of this pre-programmed time the
cycle automatically progresses to the next phase.
2.3.2 Gassing Phase
During this phase Hydrogen Peroxide Vapour is supplied to the chamber via the
supply hose and distributed around the chamber via the 2 off stirrer fans.
As this is a closed loop process the returning air/vapour mixture from the
Chamber continues to pass through the gassing circuit for a pre-programmed
period. At the end of the pre-set time the cycle automatically progresses to the
next phase.
2.3.3 Dwell Phase
During this phase the Generator operates in the same manner as Gassing except
that Hydrogen Peroxide can be injected at a reduced rate or not at all for a
defined period. Both the injection rate and dwell period are user defined. At the
end of the pre-programmed time the cycle automatically progresses to the next
phase.
2.3.4 Aeration Phase
The Aeration phase (if the system has this functionality configured) involves the
catalytic conversion of the H2O2vapour present to a low level allowing safe entry
into the chamber. Aeration is provided either by a floor standing Aeration unit,
ceiling mounted aeration unit, buildings HVAC system or a combination of these.
CONDITIONING
GASSING
DWELL
AERATION

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REVISION 4
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3HPV GENERATOR CONNECTION
Note: The following instructions assume that the HPV Generator is
located next to the chamber with a power outlet socket nearby. In other
configurations the HPV Generator may be installed via fixed pipework in
a technical space away from the chamber, or integrated into the
chamber.
Refer to the User Manual for the Generator with regard to loading Hydrogen
Peroxide and setting up ready for operation.
Connections to HPV Generator
For HPV disinfection cycles, connections to the Generator can be via fixed
installation pipework (if generator is to be located in a technical space away from
the WIC) or by flexible hosing (as standard supplied with the Generator).
Positioning of the WIC inlet / outlet connections will vary dependent upon Client
requirements.
For both fixed pipework and flexible
hosing the connections to the
Generator and WIC are Camlock
connections.
Camlock connector.
The H2O2return hose is fitted with a male Camlock connection to attach to the
Generator and a female Camlock connection at the remote end to connect to the
chamber. (Note: The colour coding of the hose and connector is blue).
The H202supply hose is fitted with a female Camlock connection to attach to the
Generator and a male Camlock connection at the remote end to connect to the
chamber. (Note: This insulated hose goes to the red colour coded
connection).
The communication cable connection may be located in various positions on the
chamber dependent upon Client requirements.
There is a dedicated mains socket to power the HPV generator if the Generator is
placed at the front of the WIC. If it is not located at the front then a dedicated
power socket outlet must be within 2m of the HPV generator.

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Connect hoses to generator as shown in
photo.
Connect the communications cable to
the generator.
Secured Camlock arms.
Connect hoses to chamber as shown in
photo.
Connect the communications cable to
the chamber.
Secured Camlock arms.
Note: It is recommended that the
Camlock arms once secured to the
generator and chamber are secured
by cable ties.
Switch on the WIC.
Switch on Generator.
Press anywhere on the WIC screen to
access the main menu.
If power is removed, integrity may be affected.

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4OPERATING INSTRUCTIONS
4.1 Loading the Chamber
Follow the on-screen instructions to open the loading door.
Press “WIC”.
Press “UNLOCK DOOR”.
Display shows that Door A has priority,
i.e. loading from Door A.
Whilst the pneumatic seals are
releasing the display will show “DOOR
UNLOCKING”. Do not try to force the
door open.
Open door.
If required the light of the ceiling
aeration unit can be switched ON.
Dependent upon the ventilation setup, door unlocking maybe interlocked with its
operation. Hence, the time taken to unlock doors is dependent upon interlocking
setup.

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When the door is “UNLOCKED”
whichever door is opening is
highlighted yellow.
Open the door.
Any items which are required to be
operating during the cycle, such as
ventilation units and electronic
equipment (to ensure good gas
distribution within them); ought to be
plugged in to the power sockets.
Close the door.
The above screens would be displayed for a system that uses an Air Purge Boost
system for ventilation.
4.2 Running a Cycle
4.2.1 Running an Air Clean Up Cycle
Use the drop down arrow to select the
operating mode.

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During operation both Door A and Door
B status will display locked and closed
and the “UNLOCK DOOR” button is not
visible.
Count down time.
When the AIR CLEAN UP is complete
press the now visible “UNLOCK DOOR”.
Either door can be opened.
4.2.2 Running a Bio-Decontamination Cycle
Ensure the HPV Generator is connected and operational in accordance with the
instructions within the specific Generator User Manual.
When about to start a cycle check that all the connections have been made
correctly.
Check the hose carrying vapour from the Generator into the chamber is
connected, and the Camlock arm rings are secured and locked at both
ends.
Check the hose returning the vapour from the Chamber to the Generator
is connected and the Camlock arm rings are secured and locked at both
ends.
Check the communications cable plug is connected into the back of the
Generator and into the Chamber.

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Press “HPV CYCLE”if enabled;
otherwise the cycle will have to be run
from the Generator or the other door
screen.
Note: The following screens, prior to starting a cycle, will differ
dependent upon which HPV generator is connected to the WIC. When
connected to an IG-1 or L-3 the data from the RFID tag on the Hydrogen
Peroxide bottle is displayed on screen and the control system prevents
the cycle from running with invalid or insufficient Hydrogen Peroxide.
The Bioquell L-2 does not have this functionality and as such the
Operator must ensure enough Peroxide is available in the bottle.
Bioquell L-2:
Bioquell IG-1/L-3:
The cycle can be selected by pressing
on the black space and selecting the
cycle number.
To record the equipment to be
decontaminated; enter in the black
space using the keyboard which will
appear. This reference will display on
all screens and will be printed.
When setup is complete press the tick
icon. To enable the decontamination
process to commence press the yellow
confirmation button that appears when
connection setup is complete.
Press the >> button to view the
chamber in OFF mode prior to
confirming setup complete.

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Bioquell L-2:
Bioquell IG-1/L-3:
In either case:
If the cycle cannot be started then the
screen will be displayed without the
connection setup complete prompt.
Press << button to return to main
menu or >> button to view status of
chamber in OFF mode.
The screen shown on the left shows
insufficient Hydrogen Peroxide to run
the selected cycle; equally the setup
prompt may be hidden due to invalid
peroxide, bottle housing left open or
RFID tag not read.
This displays the ambient conditions
within the chamber in OFF mode.
View these conditions graphically by
pressing “WIC TREND” button.

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WIC trend screen showing ambient
conditions within chamber in OFF
mode.
To start cycle ensure generator is connected properly, press << button and
confirm connection complete.
Bioquell L-2:
Bioquell IG-1/L-3:
In either case:
To initiate the cycle press the tick icon.
Or to return to main menu press the
<< button.

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The phase of the cycle that the
chamber is in, is displayed.
This screen displays conditions within
conditioning phase of cycle.
Press “WIC TREND” to display ambient
conditions within chamber.
This screen displays the chamber
during the gassing phase.
If the cycle is aborted once in the gassing phase the system will automatically go
into Emergency Aeration. On completion the Operator has to confirm by pressing
“COMPLETE CHECK <1PPM?”.
Otherwise, the cycle continues.

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This screen displays the chamber
during the dwell phase.
This screen displays the chamber
during the aeration phase.
4.2.3 Confirmation of End of Cycle
At the end of Aeration the display will
prompt the Operator to check the
Hydrogen Peroxide concentration in the
chamber.
Press “OK?”
The concentration can be checked from either side using an independent low level
sensor through one of the test points. It is recommended that this check is done
on a regular basis. Alternatively a hand held sensor can be used to check the
levels directly within the chamber after opening the door.