Accuris MW9600 User manual

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MW9600 Microplate Washer
User Manual
Version 1.1
Accuris Instruments is a division of
Benchmark Scientific.

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Thank you for purchasing our MW9600 Microplate Washer. This user manual
describes how the instrument functions, and also includes an operation guide
and important safety information, please read it carefully before operating and
keep this manual for future reference.
Initial check
Upon receipt, please check the outer package for damage, and check all parts
inside the package to make sure all parts and components are included and in
proper condition. If anything is missing or damaged, please contact your
distributor or Benchmark Scientific immediately.
Packaging
Please keep the box and packaging material for your microplate washer. In the
event that service is required, the box will be needed for shipping the instrument
to our Service Department.

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Safety warning and guidelines
1 Important guidelines
2 Safety
In order to avoid injury, please read this manual completely before operating
the instrument. Pay special attention to all safety tips.
This product is for indoor use only.
This instrument should only be operated by trained laboratory personnel.
Do not attempt to repair the instrument without receiving specific instructions
from Bechmark Scientific’s Service Personnel. Please contact your
distributor or Benchmark Scientific to arrange service or repair.
This instrument is intended for research only.
Warning: Risk of biological contamination. All samples used for testing,
quality control or calibration should be considered potentially infectious,
and any parts of the instrument that contact the samples should also be
considered at potentially infectious. Always wear gloves and appropriate
safety gear when operating this device.
Never place a hand on or near any moving parts when the instrument is
running.

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3 Maintenance
The plate holder and platform should be cleaned regularly with alcohol to remove any
dried solutions or debris.
The Rinse function should be used to wash the tubing and wash heads. If the wash
head’s metal tubed become clogged, they can be cleared using the included cleaning tool.
This instrument should be operated in a low humidity, low dust environment,
away from direct sunlight, heat and magnetic fields.
Always make sure to use the correct input voltage. If the power cable is
damaged, replace it with same type.
Turn off the power and unplug the instrument if not being used for a long period
of time. All bottles should and tubing should be rinsed and emptied. When
storing, cover the instrument to protect it from dust.
Shut off the power immediately if any of the following occur and then contact your
distributor of Benchmark Scientific’s Service Department:
★Fluid enters into the instrument
★The instrument is exposed to rain or water
★There are any unusual noises or smells coming from the instrument
★The instrument is dropped or the outer housing is broken
★There are any obvious malfunctions

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CONTENTS
Chapter 1 Introduction...................................................................................................1
Chapter 2 Specifications...............................................................................................2
Chapter 3 Instrument Overview.................................................................................. 3
Chapter 4 Installation.....................................................................................................5
Chapter 5 Start Up and Software................................................................................ 8
Chapter 6 Preparing Wash and Waste Bottles......................................................17
Chapter 7 Creating a new wash protocol............................................................... 18
Chapter 8 Running a wash protocol........................................................................ 22
Chapter 9 Importing, Exporting and Deleting Programs....................................25
Chapter 10 Power Off...................................................................................................28
Chapter 11 Maintenance............................................................................................. 29
Chapter 12 Trouble Shooting.....................................................................................30
Appendix：MW9600 diagram.....................................................................................31

Microplate Washer User Manual Chapter 1 Introduction
─1─
Chapter 1 Introduction
The MW9600 Microplate Washer is an accessory to a Microplate Reader, and is designed
for accurate and efficient washing and preparation of ELISA plates prior to reading. The
MW9600 is compatible with standard 96-well, SBS-footprint microplates.
Features:
4.3 inch LCD display for easy programming and operating.
Reliable, high efficiency liquid pump
8 and 12-needle wash heads, suitable for micro plates with Flat, U, V, and C type well
bottoms.
Wash head positions are accurately controlled to +/- 0.1mm.
Multiple wash programs can be set up, stored and selected.
Program allows selecting specific rows for washing.
Pause function allows pausing the currently running program.
Wash bottles include clearly marked volume graduations to visually monitor liquid
levels.
Automatic monitoring and warnings when wash liquid levels are low or if waste liquid
level is high.
Efficient aspiration of liquid from plate wells, remaining liquid per well: ≤1uL.
Plate platform includes a beveled spill well. Any spilled or leaked fluid is extracted
automatically to avoid cross contamination.

Microplate Washer User Manual Chapter 2 Specifications
─2─
Chapter 2 Specifications
Operating conditions
Operating temperature range：4C45C
Operating relative humidity：≤70%
Power supply：AC 90-240V 50/60Hz
Parameters and features
Parameters Model
MW9600
Wash heads
8 channel or 12 channel
Wash modes
1 point，2 point，or 3 point aspiration
Compatible Micro
plates
96 wells, standard SBS dimensions
Compatible Well
bottoms
Flat, U, V, C bottom
Residual liquid
volume
≤1uL(each well)
Dispensing Volume
Range
50uL~2000uL
Wash cycles
1~99
Programs
100
Rinse function time
range
00:00～03:59 (mm: ss)
Soak and shaking
time range
00:00～99:00 (mm: ss)
Wash bottle level
warning
Yes, sensors included in wash and rinse bottles
Wash solution
channels
3 bottles
Waste bottle level
warning
Yes, sensor detects when bottle is full
Waste aspiration
Yes
Dimensions
(WXDXH)
345 x 460 x 200mm / 13.5 x 18 x 8.5 inches
Net weight
11.5kg / 25.3 Lbs

Microplate Washer User Manual Chapter 3 Instrument Overview
─3─
Chapter 3 Instrument Overview
Wash bottle A
Wash bottle B
Wash bottle R
Waste bottle W
Transparent Cover
Wash
LCD display
Display housing
Rear housing
Plate holder
Platform

Microplate Washer User Manual Chapter 3 Instrument Overview
─4─
Connect a flash drive to the USB port to transfer programs between
different MW9600 washers.
Wash head 2 wash heads available:1X8 channel and 1X12 channel:
1X12 channel wash head
USB port
1X8 channel wash head
USB port
SD card slot
Waste W exit
Waste W
negative pressure
Bottle R inlet
Bottle B inlet
Bottle A inlet
Bottle A fluid level warning
Bottle B fluid level warning
Bottle R fluid level warning
Bottle W fluid level warning
Power switch

Microplate Washer User Manual Chapter 4 Installation
─5─
Chapter 4 Installation
Initial Inspection
Thoroughly check the package and instrument upon receipt. Please contact your distributor or
Benchmark Scientific immediately if:
The package has been damaged or deformed during shipping.
The packaging is wet.
Any parts or accessories are missing (compare to the packing list). Make sure all ordered
items are included.
There is any damage to the Microplate Washer or accessories.
Installation steps
Open the package, remove the four bottles, wash heads, power supply and the instrument
and place in onto a sturdy, flat and level desk or lab bench.
Attention: Remove the shipping safety lock before operating.
Unscrew the allen screw on the wash head rack using the included allen
key.

Microplate Washer User Manual Chapter 4 Installation
─6─
Wash head installation
Connect the silicone tubes per the following picture:
Insert the wash head into the rack and connect the silicone tubing to instrument as pictured below.
(1X8 and 1X12 wash heads are installed in the same manner).

Microplate Washer User Manual Chapter 4 Installation
─7─
Wash bottles connections: Connect bottle tubing and level detection lines according to the
charts:
The standard included accessories are 3pcs wash bottles (2.5L) and 1pc waste bottle (2.5L) and
silicone tubing A, B, R and W.
The names of the wash bottles are Wash Solution A, Wash Solution B, Rinse Solution R and
Waste.
Power supply connection :Connect the Washer to the appropriate AC power source
Silicone Tube
from:
Instrument
Connection
Port
Color
Bottle W
non-pressure
output tube
Non-
pressure
Yellow
Bottle A fluid
output
Washing A
Black
Bottle B fluid
output
Washing B
Green
Bottle R fluid
Output
Rinse R
Purple
Bottle W fluid
input
Waste
Red
Level detection
line:
Instrument
Connection
Port
Bottle A fluid level
warning
Alert A
Bottle B fluid level
warning
Alert B
Bottle R fluid level
warning
Alert R
Bottle W fluid level
warning
Alert W
Black
Green
Red
Yellow
Purple
Black (A)
Green (B)
Purple (R)
Red (W)
Yellow (W)

Microplate Washer User Manual Chapter 5 Start Up and Software
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Chapter 5 Start Up and Software
Start Up Self-Test
When the power is first turned on, the system will perform a quick self-test.
Do not turn off the power, or insert or remove a flash drive during the
self-test.
Do not touch the wash head, wash head rack or plate holder when running.
An alert sound and warning will be displayed if the Waste Bottle W is full or
if A, B or R are empty, or any of the sensors are not properly connected.
In the event of this type of start-up warning, check the liquid levels and
sensor connections. Then push OK or F4 to cancel the warning.
Main Interface

Microplate Washer User Manual Chapter 5 Start Up and Software
─9─
F1
F2
F3
F4
Push the start/Pause button in the main menu, it will start to run
the program; push again, it will pause the program.
Browse with the direction keys.
Push and hold any key for the cursor to move faster in the
direction you choose.
F1, F2, F3, F4 are shortcut keys.
Push Rinse button in the “Wash” interface to rinse or prime the
tubing. Pressing a second time will pause the priming. Press F4 key
to cancel.
The stop button is used to stop a program during
operation.

Microplate Washer User Manual Chapter 5 Start Up and Software
─10 ─
Main menu
1. Protocols Choose the protocols icon by pressing the UP, DOWN, LEFT,
RIGHT buttons to highlight the icon, and then push OK to confirm. The
Protocol File List will open.
Protocol File List
Use the UP, DOWN, and OK buttons to highlight an existing saved protocol, and
press OK to select it. Or use the function keys F1 to F4 to select New, Delete,
Import/Export, or Back.
The bold numbers and letters on the keypad correspond to the
columns of wells on the ELISA plate when selecting the columns to
be washed. Numbers 1-12 are used for the 1x8 head, letters A to
H when using the 1x12 head).
The lower cap letters are used when naming protocols.

Microplate Washer User Manual Chapter 5 Start Up and Software
─11 ─
See Chapter 7 for Creating a wash program and Chapter 8 for Running a wash program
2. Settings: Setting options include: Purge volume setting, sensor setting,
injection index, beeper switch setting, and purge at power on.
Purge Volume setting: Choose “Purge Volume” in the menu, then press “OK”.
This allows setting of the volume of the solution which will be purged through the
tubing during the start of a protocol run. Volume can be adjusted from 5ml to
100ml by 5 ml increments. The default purge volume is 50ml.
Note: When a protocol is selected that uses a different wash bottle than used
in the previously run protocol, the purge function will run before the washing
steps. This purge function will replace the liquid in the dispensing tubes and
wash heads with the appropriate buffer for the current wash protocol. When a
wash protocol is repeated, the purge function does not run, because the tubing
and wash heads will already contain the appropriate solution.

Microplate Washer User Manual Chapter 5 Start Up and Software
─12 ─
Liquid Sensor: This allows turning on or off the sensors A, B, and R.
Note: Sensor W for Waste Liquid Level cannot be set to off.
Injection Index: This is for calibrating the set dispensing volume. The range of
the Injection Index is 0-2.00, and can be adjusted in increments ±0.01. The
default value is 1.00.
Beeper switch: The audible beep for indicating an alarm or warning can be
turned on or off.
Purge at Power On: When the first wash protocol is run after the instrument is
powered on, the purge function will run to remove bubbles and prime the
dispensing tubes and wash heads. This can be set for “Single” or “Multiple”.
“Single” setting: Only the single wash solution that is selected in the protocol
will purge through the tubes and wash head prior to washing.
“Multiple” setting: All of the active wash solutions (Solutions A, B, R, when
liquid level sensors are enabled) will purge through the dispensing tubing and
wash heads.
3. Maintenance: This menu includes the Rinse setting, 1X8 channel
calibration and 1X12 channel calibration.
Rinse: The rinse function can be used for priming the dispensing tubes (removing air
bubbles) and also for rinsing out the dispensing tubes and wash head. It is

Microplate Washer User Manual Chapter 5 Start Up and Software
─13 ─
recommended that wash solution bottle R is filled with distilled water, and this is used for
rinsing after using the microplate washer. It is recommended to use the rinse function
before powering off the instrument if it will not be used for a while.
Select which tubing line is to be rinsed, and rinse time.
Select tubing line from bottle A, B or R
Set the rinse time: 00:01-03:59 (mm:ss), the default time is 2:00.
Rinse
1X8 Calibration and 1x12 Calibration：
It’s recommended to calibrate the position of the wash head before use,
although it has been calibrated in the factory with standard micro plates.
Vertical and horizontal positioning can be adjusted in increments of 0.1mm
Choose 1x8 or 1x12 channel wash head, depending on which is used,
press OK to enter into the calibration interface.
Attention! Confirm that the proper wash head is selected
before calibration.
The rinse function can be activated by pressing the “Rinse” button.

Microplate Washer User Manual Chapter 5 Start Up and Software
─14 ─
Left deviation: Left deviation means the distance between the starting
position of the dispensing needle to the center of the left row of wells of the
micro plate.
Choose the left deviation function in the calibration menu, then push OK
button. The plate will move so that the wash head needles are aligned
above the left row of wells. Use the UP or DOWN arrow buttons to move
the needles left or right, respectively, to position the needles directly above
the center of the wells. Push OK button to confirm and save the setting.
Right deviation: Right deviation means the distance from the starting position
of the needles to the center of the right row of wells. Adjust the position using
the up and down arrows so the needles are centered above the right most row
of the microplate.

Microplate Washer User Manual Chapter 5 Start Up and Software
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Top deviation allows adjusting the distance between the wash head
bottom to the photoelectric switch.
Choose the top deviation function in the calibration menu, then push OK
button to enter. Push UP and DOWN to adjust the distance.
The distance between the needle bottom and the top of the micro plate
should be approximately 2-3mm.
Bottom deviation is the distance between the wash head bottom to the
photoelectric switch.
Choose the top deviation function in the calibration menu, then push OK
button to enter. Push UP and DOWN to adjust the distance.
The distance between the needle bottom to the bottom of the microplate
well should be about 0.5-1mm.