Agilent Technologies InfinityLab LC Series User manual

1290 Infinity II
Preparative Open-Bed Sampler/Collector
Agilent InfinityLab LC Series
User Manual

1290 Infinity II Preparative Open-Bed Sampler/Collector User Manual
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Document No: D0000567
EDITION 11/2019
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1290 Infinity II Preparative
Open-Bed Sampler/Collector
User Manual

1290 Infinity II Preparative Open-Bed Sampler/Collector User Manual 3
In This Guide
This manual contains technical reference information about the Agilent 1290
Infinity II Preparative Open-Bed Sampler/Collector (G7158B).
1Introduction
This chapter gives an introduction to the module and an instrument overview.
2 Site Requirements and Specifications
This chapter provides information on environmental requirements, physical and
performance specifications.
3 Using the Sampler/Collector
This chapter explains the essential operational parameters of the module.
4 Preparing the Sampler/Collector
This chapter explains the operational parameters of the module.
5Troubleshootingand Diagnostics
This chapter gives an overview about the troubleshooting and diagnostic features
and the different user interfaces.
6ErrorInformation
This chapter describes the meaning of error messages, and provides information
on probable causes and suggested actions how to recover from error conditions.
7 Maintenance
This chapter describes the maintenance of the module.
8 Parts for Maintenance and Repair
This chapter provides information on parts for maintenance and repair.

1290 Infinity II Preparative Open-Bed Sampler/Collector User Manual 4
9IdentifyingCables
This chapter provides information on cables used with the module.
10 Hardware Information
This chapter describes the module in more detail on hardware and electronics.
11 Appendix
This chapter provides additional information on safety, legal, and web.

1290 Infinity II Preparative Open-Bed Sampler/Collector User Manual 5
Contents
1Introduction8
Intended Use 9
Product Description 10
Features 11
Overview of the Module 12
Leak and Waste Concept 14
2 Site Requirements and Specifications 17
Site Requirements 18
Physical Specifications 23
Performance Specifications 24
3 Using the Sampler/Collector 30
Warnings and Cautions 31
Solvent Information 32
Turn on/off 43
Status Indicators 44
Drawer Status Indicator 45
Install the Preparative Loop 46
Install or Replace the Analytical Loop 50
Exchange Drawers 51
Exchange Containers 53
Replace the Fraction Collection Inlet/Outlet Tubings 55
Configuration and Operation of the Open-Bed Sampler/Collector 60
Prepare the Module for Transportation 73

1290 Infinity II Preparative Open-Bed Sampler/Collector User Manual 6
4 Preparing the Sampler/Collector 76
Best Practices 77
Capillary Color Coding Guide 79
Swage Fittings 80
Setting up the Sampler/Collector with the Instrument Control
Interface 82
Preparative Sampler Method Parameter Settings 93
Fraction Collector Method Parameter Settings 110
Pooling 118
Challenging Samples 119
Analytical to Preparative Switching Procedure 120
Recommended Vials, Well Plates, and Containers 121
5 Troubleshooting and Diagnostics 123
User Interfaces 124
Agilent Lab Advisor Software 125
6 Error Information 126
What Are Error Messages 127
General Error Messages 128
7 Maintenance 134
Introduction to Maintenance 135
Warnings and Cautions 136
Cleaning the Module 138
Overview of Maintenance 139
Install and Remove the Top Fume Hood 140
Clean the Leak Pan 142
Replace the Dripping Adapter for the Fraction Valve 143
Replace the Rotor Seal 145
Replace the Stator 149
Replace the two Peristaltic Pump Cartridges 151
Replace the Needle Wash Plug Assembly 153
Install or Replace the Sampling Unit Tubing Kit 156

1290 Infinity II Preparative Open-Bed Sampler/Collector User Manual 7
Install or Replace the Solvent Distribution Tubing 157
Install or Replace the Needle Tubing 159
Replace the Module Firmware 161
8 Parts for Maintenance and Repair 162
Supported Containers 163
List of Recommended Fraction Tubes 164
Sampler/Collector Accessory Kit 165
Tubing Kits 166
Top Fume Hood Kit 170
9 Identifying Cables 171
Cable Overview 172
Analog Cables 174
Remote Cables 176
CAN/LAN Cables 180
RS-232 Cables 181
USB 182
10 Hardware Information 183
Firmware Description 184
Electrical Connections 187
Interfaces 189
Setting the 6-bit Configuration Switch 196
Early Maintenance Feedback 200
Instrument Layout 201
11 Appendix 202
General Safety Information 203
Waste Electrical and Electronic Equipment (WEEE) Directive 209
Radio Interference 210
Sound Emission 211
Agilent Technologies on Internet 212

1290 Infinity II Preparative Open-Bed Sampler/Collector User Manual 8
1Introduction
Intended Use 9
Product Description 10
Features 11
Overview of the Module 12
Leak and Waste Concept 14
Leak Sensor 15
This chapter gives an introduction to the module and an instrument overview.

1290 Infinity II Preparative Open-Bed Sampler/Collector User Manual 9
1Introduction
Intended Use
Intended Use
The 1290 Infinity II Preparative Open-Bed Sampler/Collector is designed to be
used in combination with other InfinityLab LC modules in a preparative-scale
HPLC system to separate and purify inorganic or organic compounds via
reversed-phase chromatography applications. The sampler can hold or store
samples in containers such as vials, tubes, or well plates, draw a specified
sample volume, and inject it into the reversed-phase HPLC system. The collector
can collect fractions of desired volume for sample purification or for later
analysis.
The product is intended to be used by professional laboratory users.

1290 Infinity II Preparative Open-Bed Sampler/Collector User Manual 10
1Introduction
Product Description
Product Description
The 1290 Infinity II Preparative Open-Bed Sampler/Collector is the solution for
automated, high-capacity management of sample injection and fraction
collection in semipreparative and preparative-scale LC purification workflows.
The X-Z-theta axis probe uses novel robotic technology to minimize sample
carryover during movements between fraction containers. Sample volumes can
be injected from microtiter plates, vials, or vessels.
The sampler/collector provides for injection of large volumes and sample loops
are available with volumes of 2, 5, 10, and 20 mL. The sampler/collector is
equipped with an analytical-to-preparative injector and enables easy handling of
a wide variety of collection vessels with a total capacity up to 5.9 L using 150 x
25 mm (l x od) tubes. Optimized for flow rates up to 200 mL/min, the
sampler/collector can be integrated seamlessly with any Agilent solvent delivery
module, recovery collector, or detector.
Figure 1 Overview of the Preparative Open-Bed Sampler/Collector
Container
Drawer
Power switch
Status indicator
Sampling assembly

1290 Infinity II Preparative Open-Bed Sampler/Collector User Manual 11
1Introduction
Features
Features
•Fully automated and combined open-bed autosampler and fraction collector
with capacity to inject up to 864 samples or collect up to 432 fractions, or
combinations of both, in a single module to optimize bench space.
•All sample and fraction vessels offer container recognition by identification
tags and are easily accessible and can be exchanged during operation
without stopping the instrument.
•One injector with two sample loops allows convenient switching between
preparative scale and analytical scale (scouting runs) without the need for
hardware modifications.
•Automated sample dilution prior to injection and fraction mixing for reanalysis
to support your analytical to preparative workflow.
•Supports 2, 5, 10, and 20 mL sample loops to increase sample loading and
your productivity by reducing the number of injections.
•The X-Z-theta axis probe uses novel robotic technology to minimize sample
carryover during movements between fraction containers, providing
compatibility with existing purification methods.
•Integration of all standard collection modes, plus the proprietary delay volume
sensor and automated calibration procedure, ensure optimized sample
recovery.
•Flexible bed layout allows sample injection from the vessel types, and fraction
collection into the vessel types best suited for your application.
•Preconfigured tubing kits with identification tags allow easy setup and provide
optimized substance recovery with minimized peak dispersion at any flow
rate.
•Forced fume extraction in combination with the bottom fume hood enables
use of the sampler/collector outside a fume cupboard.

1290 Infinity II Preparative Open-Bed Sampler/Collector User Manual 12
1Introduction
Overview of the Module
Overview of the Module
The Preparative Open-Bed Sampler/Collector combines a preparative sampler
with a preparative fraction collector in only one module. Figure 2 on page 12
gives an overview of the main module assemblies. Figure 3 on page 13 shows
the sampling assembly and solvent distribution assembly in more detail. The
sampling needle, washable needle guide, and fraction valve are located in the
robot arm. The wash port assembly consists of draw port, wash port, and nine
vial positions.
Figure 2 Overview of the module
Sampling assembly
Solvent distribution assembly
Robotics
Wash port assembly

1290 Infinity II Preparative Open-Bed Sampler/Collector User Manual 13
1Introduction
Overview of the Module
Figure 3 Overview of the sampling assembly and solvent distribution assembly
Sampling assembly
Metering pump
Injection valve
Solvent selection valves
Preparative loop
Analytical loop
Solvent distribution
assembly
Solvent selection
valves
Solvent divider
Peristaltic pumps

1290 Infinity II Preparative Open-Bed Sampler/Collector User Manual 14
1Introduction
Leak and Waste Concept
Leak and Waste Concept
1Wash port assembly 2Sampler leak plane
3Leak plane holder 4Drain Outlet

1290 Infinity II Preparative Open-Bed Sampler/Collector User Manual 15
1Introduction
Leak and Waste Concept
Leak Sensor
Figure 4 Sampler leak sensor
Figure 5 Position of leak tray and leak sensor
Leak tray
Leak sensor

1290 Infinity II Preparative Open-Bed Sampler/Collector User Manual 16
1Introduction
Leak and Waste Concept
CAUTION
Solvent incompatibility
The solvent DMF (dimethyl formamide) leads to corrosion of the leak sensor.
The material of the leak sensor, PVDF (polyvinylidene fluoride), is incompatible
with DMF.
Do not use DMF as mobile phase.
Check the leak sensor regularly for corrosion.

1290 Infinity II Preparative Open-Bed Sampler/Collector User Manual 17
2 Site Requirements and Specifications
Site Requirements 18
Exhaust and Vapour Management 21
Waste Management 22
Physical Specifications 23
Performance Specifications 24
This chapter provides information on environmental requirements, physical and
performance specifications.

1290 Infinity II Preparative Open-Bed Sampler/Collector User Manual 18
2 Site Requirements and Specifications
Site Requirements
Site Requirements
A suitable environment is important to ensure optimal performance of the
instrument.
Power Considerations
The module power supply has wide ranging capability. It accepts any line voltage
in the range described in Table 1 on page 23. Consequently there is no voltage
selector in the rear of the module. There are also no externally accessible fuses,
because automatic electronic fuses are implemented in the power supply.
WARNING
Hazard of electrical shock or damage of your instrumentation
can result, if the devices are connected to a line voltage higher than
specified.
Connect your instrument to the specified line voltage only.
WARNING
Electrical shock hazard
The module is partially energized when switched off, as long as the power
cord is plugged in.
The cover protects users from personal injuries, for example electrical
shock.
Do not open the cover.
Do not operate the instrument and disconnect the power cable in case
the cover has any signs of damage.
Contact Agilent for support and request an instrument repair service.
WARNING
Inaccessible power plug.
In case of emergency it must be possible to disconnect the instrument
from the power line at any time.
Make sure the power connector of the instrument can be easily reached
and unplugged.
Provide sufficient space behind the power socket of the instrument to
unplug the cable.

1290 Infinity II Preparative Open-Bed Sampler/Collector User Manual 19
2 Site Requirements and Specifications
Site Requirements
Power Cords
Country-specific power cords are available for the module. The female end of all
power cords is identical. It plugs into the power-input socket at the rear. The male
end of each power cord is different and designed to match the wall socket of a
particular country or region.
Agilent makes sure that your instrument is shipped with the power cord that is
suitable for your particular country or region.
WARNING
Unintended use of power cords
Using power cords for unintended purposes can lead to personal injury or
damage of electronic equipment.
Never use a power cord other than the one that Agilent shipped with this
instrument.
Never use the power cords that Agilent Technologies supplies with this
instrument for any other equipment.
Never use cables other than the ones supplied by Agilent Technologies
to ensure proper functionality and compliance with safety or EMC
regulations.
WARNING
Absence of ground connection
The absence of ground connection can lead to electric shock or short
circuit.
Never operate your instrumentation from a power outlet that has no
ground connection.
WARNING
Electrical shock hazard
Solvents may damage electrical cables.
Prevent electrical cables from getting in contact with solvents.
Exchange electrical cables after contact with solvents.

1290 Infinity II Preparative Open-Bed Sampler/Collector User Manual 20
2 Site Requirements and Specifications
Site Requirements
Bench Space
The module dimensions and weight (see Table 1 on page 23) allow you to place
the module on almost any desk or laboratory bench. It needs an additional
2.5 cm (1.0 inches) of space on either side and approximately 8 cm (3.1 inches)
in the rear for air circulation and electric connections.
If the bench shall carry a complete HPLC system, make sure that the bench is
designed to bear the weight of all modules.
The module should be operated in a horizontal position.
Condensation
CAUTION
Condensation within the module
Condensation can damage the system electronics.
Do not store, ship or use your module under conditions where temperature
fluctuations could cause condensation within the module.
If your module was shipped in cold weather, leave it in its box and allow it to
warm slowly to room temperature to avoid condensation.