Agilent Technologies InfinityLab LC Series User manual

Vialsamplers
Agilent InfinityLab LC Series
User Manual
Agilent InfinityLab LC Series Vialsamplers User Manual

Agilent InfinityLab LC Series Vialsamplers User Manual
Notices
Document Information
Document No: SD-29000242 Rev. E
Part No: G7129-90010 Rev. E
EDITION 04/2020
Copyright
© Agilent Technologies, Inc. 2015-2020
No part of this manual may be repro-
duced in any form or by any means
(including electronic storage and retrieval
or translation into a foreign language)
without prior agreement and written con-
sent from Agilent Technologies, Inc. as
governed by United States and interna-
tional copyright laws.
Agilent Technologies
Hewlett-Packard-Strasse 8
76337 Waldbronn
Warranty
The material contained in this document
is provided “as is,” and is subject to being
changed, without notice, in future edi-
tions. Further, to the maximum extent
permitted by applicable law, Agilent dis-
claims all warranties, either express or
implied, with regard to this manual and
any information contained herein, includ-
ing but not limited to the implied warran-
ties of merchantability and fitness for a
particular purpose. Agilent shall not be lia-
ble for errors or for incidental or conse-
quential damages in connection with the
furnishing, use, or performance of this
document or of any information con-
tained herein. Should Agilent and the user
have a separate written agreement with
warranty terms covering the material in
this document that conflict with these
terms, the warranty terms in the separate
agreement shall control.
Technology Licenses
The hardware and/or software described
in this document are furnished under a
license and may be used or copied only in
accordance with the terms of such
license.
Restricted Rights Legend
U.S. Government Restricted Rights. Soft-
ware and technical data rights granted to
the federal government include only
those rights customarily provided to end
user customers. Agilent provides this
customary commercial license in Soft-
ware and technical data pursuant to FAR
12.211 (Technical Data) and 12.212
(Computer Software) and, for the Depart-
ment of Defense, DFARS 252.227-7015
(Technical Data - Commercial Items) and
DFARS 227.7202-3 (Rights in Commer-
cial Computer Software or Computer
Software Documentation).
Safety Notices
CAUTION
A CAUTION notice denotes a
hazard. It calls attention to an
operating procedure, practice, or
the like that, if not correctly per-
formed or adhered to, could result
in damage to the product or loss
of important data. Do not proceed
beyond a
CAUTION notice until
the indicated conditions are fully
understood and met.
WARNING
A WARNING notice denotes a
hazard. It calls attention to an
operating procedure, practice, or
the like that, if not correctly per-
formed or adhered to, could
result in personal injury or death.
Do not proceed beyond a WARN-
ING notice until the indicated
conditions are fully understood
and met.

Agilent InfinityLab LC Series Vialsamplers User Manual 3
In This Book:
This manual covers the following modules:
•Agilent 1260 Infinity II Vialsampler (G7129A)
•Agilent 1290 Infinity II Vialsampler (G7129B)
•Agilent 1260 Infinity II Vialsampler (G7129C)
1Introduction
This chapter gives an introduction to the module and instrument overview.
2 Site Requirements and Specifications
This chapter provides information on environmental requirements, physical and
performance specifications.
3 Using the Module
This chapter provides information on how to use the module.
4 Preparing the Module
This chapter explains the operational parameters of the module.
5 Optimizing Performance
This chapter provides information on how to optimize the module.
6Troubleshootingand Diagnostics
This chapter gives an overview of the maintenance, troubleshooting, and
diagnostic features available for the Agilent InfinityLab Series Vialsampler.
7ErrorInformation
This chapter describes the meaning of error messages, and provides information
on probable causes and suggested actions how to recover from error conditions.

Agilent InfinityLab LC Series Vialsamplers User Manual 4
8 Maintenance
This chapter describes the maintenance of the module.
9 Parts and Materials for Maintenance
This chapter provides information on parts for maintenance.
10 Identifying Cables
This chapter provides information on cables used with the Agilent 1200 Infinity
Series modules.
11 Hardware Information
This chapter describes the detector in more detail on hardware and electronics.
12 LAN Configuration
This chapter provides information on connecting the module to the Agilent
ChemStation PC.
13 Appendix
This chapter provides additional information on safety, legal and web.

Agilent InfinityLab LC Series Vialsamplers User Manual 5
Contents
1Introduction9
Product Description (G7129A) 10
Features (G7129A) 11
Product Description (G7129B) 12
Features (G7129B) 13
Product Description (G7129C) 14
Features (G7129C) 15
Overview of the Module 16
Operating Principle 18
Leak and Waste Handling 27
2 Site Requirements and Specifications 33
Site Requirements 34
Physical Specifications 38
Performance Specifications 39
Specifications of the Sample Cooler 47
Specifications of the Sample Thermostat 49
Specifications of the Integrated Column Compartment 51
3 Using the Module 53
Magnets 55
Turn on/off 56
Status Indicators 58
Vial Drawers and Trays 59
Choice of Vials and Caps 70
Install the Optional Integrated Column Compartment 74
Using the Optional Integrated Column Compartment 96
Install the Optional Sample Cooler/Sample Thermostat 116
Using the Optional Sample Cooler/Sample Thermostat 123
Transporting the Sampler 132
Agilent Local Control Modules 137

Agilent InfinityLab LC Series Vialsamplers User Manual 6
4 Preparing the Module 139
Leak and Waste Handling 140
Preparing the Module 141
Solvent Information 142
Capillary Color Coding Guide 149
Installing Capillaries 150
Flow Connections to the Vialsampler 152
Setting up the Vialsampler 154
5 Optimizing Performance 166
Optimization for Lowest Carryover 167
Fast Injection Cycle and Low Delay Volume 171
Precise Injection Volume 173
Choice of Rotor Seal 175
6 Troubleshooting and Diagnostics 176
Status Indicators 177
Overview of Tests and Tools 178
Maintenance and Troubleshooting Tools 179
Diagnostic Tests 187
Agilent Lab Advisor Software 189
7ErrorInformation190
What are Error Messages 192
General Error Messages 193
Vialsampler Error Messages 201
Sample Cooler/Sample Thermostat Error Messages 213
Integrated Column Compartment (ICC) Heater Error Messages 219

Agilent InfinityLab LC Series Vialsamplers User Manual 7
8 Maintenance 222
Introduction to Maintenance 223
Warnings and Cautions 224
Overview of Maintenance 226
Cleaning the Module 227
Remove and Install Doors 228
Exchange the Needle Assembly 229
Exchange the Needle Seat Assembly 235
Exchange the Sample Loop Assembly 241
Exchange the Rotor Seal 245
Exchange the Metering Seal and Piston 250
Replace the Analytical Heads 255
Exchange the Gripper Arm 258
Replace the Peristaltic Pump Cartridge 261
Exchange the Wash Port Assembly 263
Replace the Module Firmware 269
Replace the Sample Cooler/Sample Thermostat 270
9 Parts and Materials for Maintenance 275
Standard Parts For Maintenance 276
Accessory Kits 279
Vial Drawers and Trays 283
Multidraw Kits 286
Sample Thermostat Upgrade 287
Integrated Column Compartment 288
Column ID Upgrade Kit 289
Parts for 900 µL Injection Upgrade 290
Cabinet and Door Kits 291
Analytical Head Assembly (100 µL) 295
Analytical-Head Assembly (900 µL) 296
Analytical Head Assembly (40 μL) 297
2 Position/6 Port Injection Valve, 600 bar 298
2 Position/6 Port Injection Valve, 800 bar 299
2 Position/6 Port Injection Valve, 1300 bar 300

Agilent InfinityLab LC Series Vialsamplers User Manual 8
10 Identifying Cables 301
Cable Overview 302
Analog Cables 304
Remote Cables 306
CAN/LAN Cables 310
RS-232 Cables 311
USB 312
11 Hardware Information 313
Firmware Description 314
Electrical Connections 317
Interfaces 319
Setting the 6-bit Configuration Switch 326
Instrument Layout 330
Early Maintenance Feedback (EMF) 331
12 LAN Configuration 332
What You Have to Do First 333
TCP/IP parameter configuration 334
Configuration Switches 335
Initialization Mode Selection 336
Dynamic Host Configuration Protocol (DHCP) 338
Manual Configuration 341
PC and Agilent ChemStation Setup 345
13 Appendix 354
General Safety Information 355
Waste Electrical and Electronic Equipment (WEEE) Directive 361
Refrigerant 362
Radio Interference 365
Sound Emission 366
Solvent Information 367
Agilent Technologies on Internet 368

Agilent InfinityLab LC Series Vialsamplers User Manual 9
1Introduction
Product Description (G7129A) 10
Features (G7129A) 11
Product Description (G7129B) 12
Features (G7129B) 13
Product Description (G7129C) 14
Features (G7129C) 15
Overview of the Module 16
Operating Principle 18
Sampling Sequence 18
Needle Parkstation 23
Hydraulic Box 24
Transport Assembly 26
Leak and Waste Handling 27
Leak Sensor 31
Waste Guidance 31
Waste Concept 32
This chapter gives an introduction to the module and instrument overview.

Agilent InfinityLab LC Series Vialsamplers User Manual 10
1Introduction
Product Description (G7129A)
Product Description (G7129A)
The Agilent 1260 Infinity II Vialsampler is an autosampler designed for the
reliability and ease-of-use needed for routine pharmaceutical tasks and quality
control, as well as for environmental and food analyses. It can house optionally
the integrated column compartment for two LC columns with temperature
control up to 80 °C as well as a sample thermostat for stable temperatures from
4°C to 40 °C, all within one module.
Figure 1 Overview of the Vialsampler
Status indicator
Needle assembly
Drawer
Column shelf
Leak drain
Power switch

Agilent InfinityLab LC Series Vialsamplers User Manual 11
1Introduction
Features (G7129A)
Features (G7129A)
• High capacity – up to 132 vials (2 mL) or up to 36 vials (6 mL).
• Reliable injections – 0.1 to 100 µL injections for up to 600 bar (G7129A) and
up to 800 bar (G7129C).
• Easy volume extensions – for injection volumes up to 1800 µL for applications
ranging from microbore to semipreparative chromatography.
•Lowestcarryover– with an in needle flush port included, for rinsing the outside
of the needle.
• Efficient temperature control – with an integrated column compartment as
option or upgrade available. Which holds two columns up to 30 cm length,
and provides heating capacity from 5 °C above ambient up to 80 °C for
reproducible chromatography data at optimized resolution.
• Integrated sample thermostat – available as option or upgrade, providing
cooling and heating in the range from 4 °C - 40 °C.
• Low internal volume – for minimum contribution to a system's total internal
volume, which can be even further reduced using "bypass" mode.
•Increasedproductivity– with overlapped injections.
• Customizable injection program – available for customizing advanced
injections as well as for sample preparation steps upfront injection.

Agilent InfinityLab LC Series Vialsamplers User Manual 12
1Introduction
Product Description (G7129B)
Product Description (G7129B)
The Agilent 1290 Infinity II Vialsampler (G7129B) is an autosampler designed for
UHPLC applications up to 1300 bar. It provides the reliability, safety, and
ease-of-use needed for routine pharmaceutical tasks and quality control, as well
as for environmental and food analyses. It can house optionally the integrated
column compartment for two LC columns with temperature control up to 80 °C
as well as a sample thermostat for stable temperatures from 4 °C to 40 °C, all
within one module.
Figure 2 Overview of the Vialsampler
Status indicator
Needle assembly
Drawer
Column shelf
Leak drain
Power switch

Agilent InfinityLab LC Series Vialsamplers User Manual 13
1Introduction
Features (G7129B)
Features (G7129B)
• Accurate and precise injections - within a wide and flexible range of volumes
• High capacity - up to 132 vials (2 mL) or up to 36 vials (6 mL).
• Easy volume extensions - for injection volumes up to 1500 µL for applications
ranging from microbore to semipreparative chromatography.
•Lowestcarryover- with an in needle flush port included, for rinsing the outside
of the needle.
• Efficient temperature control - with an integrated column compartment as
option or upgrade available. Which holds two columns up to 30 cm length,
and provides heating capacity from 5 °C above ambient up to 80 °C for
reproducible chromatography data at optimized resolution.
• Integrated sample thermostat - available as option or upgrade, providing
cooling and heating in the range from 4 – 40 °C.
• Low internal volume - for minimum contribution to a system's total internal
volume, which can be even further reduced using bypass mode.
•Increasedproductivity- with overlapped injections.
• Customizable Injection program - available for customizing advanced
injections as well as for sample preparation steps upfront injection.

Agilent InfinityLab LC Series Vialsamplers User Manual 14
1Introduction
Product Description (G7129C)
Product Description (G7129C)
The Agilent 1260 Infinity II Vialsampler is an autosampler designed for the
reliability and ease-of-use needed for routine pharmaceutical tasks and quality
control, as well as for environmental and food analyses. It can house optionally
the integrated column compartment for two LC columns with temperature
control up to 80 °C as well as a sample thermostat for stable temperatures from
4°C to 40 °C, all within one module.
Figure 3 Overview of the Vialsampler
Status indicator
Needle assembly
Drawer
Column shelf
Leak drain
Power switch

Agilent InfinityLab LC Series Vialsamplers User Manual 15
1Introduction
Features (G7129C)
Features (G7129C)
• High capacity – up to 132 vials (2 mL) or up to 36 vials (6 mL).
• Reliable injections – 0.1 to 100 µL injections for up to 600 bar (G7129A) and
up to 800 bar (G7129C).
• Easy volume extensions – for injection volumes up to 1800 µL for applications
ranging from microbore to semipreparative chromatography.
•Lowestcarryover– with an in needle flush port included, for rinsing the outside
of the needle.
• Efficient temperature control – with an integrated column compartment as
option or upgrade available. Which holds two columns up to 30 cm length,
and provides heating capacity from 5 °C above ambient up to 80 °C for
reproducible chromatography data at optimized resolution.
• Integrated sample thermostat – available as option or upgrade, providing
cooling and heating in the range from 4 °C - 40 °C.
• Low internal volume – for minimum contribution to a system's total internal
volume, which can be even further reduced using "bypass" mode.
•Increasedproductivity– with overlapped injections.
• Customizable injection program – available for customizing advanced
injections as well as for sample preparation steps upfront injection.

Agilent InfinityLab LC Series Vialsamplers User Manual 16
1Introduction
Overview of the Module
Overview of the Module
The Agilent InfinityLab LC Series Vialsampler is designed for use with other
modules of the Agilent InfinityLab LC Series, 1200 Series, and 1100 Series LC, as
well as with other LC systems with adequate remote-control capabilities. The
Vialsampler can be controlled from any computer with a suitable
chromatography data system (for example, Agilent OpenLab CDS, MassHunter,
etc.) or via an Agilent local control module (InfinityLab Companion or 1200
Infinity Series Instant Pilot).
The 1290 Infinity II Vialsampler (G7129B) can be operated at up to 1300 bar. The
two 1260 Infinity II Vialsampler variants (G7129A and G7129C) are limited to a
maximum system pressure of 600 and 800 bar, respectively.
The Vialsampler can accommodate two vial drawers, providing space for up to
132 samples. The two cartesian vial drawers allow for the use of either 66 × 2 mL
or 18 × 6 mL vials. A pair of classic vial drawers with a numerical vial assignment
system is also available, each capable of hosting 50 × 2 mL vials. The classic vial
drawers are designed to ease method transfer from older Agilent samplers. The
Vialsampler can also be equipped with an external tray, which can be beneficial
for automation. The Agilent WalkUp solution fully supports the external tray.
The Vialsampler features a robotic gripper arm for the transport of vials, driven by
four stepper motors to ensure the highest precision and flexibility for the
transport movement.
The default configuration of the analytical head and sample loop allows an
injection range from 0.1 – 20 μL for the 1290 Infinity II Vialsampler and
0.1 – 100 μL for the 1260 Infinity II Vialsamplers. An analytical head with
extended volume is also available for injection volumes from 0.1 – 900 μL, and
can be operated at up to 400 bar. With the Multidraw Kit, the maximum injection
volume can even reach 1800 μL.
The 2-position/6-port injection valve is driven by a high-speed, hybrid stepper
motor, ensuring smooth switching between the mainpass and bypass positions.
Thanks to the flow-through design of the Vialsampler, the eluent continuously
rinses the component parts of the sample introduction system. This, together
with the automated needle wash function for rinsing the outer surface of the
needle, ensures the lowest level of carryover for each analysis. For advanced
delay volume reduction, the injection valve can be programmed to automatically
switch back to the bypass position when the injection is completed.

Agilent InfinityLab LC Series Vialsamplers User Manual 17
1Introduction
Overview of the Module
The Agilent InfinityLab Integrated Column Compartment, an optional upgrade for
the Vialsampler, eliminates the need for having a standalone column thermostat
in the LC stack. It has the heating capability of reaching temperatures up to 80 °C
and can hold a total of two columns with maximum lengths of 30 cm.
For applications requiring control over the vial temperature, the Vialsampler can
be equipped with the Agilent InfinityLab Sample Thermostat (G7167-60101). It
features a vapor-compression refrigeration system and an electric heater,
allowing the Vialsampler to reach vial temperatures down to 4 °C and up to 40 °C.

Agilent InfinityLab LC Series Vialsamplers User Manual 18
1Introduction
Operating Principle
Operating Principle
Sampling Sequence
The Vialsampler processor continuously monitors the movements of the
vialsampler components during the sampling sequence. The processor defines
specific time windows and mechanical ranges for each movement. If a specific
step of the sampling sequence can’t be completed successfully, an error
message is generated.
During the sampling sequence, the solvent bypasses the vialsampler via the
injection valve. The gripper arm selects the sample vial, either from a static
sample rack, or from external vial positions. The gripper arm places the sample
vial below the injection needle. The required volume of sample is drawn into the
sample loop by the metering device. Sample is applied to the column when the
injection valve returns to the mainpass (main path) position at the end of the
sampling sequence.
The sampling sequence occurs in the following order:
1The injection valve switches to the bypass position.
2The piston of the metering device moves to the initialization position.
3The gripper arm moves from the home position, and selects the vial. At the
same time, the needle lifts out of the seat.
4The gripper moves into the needle station and stops in the draw position.
1
5The needle lowers into the vial.
6The metering device draws the defined sample volume.
7The needle lifts out of the vial.
8The gripper arm moves out slightly and stops in the wash position
1
.
9The needle moves downwards and dips into the wash well of the wash port.
Simultaneously the peristaltic pump delivers the flush solvent.
1
10 The needle moves back.
1
11 The gripper arm moves out of the needle station and the wash port snaps
back in position.
1
only if automated needle wash is selected. If this feature is disabled, the gripper arm
positions the sample vial directly below the needle (Step 4) and lowers the needle into
the vial.

Agilent InfinityLab LC Series Vialsamplers User Manual 19
1Introduction
Operating Principle
12 The gripper arm replaces the vial, and returns to the home position.
Simultaneously, the needle lowers into the seat.
13 The injection valve switches to the mainpass (main path) position.
Injection Sequence
Before the start of the injection sequence, and during an analysis, the injection
valve is in the mainpass (main path) position (see Figure 4 on page 19). In this
position, the mobile phase flows through the autosamplers metering device,
sample loop, and needle. This ensures that all parts in contact with sample are
flushed during the run, thus minimizing carryover.
Figure 4 Mainpass (main path) Position - standard position during runs and when the sampler
is idle
Vial Wash
port
Needle
seat
Sampling loop
Solvent
Wash
pump
Metering
device

Agilent InfinityLab LC Series Vialsamplers User Manual 20
1Introduction
Operating Principle
When the sample sequence begins, the valve unit switches to the bypass
position (see Figure 5 on page 20). Solvent from the pump enters the valve unit at
port 1, and flows directly to the column through port 6.
Figure 5Valveinbypass- needle in vial, metering device aspirates sample volume
Vial Wash
port
Needle
seat
Sampling loop
Solvent
Wash
pump
Metering
device