BANDELIN LABOCOOL LC 400 User manual

Operating Instructions
LABOCOOL
Laboratory cooler LC 400
51816-004 en/2023-12

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© 2023
1 2 GmbH & Co. kg, Heinrichstr. 3–4, 12207 Berlin, Germany
Certified to ISO 9001 and ISO 13485

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Table of Contents
1 About these operating instructions .................................................................................................... 5
2 Safety ...................................................................................................................................................... 6
2.1 Using the device ...................................................................................................................................6
2.2 Keep out of reach of children ............................................................................................................6
2.3 Risk of electric shock ..........................................................................................................................6
2.4 Danger due to refrigerant .................................................................................................................7
2.5 Disposal of the contact liquid ..........................................................................................................7
2.6 Preventing damage to the device ..................................................................................................7
2.7 Interference with wireless communication .................................................................................7
2.8 Safety stickers on the device ...........................................................................................................8
3 Construction and function .................................................................................................................... 9
3.1 Construction ..........................................................................................................................................9
3.2 Control panel ...................................................................................................................................... 10
3.3 Status and error messages ............................................................................................................ 11
3.4 Function ............................................................................................................................................... 11
4 Preparing for operation .....................................................................................................................12
4.1 Scope of delivery ............................................................................................................................... 12
4.2 Installation site requirements ....................................................................................................... 13
4.3 Installation .......................................................................................................................................... 13
4.4 Assembly ............................................................................................................................................. 14
5 Operation ..............................................................................................................................................16
5.1 Putting into service .......................................................................................................................... 16
5.2 Switching the device on and off .................................................................................................. 16
5.3 Pouring in the contact liquid .......................................................................................................... 17
5.4 Setting parameters .......................................................................................................................... 18
5.4.1 Setting the temperature ................................................................................................................. 18
5.5 Notes on sonication ........................................................................................................................ 18
5.6 Troubleshooting ................................................................................................................................. 19

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6 Maintenance .........................................................................................................................................20
6.1 Cleaning the housing ....................................................................................................................... 20
6.2 Rinsing the liquid circuit .................................................................................................................. 20
6.3 Servicing .............................................................................................................................................. 21
6.3.1 On-site servicing ............................................................................................................................... 21
6.4 Storage/safekeeping ........................................................................................................................ 22
6.5 Emptying the system ...................................................................................................................... 22
6.6 Repairs ................................................................................................................................................. 23
7 Disposal ................................................................................................................................................24
8 Information about the device ............................................................................................................25
8.1 Technical data .................................................................................................................................... 25
8.2 Ambient conditions .......................................................................................................................... 26
8.3 CE conformity ..................................................................................................................................... 26
9 Accessories ...........................................................................................................................................27

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About these operating instructions
1 About these operating instructions
These operating instructions contain information that is necessary and useful in order to use
the device safely and efficiently.
• Before using the device, read these operating instructions.
• Pay particular attention to chapter 2 Safety.
• If you pass on this device, provide these operating instructions with it.
• Contact your dealer or BANDELIN if any questions in these operating instructions are not
answered. Notes on service can be found in chapter 6.6 Repairs.
In the event that the translation cannot be understood, the German original version of
BANDELIN must be followed.
BANDELIN assumes no responsibility or liability for damage caused by improper handling or
use.
Illustrations are exemplary and not to scale. Decorations not included with delivery.

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2 Safety
2.1 Using the device
The LABOCOOL laboratory cooler is used to cool aqueous fluids in open ultrasonic baths.
The LABOCOOL laboratory cooler is suitable for ultrasonic baths or other vessels with an
outlet at the base.
The device is intended for commercial use and is designed for unobserved continuous
operation.
The target group for use is trained specialists in laboratories or research institutions.
2.2 Keep out of reach of children
Children may not detect hazards emanating from the device. Therefore, keep the device
away from children.
2.3 Risk of electric shock
The device is an electrical device. Failure to follow safety rules can result in a life-threatening
electric shock.
• Protect the device from moisture and wetness. Keep the surface and controls clean and
dry.
• Only transport the device when it is empty.
• Empty the device only when it is switched off.
• Do not rinse the appliance or expose it to splash water.
• Disconnect the appliance from the mains before any cleaning or maintenance operation.
• Only connect the device to a socket with an earthed protective contact that matches the
protective contact of the mains connector.
WARNING
Note for unit with type E+F jack:
Combination with socket type K (especially common in Denmark) is not permitted.
• If you notice a defect in the device, unplug the mains plug immediately. Do not connect a
defective device to the mains.
• Only have repairs carried out by qualified personnel or by the manufacturer. See chapter
6.6 Repairs.
• Position the device in such a way that it is possible to disconnect the mains connection at
any time without difficulty.
Safety

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2.4 Danger due to refrigerant
The integrated cooling unit contains a combustible refrigerant.
If the refrigerant circuit is damaged, the mains plug must be unplugged immediately and the
installation site must be well ventilated. Send the device to the manufacturer for repair.
For flawless operation, observe the following instructions:
• Refrigerant information:
R-290; quantity: 90 g, highly flammable
• Never cover or close the supply and exhaust air openings.
• To protect the cooling unit, the compressor is only started after a rest period of two
minutes.
2.5 Disposal of the contact liquid
Dispose of the contact liquid according to the instructions of the manufacturers of the
ultrasonic agents used. The recommended ultrasonic agents of the TICKOPUR product
series from DR. H. STAMM GmbH are biodegradable in accordance with the provisions of
Regulation (EC) No. 648/2004 (Detergents Regulation). If necessary, the contact liquid must
be neutralised before disposal.
Observe local sewage regulations.
2.6 Preventing damage to the device
• Do not expose laboratory coolers to direct sunlight and do not place them near sources of
heat.
• Do not place laboratory coolers in environments containing saline air.
• Do not operate the appliance without liquid
2.7 Interference with wireless communication
The device may interfere with other wireless communication devices in close proximity,
9such as:
• Mobile phones
• Wi-Fi devices
• Bluetooth devices
If a wireless device malfunctions, increase its distance from the device.
The device complies with the requirements for Class B devices according to EN 55011.
Safety

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3 Construction and function
3.1 Construction
1
2
3
4
5
6
Construction and function

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3.2 Control panel
8
9
7
1 Cover for the exhaust air opening (rotatable cover)
2 Handles, opening for transport (both sides)
3 Display, with control panel for setting the target temperature
4 Supply air opening, with filter
5 Main switch
6 Mains cable
7 Exhaust air opening
8 Inlet hose
9 Drain hose
1 2 3 45
1 Display
2 Arrow keys [], [] and On/Off
3 [SET] button
4 Display area – status and error messages
5 Display area – temperature
Construction and function

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3.3 Status and error messages
LED display area – status and error messages
LED Explanation
Lights up Device in cooling mode
Flashes Device ready for cooling
On Alarm condition
3.4 Function
With the integrated centrifugal pump, the bath liquid heated by the ultrasonic bath is
conveyed into a circuit through the LABOCOOL laboratory cooler and cooled to the desired
temperature. The integrated temperature controller allows the target temperature to be
set and the actual temperature to be displayed. The heated exhaust air is discharged on the
upper part of the device, either on the front or back or on one of the lateral surfaces of the
device.
The LABOCOOL laboratory cooler is operated via buttons on the front in on-table mode.
Construction and function

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4 Preparing for operation
After unpacking, check the laboratory cooler and the accessories for any transport damage
and to ensure they are complete. If you discover any damage or defects, report this/these
immediately in writing to the shipping carrier and the supplier.
Allow the device to adapt to the climatic conditions at the installation site for at least 2
hours.
4.1 Scope of delivery
Set comprising:
1× laboratory cooler, including connection set
Connection set comprising:
1 × inflow nozzle
1 × hose, 3 m (cut to size)
1 × 3-way ball valve with
2 hose sockets
1 × adapter with nut (for ¼" outlet)
1 × sealing tape
1 × tool
1 × operating instructions
Other accessories depending on the order – see delivery note.
Preparing for operation

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4.2 Installation site requirements
The installation site of the device must meet the following conditions:
• The installation surface must be horizontal, firm and dry.
• The load-bearing capacity must be sufficient for the device together with the contact
liquid and the device to be connected. For weight, see chapter 8.1 Technical data.
• Adequate ventilation must be ensured. The air supply of the device must not be impeded
by objects.
• Observe minimum distances for supply and exhaust air. At least 5 cm on the side and at
least 10 cm on the rear wall.
• A water connection for filling the device should be located nearby. A basin for draining or
pouring the contact liquid must be available.
• The cover can be loosened with the supplied tool and, if desired, turned and put back. This
allows the operator to choose the direction of the air flow. Operation without a cover is
not permitted.
4.3 Installation
The laboratory cooler can be placed next to or under the ultrasonic bath to be connected.
In any case, it should be noted that the drain hose from the connected ultrasonic bath to the
inlet of the laboratory cooler has a gradient, and the liquid can flow into the laboratory cooler
on its own.
The inlet of the laboratory cooler may be located up to one metre below the outlet, so the
laboratory cooler can also be placed under the work surface.
Preparing for operation

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4.4 Assembly
For proper function, the following instructions must be observed during installation.
Rear view:
NOTICE
No self-priming pump
The inlet of the laboratory cooler must be installed below the liquid level.
NOTICE
Sealing capacity of the sealing tape
Do not screw the adapter on up to the stop or unscrew it by turning it anti-clockwise, be-
cause this will cause the sealing ability of the sealing tape to be lost.
Assembly of the ultrasonic bath outlet
• Replace the existing ball valve at the outlet of the ultrasonic bath with the 3-way ball
valve with hose sockets.
• Remove the old sealing tape.
• Wrap several layers of sealing tape around the external thread.
• First fix the nut with the adapter at the ¼" outlet to the outlet pipe with sealing tape.
• Unscrew the 3-way ball valve.
• Likewise, wrap the hose sockets with sealing tape and attach them to the 3-way ball
valve.
• Cut the hose to the appropriate length and connect it between the laboratory cooler
and the 3-way ball valve.
The hose must not have kinks in it.
• Fix the outlet at the edge of the ultrasonic bath tank with the screw. Connect the hose
to the laboratory cooler.
Preparing for operation

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Preparing for operation
Assembly of the ultrasonic bath inlet
• Place the inlet nozzle on one corner of the ultrasonic bath and fix it in place with the
screws.
• Cut the hose to the appropriate length and connect it between the laboratory cooler
and the inlet nozzle.
The hose must not have kinks in it.

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5 Operation
5.1 Putting into service
For optimal transmission of ultrasound between the sound-emitting surface and the
reaction vessels, the ultrasonic bath must be filled with a contact liquid consisting of normal
municipal or purified water and a surfactant-containing agent, e.g., TICKOPUR TR 3.
Requirements
• The device must have adapted to the climatic conditions at the installation site for at least
2 hours.
Procedure
1. Connect the laboratory cooler to the earthed socket.
2. Connect the laboratory cooler to the inlets and outlets of the ultrasonic bath via the
hoses. See notes in chapter 4.4 Assembly.
Result
» The device is ready to switch on.
5.2 Switching the device on and off
Switching the device on
Switch on the laboratory cooler at the main switch on the side.
» After switching on, the display must light up.
» The last selected target temperature is stored and will be available when the device is
switched on again.
Switching the device off
Switch off the laboratory cooler at the main switch on the rear.
Operation

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Information
- To protect the cooling unit, the compressor is only started after a rest period of two min-
utes.
- The On/Off button on the display is used to switch off the cooling function. The pump
will remain switched on. "Off" will be shown on the display. To switch the device on or
off, the button must be pressed for a longer time.
- If no button is pressed for 30 seconds, all settings will be saved, and the laboratory cool-
er will be ready for operation.
5.3 Pouring in the contact liquid
Requirements
• The device must be properly connected.
Procedure
1. Dose the agent for the contact liquid in a container together with water.
2. Fill the ultrasonic bath. The liquid must not be contaminated.
3. Refill the liquid until the desired level is reached.
4. Switch on the laboratory cooler at the main switch – the pump starts immediately. It is
necessary to fill the ultrasonic bath during putting into service.
During operation, the level must be checked at regular intervals.
Result
» You can now use the ultrasonic bath.
Operation

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5.4 Setting parameters
5.4 .1 Setting the temperature
Requirements
• The device must have been switched on at the main switch.
Procedure
1. Press the "SET" button for 3 seconds. The current target value will be displayed, and
the °C temperature unit symbol begins to flash.
2. Use the and buttons to set the target temperature in the range of 5–30°C.
3. Confirm the set value by pressing the "SET" button.
Result
» The laboratory cooler will begin to cool depending on the selected temperature.
5.5 Notes on sonication
To achieve a good result, observe the following instructions when introducing objects for
sonication:
• Before each sonication process, check whether the contact liquid is contaminated. If there
is visible contamination, replace the contact liquid.
• Only use accessories approved by the manufacturer.
• Allow the ultrasound to be switched off while inserting or removing sample vessels.
• Remove air bubbles under the sample vessels. The ultrasound only acts where liquid has
contact with the object of sonication or the sample vessel.
Operation

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5.6 Troubleshooting
Overview of error messages issued:
Error Possible causes Troubleshooting
The display does not
switch on.
No power supply. Check that the plug is fully inserted into
the socket.
Inadequate water
cooling.
Insufficient water flow Check the filling level, the hose lines and
the function of the integrated pump.
Exhaust air from the
ventilation grille is at
room temperature.
Defective cooling compressor. Contact the
supplier to repair the device.
The message "HA2"
(overheating) ap-
pears on the display.
Air filter dirty Clean the air filter.
Ambient temperature
too high
Restore the necessary maximum permis-
sible ambient temperature (38°C).
The message "P1"
appears on the dis-
play.
Water temperature
sensor defective
Contact the supplier to repair the device.
The message "P2"
appears on the dis-
play.
Overheating tempera-
ture sensor defective
Contact the supplier to repair the device.
The message "HA"
appears on the dis-
play.
High water tempera-
ture
Check the filling level and the hose lines.
Check that the cooling function is
switched on.
The displayed tem-
perature is not the
actual one.
The water is not circu-
lating properly in the
circuit.
Check the filling level, the hose lines and
the function of the integrated pump.
The lines are too long. Shorten and insulate the lines as much as
possible.
Operation

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6 Maintenance
In the event of improper or irregular disinfection of the laboratory cooler, microbiological
contamination is possible due to the settlement of microorganisms, especially in the hoses,
which can lead to cross-contamination. Therefore, the liquid circuit and the device surface
must be regularly disinfected and cleaned.
6.1 Cleaning the housing
• Wipe the housing with a damp cloth. Wipe it dry with a soft cloth.
• Do not use abrasive cleaning agents; only care products without abrasive additives.
• If necessary, disinfect the housing with a suitable surface disinfectant.
6.2 Rinsing the liquid circuit
During operation, the circuit for the contact liquid runs from the outlet of the ultrasonic bath
via the pump to the cooling unit and, from there, back into the ultrasonic bath. For hygienic
reasons, this circuit must be rinsed regularly.
• Fill the ultrasonic bath with liquid.
• Switch on the laboratory cooler. The pump will start conveying and will rinse the cooling
unit.
• Allow the pump to run for at least 5 minutes.
• Switch off the laboratory cooler and empty it.
• Repeat the described rinsing process at least twice.
Maintenance
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