Bio-Tek 50 TS User manual

50™ TS Washer Instructions for Use
(For In Vitro Diagnostic Use)
April 2017
©April 2017
Part Number 1551010
Revision A
BioTek Instruments, Inc.

ii | Preface
50™ TS Washer
Notices
BioTekInstruments, Inc.
Highland Park, P.O. Box 998
Winooski, Vermont 05404-0998 USA
All Rights Reserved
© April 2017, BioTek®Instruments, Incorporated. No part of this publication
may be reproduced, transcribed, or transmitted in any form, or by any means
electronic or mechanical, including photocopying and recording, for any purpose
other than the purchaser’s use without written permission of BioTek
Instruments, Inc.
Trademarks
BioTek®is a registered trademark and 50™ TS Washer is a trademark of BioTek
Instruments, Inc.
Microsoft®, Internet Explorer®, Windows®, Windows 7, Windows 10, and Excel®
are either registered trademarks or trademarks of Microsoft Corporation in the
United States and/or other countries.
All other trademarks are the property of their respective holders.
Restrictions and Liabilities
Information in this document is subject to change and does not represent a
commitment by BioTek Instruments, Inc. Changes made to the information in
this document will be incorporated in new editions of the publication. No
responsibility is assumed by BioTek for the use or reliability of software or
equipment that is not supplied by BioTek or its affiliated dealers.

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BioTek Instruments, Inc.
Contact Information
BioTekInstruments, Inc.
Highland Park, P.O. Box 998
Winooski, Vermont 05404-0998 USA
Customer Service and Sales
Phone:
888-451-5171 (toll free in the U.S.)
802-655-4740 (outside the U.S.)
Fax:
802-655-7941
E-Mail:
[e-mail]
Service/TAC
Phone:
800-242-4685 (toll free in the U.S.)
802-655-4740 (outside the U.S.)
Fax:
802-654-0638
E-Mail:
[e-mail]
European Coordination Center/Authorized European Representative
BioTekInstruments GmbH
Internet:
www.biotek.de
Kocherwaldstrasse 34
Phone:
[phone]
D-74177 Bad Friedrichshall
Fax:
[phone]
Germany
E-Mail:
[e-mail]
Instructions for Use Requirements
This document fulfills the basic needs of persons operating this device, according to
the requirements of the In Vitro Diagnostic Directive for “Instructions for Use.” Some
of the device’s higher-level functions and features, as well as certain detailed
maintenance and qualification routines, are described in the 50™ TS Washer
Operator’s Manual.
Intended Use Statement
•The 50™ TS Washer provides microplate priming, dispensing, and washing for ELISA™,
fluorescence and chemiluminescence immunoassays, cellular and agglutination assays.

iv | Preface
50™ TS Washer
•If the instrument has an “IVD” label it may be used for clinical and non-clinical
purposes, including research and development. If there is no such label the instrument
may only be used for research and development or other non-clinical purposes
Quality Control
It is considered good laboratory practice to run laboratory samples according to
instructions and specific recommendations included in the assay package insert for
the test to be conducted. Failure to conduct Quality Control checks could result in
erroneous test data.
Warnings
Operate the instrument on a level, stable surface away from excessive humidity.
When operated in a safe environment, according to the instructions in this
manual, there are no known hazards associated with the 50™ TS Washer.
However, the operator should be aware of certain situations that could result in
serious injury. See Hazards and PrecautionsError! Bookmark not defined..
Strict adherence to instrument maintenance and qualification procedures is
required to ensure accurate dispense volumes and risk-free operation.

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BioTek Instruments, Inc.
Hazards and Precautions
Hazards
The following hazards are provided to help avoid injury:
Warning! Power Rating. The instrument’s power
supply or power cord must be
connected to a power receptacle that provides voltage and current within the
specified rating for the system. Use of an incompatible power receptacle may
produce electrical shock and fire hazards.
Warning! Electrical Grounding. Never use a plug adapter to connect primary
power to the external power supply. Use of an adapter disconnects the utility
ground, creating a severe shock hazard. Always connect the power cord directly
to an appropriate receptacle with a functional ground.
Warning! Service. Only qualified technical personnel should perform service
procedures on internal components.
Warning! Accessories. Only accessories which meet the manufacturer’s
specifications shall be used with the instrument.
Warning! Lubricants. Do not apply lubricants to the microplate carrier or
carrier track. Lubricant on the carrier mechanism will attract dust and other
particles, which may obstruct the carrier path and cause the instrument to
produce an error.

vi | Preface
50™ TS Washer
Warning! Liquids. Avoid spilling liquids on the instrument; fluid seepage into
internal components creates a potential for shock hazard or instrument damage.
If a spill occurs while a program is running, abort the program and turn the
instrument off. Wipe up all spills immediately. Do not operate the instrument if
internal components have been exposed to fluid.
Warning! Unspecified Use. Failure to operate this equipment according to the
guidelines and safeguards specified in this manual could result in a hazardous
condition.
Warning! Direct Drain Waste. If installed, the direct drain waste system
pumps waste fluids from the washer directly into a sink or tank, and, potentially
into public waste water systems. Because the waste may be a biohazard, you
must ensure that you are in compliance with your local or national government’s
laws regarding safe disposal of the waste.
Warning! Software Quality Control. The operator must follow the
manufacturer’s assay package insert when modifying software parameters and
establishing washing or dispensing methods. Failure to conduct quality
control checks could result in erroneous test data.
Warning! Internal Voltage. Always turn off the power switch and unplug the
power supply before cleaning the outer surface of the instrument.
Warning! Potential Biohazards. Some assays or specimens may pose a
biohazard. Adequate safety precautions should be taken as outlined in the
assay’s package insert. This hazard is noted by the symbol shown here. Always
wear safety glasses and appropriate protective equipment, such as chemically
resistant rubber gloves and apron.
Warning! Pinch Hazard. Do not reach under the instrument during operation;
the syringe pump may pinch your fingers.
Precautions
The following precautions are provided to help avoid damage to the instrument:
Caution: Service. The instrument should be serviced by BioTek authorized
service personnel. Only qualified technical personnel should perform
troubleshooting and service procedures on internal components.

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BioTek Instruments, Inc.
Caution: Spare Parts. Only approved spare parts should be used for
maintenance. The use of unapproved spare parts and accessories may result in a
loss of warranty and potentially impair instrument performance or cause damage
to the instrument.
Caution: Environmental Conditions. Do not expose the instrument to
temperature extremes. For proper operation, ambient temperatures should
remain within the range listed in the Specifications section. Performance may
be adversely affected if temperatures fluctuate above or below this range.
Storage temperature limits are broader.
Caution: Sodium Hypochlorite. Do not expose any part of the instrument to
the recommended diluted sodium hypochlorite solution (bleach) for more than 20
minutes. Prolonged contact may damage the instrument surfaces. Be certain to
rinse and thoroughly wipe all surfaces.
Caution: Buffer Solution. Although many precautions have been taken to
ensure that the instrument is as corrosion-proof as possible, the instrument is not
sealed and liquids can see
p into sensitive components. Make sure that any spilled
buffer solution is wiped off the instrument. Prolonged exposure to salt solution
may corrode parts of the microplate carrier, movement rail, springs, and other
hardware.
Caution: Chemical Compatibility. Some chemicals may cause irreparable
damage to the instrument. The following chemicals have been deemed safe for
use in the instrument: buffer solutions (such as PBS), saline, surfactants,
deionized water, 70% ethyl, isopropyl, or methyl alcohol, 40%
formaldehyde, and
20% sodium hydroxide. Never use acetic acid, DMSO, or other organic solvents.
These chemicals may cause severe damage to the instrument. Contact BioTek for
more information and prior to using other questionable chemicals.
Caution: Bovine Serum Albumin.
Solutions containing proteins, such as bovine
serum albumin (BSA), will compromise the instrument’s performance over time
unless a strict maintenance protocol is adhered to. See Maintenance procedures
regarding BSA.
Caution: Power Supply. Only use the power supply shipped with the
instrument. Operate this power supply within the range of line voltages listed on
it.
Caution: Disposal. Dispose of the instrument according to Directive
2012/19/EU, “on waste electrical and electronic equipment (WEEE)” or local
ordinances.
Caution: Warranty. Failure to follow preventive maintenance protocols may
void the warranty.
Caution: Shipping Hardware. All shipping hardware (e.g., shipping bracket
etc.) must be removed before operating the instrument and reinstalled before
repackaging the instrument for shipment.
Caution: Electromagnetic Environment. Per IEC 61326-2-6 it is the user’s
responsibility to ensure that a compatible electromagnetic environment for this
instrument is provided and maintained in order that the device will perform as

viii | Preface
50™ TS Washer
intended.
Caution: Electromagnetic Compatibility. Do not use this device in close
proximity to sources of strong electromagnetic radiation (e.g., unshielded
intentional RF sources), because these may interfere with the proper operation.
Caution: Use BioTek-Provided Bottles Only.
Do not substitute the fluid supply
and waste bottles provided by BioTek with other commercially-available bottles.
BioTek provides bottles that perform well with our liquid handling systems,
including the vacuum pressure of the waste system.
Caution: Touchscreen. Do not use sharp implements to operate the
touchscreen. Using a sharp stylus or any implement harder than a finger may
damage the display.
CE Mark
Based on the testing described below and information contained
herein, this instrument bears the CE mark.
Note: See the Declaration of Conformity for specific information.
Directive 2014/30/EU: Electromagnetic Compatibility
Emissions—Class A
The system has been type-tested by an independent, accredited testing laboratory
and found to meet the requirements of EN 61326-1: Class A for Radiated
Emissions and Line Conducted Emissions.
Verification of compliance was conducted to the limits and methods of EN 55011
(CISPR 11) Class A. In a domestic environment it may cause radio interference, in
which case, you may need to mitigate the interference.
Immunity
The system has been type-tested by an independent, accredited testing laboratory
and found to meet the requirements of EN 61326-1 and EN 61326-2-6 for
Immunity. Verification of compliance was conducted to the limits and methods of
the following:
EN 61000-4-2, Electrostatic Discharge
EN 61000-4-3, Radiated EM Fields
EN 61000-4-4, Electrical Fast Transient/Burst
EN 61000-4-5, Surge Immunity

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BioTek Instruments, Inc.
EN 61000-4-6, Conducted Disturbances from RFI
EN 61000-4-11, Voltage Dips, Short Interruptions and Variations
Directive 2014/35/EU Low Voltage (Safety)
The system has been type-tested by an independent testing laboratory and was
found to meet the requirements of this Directive. Verification of compliance was
conducted to the limits and methods of the following:
EN 61010-1, “Safety requirement for electrical equipment for measurement,
control and laboratory use. Part 1, General requirements.”
EN 61010-2-081, “Particular requirements for automatic and semi-automatic
laboratory equipment for analysis and other purposes.”
Directive 2012/19/EU: Waste Electrical and Electronic Equipment
Disposal Notice: Dispose of the instrument according to Directive 2012/19/EU,
“on waste electrical and electronic equipment (WEEE)” or local ordinances.
Directive 98/79/EC: In Vitro Diagnostics (if labeled for this use)
•Product registration with competent authorities.
•Traceability to the U.S. National Institute of Standards and Technology (NIST).
•EN 61010-2-101 Particular requirements for in vitro diagnostic (IVD) medical
equipment.

x | Preface
50™ TS Washer
Safety Symbols
Some of these symbols appear on the instrument or accessories:
Alternating current
Courant alternatif
Wechselstrom
Corrientealterna
Correntealternata
Both direct and alternating current
Courant continu et courant alternatif
Gleich - und Wechselstrom
Corriente continua y corrientealterna
Corrente continua e correntealternata
Direct current
Courant continu
Gleichstrom
Corriente continua
Corrente continua
Earth ground terminal
Bo, height,rne de terre
Erde (Betriebserde)
Borne de tierra
Terra (difunzionamento)
On (Supply)
Marche (alimentation)
Ein (VerbindungmitdemNetz)
Conectado
Chiuso
Protective conductor terminal
Borne de terre de protection
Schutzleiteranschluss
Borne de tierra de protección
Terra diprotezione
Off (Supply)
Arrêt (alimentation)
Aus (TrennungvomNetz)
Desconectado
Aperto
(sconnessionedallaretedialimentazi
one)
Caution (refer to accompanying
documents)
Attention (voir documents
d’accompanement)
AchtungsieheBegleitpapiere
Atención (vease los documentosincluidos)
Attenzione, consultare la doc annessa
Warning, risk of electric shock
Attention, risque de choc
électrique
Gefährlicheelektrischeschlag
Precaución, riesgo de
sacudidaeléctrica
Attenzione, rischiodiscossaelettrica
Warning, risk of crushing or pinching
Attention, risqued’écrasement et pincement
Warnen, Gefahr des Zerquetschens und
Klemmen
Precaución, riesgo del machacamiento y
sejeción
Attenzione, rischiodischiacciareedintrappolarsi
Warning, hot surface
Attention, surface chaude
Warnen, heißeOberfläche
Precaución, superficiecaliente
Attenzione, superficiecalda
Warning, potential biohazards
Attention, risquesbiologiquespotentiels
Warnung! MoeglichebiologischeGiftstoffe
Atención, riesgosbiológicos
Attenzione, rischiobiologico
In vitro diagnostic medical device
Dispositif médical de diagnostic in
vitro
Medizinisches In-Vitro-
Diagnostikum
Dispositivo médico de diagnóstico
in vitro
Dispositivo medico diagnostico in
vitro
Separate collection for electrical and
electronic equipment
Les équipements électriques et électroniques
font l’objet d’une collecte sélective
Getrennte Sammlung von Elektro- und
Elektronikgeräten
Recogida selectiva de aparatos eléctricos y
electrónicos
Raccolta separata delle apparecchiature
elettriche ed elettroniche

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BioTek Instruments, Inc.
Consult instructions for use
Consulter la notice d’emploi
Gebrauchsanweisung beachten
Consultar las instrucciones de uso
Consultare le istruzioni per uso

Section 2
Installation
This section provides detailed installation instructions.
Unpack and Inspect the Instrument................................................... 2
Setting Up the 50 TS .......................................................................... 3
Connect the Fluid Supply and Waste System...................................... 4
Install the Microplate Carrier ........................................................... 13
Attach the Mist Shield ...................................................................... 15

2 |
50™ TS Washer
Unpack and Inspect the Instrument
Important: Save all packaging materials. If the washer is shipped to the
factory for repair or replacement, it must be carefully repackaged in the original
packing materials. Using other forms of commercially available packing
materials, or failure to follow the repackaging instructions may void your
warranty. Improper packaging that results in damage to the instrument may
lead to additional charges
Inspect the shipping box, packaging, instrument, and accessories for signs of
damage.
If the washer is damaged: Notify the carrier and your BioTek representative.
Keep the shipping cartons and packing material for the carrier's inspection.
BioTek will arrange for immediate repair or replacement of your instrument.
Unpack the accessories and the washer. Washers are shipped with two or three
2-liter supply bottles and one 2-liter waste bottle. The 50 TS with Buffer
Switching (“V” version) includes three supply bottles.
Liquid Level Alert™
If you purchased the Liquid Level Alert system, its components are shipped in
a separate box.
•For washers with Buffer Switching (“V” version washers), PN 4070036 includes
a select box, three 2-liter supply bottles, and one 2-liter waste bottle.
•For washers without Buffer Switching, PN 4070035 includes a select box, one 2-
liter supply bottle, and one 2-liter waste bottle.

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BioTek Instruments, Inc.
Setting Up the 50 TS
Important: Avoid excessive humidity. Condensation directly on the sensitive
electronic circuits can cause the instrument to fail internal self checks.
Important: The manifold dispense tubes have a protective Teflon collar at the
tip. This is to prevent dripping. Do not remove these coverings!
Operating Environment
The 50 TS washer is sensitive to extreme environmental conditions. For
optimal operation, install the washer:
•on a stable, level surface,
•in an area where ambient temperatures between 15° - 40°C (59° - 104°F)
can be maintained, and
•away from excess humidity.

4 |
50™ TS Washer
Connect the Fluid Supply and Waste System
Caution. The washer manifold, the vacuum port, and the supply
bottles have Luer fittings. Finger-tighten only!!
Place the supply and waste bottles on the same horizontal plane as the
washer. This ensures optimum pump performance.
Liquid Level Alert System: If you purchased the optional level alert
accessory, use the special supply and waste bottles provided with the
kit during this installation procedure. Unlike the standard bottles, the
liquid level alert bottles have a sensing device inside, connected to an
electrical cable in the top. When the washer is installed, follow the
instructions to enable the system, See Install the Liquid Level Alert
System (Optional) on page 9.
Connect the Fluid Supply
Fluid Supply without Buffer
Switching is shown in this photo.
The tubing connects directly from
the bottle to the syringe pump to
the wash manifold.
1. Locate the tubing with the
check valves used for the
fluid supply, PN: 4070510.
2. Connect the short tube to
the top port of the syringe
pump and to the port on the
wash manifold nearest the
edge. Make sure the flow
arrow on the check valve
points towards the manifold.

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BioTek Instruments, Inc.
3. Connect the cut end of the short tube to the top port of the syringe
pump. Connect the Luer fitting end to the port on the wash manifold
closest to the edge. Make sure the flow arrow on the check valve points
towards the manifold.
4. Connect the long tube to the bottom port of the syringe pump. This is the
fluid supply "Input." Make sure the flow arrow points toward the washer.
5. Without Buffer Switching: Connect the long tube to the top of the supply
bottle.
With Buffer Switching: The tube will extend only from the syringe pump
to the Valve Output port next to the pump.
1. First, remove the Luer fitting on one end of the tube and cut the long
tube to about 5” (12.7 cm) from the check valve.
2. Reinsert the Luer fitting and connect it to the Valve Output port on the
back of the washer.
Fluid supply with Buffer Switching: tubing connects to internal valves

6 |
50™ TS Washer
5. Connect the three supply bottles, A, B, C, to their respective ports.

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BioTek Instruments, Inc.
Connect the Waste System
Finger-tighten only all fittings!
All 50 TS models except 8MF, 8M, 8F:
Connecting the Waste system tubing on the back of the washer
1. Remove the waste bottle cover and replace it with the stopper from the
waste tubing set.
2. Attach the Y-shaped tubing from the waste bottle stopper to the two waste
ports on the back of the wash manifold.
3. Install the inline vacuum filter:

8 |
50™ TS Washer
1. Cut the tubing that will connect the
waste bottle to the Vacuum port: leave
about an inch of tubing extending from
the Luer fitting that will connect with
the washer.
2.
Insert the vacuum filter between the cut
ends of the tubing.
The inline vacuum filter (PN: 48146)
is optional but strongly
recommended to (1) prevent waste
material aerosols from escaping into
the air, and (2) to serve as a
temporary fluid barrier if the waste
bottle is allowed to overfill.
4. (After inserting the inline vacuum filter) Connect the tubing from the waste
bottle to the Vacuum port on the back of the washer.
50 TS/8F, 8M, 8MF models (for vacuum filtration)
Installing the Waste system for vacuum filtration models: 8F, 8M, and 8MF models.
1. Attach the Y-shaped tubing to the two waste ports on the back of the wash
manifold (two center ports).
2. Connect the end of the Y-waste tubing to the Manifold port on the back of
the washer.

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BioTek Instruments, Inc.
3. Remove the waste bottle cover and replace it with the stopper from the
waste tubing set. Take note of the distinct fittings at the end of each tube:
one connects to the Vacuum port, the other to the Waste port on the back
of the washer.
4. To install the optional inline vacuum filter:
1. Cut the tubing that will connect the waste bottle to the Vacuum port
leaving about an inch of tubing extending from the washer,
2. Insert the vacuum filter between the cut ends of the tubing.
5. Attach the tubing from the waste bottle to their respective ports on the back
of the washer:
•Vacuum port to connect the vacuum pump to the waste bottle,
•Waste port to capture waste fluid from the washer.
6. Connect the tubing from the vacuum filtration carrier to the Carrier port on
the back of the washer.
Disconnect the waste tube from the Carrier port when you are not
using the vacuum filtration carrier. An internal valve preserves the
vacuum required to perform standard aspiration.
Install the Liquid Level Alert System (Optional)
The Liquid Level Alert select box has ports, labeled A, B, and C, for the
supply bottles, and Waste.
1. If you have not already done so, connect the waste and fluid supply
bottles as described on the previous pages.
2. Connect the cables from the supply and waste bottles to the select box: