Harvard Bioscience BTX 630B User manual

Safety Stands
USER’S MANUAL
Publicaon 5502-002 Rev 2.0
45-0207 (Model 630B) Cuvee Safety Stand
47-0208 Extended Capacity Flatpack Safety Stand
47-0203 AgilePulse MAX Cuvee Safety Stand
47-0209 AgilePulse MAX Extended Capacity Flatpack Safety Stand
47-0610N AgilePulse PLUS Extended Capacity Flatpack Safety Stand

Thank you for invesng in a Safety Stand.
Since its founding in 1983, the main focus of BTX has been in the area of applying
controlled electric elds for genec engineering applicaons. Because of this,
we quickly established a reputaon as the technological leader in the elds of
electroporaon and electrofusion. Our systems have been installed in many
presgious instutes around the globe where they are used successfully for high
eciency transfecon, transformaon and cell fusion applicaons. We oer a
variety of waveforms, electrodes and chamber opons to provide you with the
best tools to achieve your goals.
We are vested in your success. To that end, the BTX technical support team
constantly tracks published literature for any reference to electroporaon and
electrofusion. We extract the pernent experimental condions and yields
from these papers to help us in our eorts to help you. In addion to tracking
publicaons, we are available to you for support at any me for advice in
experimental design, product recommendaons, troubleshoong, and any other
relevant technical advice.
We thank you again for your investment and we look forward to assisng you in
any way we can.
Finally, please read this manual carefully before operang. If you have any
quesons about the unit or about parcular applicaons, please contact us:
BTX
84 October Hill Road
Holliston, MA 01746 USA
Toll Free: 1-800-272-2775
Internaonal Callers: 508-893-8999
Fax: [phone]
Web: www.btxonline.com
Email: [email protected]
For any customers outside the US or Canada, please call your local BTX dealer
or call us directly.
A Message from BTX
Safety Stand
5502-002 Rev 2.0 • www.btxonline.com

1
Safety Stand
5502-002 Rev 2.0 • www.btxonline.com
Table of Contents
SUBJECT PAGE #
Warranty Informaon 2
General Safety Informaon 3
Introducon 4
Operaon: Geng Started 5
Specicaons 6
Ordering Informaon 7

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Safety Stand
5502-002 Rev 2.0 • www.btxonline.com
Warranty Information
FOR RESEARCH USE ONLY
BTX
84 October Hill Rd
Holliston, MA 01746, USA
Phone: [phone]
Fax: [phone]
Web: www.btxonline.com
Warranty
BTX warranes this Safety Stand for a period of 90 days from
the date of purchase. At its opon, BTX will repair or replace the
unit if it is found to be defecve as to workmanship or materials.
This warranty does not extend to equipment which has been (a)
subjected to misuse, neglect, accident or abuse, (b) repaired or
altered by anyone other than BTX without
BTX express and prior approval, (c) used in violaon of instrucons
furnished by BTX. This warranty extends only to the original
customer purchaser. IN NO EVENT SHALL BTX BE LIABLE FOR
INCIDENTAL OR CONSEQUENTIAL DAMAGES. Some states do not
allow exclusion or limitaon of incidental or consequenal damages
so the above limitaon or exclusion may not apply to you. THERE
ARE NO IMPLIED WARRANTIES OF MERCHANTABILITY, OR FITNESS
FOR A PARTICULAR USE, OR OF ANY OTHER NATURE. Some states
do not allow this limitaon on an implied warranty, so the above
limitaon may not apply to you. Without liming the generality
of the foregoing, BTX shall not be liable for any claims of any kind
whatsoever, as to the equipment delivered or for non-delivery of
equipment, and whether or not based on negligence. Warranty is
void if the instrument is changed in any way from its original factory
design or if repairs are aempted without wrien authorizaon
by BTX. Warranty is void if parts, connecons, or power supplies
not manufactured by BTX are used with the BTX Safety Stand. If a
defect arises within the warranty period, promptly contact BTX, 84
October Hill Road, Holliston, Massachuses, USA 01746-1388 using
our toll free number 1-800-272-2775 (US Only) or 508-893-8999.
(E-mail: [email protected]). Goods will not be accepted for
return unless an RMA (Returned Materials Authorizaon) number
has been issued by our customer service department. The customer
is responsible for shipping charges. Please allow a reasonable
period of me for compleon of repairs, replacement and return.
If the unit is replaced, the replacement unit is covered only for the
remainder of the original warranty period dang from the purchase
of the original device. This warranty gives you specic rights, and
you may also have other rights, which vary from state to state.
Out of Warranty Service
Proceed exactly as for Warranty Service above. If our service
department can assist you by phone or other correspondence,
we will be glad to help at no charge. Repair service will be billed
on the basis of labor and materials. A complete statement of
me spent and materials used will be supplied. Shipment to BTX
should be prepaid. Your bill will include return shipment freight
charges. Disassembly by the user is prohibited. Service should
only be carried out by experienced BTX technicians.
Repair Facilies and Parts
BTX stocks replacement and repair parts. When ordering, please
describe parts as completely as possible, preferably using our part
numbers. If praccal, enclose a sample photo or drawing.
Cauon Noce
The BTX Safety Stand is intended for laboratory use only and can
be used in research and development applicaons. These systems
have been designed to meet the standards for electromagnec
compability (EMC) and safety. This product should not be used
in the presence of a ammable atmosphere such as an anesthec
mixture with air, oxygen, or nitrous oxide.

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Safety Stand
5502-002 Rev 2.0 • www.btxonline.com
Safety Information
Please read the following safety precauons to ensure proper use of
your Safety Stand. If the equipment is used in a manner not specied,
the protecon provided by the equipment may be impaired.
To Prevent Hazard or Injury
Make Proper Connecons
Make sure all connecons are made properly and securely.
Any signal wire connecons to the unit must be no longer than
three meters.
Observe All Terminal Rangs
To avoid re or shock hazard, observe all rangs and markings on
the product or in this manual before using the device.
Avoid Exposed Circuitry
Do not touch any electronic circuitry inside of the product. Do not
insert ngers or try to remove electrode or sample during pulsing
sequence.
Do Not Operate with Suspected Failures
If damage is suspected on or to the product do not operate the
product. Contact qualied service personnel to perform inspecon.
Place Product in Proper Environment
Review the operang manual for guidelines for proper operang
environments. Do not operate in wet/damp condions.
Wear proper eye protecon
during electroporaon
Arcing Can Occur at High Voltages
An unfavorable combinaon of parameters such as high voltage
sengs and a small sample volume with a highly conducve
medium might lead to ashover between the electrodes (ARC) and/
or explosive evaporaon of the medium. Reduce voltage or pulse
length to avoid repeang this condion.
Observe all Warning Labels on Product
Read all labels on product to ensure proper usage.
High Voltage Risk
These BTX Safety Stands are designed to connect to a high voltage
power supply adjustable to 3,000 V. High voltage power supplies
present a serious risk of personal injury if not used in accordance
with design and/or use specicaons, if used in applicaons on
products for which they are not intended or designed, or if they
are used by untrained or unqualied personnel.
• The user must read this manual carefully before the
instruments are placed into operaon.
• Removing the cover will void the warranty.
• Do not connect or disconnect the high voltage cable with
the high voltage enabled.
• To connect or disconnect the cable, turn line power o and
unplug line (mains) cord.
• Do not touch the electrode p while the waveforms are
being applied.
If there are any quesons about the operaon of this equipment,
call BTX Customer service at 1-800-272-2775, or 1-508-893-8999.
Cauon Noce
The BTX Safety Stand is intended for laboratory use only and can
be used in research and development applicaons. These systems
have been designed to meet the standards for electromagnec
compability (EMC) intended for laboratory equipment
applicaons as well as the applicable safety requirements for
electrical equipment for measurement, control, and laboratory
use. The unit itself does not generate waste, but may be used to
treat samples that are hazardous. Please use appropriate PPE and
ensure disposal in accordance with local regulaons and pracces.
This product should not be used in the presence of a ammable
atmosphere such as an anesthec mixture with air, oxygen, or
nitrous oxide.
Cauon Risk
of Electric Shock
Cauon

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Safety Stand
5502-002 Rev 2.0 • www.btxonline.com
The BTX Safety Stand is a safety device for use with BTX disposable cuvees and atpack chambers. Its unique design ensures that no
current can be delivered unless the lid is closed, therefore protecng the user from high voltages. Up to two cuvees can be electroporated
at once, thus decreasing experimentaon mes. Please refer to the Product Compability table below for compable BTX electroporators
and accessories.
Introduction
Product Compability
BTX Safety Stand Item # 45-0207 47-0208 47-0203 47-0209 47-0610N
Compable
BTX Generators
ECM 399
ECM 630
ECM 830
ECM 2001*
Gemini X2
* When used with
F/F adapters,
Item 45-0088,
Sold separately
ECM 399
ECM 630
ECM 830
ECM 2001**
Gemini X2
** When used
with included
F/F adapters,
Item 45-0088
AgilePulse MAX
ECM 2001+
AgilePulse MAX
ECM 2001+
AgilePulse PLUS
Compable
BTX Cuvees
All 1 mm, 2 mm,
and 4 mm gap
BTX cuvees
All 1 mm, 2 mm,
and 4 mm gap
BTX cuvees
All 1 mm, 2 mm,
and 4 mm gap
BTX cuvees
All 1 mm, 2 mm,
and 4 mm gap
BTX cuvees
All 1 mm, 2 mm,
and 4 mm gap
BTX cuvees
Compable
BTX Flatpacks
45-0109
45-0110
45-0109
45-0110
47-0206
45-0109
45-0110
45-0109
45-0110
47-0206
45-0109
45-0110
47-0206
Note: For compability with older models, please contact BTX Technical Support.

5
Safety Stand
5502-002 Rev 2.0 • www.btxonline.com
Warning High Voltage
Make sure the BTX electroporator
is switched o before connuing.
1. Aach the safety stand cables to the output on the rear or
the front panel of the electroporator depending on the BTX
electroporator being used. For AgilePulse MAX, AgilePulse PLUS,
and ECM 2001+, also connect interlock cable to front panel of
electroporator. For ECM 399, ECM 630, ECM 830, and Gemini
electroporators, connect safety stand cables directly to generator
high voltage output ports. For Legacy ECM 2001 connect safety
stand cables to HV output cables using F/F adapters.
2. Using asepc technique, pipee the correct volume of cell
suspension and reagents into the cuvee or atpack chamber.
If using a cuvee or 10 ml atpack chamber (Item 47-0206),
replace the cap.
3. Open the safety stand shield and place the cuvee or atpack
chamber between the metal contacts. Adjust the distance
between the contacts using the black roller. (Note: the
adjustment will remain the same aer the rst me, do not
readjust the roller with every cuvee. However, check contact
periodically to ensure good current ow). Close the safety
stand shield.
4. Switch on the BTX electroporator. Check that all instrument
sengs and connecons are correct. Deliver electroporaon
pulse protocol by pressing the appropriate START or PULSE
buon depending on the BTX electroporator being used.
5. Aer the pulse delivery is complete, remove the cuvee or
atpack chamber from the safety stand. Use a pipee to remove
the pulsed cells. Treat the pulsed cells according to the protocol.
6. Discard the cuvee, pipee and/or atpack chamber in
a biohazard container. Refer to your local instuon’s
environmental health and safety regulaons for proper
disposal pracces.
7. Unplug safety stand from electroporator. Clean plasc lid with
ethanol and deionized water as needed. Please contact Technical
Support for guidance regarding cleaning procedure for safety
stand metal electrode contacts.
Operation: Getting Started

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Safety Stand
5502-002 Rev 2.0 • www.btxonline.com
Standard Capabilies:
Voltage Range 0 to 3000 VDC; 0 to 250 VAC
Frequency 0.2 to 2.0 MHz
Pulse Length/ 10 μs to 10 s
Time Constants Range
Other Specicaons:
Storage Temperature -10 to 70°C
Operang Temperature 5 to 40°C
Intended Use Indoor Use Only
Relave Humidity 20 to 80%
Maximum Altude 2,000 m (6,562 )
Polluon Degree II
Insulaon Category CAT I
Physical Characteriscs:
Overall Dimensions
(45-0207, 47-0203) L x W x D
12.7 x 10.2 x 17.8 cm (5 x 4 x 7 in)
Overall Dimensions
(47-0208, 47-0209) L x W x D
12.7 x 10.2 x 19.0 cm (5 x 4 x 7.5 in)
Weight
1 kg (2.3 lb)
Specifications

7
Safety Stand
5502-002 Rev 2.0 • www.btxonline.com
Ordering Information
Safety Stands
45-0207 Cuvee Safety Stand (Model 630B) 1 Ea
47-0208 Extended Capacity Flatpack Safety Stand 1 Ea
47-0203 AgilePulse MAX Cuvee Safety Stand 1 Ea
47-0209 AgilePulse MAX Extended Capacity Flatpack Safety Stand 1 Ea
47-0610N AgilePulse PLUS Extended Capacity Flatpack Safety Stand 1 Ea
Replacement Parts
45-0213 Replacement Acrylic Safety Stand Cover (for 45-0207 and 47-0203) 1 Ea
45-0215 Metal Replacement Kit (for 45-0207 and 47-0203) 1 Kit
Accessories and Adapters
45-0208 Cuvee Rack (Model 660) 1 Ea
45-0088 F/F adapters, red and black, for 4 mm diameter banana cables and pin ps 1 Kit
45-0217 Banana to banana cables, red and black, 10  length 1 Kit
47-0097 Adapter, AgilePulse PLUS Generator to AgilePulse MAX Stands 1 Ea
Consumables
45-0134 BTX Cuvees Plus, 1 mm gap, 90 µl, Sterile, Gray cap, individually wrapped, with transfer pipees Pkg. of 10
45-0124 BTX Cuvees Plus, 1 mm gap, 90 µl, Sterile, Gray cap, individually wrapped, with transfer pipees Pkg. of 50
45-0140 BTX Cuvees Plus, 1 mm gap, 90 µl, Sterile, Gray cap, bulk wrapped in packages of 100 cuvees per pkg. Pkg. of 100 x 24
45-0135 BTX Cuvees Plus, 2 mm gap, 400 µl, Sterile, Blue cap, individually wrapped, with transfer pipees Pkg. of 10
45-0125 BTX Cuvees Plus, 2 mm gap, 400 µl, Sterile, Blue cap, individually wrapped, with transfer pipees Pkg. of 50
45-0141 BTX Cuvees Plus, 2 mm gap, 90 µl, Sterile, Blue cap, bulk wrapped in packages of 100 cuvees per pkg. Pkg. of 100 x 24
45-0135 BTX Cuvees Plus, 4 mm gap, 800 µl, Sterile, Yellow cap, individually wrapped, with transfer pipees Pkg. of 10
45-0125 BTX Cuvees Plus, 4 mm gap, 800 µl, Sterile, Yellow cap, individually wrapped, with transfer pipees Pkg. of 50
45-0141 BTX Cuvees Plus, 2 mm gap, 800 µl, Sterile, Yellow cap, bulk wrapped in packages of 100 cuvees per pkg. Pkg. of 100 x 24
45-0109 Flatpack Chambers, 1.83 mm gap, 1.5 ml volume Pkg. of 50
45-0110 Flatpack Chambers, 0.56 mm gap, 80 ml volume Pkg. of 50
47-0206 Flatpack Chambers, 4 mm gap, 10 ml volume Pkg. of 10
47-0002 BTXpress Cytoporaon Medium T Low Conducvity Electroporaon Medium, 500 ml bole 1 Ea
45-0801 BTXpress Electroporaon Soluon, 2 ml bole, up to 20 reacons 1 Ea
45-0802 BTXpress Electroporaon Soluon, 5 ml bole, up to 50 reacons 1 Ea
45-0805 BTXpress Electroporaon Soluon, 10 ml bole, up to 100 reacons 1 Ea
45-0803 BTXpress Electroporaon Soluon Kit, 50 reacons in 2 mm gap cuvees 1 Kit
45-0804 BTXpress Electroporaon Soluon Kit, 20 reacons in 4 mm gap cuvees 1 Kit
45-0806 BTXpress Electroporaon Soluon Kit, 100 reacons in 2 mm gap cuvees 1 Kit
45-0807 BTXpress Electroporaon Soluon Kit, 40 reacons in 4 mm gap cuvees 1 Kit

Contact Us
US & Canadian Customers
phone [phone]
fax [phone]
e-mail [email protected]
website www.btxonline.com
International Customers
To locate a distributor
near you, please visit our
website at www.btxonline.com