LaboGene SCANVAC CoolSafe100-9 PROSuperior XS User manual

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Instruction Manual
CoolSafe100-9 PRO Superior XS
CoolSafe 95-15 PRO Superior XL
© Copyright 2012
LaboGene ApS
Industrivej 6-8
Vassingerød
DK-3540 Lynge.
Tel: [phone]; Mobil: [phone];
Fax: [phone]
Mail: [e-mail];
Internet: www.labogene.com ,
CVR-No.: DK32259154

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In case you have any comments about this manual we will appreciate receiving them at the address below.
Warranty and Liability
LaboGene ApS guarantees that the product delivered has been thoroughly tested to ensure that it meets its
published specifications. The warranty included in the conditions of delivery is valid only if the product has
been installed and used in accordance with the instructions supplied by LaboGene Aps..
LaboGene ApS shall in no event be liable for incidental or consequential damages, including without
limitation, loss of profits, loss of income, loss of business opportunities, loss of use, and other related
exposures, caused by incorrect or in appropriate use of the product.
Symbols used in this manual
WARNING Used in case of danger of a serious accident or when
documentation needs to be consulted.
NOTE Used to direct attention to a special item.

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Table of contents
1. Introduction -------------------------------------------------------------------- 4
2. Safety precautions-------------------------------------------------------------- 4
2.1. General information--------------------------------------------------------- 4
2.2. Safety --------------------------------------------------------------------------- 4
3. Installation ---------------------------------------------------------------------- 5
3.1. Installation of CoolSafe ----------------------------------------------------- 5
3.2. Installation of vacuum pump ---------------------------------------------- 5
3.3. Installation of Shelving module-------------------------------------------- 6
4. Functional description --------------------------------------------------------- 6
4.1. Mains----------------------------------------------------------------------------- 6
5.0. Control panel Pro ------------------------------------------------------------- 6-9
5.1. Operation --------------------------------------------------------------------- 9
5.2 Computer control option ---------------------------------------------------- 9
5.3 Preparation -------------------------------------------------------------------- 10
5.4.Pre-Freezing & Freeze Drying----------------------------------------------- 11
5.5 De-Icing--------------------------------------------------------------------------- 11
5.6 Stopping Vacuum pump---------------------------------------------------- 12
6. Alarms and errors------------------------------------------------------------- 12
7. Maintenance-------------------------------------------------------------------- 13
7.1 CoolSafe------------------------------------------------------------------------ 13
7.2. Vacuum pump -------------------------------------------------------------- 13
7.3. Cleaning ---------------------------------------------------------------------- 13
7.4 Checking for Leaks---------------------------------------------------------- 13
8. The Freeze-drying process--------------------------------------------------- 14
8.1 Hints & Tips------------------------------------------------------------------- 15
8.2 Bibliography------------------------------------------------------------------- 15
8.3 Pressure Table---------------------------------------------------------------- 17
9.0 Factory fitted options-------------------------------------------------------- 18
Enclosure: Declaration of conformity

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Introduction
The CoolSafe models XS & XL are complete Fully Automatic Freeze Dryers
designed for research and pilot scale production applications.
The unique chamber design that locates inside the condenser, offers
reproducible pre-freezing of the samples in incremental steps and a short
vapour pathway, without restrictions, giving uniform freeze drying
performance.
The CoolSafe 100-9PRO Superior XS has a 9litre condenser with lowest
temperature of -100 °C and comes complete with 3 shelf chamber that
locates inside the condenser.
The CoolSafe 95-15PRO Superior XL has a 15litre condenser with lowest
temperature of -95 °C and comes complete with a 5 shelf chamber that
locates inside the condenser.
Freeze-drying is often used for sample preparations and for preservation and
storage of biological and pharmaceuticals materials. Freeze-drying is also
ideally suitable for substances that are heat sensitive.
If you are not familiar with the freeze-drying process, please read Section 7,
which outlines the freeze-drying process.
2. Safety precautions
2.1. General information
Please read this entire manual to fully understand the safe and
effective use of this product. Should you have any comments about
this manual we will be pleased to receive them.
2.2. Safety
In case of overload, an automatic safety device stops the freeze dryer.
All CoolSafe models are electrically protected with an over current circuit
breaker.
All CoolSafe models utilize low temperature refrigeration to either -95°C or -
110°C and under no circumstances should the cold surfaces of the
condenser be touched whilst switched on, in operation or in the de-icing
process.
Do not handle, move or service the vacuum pump whilst in operation or if it
is switched Off, until has cooled down to ambient.(see 7.2 p 13 regarding
maintenance & changing of oil)

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It is advisable that Safety glasses and personal protective garments be worn
by all User’s of the equipment. CoolSafe XS & XL Models are fitted with
castors, the front pair being lockable. Once located in the position conducive
to both the safety of the laboratory environment and personnel, it is advised
that the castors be in the locked position.
The safety system is designed according to Safety requirements for electric
equipment, control and laboratory use (EN 61010-1), which assumes that
two separate faults do not occur simultaneously.
3. Installation
3.1. Installation of the CoolSafe
Make sure that the mains supply is according to the specifications on the
type label at the rear panel.
It is important that free air circulation is available at the air inlet and outlet
grills. If the CoolSafe is positioned against a wall, there must be at least 100
mm free space at the rear of the unit.
CoolSafe 100-9 PRO Superior XS & CoolSafe 95-15 PRO Superior XL are both
floor standing units fitted with castors, the front pair being lockable. Before
operating the system insure that the castors are in the locked position.
3.2. Installation of vacuum pump
A two stage vacuum pump (with 2-5 m3/hour capacity or greater) must be connected
to the CoolSafe via the connection on the rear of the CoolSafe in order to allow cryo
pumping.
The vacuum pump can be located on the rear panel of the CoolSafe by means of a
shelf support. This is located by using the thumb screws provided-see below.

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LaboGene recommends the VacuuBrand RZ2.5 rotary vane pump for which a variety
of protection accessories, against aggressive materials are available. If the pump
outlet/exhaust is not discharged via a fume hood, an oil mist filter should always be
connected. See accessories section for recommended rotary vane pump and oil mist
filter.
Check that the mains supply for the pump is according to the specifications
on the type label.
Connect the vacuum hose to the suction side of the pump.
Connect the pump to the vacuum connection pipe on the rear panel of the
CoolSafe.
Check the oil level on the pump and fill it if necessary. The oil level sight glass
should be 1/2 - 3/4 full.
LaboGene recommends that when the pump is running the gas ballast valve is in the
open position.
3.3 Installation of the Shelving Unit.
The shelving assemblies for the CoolSafe 100-9 Pro XS (3 electrically heated shelves)
and the CoolSafe 95-15 Pro XL (5 electrically heated shelves) are positioned inside
the condenser and the appropriate connections, via the cables supplied, are made to
the sockets on the rear of the CoolSafe unit.
Both assemblies can be adjusted to provide the required number of shelves
according to sample loading requirements as indicated.
NB. When loading the shelving units into the condenser wear suitable cryo-
protective gloves!
Samples may be loaded directly on to the shelves or by using the optional trays
(cat.no. 7.001.000264), these are particularly appropriate for “bulk samples”.

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4.00 Functional Description
For an overview refer pictures & layout at section 6.1; page 12
4.1 Mains connection
The mains cable connection is made on the rear of the CoolSafe and the mains
ON/OFF switch on the front panel. When switched on the switch is illuminated
green.
5.0 PRO Controller Type SL12a02 & Operation.
SL12a02 is a microprocessor control system for all CoolSafe Pro models. User inputs
and outputs are performed via a keyboard and visualised on the display unit,
SL01a03..
The operator can control the system in a simple menu-structured way as described
in the following text. Due to the many different ways to configure the CoolSafe Pro,
the following paragraphs are only examples.
The keyboard unit consists of a key pad with the keys 0-9, arrow UP and arrow
RIGHT, and a display with 2x16 characters. Operational parameters are entered or
changed via a series of sequential menus.
Generally, the RIGHT-key is the “Enter” key which then leads to the next menu and
the UP-key is for selecting between different possibilities or is used to reset values or
change their signs.
When in a menu, the red LED is on, except when the user is trying to input an out off
range value, causing the LED to “flash”. The LED is off when in NORMAL mode (when
not in a menu), except when an internal or external error is present, in which case
the LED blinks.
NORMAL display:
In accordance with the selected modes (see later):
The display will show the actual process on the first line, and for example a
temperature related to this on the second line. Browse between the possible
variables related to the actual process with the UP-key. The browser is reset to its
start position when pressing one of the numerical keys.

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MAIN menu:
From the main display, key in RIGHT and SELECT MODE between MANUAL,
AUTOMATIC, DE-ICE, RELEASE and STOP. (Note- that not all modes are present on all
models).Press the UP key the appropriate number of times until the desired menu is
shown then press the RIGHT key, and continue as described for each mode in the
following paragraphs.
MANUAL submenu:
Select between COOLING STOP and COOLING START with the UP-key and proceed
with the RIGHT-key.
(A number of other menus may follow depending on the actual CoolSafe model.)
Leaving the menu (common routine for all modes):
When the last menu is reached, select with the UP-key between END MENU WITH
LEAVE (no changes will be saved), END MENU WITH SAVE (all selections will be saved
for later use) and END MENU WITH SAVE AND START (all selections will be saved and
activated on start-up and all other modes will be automatically cancelled). Complete
the programming sequence by pressing the RIGHT-key.

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AUTOMATIC submenu:
Input PREFREEZE, TIME=XX:XX.
Input the max. pressure, PRESSURE, MAX=XXXX.XXX hpa.
In accordance with the configuration:
Input the first shelf-interval, STEP 1, TIME=XX:XX in hours and minutes.
Input the related STEP 1, SHELFTEMP.=XX in degrees C.
Input the related STEP 1, PRESS.=XXXX.XX hPa (the selected value should be <= 90%
off the earlier selected max-pressure).
These 3 steps are repeated a number of times.
Leave the menu as described under MANUAL submenu.
RELEASE submenu:
Leave the menu as described under MANUAL submenu.
DE-ICE submenu:
Input the DE-ICE, TIME =XX:XX in hour and minutes.
Leave the menu as described under MANUAL submenu.
STOP submenu:
Leave the menu as described under MANUAL submenu
5.1 Operation
The freeze drying process is a balance between the heat absorbed by the sample to
vaporize the ice, and the heat removed by the condenser to convert the water
vapour into ice.
It is important to remember following factors when trying to obtain efficient
utilization of the freeze dryer system.
The efficiency of the freeze drying process depends upon:

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The surface area and the thickness of the sample.
The temperature of the condenser and the vacuum obtained.
The eutectic point (melting temperature) and the concentration of the
sample solution.
5.2 Computer Control Option (option 7.001.800.096)
Once all connections have been made to the CoolSafe and software loaded the
screen display will show as below.
This enables the full programming of all the freeze drying parameters via the
computer/laptop and presentation/saving in Excel™ format.
Programmes may be saved for future use, insuring settings and values are the same
for similar samples.
The ability to change parameters easily makes the optimisation of any freeze drying
cycle
very quick and easy to perform.
From the schematic it can be seen that the “yellow” panel are for setting the mode
of operation, the “green” sections indicate current status of temperatures &
pressures. The tabular sections on the right are for entering your values for Pre
freeze (shelf temperature & time), Freeze Drying (temperature, pressure and time).
If you do not use all the freeze drying steps enter 0.00 for time, keeping the
temperature values the same. You must always enter values in “step 10” and
the CoolSafe will continue to operate until you end the cycle.
If you enter a pressure value the freeze dryer will regulate at the value set
but remember to adjust the value required with the Regulation Valve
Entering a zero value for pressure, no pressure regulation is applied, this can
be used in the secondary drying phase or if no regulation is required.
This protocol also applies when entering values using the PRO controller..