Leica STP6000 User manual

Leica STP6000
Operating Manual · Bedienungsanleitung

Published in January 2008 by:
Herausgegeben Januar 2008 von:
Leica Microsystems CMS GmbH
Ernst-Leitz-Straße
D-35578 Wetzlar (Germany)
Responsible for contents:
Verantwortlich für den Inhalt:
Dr. Jasna Röth
(Marketing CMS, Product Management)
(Marketing CMS, Produktmanagement)
Dietmar Gnass
(R&D Manager)
(R&D – Manager)
In case of questions, please contact the hotline:
Bei Fragen wenden Sie sich bitte an die Hotline: Phone [phone] - 29 42 53
Fax [phone] - 29 22 55
E-Mail: [email protected]

1
Leica STP6000
Operating Manual

4
Copyrights
Copyrights
All rights to this documentation are held by Leica
Microsystems CMS GmbH. Reproduction of text or
illustrations (in whole or in part) by print, photo-
copy, microfilm or other method (including elec-
tronic systems) is not allowed without express
written permission from Leica Microsystems
CMS GmbH.
The instructions contained in the following docu-
mentation reflect state-of-the-art technology. We
have compiled the texts and illustrations as ac-
curately as possible. Nevertheless, no liability of
any kind may be assumed for the accuracy of this
manual’s contents. Still, we are always grateful
for comments and suggestions regarding poten-
tial mistakes within this documentation.
The information in this manual is subject to modi-
fication at any time and without notification.

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Contents
Contents
1. Important Notes about this Manual........ 6
2. Intended Use of the Device...................... 7
3. Safety Notes................................................ 8
3.1 General Safety Notes ................................ 8
3.2 Electrical Safety ......................................... 8
3.3 Specific Safety Instructions
for Handling the Touch screen................. 9
3.4 Disposal ....................................................... 10
4. Setting Up and Connecting ...................... 11
5. The Operating Elements ........................... 12
5.1 The Touch screen....................................... 12
5.2 The Function Keys...................................... 13
5.3 The Focus Dial ............................................ 14
5.4 The Smart Module...................................... 14
6. Operation via Touch screen..................... 15
6.1 Basic Settings of the Microscope........... 15
6.2 Contrast Methods....................................... 17
6.3 Magnification.............................................. 19
6.4 Stage and Focus Controls......................... 20
6.5 Configuration............................................... 23
6.6 Touch screen Settings............................... 25
7. Care of the Device ..................................... 26
7.1 Dust Cover ................................................... 26
7.2 Cleaning ....................................................... 26
8. Index ............................................................ 27
9. Appendix ..................................................... 28
9.1 Key Assignments........................................ 28
9.2 Software Update ........................................ 28
9.3 Pictograms and their Meanings .............. 28
10. EU Declaration of Conformity .................. 30

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1. Important Notes about this Manual
(1.2)
→p. 20
!
*
Numbers in parentheses, such as "(1.2)", corre-
spond to illustrations (in the example, Figure 1,
Item 2).
Numbers with an arrow, e.g. →p. 20, refer to a
specific page of the manual.
Caution!
Special safety instructions within this manual
are indicated with the triangle symbol shown
here, and have a gray background.
Caution! The microscope and accessories can
be damaged when operated incorrectly.
Notes on how to dispose on the microscope,
its components and expendables.
Explanatory note.
Item not contained in all configurations.
Text symbols, pictograms and their meanings:
Caution!
This operating manual is an essential com-
ponent of the device, and must be read care-
fully before the device is assembled, put into
operation or used.
1. Important Notes about this Manual
This operating manual contains important in-
structions and information for the operational
safety and maintenance of the device and its
accessories. It must therefore be kept safely for
future reference.

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2. Intended Use of the Device
2. Intended Use of the Device
The Leica STP6000 external control panel (Smart
Touch Panel), to which this operating manual
belongs, serves to control microscopes and sys-
tems with the greatest possible convenience.
It enables remote control of all microscope func-
tions via touch screen and/or function keys.
The Leica STP6000 conforms to the Council Direc-
tives 73/23/EEC concerning electrical apparatus
and 89/336/EEC concerning electromagnetic com-
patibility for use in an industrial environment.
(Only for Systems e.g. Leica AM TIRF MC, AF6000
LX, LMD6000, ...)
Caution!
The manufacturer assumes no liability for
damage caused by, or any risks arising from,
using the devices for purposes other than
those for which they are intended or not us-
ing them within the specifications of Leica
Microsystems CMS GmbH.
In such cases the declaration of conformity
shall cease to be valid.

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3. Safety Notes
3.1 General Safety Notes
This safety class 2 device is constructed and
tested in accordance with
EN 61010-1:2001,
IEC 61010-1:2001,
safety regulations for electrical measuring, con-
trol, and laboratory devices.
Caution!
In order to maintain this condition and to en-
sure safe operation, the user must follow the
instructions and warnings contained in this
operating manual.
Caution!
The devices and accessories described in
this operating manual have been tested for
safety and potential hazards.
The responsible Leica affiliate or the main
plant in Wetzlar must be consulted whenever
the device is altered, modified or used in con-
junction with non-Leica components that are
outside of the scope of this manual.
Unauthorized alterations to the device or
noncompliant use shall void all rights to any
warranty claims!
3. Safety Notes
100-240 V~
50-60 Hz
100-240 V AC, 26 VA
15-35°C
max. 80% up to 30°C
II
2
3.2 Electrical Safety
General specifications
For indoor use only.
Supply voltage:
Frequency:
Power consumption:
Ambient temperature:
Relative humidity:
Overvoltage category:
Pollution degree:

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3. Safety Notes
3.3 Specific Safety Instructions
for Handling the Touch screen
Caution!
Touch the touch screen using your finger
only.
Never use a pen or other hard, sharp or
pointed objects.
Caution!
Do not use or store the device under a con-
dition where the device will be exposed to
water, organic solution or acid.
Caution!
Do not use the device under direct sunlight.
Caution!
Do not use the touch screen when conden-
sation occurs. The condensation inside of the
touch screen is a natural phenomenon and
should disappear after the touch screen is
warmed up.
Caution!
The device‘s electrical accessory compo-
nents are not protected against liquid. Liquid
can cause electric shock.
Caution!
Protect the device from excessive tempera-
ture fluctuations. Such fluctuations can lead
to the accumulation of condensation, which
can damage the electrical and optical com-
ponents.
Ambient temperature: 15-35°C.

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3. Safety Notes
3.4 Disposal
After the end of the product’s life, please contact
Leica Service or Leica Sales on how to dispose
of it.
Please observe the national laws and ordinances
which, for example, implement and ensure com-
pliance with EU directive WEEE.
n.b.!
Like all electronic instruments, the control
panel, its components and expendables may
not be disposed of as general household
waste!

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4. Setting Up and Connecting
4. Setting Up and Connecting
First, carefully remove all components from the
transportation and packaging materials.
Caution!
Do not yet connect the microscope, the Leica
STP6000 and peripherals to the power supply
at this point!
Caution!
Electrical components must be placed at least
10 cm away from the wall and away from
flammable substances.
Fig. 1 Leica STP6000 connector panel
1On/off switch
2 Power supply connection
3, 4 For service only
5Microscope (USB) connection
• Connect the provided power supply unit to the
power supply connection of the Leica STP6000
(1.2). Tighten the retaining ring to secure the
plug connection.
• Connect the USB terminal of the stand to the
USB connection (1.5).
• After all connections have been made, connect
the power supply unit of the Leica STP6000 to
the main power connection.
• Switch on the microscope as usual.
• After initialization, switch on the Leica STP6000
using the on/off switch (1.1).
12 3 4 5

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5. The Operating Elements
5. The Operating Elements
Fig. 2 Control elements of the Leica STP6000
1Touch screen
2Information key
3, 4 Variable function keys, user-programmable
5Fine focus adjustment
6Coarse focus adjustment
7Movement in Y direction
8Movement in X direction
Microscope functions can be controlled from the
external control panel.
This is done using:
1. The touch screen (2.1)
for adjusting microscope components and
methods, as well as programming the function
keys.
2. The variable function keys (2.3, 2.4),
which can be assigned the most frequently
used functions.
3. The focus dial (2.5, 2.6)
for coarse and fine focusing.
4. The smart module (2.7, 2.8)
for moving the stage.
5.1 The Touch screen
After the device is switched on, the display shows
the current microscope status. The display and
functions that can be operated using the touch
screen depend on the features of the individual
microscope and its accessories.
The meanings of the most important pictograms
are listed in the Appendix →p. 28.
The display shows various levels of operation:
Level 1: Navigation bar
Level 2: Menu bar
Level 3: Control panel field
When you select a pictogram in the navigation
bar (on the left-hand border of the display), the
corresponding menu bar (on the top border of the
display) is shown. The display in the control pan-
el field changes accordingly. For some functions,
an additional information field appears above the
operating keys.
Navigation bar Menu barControl panel
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34 56
7
8

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5. The Operating Elements
The navigation bar allows you to quickly move
between navigation items:
Basic microscope settings
Contrast methods
Magnification
Stage and focus controls
Configuration
Touch screen settings
For the function description, see Chapter 6,
"Operation via Touch screen", on p. 15.
Note:
Before working with the touch screen, we rec-
ommend calibrating it first →p. 25.
5.2 The Function Keys
You can assign the most frequently used func-
tions to the function keys (2.3) and (2.4).
When the Leica STP6000 is shipped from the fac-
tory, these keys have default functions assigned
according to your device configuration. The key
assignment is documented on the information
sheet included with your shipment.
The red function key (2.2) serves as the informa-
tion key. If this key is pressed, the current as-
signment of the function keys (2.3) is displayed
→p. 23.
All other keys can be reassigned. The keys are
programmed using the touch screen ("Micro-
scope configuration" navigation item, "Touch-
Fkeys" menu item →p. 23).

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5. The Operating Elements
Fig. 3 X, Y and Z operation
1Fine focus adjustment
2Coarse focus adjustment
3Movement in Y direction
4Movement in X direction
5Locking screw for smart module
5.3 The Focus Dial
The focus dial for coarse and fine focusing of
the Leica STP6000 is located on the right side
(3.1, 3.2).
5.4 The Smart Module
The smart module is used for moving the stage in
the X and Y directions.
The position of the smart module can be adjusted
individually by unscrewing the locking screw (3.5).
Retighten the locking screw after the adjustment.
12 3
4
5

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6. Operation via Touch screen
6. Operation via Touch screen
6.1 Basic Settings of the Microscope
Status/Illumination menu
After the device is switched on, the status screen,
which displays the current microscope setting,
appears first.
The current contrast method appears in an infor-
mation field.
The information varies according to the micro-
scope configuration. In this example, the trans-
mitted light axis is selected, the transmitted light
shutter is closed (gray) and the current values for
the brightness, aperture and field diaphragm set-
tings are displayed.
The values are changed using the +and - keys.
For the brightness adjustment, you can also
chose between coarse adjustment and fine
adjustment in individual steps by touching the
middle of the adjustment field (the key with the
numerical value).
When you toggle between the transmitted and in-
cident light axis, the operating elements change
accordingly.
In this example, the incident-light axis is selected
and the incident-light shutter open (yellow). The
type of diaphragm (round/rectangular) is also
displayed for the field diaphragm setting.
Use the +and - keys to change the size and shape
of the diaphragm.
Further examples:
Cycling through the intensity levels of the Fluo-
rescence Intensity Manager via the +and - keys
Selection of mirror housing positions

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6. Operation via Touch screen
Selection of transmitted-light filters
Shutter control on inverted stands
Memory-Function menu
Up to 6 user-specific settings can be saved. In
doing so, combinations of contrast method and
objective are stored. One exception is fluores-
cence mode. In this mode, the combination of
fluorescence filter and objective is stored.
To teach in the settings, set the desired combina-
tion, select a key to be assigned, then press the
Set key.
To delete a stored combination, press the corre-
sponding key, e.g. TL-BF – 10x, then the Clear
key.
To retrieve a stored combination, press the cor-
responding key, then press GoTo.
Electrophysiology menu
Additional menus can appear for specific sys-
tems, in this case the Electrophysiology applica-
tion.
The special modules for the Leica DM6000 CFS
(fixed stage) stand can be operated in this menu.
TL-Mirror corresponds to a motorized mirror that
operates the transmitted light detector of the TCS
SP5 (TLD 1ch). The Side key activates the detec-
tor, and the Straight key enables the halogen
lamp.
Infrared Filter activates or deactivates an IR fil-
ter in the transmitted light beam path using the In
and Out keys (720 nm - 850 nm).
One of the three switch positions of the magnifi-
cation changer, 0.35x, 1x or 4x is selected for the
CCD camera under Port-Magn.Changer.
Finally, you can select either the eyepieces or the
camera as the observation port under Tube.

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6. Operation via Touch screen
6.2 Contrast Methods
You can choose from any of the contrast methods
that are available for your microscope configura-
tion.
Example for transmitted-light method
The DIC contrast method is selected. The DIC fine
adjustment is performed using the +and - keys
and the prism Cis selected.
In the case of manual, encoded DIC prisms, the
prism to be selected is indicated and marked with
an exclamation point. Once the prism is selected,
the exclamation point disappears and the prism
is marked in red.
The TL shutter is closed in this example.
Example for incident-light method
The BF contrast method is active and the inci-
dent-light shutter is open. The desired filter cube
can be selected if necessary.
Example for fluorescence method
The FLUO contrast method is active and the BGR
fluo filter cube is selected.
Press FLUO again to choose between:
Fluorescence Intensity Manager (FIM)
(cycling through the intensity levels of the Fluo-
rescence Intensity Manager via the +and - keys)

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6. Operation via Touch screen
Internal Filter Wheel (IFW)
(selecting the desired filter via the color wheels)
Excitation Manager (ExM)
(setting the Excitation Manager via the +and -
keys)
Example for combination method
In the FLUO-DIC combination method, fluores-
cence is selected, the incident-light shutter open
and the fluo cube Aactivated.
To switch to the DIC method, press FLUO-DIC
again. The display will change, letting you select
the prism and perform the DIC fine adjustment.

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6. Operation via Touch screen
6.3 Magnification
The information field on the top edge of the
screen shows the current total magnification at
the specified observation port.
By pressing the
key, you can toggle between the various obser-
vation ports.
Example for upright microscopes:
Example for inverted microscopes:
The objective keys are labeled with the corre-
sponding objective magnification. The objectives
permitted for the selected contrast method are
identified with a white triangle.
Immersion objectives are also marked with drop
symbol.
To switch between objectives, press the corre-
sponding key; the key then changes color.
The desired observation port and the position of
the magnification changer can be selected.

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6. Operation via Touch screen
6.4 Stage and Focus Controls
Focus drive menu
for Leica DM and DMI microscopes
The current Z position is shown in the information
field.
The graphic in the middle of the control panel
field is used for orientation. The current Z po-
sition is marked and shown as a number. If the
focus position and lower stop have been set, this
is also displayed.
Z position
Focus position
set
Lower stop
set
Press the
button to travel directly to the focus position.
Press the
button to reach the lower stop. The Z drive contin-
ues only as long as the button is held depressed.
The process can be interrupted at any time to
prevent a collision of the objective and specimen
in case of an incorrectly set lower stop.
To set or clear the focus position or the lower
stop, select the corresponding position under
Set/Clear, then press the Set or Clear key.
Press to travel to the set or go to the top
stop when using an inverted stand.
The focus position and stops are stored by the
microscope and retained from one session to the
next when power is switched off.
Use Coarse and Fine to switch between coarse
and fine focusing at the stand handwheel or the
focus knob of the Smartmove as applicable.