leroy biotech ELECTROvet EZ User manual

35 Boulevard du Libre Echange – 31650 Saint-Orens-de-Gameville
FRANCE
TEL: [phone]
WEB: www.leroybiotech.com
EMAIL: [email protected]
USER MANUAL V 1.1
ELECTROvet EZ
Z

USER MANUAL
1. INTRODUCTION 3
2. BASIC FEATURES 3
General characteristics 3
General view 4
Symbols and marking 5
Front panel 6
Rear panel 6
Double foot-switch 6
Contact electrode 7
Needle electrode 7
3. GETTING STARTED 8
The unique treatment identifier 8
Start-up 9
The Pulse Evaluation System 10
When does GREEN LED LIGHT ON? 10
When does RED LED LIGHT ON? 10
LCD screen, LED lights& buzzer indications 11
Complete shut-off 11
4. RECOMMENDATIONS FOR USE 12
General recommendations 12
The device 12
The electrodes 12
The environment 13
The contraindications 13
The liability 13
The warranty 14
5. MAINTENANCE 15
Cleaning the box and the foot-switch 15
Cleaning, disinfection and sterilization of the electrodes 15
6. TROUBLESHOOTING 16
7. TECHNICAL SPECIFICATION 17
Protection 17
User interface 17
Standards 17
Disposal and recycling 18
Manufacturer ID 18
8. OUTPUT POWER CURVES 19
History of the treatment 20
9. Connection to an electrocardiogram 22
10.SETTING ELECTRICAL PROTOCOLS 23
General characteristics 23
Recommendations for use 23
Use 23
Technical Characteristics 27
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1. INTRODUCTION
Thank you for choosing the ELECTROvet EZ designed to realize veterinary
electrochemotherapy sessions.
After removing from the box, please check for any transit damage, if damage to the box is found,
please contact LEROY Biotech immediately.
Together with this unit, you will find the following:
-1 double foot-switch ([1 CHARGE] — [2 PULSE])
-1 removable mains lead with earthed connection
-1 electrode
-1 pen
-1 caliper
- 1 USB key containing:
- your invoice
- the user manual of your ELECTROvet EZ
- Terms of Sales of LEROY Biotech
2. BASIC FEATURES
ELECTROvet EZ is a pulse generator. This veterinary device is used for the treatment of tumors
on animals.
General characteristics
-Treatment with electrochemotherapy (applying electrical pulses into a saturated tumor with
cytotoxic agent).
-Standard electrical protocol.
-Control by foot-switch.
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General view
1
ON/OFF switch
8
Foot-switch connector
2
Fuse housing
9
ON LED
3
Electrical plug
10
Charge LED
4
Electrocardiogram connector
11
Active LED
5
USB port
12
Failure LED
6
LCD Display (2 lines)
13
Electrode
7
Electrode connector
14
Double foot-switch
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13
14
1
2
3
4
5
7
8
9 10 11 12
6

Symbols and marking
Symbols
Meaning
Symbols
Meaning
Caution electrode
connector with high
voltage, electric shock
hazard
100-240
V
47-63Hz
Electrical alimentation
Footswitch connector
XXXXX
Serial number
Power indicator
15W
Power
Charge indicator
Time-delay fuses
characteristics
Pulse delivered indicator
IP30
International Protection
Marking 30
Pulse undelivered indicator
Grounded connection
mandatory
Pulse transmission
indicators
CE marking
[email protected]
m
Manufacturer email
address
Refer to this document
GHT-EZ003A
Part number
USB port
Electrocardiogram connector
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Front panel
This comprises indicator lamps, LCD Screen
LED and connectors:
-LCD Screen LED: displays a unique
treatment number as well as information on the
operation of the machine.
-Electrode connector: connection to the
different kind of electrodes.
-Foot-switch connector: connection to the
double foot-switch.
-ON LED: this is blue, lights up when ON/OFF switch of the device is ON (I).
-CHARGE LED: this is orange, lights up after the foot-switch [1 PULSE] have been pressed.
Note: a single "beep" is heard parallel to the press of the foot-switch [1 PULSE].
-ACTIVE LED: this is green, lights up when electrical pulses have been fully transmitted after
pressing the foot-switch [1 CHARGE] then [2 PULSE]. Note: a double "beep" is heard parallel
to the press of the foot-switch.
-FAILURE LED: this is red, lights up when the electrical pulses were not able to be delivered
correctly, after pressing the foot-switch [1 CHARGE] then [2 PULSE]. Note that a long "beep"
is heard parallel to the press of the foot-switch.
Rear panel
-ON/OFF switch: Position I: ON.
-Fuse housing: contains the fuses designed
to protect the unit.
-Electrical Plug: connection to the power
source by a standard plug.
-Electrocardiogram connector: connection
to an electrocardiogram. Only when needed.
-USB port: contains the USB key on which
all your treatments are automatically
recorded.
Double foot-switch
-The double foot-switch sends the electrical
pulses. One should successively press the
left pedal [1 CHARGE] then the right pedal [2
PULSE] for sending pulses.
-The left foot-switch [1 CHARGE] allows for
preparation of the device to send pulses
(loading capacitors).
-The right foot-switch [2 PULSE] allows to
send pulses, once the orange indicator lamps
is on.
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Contact electrode
This electrode allows the treatment of surface
lesions. It is composed of conductive material
and is applied by placing the part
perpendicular to the handle on the lesion.
Connected to the ELECTROvet EZ, it
delivers 8 pulses (1300 V) of 100μsec each 2
msec. A double orthogonal application
permeabilizes cells on 1 cm3.
Needle electrode
-This electrode allows the treatment of
deeper lesions. It is composed of four
needles of 1.5 cm long.
-Connected to the ELECTROvet EZ, it
delivers 8 pulses (1000 V) of 100μsec each 2
msec. On the contrary of the contact
electrode, only a single application is
needed to permeabilize cells on 0.9 cm3.
-If properly maintained, and not damaged,
these electrodes can be reused up to 20
times (1 use = 1 treatment of a lesion during
a session). Any additional use or use of
damaged and / or contaminated instruments is not allowed.
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3. GETTING STARTED
Before using the electroporator for the first time please take time to read the following pages to
familiarise yourself with the operation of the unit.
We strongly recommend that all users of the electroporator are trained in its operation.
The electroporator is very easy to use. By following this simple operating sequence in
conjunction with the pictures of the electroporator, its panels and its symbols, you will be able to
ensure your session has correctly been done every time.
Use of ELECTROvet EZ is restricted to qualified veterinarians only in a practice.
The unique treatment identifier
ELECTROvet EZ generates at each start (O / I) a unique number consisting of a series of
digits and letters on 5 alphanumeric characters. This number corresponds to the unique
treatment identifier.
Example of unique treatment identifier: "00178".
The unique treatment identifier is displayed on the top line of the LCD display.
It is very important to note this unique treatment identifier in the medical file of the
treated animal because it will allow to associate the information collected during the
session of electrochemotherapy and the treated animal.
The electroporator saves in a text file all the information on the session and waveforms
corresponding to the electrical current you have sent, collected during your
electrochemotherapy session. This file is saved on the USB key connected to the USB port at
the back of your ELECTROvet EZ.
The name of this file is composed of the unique treatment number followed by the extension
".TXT".
Thus, if you want to review the profile of the electrical pulses received by the animal during the
electrochemotherapy session No. F5YU3, you will have to look on the USB key for the file
name: F5YU3.TXT.
For this reason it is very important to note in the animal's file this unique treatment
number.
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Start-up
1. Place the device on a level surface or on one that has a slope of less than 10°.
2. Connect the electrode to the electrode connector. Important: do not use an electrode if it
looks damaged.
3. Connect the foot-switch to the foot-switch connector.
4. Connect the electrical plug into the electrical socket.
5. Turn the electrical switch to the ON position (I). The blue LED lights up. The device is ready to
use.
6. Note down the unique treatment identifier in your medical file of the treated animal.
7. Electrode is applied on the lesion.
8. Press on the left foot-switch [1 CHARGE], to charge the device. The orange LED lights up,
displayed message on LCD screen : «#CHARGING#» and single «#beep#» is heard.
9. Press on the right foot-switch [2 PULSE], to send pulses.
-If the electrical pulses have been fully transmitted, a double "beep" is heard and the green
LED lights up.
-If the electrical pulses have not been fully transmitted, a long “beep” is heard and the red
LED lights up.
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The Pulse Evaluation System
The ELECTROvet EZ has an innovative pulse evaluation system.
Thanks to this system it is no longer necessary to visualize the current curves and the intensity
curves of the delivered pulses to ensure that the pulsed train has been delivered perfectly and
therefore will be effective.
We explain below in detail how this evaluation system of delivered pulses works:
When does GREEN LED LIGHT ON?
*Green LED is always accompanied by a short double sound.
*Green LED lights on ( accompanied by a short double sound) ONLY when voltage (Volt)
and current (Amps) comply with the following requirements:
#
1°. the average measured current of each pulse is between 0,1 Amps and 20 Amps,
AND
2°. the average measured voltage of each pulse is between:
GREEN LIGHT = GOOD DELIVERY OF PULSES
_____________________________________________________________________
When does RED LED LIGHT ON?
In all other cases than those mentioned above, the red indicator lights up accompanied
by a long blast.
RED LIGHT = WRONG DELIVERY OF PULSES
1,200 Volts and 1,300 Volts for contact electrodes
450 Volts and 500 Volts for needle electrodes.
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