Lighthouse ActiveCount100 User manual

ActiveCount100
MICROBIOLOGICAL SAMPLER
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Copyright © 2018 by Lighthouse Worldwide Solutions. All rights reserved. No part of this
document may be reproduced by any means except as permitted in writing by Lighthouse
Worldwide Solutions.
The information contained herein constitutes valuable trade secrets of Lighthouse Worldwide
Solutions. You are not permitted to disclose or allow to be disclosed such information except as
permitted in writing by Lighthouse Worldwide Solutions.
The information contained herein is subject to change without notice. Lighthouse Worldwide
Solutions is not responsible for any damages arising out of your use of LMS applications and
Lighthouse instruments.
ActiveCountTM, ActiveCount100TM, ActiveCount100HTM LMS™, LMS Express™, LMS Pharma™, and
ApexRemote™ are trademarks of Lighthouse Worldwide Solutions.
Microsoft®, Microsoft Windows™, and Excel™ are trademarks of Microsoft Corporation.
Manufactured by:
Lighthouse Worldwide Solutions
1221 Disk Drive
Medford, Oregon 97501
www.golighthouse.com

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Table of Contents
EU CE DECLARATION OF CONFORMITY .......................................................................................................6
Introduction...................................................................................................................................................................7
ActiveCount100 Basics ..............................................................................................................................................7
Safety Warnings ........................................................................................................................................................7
Touch Screen Calibration ..........................................................................................................................................8
Quick look at ActiveCount100 ...................................................................................................................................9
Plug In USB Flash Drive and Charge Battery............................................................................................................10
Get Ready For Sampling ..........................................................................................................................................11
Interpret Results..........................................................................................................................................................12
Microorganism Sampling Principles ........................................................................................................................12
Constant and Periodic Sampling Modes..................................................................................................................13
Continuous Sampling Exercise.................................................................................................................................15
Periodic Sampling Exercise ....................................................................................................................................16
Security: Configuration Lock....................................................................................................................................17
Status LED Indicates Sampling Status......................................................................................................................18
Sampling Status Screens..........................................................................................................................................19
Sampling Run Metadata ..........................................................................................................................................19
Total Sampling Volume................................................................................................................................................20
Select Total Sampling Volume.................................................................................................................................20
Active Volume Applies to Sampling Runs................................................................................................................20
Locations and Users.....................................................................................................................................................21
Assigns Active Location to Sampling Runs...............................................................................................................21
Assigns Active User to Sampling Runs.....................................................................................................................22
Data Logging –Sampling Metadata.............................................................................................................................23
Save Sampling Metadata to USB Flash Drive ..........................................................................................................23
Status Indicators ..........................................................................................................................................................24
Device Status Indicators ..........................................................................................................................................24
Settings ........................................................................................................................................................................26
ActiveCount100 Settings .........................................................................................................................................26
Tripod/Monopod .........................................................................................................................................................27
Vertical and Horizontal Sampling ............................................................................................................................27
Optional Compressed Gas Sampler .............................................................................................................................28
Verify Nominal Flow Before Gas Sampling..............................................................................................................28
Clean and Disinfect ......................................................................................................................................................31
Avoid Cross Contamination .....................................................................................................................................31
External Enclosure...................................................................................................................................................31
Sampling Head & Dust Cover ..................................................................................................................................31
Transport.................................................................................................................................................................31
Warnings .................................................................................................................................................................32
Calibration ...................................................................................................................................................................33
Calibration –Contact Authorized Facility................................................................................................................33
Technical Specifications...............................................................................................................................................34
Benefits ...................................................................................................................................................................34
Features...................................................................................................................................................................34
Package Contents ....................................................................................................................................................35
Conforming Specifications.......................................................................................................................................35

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Additional Help........................................................................................................................................................35
Appendix A: Database Files.........................................................................................................................................36
Databases in Comma-Delimited Files......................................................................................................................36
Location Database...................................................................................................................................................36
User Database .........................................................................................................................................................36
Sampling Run Databases .........................................................................................................................................37
Appendix B: Feller Conversion Table ..........................................................................................................................39
Sampling Head with 300 Holes x 0.6mm.................................................................................................................39
Appendix C: Limited Warranty....................................................................................................................................40
Limitation of Warranties .........................................................................................................................................40
Warranty of Repairs After Initial Two (2) Year Warranty ........................................................................................40

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EU CE DECLARATION OF CONFORMITY
Manufacturer’s Name: Lighthouse Worldwide Solutions, Inc.
Manufacturer’s Address: Lighthouse Worldwide Solutions, Inc.
1221 Disk Drive
Medford, OR 97501 USA
Declares the product:
Product Name: ActiveCount
Model Number(s): ActiveCount100
Conforms to the following Product Specifications:
SAFETY
EN61010-1:2010
Safety Requirements for Electrical Equipment for
Measurement, Control, and Laboratory Use Part 1:
General Requirements IEC 61010-1:2010
CAN/CSA C22.2
No. 1010.1-1992
Safety Requirements for Electrical Equipment for
Measurement, Control and Laboratory Use, Part 1:
General Requirements
EMC
EN61326-1:2006
Electrical Equipment for Measurement, Control and
Laboratory Use EN 61326-1:2006
UL 61010A-1 - UL Standard for Safety Electrical Equipment for Laboratory Use; Part 1: General Requirements
Replaces UL 3101-1
Supplementary information
The product herewith complies with the requirements of the Low Voltage Directive 73/23/EEC amended
by Directive 93/68/EEC and the EMC Directive 89/336/EEC amended by Directive 93/68/EEC and carries
the CE marking accordingly.
Fremont, CA. Oct 31, 2015 Hanford Choy –Director, Engineering

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Introduction
ActiveCount100 Basics
Monitoring air for live microorganisms is essential when manufacturing hygiene-sensitive products in
safe and sterile environments, such as:
Pharmaceutical, hospital, food & beverage
Cleanrooms, biological safety cabinets, isolators
IAQ indoor air quality, museums, libraries
Cosmetics, textiles, agricultural, environment
Additional aseptic environment monitoring applications
Microorganisms have the potential to contaminate industrial products and harm human health.
ActiveCount100 is a high-performance instrument that uses a well-known method, impaction, to
aspirate air through a perforated plate and deposit airborne bacteria onto an agar-coated petri dish.
The petri dish is then removed, incubated, and tested for live microorganisms.
Safety Warnings
ActiveCount100 Safety Warnings
ActiveCount100 should not be used in Biosafety Level 2 or higher environments.
Do not sample flammable or corrosive gases.
ActiveCount100 gas sampler is designed to sample compressed air, CO2or NO2only.
To minimize the chance of electric shock, turn off the instrument and disconnect
power adapter during sterilization.
Do not charge ActiveCount100 with a power supply other than one provided by
Lighthouse Worldwide Solutions.
Do not submerge ActiveCount100 in any liquids.
Do not spray disinfectant solution directly into ActiveCount100 enclosure.
Do not use gasses to disinfect ActiveCount100.

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Touch Screen Calibration
The ActiveCount100H 3.5” color touch screen is calibrated prior to shipping and there should be no need
to recalibrate at any time. Alternatively touch screen calibration may be performed at any time by
following the steps outlined here.
Touch Screen Calibration
1. Power off the ActiveCount100H.
2. While holding your finger on the touch screen; Power on ActiveCount100H and continue to hold
your finger on the touch screen until the “Performing touch screen calibration” screen appears.
3. Follow the prompts to calibrate the touch screen by pressing and releasing on the filled circles
for all four corners.
Performing touch
Screen calibration.
Touch screen to
Continue.
Press & Release
On the filled
circle
Press & Release
On the filled
circle
To repeat calibration,
Restart the unit while
Pressing the screen
Until the calibration
Prompt appears
Touch screen to
Continue.
Press & Release
On the filled
circle
Press & Release
On the filled
circle

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Quick look at ActiveCount100
User-friendly and easy to use
Large 3.5” (8.9 cm) color touchscreen
Airflow 100 liters per min ± 4%
8 programmable target volumes
50 programmable users
400 programmable locations
Programmable delay start
Continuous and periodic sampling modes
Sampling head dust cover
Autoclavable sampling head and dust cover
Aluminum sampling head
Stainless steel sampling head (optional)
Uses standard ISO 24998 90mm petri dishes
Status LED
Allen wrench to adjust petri dish holder
Lithium ion rechargeable battery
Battery life 6 hours, typical sampling
Battery charge time 4 hours
Data logging to USB flash drive
Store sampling metadata on USB flash drive
Supports 3/8” tripod screws
Tripod adapter for 1/4” tripod screws
Calibration reminder
Calibration certificate
Carrying case
Optional compressed gas sampler

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Plug In USB Flash Drive and Charge Battery
Data logging. Insert the included USB flash drive to store sampling run metadata.
Charge battery. Follow the instructions below to charge the ActiveCount100 battery. To achieve a
fully charged battery, please allow four hours.

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Get Ready For Sampling
Follow these steps to prepare Activecount100 for microorganism sampling. When ActiveCount100
is not in use, always cover sampling head with dust cover.

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Interpret Results
Microorganism Sampling Principles
Microorganisms can be found in droplets suspended in air for long periods, and can contaminate
hygiene-sensitive environments. These droplets fall and settle on surfaces, potentially causing
infections in medical facilities and contamination in manufacturing facilities.
MPN (most probable number) is a well established method to estimate the number of viable
microorganisms in a monitored area. To derive MPN, ActiveCount100 uses the principle that
statistically assumes an increasing number of organisms enter the same hole in the sampling
head as the number of organisms per sample rises.
After sampling, incubate the petri dish to determine the number of Colony Forming Units (CFU).
Then, refer to the Feller Conversation Table in Appendix B to find the Most Probable Number
(MPN) of microorganisms.
As an example, if an incubated agar sample contains 5 CFUs, the Feller Conversion Table shows
the MPN is 5.

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Sampling Modes
Constant and Periodic Sampling Modes
ActiveCount100 supports two sampling modes:
Constant –samples continuously for a user-specified volume of air
Periodic –samples periodically for a user-specified time and volume
Sampling Mode
Description
Constant
ActiveCount100 continuously samples until it reaches the desired sample
volume at 100 liters per minute ± 4%.
For the example below, select 1,000 liters of air as the desired volume.
ActiveCount100 performs the following calculation to determine the sampling
period is 10 minutes.
1,000 liters ÷ 100 liters/minute = 10 minutes

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Sampling Mode
Description
Periodic
ActiveCount100 samples periodically for a user-specified number of cycles
and for a user-specified period until it reaches the user-specified volume at
100 liters per minute.
Periodic mode is useful when monitoring a process over longer periods, e.g.,
manufacturing a batch or lot of food, cosmetics or drugs.
In the example below, select ten cycles over two hours and 1,000 liters of air.
ActiveCount100 determines ten one-minute sampling cycles are needed over
two hours.
1,000 liters ÷ 100 liters/minute = 10 minutes
Pause between cycles. ActiveCount100 calculates the number of minutes to
pause between ten one-minute samples over two hours, or 12.22 minutes.

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Continuous Sampling Exercise
Select Constant mode: At the Home screen, press CONFIGURATION button. Select Constant in
Mode menu item, then select total sampling volume. If desired, also select an optional delay
time (in seconds). Press HOME button to return to the Home screen.
Begin sampling: Press START button to begin sampling.
Save sampling metadata: To save metadata for each sampling run, insert the included USB flash
drive into the rear USB port.

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Periodic Sampling Exercise
Select Periodic mode: At the Home screen, press CONFIGURATION button. Select Periodic in
Mode menu item, then select total sampling volume, delay time, total time to sample and the
number of cycles. Press HOME button to return to the Home screen.
Begin sampling: Press START button to begin sampling.
Store sampling metadata: To store metadata for each sampling run, insert the included USB flash
drive into the rear USB port.

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Np 6
Security Screen
Security
OK
Configuration Lock
MYPWD
Edit Password
MYPWD
H
O
New Password
123
456
A B C D
GE F H
789
0_
IJK L
M N O P
Q R S T U
V W YX Z
#
Enable/Disable
Current Password Edit Password
Sample
Delay
Volume
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Config Screen
Mode Periodic
Cycles 5
Security
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Config Password
MYPWD
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Password
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456
A B C D
GE F H
789
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V W YX Z
#
Sample
Delay
Volume
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Config Screen
Mode Periodic
Cycles 5
Lock Icon
Security: Configuration Lock
ActiveCount100 Sampling Modes can be restricted by locking the Configuration Screen. Before
locking the configuration screen; select the sample mode and sample settings to be locked.
Configuration Lock: With the configuration screen locked; users are restricted to the preselected
sample mode (Constant/Periodic/Gas). The user may not select any other sample mode and may
not change any sample parameters. The user may run the selected sample only.
-From the Home screen press Configuration and then press the LOCK icon button. This will
bring up the Security screen.
-Press the Edit Password button (pencil icon) to bring up the New Password screen
-Enter the new password and press the return key to save and return to the Security
screen.
-Slide the Configuration Lock toggle button to the right for ON or to the left for OFF.
-Press OK to save the Security Settings.

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Status LED Indicates Sampling Status
Status LED color and blink rate: The following table describes ActiveCount100 LED status.
Status
Color
Blink
Idle
Off
Off
Sampling
Blue
Solid
Delay/Pause
Green
Solid
Flow Error
Red
Blinking
Writing to USB
White
Solid
Service/Calibration Required
Yellow
Solid
White LED Indicates Writing To USB Flash Drive: Do not remove USB flash drive
and do not power off ActiveCount100 while LED is white and downloads are in
progress.

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Sampling Status Screens
Display Sampling Status Screens: When a sampling run completes, ActiveCount100 displays a dialog
box to indicate (a) success, (b) manual abort by user, or (c) flow error.
(a) Success (b) Manual abort by user (c) Flow error
Sampling Run Metadata
Display Sampling Results: To display metadata results for the most recent sampling run, press
METADATA button.
Copy Metadata to USB Flash Drive: To append metadata for the current sampling run to a CSV
(Comma Separated Value) file, press COPY button. For more information on ActiveCount100 CSV
files, see Appendix A, Data Files.

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Total Sampling Volume
Select Total Sampling Volume
Constant and Periodic sampling volumes: ActiveCount100 supports up to eight programmable
sampling volumes.
20 liters
50 liters
100 liters
200 liters
300 liters
500 liters
700 liters
1000 liters (default)
Select Active Sampling Volume: When you press on the Volume menu item, ActiveCount100
displays eight sampling volumes. ActiveCount100 indicates the active sampling volume with a
dashed line.
Active Volume Applies to Sampling Runs
ActiveCount100 uses active sampling volume for subsequent sampling runs: To select a new
active sampling volume, press the desired volume button.
Modify Sampling Volume Preset Values: To modify a sampling volume value, press and hold
then release a sampling volume button. ActiveCount100 then displays a keypad to enter a new
sampling volume value. The new value then becomes the active sampling volume.