Lighthouse ActiveCount100 User manual

ActiveCount100
MICROBIOLOGICAL SAMPLER
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Operators Manual
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Copyright © 2018 by Lighthouse Worldwide Solutions. All rights reserved. No part of this
document may be reproduced by any means except as permitted in writing by Lighthouse
Worldwide Solutions.
The information contained herein constitutes valuable trade secrets of Lighthouse Worldwide
Solutions. You are not permitted to disclose or allow to be disclosed such information except as
permitted in writing by Lighthouse Worldwide Solutions.
The information contained herein is subject to change without notice. Lighthouse Worldwide
Solutions is not responsible for any damages arising out of your use of LMS applications and
Lighthouse instruments.
ActiveCountTM, ActiveCount100TM, ActiveCount100HTM LMS™, LMS Express™, LMS Pharma™, and
ApexRemote™ are trademarks of Lighthouse Worldwide Solutions.
Microsoft®, Microsoft Windows™, and Excel™ are trademarks of Microsoft Corporation.
Manufactured by:
Lighthouse Worldwide Solutions
1221 Disk Drive
Medford, Oregon 97501
www.golighthouse.com

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Table of Contents
EU CE DECLARATION OF CONFORMITY .......................................................................................................6
Introduction...................................................................................................................................................................7
ActiveCount100 Basics ..............................................................................................................................................7
Safety Warnings ........................................................................................................................................................7
Touch Screen Calibration ..........................................................................................................................................8
Quick look at ActiveCount100 ...................................................................................................................................9
Plug In USB Flash Drive and Charge Battery............................................................................................................10
Get Ready For Sampling ..........................................................................................................................................11
Interpret Results..........................................................................................................................................................12
Microorganism Sampling Principles ........................................................................................................................12
Constant and Periodic Sampling Modes..................................................................................................................13
Continuous Sampling Exercise.................................................................................................................................15
Periodic Sampling Exercise ....................................................................................................................................16
Security: Configuration Lock....................................................................................................................................17
Status LED Indicates Sampling Status......................................................................................................................18
Sampling Status Screens..........................................................................................................................................19
Sampling Run Metadata ..........................................................................................................................................19
Total Sampling Volume................................................................................................................................................20
Select Total Sampling Volume.................................................................................................................................20
Active Volume Applies to Sampling Runs................................................................................................................20
Locations and Users.....................................................................................................................................................21
Assigns Active Location to Sampling Runs...............................................................................................................21
Assigns Active User to Sampling Runs.....................................................................................................................22
Data Logging –Sampling Metadata.............................................................................................................................23
Save Sampling Metadata to USB Flash Drive ..........................................................................................................23
Status Indicators ..........................................................................................................................................................24
Device Status Indicators ..........................................................................................................................................24
Settings ........................................................................................................................................................................26
ActiveCount100 Settings .........................................................................................................................................26
Tripod/Monopod .........................................................................................................................................................27
Vertical and Horizontal Sampling ............................................................................................................................27
Optional Compressed Gas Sampler .............................................................................................................................28
Verify Nominal Flow Before Gas Sampling..............................................................................................................28
Clean and Disinfect ......................................................................................................................................................31
Avoid Cross Contamination .....................................................................................................................................31
External Enclosure...................................................................................................................................................31
Sampling Head & Dust Cover ..................................................................................................................................31
Transport.................................................................................................................................................................31
Warnings .................................................................................................................................................................32
Calibration ...................................................................................................................................................................33
Calibration –Contact Authorized Facility................................................................................................................33
Technical Specifications...............................................................................................................................................34
Benefits ...................................................................................................................................................................34
Features...................................................................................................................................................................34
Package Contents ....................................................................................................................................................35
Conforming Specifications.......................................................................................................................................35

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Additional Help........................................................................................................................................................35
Appendix A: Database Files.........................................................................................................................................36
Databases in Comma-Delimited Files......................................................................................................................36
Location Database...................................................................................................................................................36
User Database .........................................................................................................................................................36
Sampling Run Databases .........................................................................................................................................37
Appendix B: Feller Conversion Table ..........................................................................................................................39
Sampling Head with 300 Holes x 0.6mm.................................................................................................................39
Appendix C: Limited Warranty....................................................................................................................................40
Limitation of Warranties .........................................................................................................................................40
Warranty of Repairs After Initial Two (2) Year Warranty ........................................................................................40

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EU CE DECLARATION OF CONFORMITY
Manufacturer’s Name: Lighthouse Worldwide Solutions, Inc.
Manufacturer’s Address: Lighthouse Worldwide Solutions, Inc.
1221 Disk Drive
Medford, OR 97501 USA
Declares the product:
Product Name: ActiveCount
Model Number(s): ActiveCount100
Conforms to the following Product Specifications:
SAFETY
EN61010-1:2010
Safety Requirements for Electrical Equipment for
Measurement, Control, and Laboratory Use Part 1:
General Requirements IEC 61010-1:2010
CAN/CSA C22.2
No. 1010.1-1992
Safety Requirements for Electrical Equipment for
Measurement, Control and Laboratory Use, Part 1:
General Requirements
EMC
EN61326-1:2006
Electrical Equipment for Measurement, Control and
Laboratory Use EN 61326-1:2006
UL 61010A-1 - UL Standard for Safety Electrical Equipment for Laboratory Use; Part 1: General Requirements
Replaces UL 3101-1
Supplementary information
The product herewith complies with the requirements of the Low Voltage Directive 73/23/EEC amended
by Directive 93/68/EEC and the EMC Directive 89/336/EEC amended by Directive 93/68/EEC and carries
the CE marking accordingly.
Fremont, CA. Oct 31, 2015 Hanford Choy –Director, Engineering

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Introduction
ActiveCount100 Basics
Monitoring air for live microorganisms is essential when manufacturing hygiene-sensitive products in
safe and sterile environments, such as:
Pharmaceutical, hospital, food & beverage
Cleanrooms, biological safety cabinets, isolators
IAQ indoor air quality, museums, libraries
Cosmetics, textiles, agricultural, environment
Additional aseptic environment monitoring applications
Microorganisms have the potential to contaminate industrial products and harm human health.
ActiveCount100 is a high-performance instrument that uses a well-known method, impaction, to
aspirate air through a perforated plate and deposit airborne bacteria onto an agar-coated petri dish.
The petri dish is then removed, incubated, and tested for live microorganisms.
Safety Warnings
ActiveCount100 Safety Warnings
ActiveCount100 should not be used in Biosafety Level 2 or higher environments.
Do not sample flammable or corrosive gases.
ActiveCount100 gas sampler is designed to sample compressed air, CO2or NO2only.
To minimize the chance of electric shock, turn off the instrument and disconnect
power adapter during sterilization.
Do not charge ActiveCount100 with a power supply other than one provided by
Lighthouse Worldwide Solutions.
Do not submerge ActiveCount100 in any liquids.
Do not spray disinfectant solution directly into ActiveCount100 enclosure.
Do not use gasses to disinfect ActiveCount100.

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Touch Screen Calibration
The ActiveCount100H 3.5” color touch screen is calibrated prior to shipping and there should be no need
to recalibrate at any time. Alternatively touch screen calibration may be performed at any time by
following the steps outlined here.
Touch Screen Calibration
1. Power off the ActiveCount100H.
2. While holding your finger on the touch screen; Power on ActiveCount100H and continue to hold
your finger on the touch screen until the “Performing touch screen calibration” screen appears.
3. Follow the prompts to calibrate the touch screen by pressing and releasing on the filled circles
for all four corners.
Performing touch
Screen calibration.
Touch screen to
Continue.
Press & Release
On the filled
circle
Press & Release
On the filled
circle
To repeat calibration,
Restart the unit while
Pressing the screen
Until the calibration
Prompt appears
Touch screen to
Continue.
Press & Release
On the filled
circle
Press & Release
On the filled
circle

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Quick look at ActiveCount100
User-friendly and easy to use
Large 3.5” (8.9 cm) color touchscreen
Airflow 100 liters per min ± 4%
8 programmable target volumes
50 programmable users
400 programmable locations
Programmable delay start
Continuous and periodic sampling modes
Sampling head dust cover
Autoclavable sampling head and dust cover
Aluminum sampling head
Stainless steel sampling head (optional)
Uses standard ISO 24998 90mm petri dishes
Status LED
Allen wrench to adjust petri dish holder
Lithium ion rechargeable battery
Battery life 6 hours, typical sampling
Battery charge time 4 hours
Data logging to USB flash drive
Store sampling metadata on USB flash drive
Supports 3/8” tripod screws
Tripod adapter for 1/4” tripod screws
Calibration reminder
Calibration certificate
Carrying case
Optional compressed gas sampler

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Plug In USB Flash Drive and Charge Battery
Data logging. Insert the included USB flash drive to store sampling run metadata.
Charge battery. Follow the instructions below to charge the ActiveCount100 battery. To achieve a
fully charged battery, please allow four hours.