Molecular Devices AquaMax 2000 User manual

www.moleculardevices.com
AquaMax®2000/4000
Microplate Washer
User Guide
0112-0151 K
June 2014

This document is provided to customers who have purchased Molecular Devices, LLC
(“Molecular Devices”) equipment, software, reagents, and consumables to use in the
operation of such Molecular Devices equipment, software, reagents, and
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or permit others to use such manufacturers’ and/or their product names as
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Molecular Devices makes no warranties or representations as to the fitness of this
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arising therefrom.
For research use only. Not for use in diagnostic procedures.
Product manufactured by Molecular Devices, LLC.
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The trademarks mentioned herein are the property of Molecular Devices, LLC or their respective owners. These
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Molecular Devices, LLC.

0112-0151 K 3
Contents
Contents. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Foreword . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Who This User Guide Is For . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Conventions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Chapter 1: Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Instrument Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Robot and Stacker Compatibility. . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Components . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Touch Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Wash Heads . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Microplate Carriage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Reagent and Waste Bottle Ports . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Back Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Chapter 2: Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Unpacking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
Setting Up the Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
Connecting to a StakMax®Plate Stacker . . . . . . . . . . . . . . . . . . . . . . 27
Chapter 3: Getting Started . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
Starting the Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
Main Tab . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Running Programs. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
Stopping Programs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Shutting Down the Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
Chapter 4: Operation: The Touch Screen Interface . . . . . . . . . . 37
Administrative Mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
Program Tab . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
Program Tab: Administrative Mode . . . . . . . . . . . . . . . . . . . . . . . . 39
Program Settings Dialog. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40

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Program Options . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
Program Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
Program Stacker Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
Adjust Dispense. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
Program Tab: Program Editing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
New Step Selection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
Dispense Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
Aspirate Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
Wash Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
FastWash Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
Shake Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54
Soak Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
Repeat Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
Purge Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
Rinse Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
Standby Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
Prime Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
Managing the Program Library . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
Plates Tab . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70
Plates Tab: Administrative Mode. . . . . . . . . . . . . . . . . . . . . . . . . . . 71
Plate Definition Dialog . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
Managing the Plate Library . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
Options Tab . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
Advanced Options Dialog . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76
Options Tab: Administrative Mode . . . . . . . . . . . . . . . . . . . . . . . . . 76
Shutdown Options Dialog. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
Managing the Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79
Automatic Shutdown . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79
Restricting Functions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80
Screen Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81
Chapter 5: Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
Technical Support . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
Routine Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84
Purging the Instrument. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
Decontaminating the Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . 86
Cleaning the Exterior. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87
Removing and Storing Wash Heads . . . . . . . . . . . . . . . . . . . . . . . . 88
Cleaning Wash Heads . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91

Contents
0112-0151 K 5
Moving an AquaMax®Microplate Washer . . . . . . . . . . . . . . . . . . . . 92
Preparing for Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93
Chapter 6: Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95
Troubleshooting Charts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95
General Instrument Issues. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95
System Fluidics Issues . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 96
System Pressure Issues . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 97
External Communication Issues . . . . . . . . . . . . . . . . . . . . . . . . . . . 98
Dispense Issues. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 99
Aspiration Issues. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100
Wash Issues. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 102
Cell Wash Issues . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 103
Instrument Error Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 103
Chapter 7: Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 107
AquaMax®Microplate Washer. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 107
Appendix A: Microplate Library . . . . . . . . . . . . . . . . . . . . . . . . 111
Appendix B: Cleaning Solutions . . . . . . . . . . . . . . . . . . . . . . . . 113
Appendix C: AquaMax Sterilant . . . . . . . . . . . . . . . . . . . . . . . . 115
Principle of Use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 115
Contents. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 116
Materials Required but Not Provided. . . . . . . . . . . . . . . . . . . . . . 116
Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 116
Preparing the Reagent . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 117
Preparing the Complete Sterilant . . . . . . . . . . . . . . . . . . . . . . . . . 117
Using Reagent . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 117
Warnings, Precautions, and Limitations . . . . . . . . . . . . . . . . . . . . . 118
Sterilant Solution A Concentrate (4X). . . . . . . . . . . . . . . . . . . . . . 118
Sterilant Solution B. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 118
Appendix D: Tips and Applications . . . . . . . . . . . . . . . . . . . . . . 119
Using the Washer for ELISA Based Applications . . . . . . . . . . . . . 119
Using the Washer for Cell Based Applications . . . . . . . . . . . . . . . 119

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Appendix E: Robot Command Interface . . . . . . . . . . . . . . . . . . 121
Instrument Configurations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 121
Hardware Interfaces . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 121
Host Computer Interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 121
Command Format . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 122
Document Conventions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 122
No Error (OK) Responses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 123
Error Responses. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 123
General Commands . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 124
Initialization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 124
Device Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 125
Motion Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 128
Program Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 129
User Adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 131
Program Management Commands . . . . . . . . . . . . . . . . . . . . . . . . . 132
Factory-defined Protocols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 132
Protocol (Program) Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 133
Edit/view Opened Protocol Steps . . . . . . . . . . . . . . . . . . . . . . . . . 136
Function-specific Step Arguments and Return Data . . . . . . . . . . . . 138
Plate Library Access . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 147
StakMax®Robot Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 151
Stacker Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 151
Errors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 153
Error Reporting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 153
Error Recovery. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 154
Instrument Response . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 154
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 155

0112-0151 K 7
Foreword
Who This User Guide Is For
This guide is intended for users of the AquaMax®2000 and AquaMax
4000 Microplate Washers. The user guide contains setup, operation, and
general maintenance information.
Conventions
Within the scope of this user guide, the following typographical
conventions are used:
CAUTION! Indicates an operation that might cause damage to the
instrument, device, or data, if the precautions are not followed.
WARNING! A warning indicates an operation that can cause personal
injury if precautions are not followed.
Note: Emphasizes significant information in a procedure or description.
Tip: Provides useful information or shortcuts, but is not essential to the
completion of a procedure.

AquaMax®2000/4000 Microplate Washer User Guide
8 0112-0151K

0112-0151 K 9
1
Introduction
The AquaMax®Microplate Washers from Molecular Devices can be easily
configured to meet your current application requirements. The modular
design provides affordable upgrade options when your lab requirements
change.
Instrument Features
AquaMax 2000 and AquaMax 4000 Microplate Washers can be
configured to process 96-well and 384-well microplates used in both
biochemical and cell-based assays.
•Wash heads are available in 96-well and 384-well configurations
for both plate wash and cell wash.
•All wash heads are interchangeable. The software automatically
detects which wash head is installed.
•The AquaMax Microplate Washer primes, cleans, and rinses the
wash heads with the touch of a single button.
•The AquaMax Microplate Washer processes all plate wells
simultaneously during dispense, aspirate, and wash operations.
•Whole-well dispensing and aspiration in 384-well plates result in
2 to 4 times faster plate processing than other commercially
available plate washers.
•The AquaMax Microplate Washer supports most microplate
formats through its user-definable plate library. Up to 32 plate
types can be stored in the plate library.
•The AquaMax Microplate Washer is equipped with two
(AquaMax 2000 washer) or four (AquaMax 4000 washer) reagent
ports to connect 2 or 4 different dispense bottles. Waste can be
drained into any waste bottle. Non-toxic waste can be drained
directly into a sink. The AquaMax Microplate Washer supports
4 L or 10 L bottles only.
•All washer operations are controlled through an on-board
touch-screen user interface.
•The program library can store up to 3 factory-defined and 100
user-defined programs. Each program can contain up to 50 steps.

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Robot and Stacker Compatibility
AquaMax Microplate Washers are compatible with Molecular Devices
StakMax®Microplate Handling System. Third party robots can be set up
to control the AquaMax Microplate Washers through a USB command
interface. The USB command interface is detailed in Robot Command
Interface on page 121.
Components
Table 1-1: AquaMax®4000 Components
Item Description
1 4L reagent reservoirs
2 Back panel with USB and StakMax connectors
3 384-well wash head installed
4 96-well wash head
5 Microplate carriage with microplate
6 Touch screen
2
3
4
6
1
5

Introduction
0112-0151 K 11
Touch Screen
Figure 1-1: AquaMax®touch screen
Instrument operation is controlled through a touch-screen user interface.
The touch screen displays instrument status, provides access to
instrument settings and programs, and controls the optional StakMax
Plate Stacker.
Wash Heads
AquaMax wash heads have either 96 or 384 paired probes. The longer
probe of each pair is used for aspiration. The short probe is used for
dispensing. The plate wash heads have straight dispense probes and are
intended for thorough cleaning and aspiration. The cell wash heads have
angled dispense probes to minimize cell loss during washing.
The wash heads are interchangeable and can be removed and
re-attached using a simple lever mechanism.

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Table 1-2: 96-plate wash head lock
Item Description
1 Close
2 Open
1 2
Note: A mismatched wash head and microplate will cause a motor
pinch error when the wash head tries to descend toward the wells. A
mismatch should not damage the wash head.

Introduction
0112-0151 K 13
Each wash head has a clear plastic guard to protect the probes from
damage, and to prevent spills, sprays, and injury to users.
Table 1-3: 384-plate wash head and guard
Item Description
1 Plastic guard
2 Aspiration and dispensing probes on underside of wash head
12

AquaMax®2000/4000 Microplate Washer User Guide
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Microplate Carriage
The microplate carriage is located on the right side of the washer. The
plate carriage slides in and out of the washing chamber for loading and
unloading.
Table 1-4: Plate carriage
Item Description
1 Plate carriage, in eject position
2 384-well microplate
3 384 wash head
3
2
1
CAUTION! Do not obstruct the movement of the plate carriage during
operation. Obstruction can damage the instrument.

Introduction
0112-0151 K 15
Supported Microplates
AquaMax Microplate Washers accommodate rigid SBS-standard
polystyrene and polypropylene microplates with dimensions 128.2 mm
(L) × 86.0 mm (W), and heights ranging from 13.5 mm to 15.5 mm,
including:
•96-well and 384-well standard formats (U-bottom, V-bottom, or
Flat)
•96-well half area
•96-well and 384-well small volume
The AquaMax Microplate Washer also supports magnetic bead washing
using the Dexter Magnetic Technologies LifeSep magnetic bead-
separator adapter plates. For more details, refer to the Dexter Magnetic
Technologies website: http://www.dextermag.com/lifesep.aspx.
The plate library includes eight commonly used microplates (4 each of
96-well and 384-well):
•Corning/Costar: 96 and 384
•BD/Falcon: 96 and 384
•Greiner: 96 and 384
•Nunc/Nalgene: 96 and 384
Detailed manufacturer numbers and dimensions for these default plate
types are listed in Microplate Library on page 111.
Note: Some microplates with conical or tapered wells and some small
volume microplates are not compatible with bottom wash and aspirate
operations.
Note: 96-well “strip plates” fall outside SBS guidelines, and
compatibility of these plates with the AquaMax Microplate Washer
varies across brands. Plates with strips that are held firmly in a more
rigid frame and have smaller tabs are generally more washer-
compatible. Plates with strips that have large tabs and are held loosely
in a flexible frame, such as Nunc strip plates with C8 configuration,
should be avoided. If you are unsure whether a plate is compatible,
consult Technical Support or your sales representative before
proceeding.

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The plate library can store up to 32 plate types. For information about
adding a plate type, see Managing the Plate Library on page 73.
Reagent and Waste Bottle Ports
The source channels and waste connections are located on the left side
of the instrument. The AquaMax 2000 Microplate Washer has two
source channels, while the AquaMax 4000 Microplate Washer has four.
Each source channel can be connected to a reagent bottle, and is
composed of a fluid input, a pressure output, and an optional level
sensor connector. Waste can be drained into any waste bottle. A waste
sensor can be installed to detect a full waste bottle. Non-toxic waste can
be drained directly into a sink.
The AquaMax Microplate Washer supports 4 L or 10 L bottles only.
Table 1-5: Reagent ports for AquaMax®4000 Microplate Washer.
Item Description
1 Waste full sensor input
2 Waste fluid output
3 Source channels (green, red, blue, and yellow)*
4 Source bottle pressure outputs
5 Source bottle fluid inputs
6 Source bottle empty sensor inputs
1
2
6
5
4
3

Introduction
0112-0151 K 17
*The AquaMax 2000 Microplate Washer has only green and red inlets.
Back Panel
The power switch, power cord receptacle, communication ports, and
instrument information, are located on the back panel of the instrument.
The power cord receptacle and power switch are located on the right
side of the instrument.
Labels and text next to the power switch provide important information
about the AquaMax Microplate Washer, such as line voltage rating and
serial number. Use the model/serial number on this label when
contacting Molecular Devices for technical support issues.
Two communication ports are located on the left side of the back panel.
Table 1-6: Back panel, right side
Item Description
1 Power switch
2 Power cord receptacle
3 Serial number
1
2
3

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18 0112-0151K
The USB Type B port is designated for external robotic control. The
StakMax communication port is dedicated to control StakMax stacker
operations. The StakMax control cable is available with the StakMax
integration kit (Part Number: 0310-5454).
Table 1-7: AquaMax®Microplate Washer USB and StakMax®connectors
Item Description
1 USB port
2 StakMax®connector
12

0112-0151 K 19
2
Installation
WARNING! Shock Hazard. To avoid electrical shock, always make sure
the power switch is in the OFF position, and the power cord is
removed, before installing or moving the instrument.
WARNING! Risk of fire, explosion, or shock. Do not operate the
instrument in an environment where potentially damaging liquids or
gases are present.
CAUTION! Do not operate the instrument in an environment with a
temperature below 15°C.
CAUTION! Do not touch or loosen any screws or parts unless specifically
instructed by Molecular Devices. Doing so can cause misalignment and
voids the instrument warranty.

AquaMax®2000/4000 Microplate Washer User Guide
20 0112-0151K
Unpacking
The AquaMax®Microplate Washers are shipped in two specially designed
containers.
•The base unit box contains the washer unit and accessories
(manual, power cord, waste tube, and tubing label kit):
AquaMax 2000 Microplate Washer (Part Number: 0310-
5363)
AquaMax 4000 Microplate Washer (Part Number: 0310-
5227)
The tubing label kit contains 4×3 pieces of colored slip-on
plastic spiral wraps, one group of each color, for labeling
bottle connections (liquid, pressure, level sensor).
•The wash head box contains up to four wash heads, each in a
clamshell container:
96-Well Microplate Wash Head (Part Number: 0310-5216T)
96-Well Cell Wash Head (Part Number: 0310-5217T)
384-Well Microplate Wash Head (Part Number: 0310-5219T)
384-Well Cell Wash Head (Part Number: 0310-5218T)
The standard AquaMax Microplate Washer unit does not include any
bottles. If you have purchased bottles separately, check to make sure that
all bottles and tubing are present. Each reservoir bottle should contain a
cap, fluid tubing, and air tubing. The waste bottle should contain its own
waste tubing.
•Reagent and waste bottles:
4-Liter Reservoir without Level Sensor (Part Number:
5000801)
4-Liter Reservoir with Level Sensor (Part Number: 5000802)
10-Liter Reservoir (Part Number: 9000-0299)
10-Liter Waste Reservoir (Part Number: 9000-0296)
The AquaMax Microplate Washer supports 4 L or 10 L bottles only.
Keep all boxes and packing materials, including the clamshell containers
for the wash heads. Use the original packing materials for long-term
storage of the instrument. Original packing material must be used for
shipping if the unit is returned for repair.
WARNING! Each AquaMax Microplate Washer weighs approximately
41 lbs (18.5 kg) and should be lifted with care.