NEW BRUNSWICK SCIENTIFIC Innova Series User manual

Ultra-Low Temperature
Freezers
innova®Range
(with vacuum insulation panel technology)
MANUAL No. M1288-0070
Revision E
October 29, 2007
NEW BRUNSWICK SCIENTIFIC CO., INC.
BOX 4005 •44 TALMADGE ROAD •EDISON, NJ 08818-4005
Telephone: 1-732-287-1200 •1-800-631-5417
Fax: 732-287-4222 •Telex: 4753012 NBSCO

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M1288-0070 U360 Ultra-Low Temperature Freezer User’s Manual

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New Brunswick Scientific User’s Guide
INTERNATIONAL OFFICES:
BELGIUM
New Brunswick Scientific NV-SA
Stationsstraat 180/4
3110 Rotselaar
België/Belgique
Tel: 32 (0)16 56 28 31
Fax: 32 (0)16 57 27 53
GERMANY
New Brunswick Scientific GmbH
In Der Au 14
D-72622 Nürtingen
Deutschland
Tel: 49 (0)7022 932490
Fax: 49 (0)7022 32486
CHINA
New Brunswick Scientific Co., Inc.
A903, 904 Yin Hai Building
No. 250, Cao Xi Road
Shanghai 200235, P.R. China
Tel: 86 21 6484 5955 or 5966
Fax: 86 21 6484 5933
THE NETHERLANDS
New Brunswick Scientific BV
Kerkenbos 1101, 6546 BC Nijmegen
P.O Box 6826, 6503 GH Nijmegen
Nederland
Tel: 31 (0)24 3717 600
Fax: 31 (0)24 3717 640
FRANCE
New Brunswick Scientific SARL
3, rue des Deux-Boules
75001 Paris
France
Tel: 33 (0)1 4026 2246
Fax: 33 (0)1 4026 5423
UNITED KINGDOM
New Brunswick Scientific (UK) Ltd.
17 Alban Park
St. Albans, Herts. AL4 0JJ
United Kingdom
Tel: 44 (0)1727 853855 or 0800 581331
Fax: 44 (0)1727 835666
Web: www.nbsuk.co.uk

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M1288-0070 U360 Ultra-Low Temperature Freezer User’s Manual
CAUTION!
This equipment must be operated as described in this manual.
If operational guidelines are not followed, equipment damage and
personal injury can occur.
Please read the entire User’s Guide before attempting to use this unit.

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New Brunswick Scientific User’s Guide
HEALTH AND SAFETY AT WORK ACT 1974
(FOR THE UNITED KINGDOM)
New Brunswick Scientific, as manufacturers and suppliers of laboratory equipment, are
obliged under the terms of the above Act to provide our users with instructions on the safe
installation, operation and maintenance of our equipment.
Our equipment is designed to acceptable standards and does not entail any hazard if used, as
advised in the attached instructions.
The following safety precautions should be observed by all personnel using this equipment:
1. Read and understand this manual. If in doubt, contact one of the New
Brunswick Scientific Companies listed.
2. Do not remove any covers. There are no operable controls other than those
referred to in this manual. There are voltages in excess of 41.5 volts AC
behind the covers.
3. Use freezer gloves at all times when loading or unloading the equipment.
The temperature of operation is such that direct contact with the cold
contents or inside the equipment can burn unprotected skin.
4. Observe good housekeeping practices, at all times keeping the equipment
and the adjacent areas clean, dry and uncluttered.
5. Should any malfunctions occur or be suspected, immediately call a qualified
service engineer to investigate.

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M1288-0070 U360 Ultra-Low Temperature Freezer User’s Manual
Copyright Notice
New Brunswick Scientific Company, Inc.
Box 4005 44 Talmadge Road
Edison, New Jersey 08818-4005 USA
©Copyright 2006 New Brunswick Scientific Co., Inc.
All Rights Reserved.
Reproduction, adaptation, or translation without prior written permission
from New Brunswick Scientific is prohibited.
Disclaimer Notice
New Brunswick Scientific Co., Inc. reserves the right to change
information in this document without notice. Updates to information in
this document reflect our commitment to continuing product
development and improvement.
Manual Conventions
#NOTE:
Notes contain essential information that deserves
special attention.
CAUTION!
Caution messages appear before procedures which, if
caution is not observed, could result in damage to the
equipment.
WARNING!
Warning messages alert you to specific procedures or
practices which, if not followed correctly, could result in
serious personal injury.
Bold Text in boldface type emphasizes key words or
phrases.

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New Brunswick Scientific User’s Guide
New Brunswick Scientific, hereby declares that the product(s) listed below conform to the
European Union directives and standards identified in this declaration.
Product(s) Innova U101 Innova U725
Innova U360 Innova C585
Innova U535 Innova C760
EU Directive(s) CE Marked (93/68/EEC)
Low Voltage (73/23/EEC Amendment 93/68/EEC)
Electromagnetic Compatibility EMC (2004/108/EC)
Conducted/Radiated Emissions (FCC Part 15 Class B)
Standard(s) Low Voltage EN61010-1 First Edition
EMC EN61326 : 1997 A1, A2
The conformity assessment documents are held at the following facility:
NBS Cryo Research Ltd.
Woodrolfe Road
Tollesbury, Essex
CM9-8SE
United Kingdom
United Kingdom
08/21/07
Executive Design Engineer Date
DECLARATION OF CONFORMITY

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M1288-0070 U360 Ultra-Low Temperature Freezer User’s Manual
Every instrument manufactured by New Brunswick
Scientific Co., Inc. is warranted to be free from defects in material
and workmanship. In the USA, this apparatus, with the exception of
lamps (where supplied), is warranted for 2 years (including labor) against
faulty components and assembly; and our obligation under this warranty is
limited to repairing or replacing the instrument or part thereof which shall,
within 2 years after date of shipment, prove to be defective after our
examination. Component parts are warranted for 3 additional years
(excluding labor). Vacuum Insulation Panels (where present) are warranted
for a total of 12 years. This warranty does not extend to any NBS products
which have been subjected to misuse, neglect, accident or improper
installation or application; nor shall it extend to products which have
been repaired or altered outside the NBS factory or its authorized
service centers without prior authorization from the
New Brunswick Scientific Co., Inc.
ÆÆÆ
Outside the USA, contact your NBS
representative for warranty details.
W A R R A N T Y

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TABLE OF CONTENTS
1INSPECTION & UNPACKING OF EQUIPMENT ....................................................11
1.1 INSPECTION OF BOXES/PACKAGING............................................................................11
1.2 PACKING LIST VERIFICATION .....................................................................................11
2INTRODUCTION/OVERVIEW....................................................................................12
2.1 DESCRIPTION OF EQUIPMENT......................................................................................12
2.1.1 Freezer Handles.................................................................................................14
2.1.2 Voltage Stabilizer...............................................................................................14
3INSTALLATION & SETUP...........................................................................................15
3.1 SETUP GUIDE ..............................................................................................................15
3.2 PHYSICAL LOCATION ..................................................................................................15
3.3 SHELF INSTALLATION .................................................................................................16
3.4 CONNECTING TO THE POWER SUPPLY.........................................................................16
3.4.1 Installing the Power Cord..................................................................................17
3.4.2 Power Switch Operation....................................................................................17
3.5 ALARM ACTIVATION &ALARM BATTERY CHARGING................................................18
3.6 REMOTE ALARM SOCKET............................................................................................18
3.7 VACUUM EFFECT........................................................................................................19
4OPERATION ...................................................................................................................20
4.1 OPERATING FEATURES................................................................................................20
4.2 OPERATIONAL CONTROLS...........................................................................................20
4.3 MENU SYSTEM............................................................................................................21
4.3.1 Setting Operating Temperature & Alarm Setpoints...........................................22
4.3.2 Checking Temperature Settings.........................................................................22
4.3.3 Setting Time & Date...........................................................................................23
4.4 ALARM FUNCTIONS &SYSTEM SENSORS....................................................................23
4.4.1 Modifying Alarm Functions...............................................................................24
4.4.2 Temperature Alarm Delay .................................................................................25
4.4.3 Door Open Alarm Delay....................................................................................25
4.4.4 Audible Alarm Mute...........................................................................................25
4.4.5 Alarm Socket Delay............................................................................................25
4.4.6 Alarm Mute & Acknowledgment........................................................................26
4.4.7 Setpoint & Security Control...............................................................................27
4.5 AUDIBLE ALARM BATTERY BACKUP..........................................................................28
4.6 ALARM MONITORING SOCKET....................................................................................29
4.7 DATA STORAGE AND VIEWING ...................................................................................30
4.7.1 Alarm Log ..........................................................................................................31
4.7.2 Cabinet & Ambient Temperature Graph ...........................................................31
4.7.3 1st & 2nd Stage Cycling & Condenser Temp Graphs .........................................32
4.8 DIAGNOSTICS..............................................................................................................33
4.9 PS2 SERVICE DATA PORTS .........................................................................................34

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M1288-0070 U360 Ultra-Low Temperature Freezer User’s Manual
4.10 REMOVAL &REINSTALLATION...................................................................................34
4.11 GENERAL FUNCTIONAL GUIDELINES ..........................................................................34
4.11.1 Safety Alarms.....................................................................................................34
4.11.2 Power Failure....................................................................................................35
4.11.3 Inner Doors or Lids ...........................................................................................35
4.12 VOLTAGE STABILIZER.................................................................................................35
4.12.1 Voltage Stabilizer Installation...........................................................................35
4.12.2 Voltage Stabilizer Operation.............................................................................36
4.12.3 Voltage Stabilizer Specifications.......................................................................36
5MAINTENANCE.............................................................................................................37
5.1 CLEANING...................................................................................................................37
5.1.1 Painted Surfaces ................................................................................................37
5.1.2 Panels & Shelves................................................................................................37
5.1.3 Air Intake Grille & Filter...................................................................................37
5.1.4 Vacuum Release Vent Tube................................................................................38
5.1.5 Door/Lid Seal.....................................................................................................38
5.1.6 Lubrication.........................................................................................................39
5.2 DEFROSTING ...............................................................................................................39
5.3 ELECTRICAL COMPONENTS.........................................................................................39
5.3.1 Audible Alarm....................................................................................................39
5.3.2 Battery Replacement..........................................................................................40
5.3.3 Fuses..................................................................................................................40
6TROUBLESHOOTING..................................................................................................41
6.1 ERRORS,ALARMS &MESSAGING ..............................................................................41
6.2 OTHER SYMPTOMS......................................................................................................42
7OPTIONS & ACCESSORIES........................................................................................43
7.1 ACCESSORIES..............................................................................................................43
7.1.1 CO2& LN2Back-Up Systems ............................................................................43
7.1.2 Racking Systems.................................................................................................43
7.1.3 Chart Recorder ..................................................................................................44
7.1.4 Ethernet/RS-485 Data & Computer Interface ...................................................44
8SPARE PARTS ................................................................................................................45
9SPECIFICATIONS..........................................................................................................46
9.1 UPRIGHT FREEZERS ....................................................................................................46
9.2 CHEST FREEZERS ........................................................................................................47
10 DRAWINGS.................................................................................................................48
10.1 INDEX OF DRAWINGS ..................................................................................................50
10.2 INDEX OF TABLES .......................................................................................................50
11 INDEX...........................................................................................................................51

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1.1 Inspection of Boxes/Packaging
After you have received your order from New Brunswick Scientific, inspect the
boxes/packaging carefully for any damage that may have occurred during shipping.
Report any damage immediately to the carrier and to your local NBS Sales Order
Department.
1.2 Packing List Verification
Unpack your order, saving the packing materials for possible future use. Also be sure
to save the User Manual, for instruction and reference.
Verify against your NBS packing list that you have received the correct materials, and
that nothing is missing.
If any part of your order was damaged in transit, is missing, or fails to operate, please
fill out Customer Satisfaction Form 6300 and return it by fax or mail. You can also
call the New Brunswick Scientific Service Department or your local NBS
representative.
CAUTION!
Inspect the freezer cabinet panels for punctures or other damage that
could compromise the integrity of the freezer.
Any unauthorized punctures or other damage deliberately made to
the cabinet walls will invalidate the warranty.

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M1288-0070 U360 Ultra-Low Temperature Freezer User’s Manual
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This manual provides the user with the necessary information for installation and operation of
the Innova®ultra-low temperature freezer line utlizing vacuum insulation panel technology. It
also provides some preliminary user maintenance information.
2.1 Description of Equipment
The New Brunswick Scientific Innova range of vacuum insulation panel freezers is
designed to provide precise, ultra-low temperature environments for scientific and
medical use. They are designed to provide optimal ultra-low temperature sample
storage from -50oC to -86oC
CAUTION!
Vacuum insulation panel technology is used in the construction of
these freezers. The panels are mounted in the cavity against the steel
outer wall of the freezer. Any drilling or puncture of the outer wall could
release the vacuum from the panel, possibly impairing freezer
performance.
There are two types of Innova freezer: Upright (see Figure 1) and Chest models. This
manual covers all versions of the Innova freezer models:
Table 1: Innova Freezer Models
Upright Freezer Model Capacity
U101 Not Yet Available
U360 12.7 cubic feet (360 liters)
U535 Not Yet Available
U725 Not Yet Available
Chest Freezer Model Capacity
C585 Not Yet Available
C760 Not Yet Available
The freezers are manufactured using high quality steel and electronics for long life.
The cabinets are insulated with a combination of foamed-in-place polyurethane foam
and vacuum insulation panels. This combination ensures the best insulation properties
while maintaining an extra large internal capacity for the footprint of the freezer.
The Innova freezers have many features designed to provide ease of use and maintanence,
security and reliability to your ultra-low temperature storage needs. They are built to
stringent regulatory requirements for safety and environmental friendliness and
disposability, and they are CE registered.

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New Brunswick Scientific User’s Guide
•A large, ergonomically located, backlit LCD display provides all operational
information about the freezer including temperature and setpoint info, on-screen
messaging, programmable alarms, data graphing and even help information.
•Upright models utilize gasketed and insulated inner doors to reduce cold air loss
and recovery times, reduce internal temperature fluctuations and conserve energy.
Chest models utilize insulated covers to reduce internal temperature fluctuations
and conserve energy.
•The freezers utilize vacuum insulation panel technology, reducing the cabinet wall
thickness while maintaining superb insulation characteristics to provide the largest
internal volume per floor space used.
•The freezer’s compressor housing is specially designed to reduce running noise
levels.
•The freezers are supplied with a heated vacuum release port that not only provides
rapid re-opening of the outer door, but also provides a plunger to maintain an open
port.
•All interior panels and shelves are made of high grade corrosion-resistant stainless
steel, making them durable and easy to keep clean and sterilize.
•The freezers are totally free of CFCs (Chlorofluorocarbons) and HCFCs
(Hydrochlorofluorocarbons). They use HFCs (Hydrofluorocarbons) as refrigerants.
•All models are supplied on heavy duty castors for easy installation and relocation
(the model U101 has rear rollers and adjustable front legs)
•The freezers are provided with two access ports for the addition of back-up systems
or sensors that can provide cooling protection for your samples in the case of a
power outage or other system failure.
•The freezers are provided with an external alarm contact that can be used to
connect to an external monitoring device or system.
•The freezers can be fitted with an optional 7-day circular chart recorder to provide
independent temperature recording.
•All models have lockable handles, with an option for a padlock adaptor, to provide
sample security. The microprocessor-controlled system can also be protected from
inadvertent programming changes through the use of passwords and user IDs.

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M1288-0070 U360 Ultra-Low Temperature Freezer User’s Manual
Figure 1: Model U360 Upright Freezer—Side & Front Views
2.1.1 Freezer Handles
The freezers are supplied with lockable handles. The U101, C585 and C760 models
are fitted with quarter turn key locks. The U360, U535 and U725 upright freezer
handles are fitted with push-barrel locks (push in & turn to lock, turn key to unlock).
An optional padlock adaptor can provide extra security by allowing the addition of a
customer-supplied padlock to secure the freezer handle.
#NOTE:
NEVER SLAM ANY FREEZER’S DOOR/LID, particularly with the handle in
the closed position. The door handles have a cam action to pull the
door/lid closed. When closing the outer door/lid, ensure that the cam is
engaged for correct operation. The initial vacuum inside the cabinet may
cause the door to appear closed, but when the vacuum releases, the door
may open. Always ensure the handle is properly engaged.
2.1.2 Voltage Stabilizer
Certain freezer models have the option of being fitted with an automatic voltage
stabilizer. See Section 4.12 for details.

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WARNING!
DO NOT attempt to lift any freezer by hand. Preferred lifting for loading
and unloading is by hydraulic lift.
CAUTION!
Maintenance, adjustment and repair work should be carried out only by
QUALIFIED, EXPERIENCED personnel who have been AUTHORIZED to
undertake such work by New Brunswick Scientific or its authorized
agents.
Failure to use authorized service agents may invalidate the warranty.
3.1 Setup Guide
The following is a quick-start guide to setting up the freezer. More detailed
information is presented in subsequent sections to clarify the process.
1. Unpack the freezer and remove it from its packing pallet.
2. Place the freezer in its designated location.
3. Remove the contents from the inside of the freezer.
4. Unwrap and install the shelves (if provided).
5. Install the power cord and lock it in place.
6. Install the keys in the door lock and the power switch cover lock.
7. Plug the unit into the power supply and turn the unit on.
8. Set the time, date and set temperatures (see Section 4.3.1).
9. Wait until the unit has reached its setpoint temperature before placing anything
inside the freezer.
3.2 Physical Location
All freezers are mounted on castors for ease of movement. Upright freezers have feet
that provide both a leveling feature and a locking feature to stop the freezer from
rolling once it is in place.
Chest freezers have locking front castors; these do not provide leveling adjustment, so
the site chosen for the freezer must have a flat, level floor.

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M1288-0070 U360 Ultra-Low Temperature Freezer User’s Manual
Position the freezer to allow free air entry through the intake grille and free air exit
from the back; a clearance of at least 6 inches (150 mm) on all sides is recommended to
prevent heat build-up in the compressor housing. The grille is located on the front of
both chest and upright models (see Figure 1). On upright models, the grille is located
behind the lower right front panel of the compressor housing.
For efficient temperature control, the freezer should be placed in a shaded area, away
from sources of excessive heat. For maximum cooling capability, the unit should be
located in an air-conditioned room.
3.3 Shelf Installation
Chest model C585 and C760 freezers cannot be fitted with shelves. The upright model
U101 freezer is factory-fitted with one fixed shelf.
Upright models U360, U535 and U725 have two adjustable shelves. These can be
positioned in 12.7 mm steps anywhere throughout the freezer. But to effectively utilize
racks within the freezer, be sure to position them so that each shelf is aligned with the
bottom of each inner door.
To install the shelves (model U360, U535, & U725 only):
1. To protect yourself, make sure the freezer is turned off and unplugged.
2. Remove the protective plastic coating from the shelf.
3. Position the four shelf clips evenly within the freezer by squeezing the clip, then
inserting it into the shelf support within the freezer.
4. Place the shelf in the freezer, making sure all four shelf clips are supporting the
weight of the shelf.
To readjust the shelf or shelf clips, gently squeeze the shelf clip to release it from the
side of the freezer, then reposition it as needed.
3.4 Connecting to the Power Supply
CAUTION!
BEFORE connecting the freezer to the electrical supply, make sure that
the power supply matches the requirements of the equipment. Check
the specification plate (located on the side of the freezer) for the
freezer’s electrical requirements.

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New Brunswick Scientific User’s Guide
3.4.1 Installing the Power Cord
Once you have verified that the power supply matches the electrical
requirements of the freezer, connect the unit to the power supply using the
power cord provided.
CAUTION!
If the plug on the power cord does not fit the outlet, or if the freezer’s
voltage rating does not match your electrical supply, do not plug the
freezer into the power supply. Contact your laboratory supervisor,
safety officer, or qualified service or electrical engineer.
#NOTE:
Some freezers are supplied with more than one removable power cord.
Utilize the cord that matches your power outlet.
1. Unwrap the power cord(s) provided. If you have more than one, select the
correct cord for your facility’s power outlet.
2. Install the rectangular end of the cord into the power outlet at the back of
the freezer.
3. Fasten the safety clip around the cord by tightening the screw located at the
top of the safety clip.
4. Plug the other end of the cord into an appropriately rated power outlet.
3.4.2 Power Switch Operation
The ON/OFF circuit breaker power switch is located within the lockable panel
(a) at the bottom lefthand corner on models U360, U535 & U725, or (b) at the
top righthand side on models U101, C585 & C760. The panel can be removed
by turning the key (provided) one quarter turn to the right. The key can be
removed to prevent access.
Set the ON/OFF power switch to the I(ON) position. The display illuminates
immediately.
#NOTE:
The freezer’s compressor will not operate for approximately one minute
after connection of the power supply, because there is an automatic delay
device in the circuit. Temperature and alarm settings, however, can be
adjusted immediately.

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M1288-0070 U360 Ultra-Low Temperature Freezer User’s Manual
3.5 Alarm Activation & Alarm Battery Charging
The equipment is delivered with the audible alarm & alarm battery deactivated. The
black battery rocker switch behind the lockable power switch panel activates the alarm
system battery. The switch is labelled I(ON)and O (OFF).
To activate the audible alarm and to charge the alarm battery, place the battery switch in
the I(ON)position. The alarm system battery will continuously charge when the battery
switch is in the ON position.
CAUTION!
Failure to turn on the battery switch may lead to a discharged battery,
low battery alarm indication, and/or a disabled alarm system.
The operation of the audible alarm can be verified at any time by pressing and holding
the TEST button within the DIAG menu of the display. The audible alarm should
sound.
#NOTE:
When the equipment is first switched on, it will take up to approximately
six hours to reach a temperature of -86°C, depending upon the size of the
freezer.
3.6 Remote Alarm Socket
The freezer is also fitted with a remote alarm socket (see Section 4.6 for details) for
connection to an external alarm monitoring system via the provided plug. The remote
alarm facility provides voltage-free contacts rated at 1 amp, 24 volts maximum. The
socket can be tested by turning off (O) the freezer’s main power switch or by pressing
and holding the TEST button within the DIAG menu of the display.
#NOTE:
In a power failure situation, the alarm socket will operature regardless of
the state of the alarm battery or battery switch.

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New Brunswick Scientific User’s Guide
3.7 Vacuum Effect
After closing the door or lid of the freezer, following access to the contents, a vacuum may
be created by the contraction of the warm air that entered the freezer. Before the door can be
opened again, it may be necessary to wait two or three minutes for the vacuum to be released
by the vacuum release vent tube. Do not try to force the door or lid.
During the release of the vacuum, a slight hissing may be heard. In order to minimize
vacuum formation, the vent contains a heater assembly to prevent ice buildup inside the vent
tube and has a spring-loaded plunger to clear ice from the internal opening of the vent.
#NOTE:
Be careful not to place a rack directly against the vent, as this will inhibit
the vent/plunger’s ability to operate correctly (see Section 5.1.4).

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4.1 Operating Features
Operating controls are located on a membrane panel mounted in the door of upright
models U360, U535 and U725. The same panel is located on the righthand side of all
chest freezers and the small U101 upright model.
Each Innova freezer is equipped with a sophisticated control system and large, backlit
LCD display that not only provides an immediate view of all freezer conditions, but
also provides access and display of programmable features, on-screen messaging,
alarm functions, service information and even help files (see Figure 2).
Figure 2: Operating Controls
4.2 Operational Controls
All adjustable setpoints and features are controllable through the display touchpad (see
Figure 2). Four directional keys allow features to be selected or modified while menu
keys provide access to specific functions listed at the bottom of the screen. Changes to
any menu can be saved by pressing the SAVE menu key. The EXIT menu key can be
pressed to return to the previous menu. If the EXIT menu key is pressed prior to
pressing the SAVE menu key, any changes made within that screen will not be
accepted.
T
EMPERATURE
SETPOINT
:
-80.0
PROG USER DATA DIAG HELP
LOW ALARM: -90.0
-86.5oC
Date: 06-30-04
Time: 16:23:35
HIGH ALARM: -75.0 NORMAL
SYSTEM STATUS
TEMPERATURE
SETPOINT
:
-80.0
PROG USER DATA DIAG HELP
LOW ALARM: -90.0
-80.5
o
C
Date: 06-30-04
Time: 16:23:35
HIGH ALARM: -75.0 NORMAL
SYSTEM STATUS
DIRECTIONAL
KEYPAD
MENU
SELECTIONS
MENU KEYPAD
This manual suits for next models
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Table of contents