Panasonic MCO-170AICL User manual

MCO-170AICL
MCO-170AICUVL
MCO-170AICUVHL
Please read the operating instructions carefully before using this product, and save the operating
instructions for future use.
See page 86 for all model numbers.
This product is for research use only.
Not for clinical diagnosis or treatment of humans or animals.
CO2Incubator
Operatin
g
Instructions

1
CONTENTS
INTRODUCTION P. 3
PRECAUTIONS FOR SAFE OPERATION P.4
LABELS ON INCUBATOR P. 8
ENVIRONMENTAL CONDITIONS P. 9
INCUBATOR COMPONENTS
Unit P.10
LCD touch panel P. 12
Remote alarm terminal P. 14
INSTALLATION
Installation site P. 15
Installation P. 17
Connecting CO2gas cylinder P. 19
BEFORE COMMENCING OPERATION
Initial cleaning method P. 20
Removing inner attachments P. 21
Installing inner attachments P. 23
Filling humidifying pan P. 24
FOR BETTER CULTIVATION
Precautions for cultures P. 25
Preventing contamination P. 26
CORRECT OPERATION P. 27
BASIC OPERATION ON LCD TOUCH PANEL P. 28
BASIC PARAMETERS
Numerical input to input window P. 30
Setting temperature, CO
2
density and high limit temperature alarm P. 31
Setting key lock P.33
Removing key lock P.35
ALARM PARAMETERS P. 36
OPERATION/ALARM LOG
Setting log interval P.39
Displaying operation log P.40
Exporting operation log P.43
Displaying alarm log P.46
Exporting alarm log P.48
OTHER PARAMETERS
Setting date and time P. 51
Setting brightness and sleep P. 52
Setting DAQ P. 54

2
CONTENTS
UV LAMP PARAMETERS P. 55
Using UV lamp P. 55
Setting UV lamp ON period P. 56
Lighting UV lamp for 24 hours P. 58
H2O2DECONTAMINATION P. 60
H
2O2decontamination P. 61
Precautions when handling H2O2reagent P. 65
Using unlock key P. 65
ELECTRIC LOCK (OPTION) P. 66
Setting auto lock P. 66
Using unlock key P. 67
Removing auto lock P. 68
GAS AUTO CHANGER (OPTION) P. 69
Connecting CO2gas cylinder P. 69
Automatic CO2gas supply line changeover P. 70
Manual CO2gas supply line changeover P. 72
STD GAS AUTO CALIBRATION KIT (OPTION) P. 73
ROUTINE MAINTENANCE P. 76
ALARMS, SAFETY, AND SELF-DIAGNOSIS P. 77
TROUBLESHOOTING P. 80
DISPOSAL OF UNIT P. 83
SPECIFICATIONS P. 84
PERFORMANCE P. 86
SAFETY CHECK SHEET P. 87

3
INTRODUCTION
■Read the operating instructions carefully before using the Product and follow the instructions for safety
operation.
■Our company disavows any responsibility for safety if the Product is used for other than the intended use
or used with any procedures other than those given in the operating instructions.
■Keep the operating instructions in a suitable place so that it can be referred to as necessary.
■The contents of the operating instructions are subject to change without notice for improvement of
performance or functions.
■Contact our sales representative or agent if any page of the operating instructions is lost or the page
order is incorrect.
■Contact our sales representative or agent if any point in the operating instructions is unclear or if there
are any inaccuracies.
■No part of the operating instructions may be reproduced in any form without the expressed written
permission of our company.
■This product is for research use only. Not for clinical diagnosis or treatment of humans or animals.
CAUTION
Our company guarantees the product under certain warranty conditions. Our company in no
way shall be responsible for any loss of content or damage of content.

4
PRECAUTIONS FOR SAFE OPERATION
It is imperative that the user complies with the operating instructions
as it contains important safety advice.
Items and procedures are described so that you can use this unit correctly and safely. If
the precautions advised are followed, this will prevent possible injury to the user and any
other person.
Precautions are illustrated in the following way:
WARNING
Failure to observe WARNING signs could result in a hazard to personnel
possibly resulting in serious injury or death.
CAUTION
Failure to observe CAUTION signs could result in injury to personnel and
damage to the unit and associated property.
Symbol shows;
this symbol means caution.
this symbol means an action is prohibited.
this symbol means an instruction must be followed.
Be sure to keep the operating instructions in a place accessible to users of this unit.
< Label on the unit >
This mark is labeled on the cover in which the electrical components of high voltage are
enclosed to prevent the electric shock.
The cover should be removed by a qualified engineer or a service personnel only.
WARNING
As with any equipment that uses CO2gas, there is a likelihood of oxygen depletion in the vicinity
of the equipment. It is important that you assess the work site to ensure there is suitable and
sufficient ventilation. If restricted ventilation is suspected, then other methods of ensuring a
safe environment must be considered. These may include atmosphere monitoring and warning
devices.

5
Do not use the unit outdoors. Current leakage or electric shock may result if the unit is exposed to
rain water.
Only qualified engineers or service personnel should install the unit. The installation by
unqualified personnel may cause electric shock or fire.
Install the unit on a sturdy floor and take an adequate precaution to prevent the unit from
turning over. If the floor is not strong enough or the installation site is not adequate, this may result in
injury from the unit falling or tipping over.
Never install the unit in a humid place or a place where it is likely to be splashed by water.
Deterioration of the insulation may result which could cause current leakage or electric shock.
Never install the unit in a flammable or volatile location. This may cause explosion or fire.
Never install the unit where acid or corrosive gases are present as current leakage or electric
shock may result due to corrosion.
Always ground (earth) the unit to prevent electric shock. If the power supply outlet is not
grounded, it will be necessary to install a ground by qualified engineers.
Never ground the unit through a gas pipe, water main, telephone line or lightning rod. Such
grounding may cause electric shock in the case of an incomplete circuit.
Connect the unit to a power source as indicated on the rating label attached to the unit. Use of
any other voltage or frequency other than that on the rating label may cause fire or electric shock.
Never store volatile or flammable substances in this unit if the container cannot be sealed. These
may cause explosion or fire.
Do not insert metal objects such as a pin or a wire into any vent, gap or any outlet on the unit.
This may cause electric shock or injury by accidental contact with moving parts.
Use this unit in safe area when treating the poison, harmful or radiate articles. Improper use
may cause bad effect on your health or environment.
Turn off the power switch (if provided) and disconnect the power supply to the unit prior to any
repair or maintenance of the unit in order to prevent electric shock or injury.
Do not touch any electrical parts (such as power supply plug) or operate switches with a wet
hand. This may cause electric shock.
WARNING

6
PRECAUTIONS FOR SAFE OPERATION
Ensure you do not inhale or consume medication or aerosols from around the unit at the time of
maintenance. These may be harmful to your health.
Never splash water directly onto the unit as this may cause electric shock or short circuit.
Never put containers with liquid on the unit as this may cause electric shock or short circuit when
the liquid is spilled.
Never bind, process, or step on the power supply cord, or never damage or break the power
supply plug. A broken supply cord or plug may cause fire or electric shock.
Do not use the supply cord if its plug is loose. Such supply cord may cause fire or electric shock.
Never disassemble, repair, or modify the unit yourself. Any such work carried out by an
unauthorized person may result in fire, or electric shock or injury due to a malfunction.
Disconnect the power supply plug if there is something wrong with the unit. Continued
abnormal operation may cause electric shock or fire.
When removing the plug from the power supply outlet, grip the power supply plug, not the cord.
Pulling the cord may result in electric shock or fire by short circuit.
Disconnect the power supply plug before moving the unit. Take care not to damage the power cord.
A damaged cord may cause electric shock or fire.
Disconnect the power plug when the unit is not used for long periods. Keeping the connection
may cause electric shock, current leakage, or fire due to the deterioration of insulation.
If the unit is to be stored unused in an unsupervised area for an extended period, ensure that children
do not have access and that doors cannot be closed completely.
The disposal of the unit should be accomplished by appropriate personnel. Remove doors to
prevent accidents such as suffocation.
Do not put the packing plastic bag within reach of children as suffocation may result.
Use the reagent specified by our company for H2O2decontamination. Using a different H2O2
solution may result in explosion or damage to the incubator.
When performing H2O2decontamination, securely close the internal and external doors. Failure to
do so may be harmful to health due to leakage of H2O2gas.
During H2O2decontamination, plug the access hole with the silicon cap that is provided. Failure to
do so may be harmful to health due to leakage of H2O2gas.
Always use the removal power supply cord that is provided. Other power supply cord may cause
electric shock or fire.
WARNING

7
This unit must be plugged into a dedicated circuit protected by branch circuit breaker.
Use a dedicated power source as indicated on the rating label attached to the unit. A multiple-tap may
cause fire resulting from abnormal heating.
Never store corrosive substances such as acid or alkali in this unit if the container cannot be
sealed. These may cause corrosion of inner components or electric parts.
Check the setting when starting up of operation after power failure or turning off of power
switch. The stored items may be damaged due to the change of setting.
Be careful not to tip over the unit during movement to prevent damage or injury.
Prepare a safety check sheet (copy the last page) when you request any repair or maintenance for
the safety of service personnel.
Wear rubber gloves when handling the H2O2reagent. Direct contact with the H2O2reagent may
result in inflammation of the skin.
H2O2decontamination can be performed only for the chamber and chamber attachments with standard
specifications, and not for any other objects.
Perform H2O2decontamination with the chamber attachments arranged as specified by our
company. Arranging them in a different way may result in insufficient decontamination.
After H2O2decontamination has been completed, wear rubber gloves and use a non-woven cloth to
wipe off the residual H2O2fluid from the bottom of the chamber, any objects that were
decontaminated, and the bottoms of ducts.
CAUTION

8
LABELS ON INCUBATOR
Warning and caution labels are attached to the incubator. The following table describes the labels.
This label is attached to covers that access high-voltage electrical components to
prevent electric shock. Only a qualified engineer or service personnel should be
allowed to open these covers.
This symbol indicates an ultraviolet light (UV) caution.
This symbol indicates that caution is required. Refer to product documentation for
details.
This symbol indicates a hot surface.
This symbol indicates an earth.
This symbol means “ON” for a power switch.
This symbol means “OFF” for a power switch.

9
ENVIRONMENTAL CONDITIONS
This equipment is designed to be safe at least under the following conditions (based on the IEC-61010-1):
■Indoor use;
■Altitude up to 2000 m;
■Temperature 5oC to 40oC
■Maximum relative humidity 80% for temperature up to 31oC decreasing linearly to 50% relative humidity
at 40oC;
■Mains supply voltage fluctuations up to ±10% of the nominal voltage;
■Transient overvoltages up to the levels of OVERVOLTAGE CATEGORY Ⅱ;
■Temporary OVERVOLTAGES occurring on the mains supply;
■Applicable pollution degree of the intended environment (POLLUTION DEGREE 2 in most cases);

10
INCUBATOR COMPONENTS
Unit
1 2 3 Tray 4 5
6
7※1
8
13
12*
2Electric key*211 10 9
*1: MCO-170AICUVHL/170AICUVL or when an
optional UV system set MCO-170UVS is installed.
*2: MCO-170AICUVHL or when an optional electric
lock MCO-170EL is installed.
20
21
Access port
cover Silicon cap
Rear left
19
Leveling
feet
Leveling
Foot
Handles
(When 13 is removed)
Sample air outlet cap
18 17 16※115 14
Removal power
supply cord port
Lower right side
(When some inner
attachments are removed)

11
1. Outer door: The outer door is held to the frame with the magnetic seal. The door heater is installed in
the door panel. The door opening is reversible. Contact our sales representative or agent to change the
door hinge from left to right or vice versa.
2. Inner door: The inner door is made of tempered glass. However do not subject the glass to excessive
impacts.
3. Tray catches: Insert tray to fit the concave portion on chamber.
4. Fan cover: The fan cover serves as the inlet for circulating air. It is removable.
5. Duct: The duct for the path for circulating air. It is removable.
6. Fan (inside the duct): The fan is made from polypropylene resin. It can be disinfected in an autoclave.
7. UV lamp*1: This UV lamp does not generate ozone. Never look directly at the UV light. For replacement,
contact our sales representative or agent.
8. Humidity control bar: Reduce automatically dew condensation occurred by the effect of outside
environment and the frequency of door opening/closing.
The humidity control bar has bactericidal effects by plated surface of it. However, it is recommended to
replace the humidity control bar every 5 to 6 years to maintain bactericidal effects. (The duration
of bactericidal effects differs depending on the use environment.)
9. Humidifying pan cover: This cover prevents the UV light entering the chamber. Always use it. Using
without it may have a bad influence on the chamber temperature distribution and humidity recovery.
10. Humidifying pan: Fill the humidifying pan with sterile distilled water. Install the humidifying pan in a
longitudinal direction as its shorter side is placed in the back.
11. Door switch: Detects the door opening/closing and stops the fan and electromagnetic valve for CO2
when the door is open. The UV lamp*1is also deactivated by the door opening.
12. Key hole*2:This is the hole to unlock with unlock key while outer door is locked by electric lock.
13. Switch cover: Prevent the accident of gas tube disconnected by the unexpected touch or power off.
14. Power supply cord cover plate: This plate is to prevent the power cord being come off.
15. Connecting port A for CO2gas pipe: Refer to page 19 for gas cylinder connection. Ensure that the
gas pressure is set at 0.03 MPa(G) (0.3 kgf/cm2(G), 4.3 psi(G)).
Note: When the optional MCO-21GC gas auto changer is installed, both ports A and B are available. Refer
to page 69 for gas auto changer.
16. Glow starter*1: The glow is started for the UV lamp. (Type FG-7P or FG-7PL)
17. Power switch: This is the main switch for the incubator. It also functions as an overcurrent breaker.
18. Sample air outlet: The sample air outlet also functions as an internal gas outlet. Normally, cover this
outlet with the sample air outlet cap.
19. USB port: Insert USB memory to export operations and alarms log. Refer to page 39～50.
Note: It is impossible to use USB memory which is required password input.
20. Access port: Place the silicon caps on both outside and inside of the port when the port is not being
used.
21. Remote alarm terminals: This terminal informs the alarm to remote location by connecting to external
alarm unit. Refer to page 14.

12
INCUBATOR COMPONENTS
LCD touch panel
The following display (called the Top screen) will appear when the power switch is turned ON.
Note: It takes approximately 20 seconds until Top screen is displayed.
2 6 7 8
3
1
1. Present temperature display field
The current chamber temperature is displayed.
2. Set temperature value display field
The set value of chamber temperature is
displayed. Default setting: 37 oC
3. Heating indicator
This lamp lights when the heater is energized.
4. Present CO2density display field
The current chamber CO2density is displayed.
Nothing is displayed when CO2 density is set 0%.
5. Set CO2density value display field
The set value of the chamber CO2density is
displayed. Default setting: 0 %
6. CO2gas injection indicator
This lamp lights when CO2gas is being
injected.
7. CO2gas supply line indicator A and B*1
Current supplying CO2gas supply line
(connecting port for CO2gas pipe) is
displayed. The connecting port A/B for gas
pipe that is currently supplying CO2is
displayed in reverse video and blinks.
8. CO2gas supply line select key*1
This is a key to select CO2gas supply line A
or B (Connecting port A or B for CO2gas
pipe). When an optional gas auto charger
MCO-21GC is installed, CO2 gas supply line
A/B changes over automatically when CO2
gas cylinder is empty. Changeover is also
workable by pressing this key.
*1: Only when an optional component MCO-21GC (Gas auto charger) is installed, this key is workable. They
are not displayed when the MCO-21GC is not installed.
5
4

13
10 11 9 12
13
18 17 16 15
9. Present date/time display field
Normally, this indicator shows date and time.
The date and time is simply set when the
incubator is shipped from the factory. Refer to
page 51 for details.
10. Over heat display
High limit temperature alarm is activated:
”Over Heat” is displayed alternately in normal
characters and reverse video.
11. UV lamp condition display
UV lamp ON: “UV : On” is displayed.
UV lamp OFF: ”UV : Off” is displayed.
Note: Nothing is displayed when an optional
UV system set MCO-170UVS is not installed
to the MCO-170AICL.
12. Outer door (opening/closing) display
Open: ”Door : Open” is displayed alternately
in normal characters and reverse video.
Close: ”Door : Closed” is displayed.
Locked: ”Door : Locked” is displayed.*2
13. Message display field
Alarms, errors or messages are displayed
when fault occurs. Refer to page 77～79.
Note: When there are a number of
alarms/errors, the display shows the message.
For example, if 2 alarms/errors occur in total,
the display shows “1/2”.
14. Message select key
When there are a number of alarm/erroros,
the message on the screen is changeable.
15. Menu key
Press this key to lead the Menu screen. It is
possible to set various setting on the Menu
screen. Refer to page 28.
*2: Auto lock function by electric lock is workable under any of the following conditions. When the condition
is not fulfilled, “Door : Locked” or Unlock key are not displayed.
・MCO-170AICUVHL. ・When an optional electric lock MCO-170EL is installed.
14

14
INCUBATOR COMPONENTS
16. H2O2key*3
This key is to run H2O2 decontamination. Refer
to page 60 to 65.
17. Unlock key*2
Press this key is to unlock the outer door when
it is auto-locked by electric lock. Refer to page
66 to 68. When the auto lock function is OFF,
this key is not displayed.
18. Buzzer key
Press this key to silence the buzzer. However,
when the ring back is ON, the buzzer will
sound again when the ring back passed and
the alarm state still continues. Refer to page
37～38 and 77～79.
Note: It is not possible to silence the buzzer
for the high limit temperature alarm.
*3: The H2O2 decontamination function is workable under any of the following conditions. When the condition is
not fulfilled, the H2O2 key is not displayed on the LCD touch panel.
・When an H2O2generator MCO-HP is installed in the MCO-170AICUVHL.
・When all H2O2generator MCO-HP, H2O2decon board MCO-170HB and electric lock MCO-170EL are
installed in the MCO-170AICUVL.
・When all UV system set MCO-170UVS, H2O2generator MCO-HP, H2O2decon board MCO-170HB and
electric lock MCO-170EL are installed in the MCO-170AICL.
Remote alarm terminal
The alarm of this product can be informed at a remote location from this product by connecting the external
alarm unit to the remote alarm terminals. For the type and behavior of remote alarm output, refer to page
77 to 79.
The terminal of the remote alarm is installed at the
rear upper right of the unit (See the figure on the
point). The alarm is outputted from this terminal.
Contact capacity is DC 30 V, 2 A.
When Buzzer key is pressed, the behavior of the
remote alarm is showed in Table.1.
Note: In the door alarm, the remote alarm does not
work. Refer to page 77 to 78.
Table 1 The behavior of the remote alarm when pressing the Buzzer key
Remote Alarm setting
(Refer to page 36～38)
Connecting
terminal
Normal
condition
Abnormal condition
(Including in the cases of power outage and
of where the power plug is pulled out.)
When pressing the buzzer key
ON:
Non-interlock with Buzzer key
COM.-N.C. Close Open Open (Maintain in abnormality)*
COM.-N.O. Open Close Close (Maintain in abnormality)*
OFF:
Interlock with Buzzer key
COM.-N.C. Close Open Close (Return to normal)
COM.-N.O. Open Close Open (Return to normal)
*In case of Err01 (CO2 gas cylinder empty）, Err11, 12(CO2 sensor error), the condition returns to normal.
Remote alarm
terminal

15
INSTALLATION
Installation site
For correct operation of the incubator, install it in a location with the following conditions.
WARNING
When using CO2gas for control, make sure that there is an adequate ventilation. Using CO2gas in a
small room without adequate ventilation may cause gas poisoning or oxygen deprivation. In addition, when
opening the incubator doors, do not directly inhale the air in the chamber.
Si l’appareil est utilisé dans un evdroit restreint, le niveau de la densite CO2de l’air peut s’élever et peut
être nocif aux humains. Evitez d’aspirer l’air provenant de l’inérieur de l’appareil quand vous ouverz la
porte.
Normal air environment
Install the incubator in an environment with normal air.
Do not expose to direct sunlight
Do not install the incubator in a location where it will be exposed to direct sunlight. If the incubator is
operated in direct sunlight, performance will be adversely affected.
Separate from heat sources
Do not install the incubator near significant heat sources, such as heaters, boilers, ovens, or autoclaves.
Heat will adversely affect the performance of the incubator.
Ambient temperature at least 5 C lower than set temperature
The control temperature of the incubator is at least 5 C higher than the ambient temperature. For example,
if the chamber is controlled at 37 C, the ambient temperature must be 32 C or less. Do not allow the
ambient temperature to become too high.
Strong and level floor
Select a site with a strong and level floor. If the floor is uneven or the installation is not level, the incubator
will be unstable and this may cause accident or injury. To avoid vibration and noise, always make sure that
the installation is stable. An unstable surface may result in vibration or noise.
WARNING
Install the incubator at a location that can support the weight. If the floor is not strong enough or if the
installation is insufficient, the incubator may fall over and cause injury.
Always make sure that the floor is strong, even, and level, and that the incubator will not tip over.
An insufficient installation may result in injury due to water leakage or the incubator falling over.
Low humidity
Select a site with a relative humidity of 80 %R.H. or lower. Using the incubator in high humidity may result
in current leakage or electric shock.
WARNING
Do not use the incubator outdoors. If the incubator is exposed to rain water, it may result in current
leakage or electric shock.
Never install the incubator in a moist location, such as near a sink or water line, or where it is likely
to be exposed to water. In addition, do not install it near water or steam pipes. Moisture can cause the
insulation to deteriorate, which may result in current leakage or electric shock.

16
INSTALLATION
No inflammable or corrosive gas
Never install the incubator in a location where it will be exposed to inflammable or corrosive gas. Doing so
may result in explosion or fire. In addition, insulation may deteriorate due to corrosion of protective casing,
resulting in current leakage or electric shock.
No falling objects
Do not install the incubator in a location where there is the possibility of objects falling from above. Doing
so may result in damage or accident.

17
Installation
1. Remove the packing tape and clean up.
Remove all the tapes that are securing the doors and the inner attachments. Open the doors for ventilation.
If the outer panels are dirty, wet a cloth with a diluted neutral detergent and wipe them. (Undiluted
detergent can damage the plastic components. For the dilution, refer to the instruction of the detergent.)
Wipe off the residual detergent with a wet cloth and then wipe off any moisture.
Note: Remove the cable tie banding the power supply cord. Prolonged banding may cause the corrosion
of the cord coating.
WARNING
Do not leave the plastic wrapping bags within reach of children. If the bag is placed over a child’s
head, it can block the mouth and nose and cause suffocation.
2. Adjust the leveling feet.
Adjust the leveling feet by turning them
counterclockwise to level the incubator. (See the
illustration on the right.)
Note: Incubating on a leaning tray may have a bad
influence on the cultivation.
3. Ground the incubator.
Ground the incubator during installation to prevent electric shock in case the insulation is not sufficient. If
there is no ground wire at the location, consult with qualified service personnel.
When a ground must be installed
If a grounded 3-pole outlet is not available, then a ground must be installed. Consult with qualified service
personnel.
WARNING
To prevent electric shock, always ground the incubator. If grounding is not possible, then have a ground
installed by qualified personnel. If the incubator is not grounded, it may result in electric shock.
Never connect the ground wire to a gas pipe, water pipe, lightning rod, or telephone ground wire.
Doing so may cause electric shock.
Installing a ground fault circuit breaker
If using the incubator in the location with moisture or humidity cannot be avoided, then it is recommended
that a ground fault circuit breaker be installed in the power supply circuit (i.e., the power supply at the
incubator). Have the circuit breaker installed by qualified service personnel.
Leveling feet
Shrink
Strech

18
INSTALLATION
CAUTION
Do not climb on the incubator or place objects on top of it. Doing so may damage it or cause it to fall
over, resulting in injury.
●In case of double stack
For stacking the incubators surely, refer to the
procedure included with the optional double
stacking bracket MCO-170PS or the stacking plate
MCO-170SB.
Note: Two hooks are attached to the rear of the
upper incubator. When stacking incubators, fix the
upper incubator to the wall with these hooks and
wire or chain (See the figure on the point).
Note: When stacking the incubators on our CO2
incubator or O2/CO2incubator other than this
product, use the stacking plate MCO-170SB. Refer
to table 11 on page 85.
When the incubator is not in use
Empty the water from the humidifying pan and remove moisture from the chamber. Make sure that the
chamber is completely dry before closing the doors. Failure to do so may result in damage.
Before moving the incubator
Before moving the incubator, empty the water from the humidifying pan, disconnect the power supply plug
from the outlet, and make sure that the cord will not be damaged. Failure to do so may result in electric
shock or fire.
Hooks
Wire

19
Connecting CO2gas cylinder
WARNING
When connecting a gas cylinder to the incubator, confirm the gas type. Confirm that the connections
are secure and that no gas will leak. Be sure to use the specified pressure. Using an incorrect gas or
pressure may result in explosion or fire, or in gas poisoning or oxygen deprivation due to qas leak.
Install the incubator in a location with adequate ventilation. If adequate ventilation cannot be provided,
then install an alarm system using CO2and O2 densitometers.
1. Get a CO2 gas cylinder ready and install an optional gas regulator MCO-100L.
Note:
・Use a liquefied CO2gas cylinder (at least 99.5 % pure). The siphon (dip tube) type cannot be used.
・When MCO-100L is not available, install a gas regulator rated at 25 MPa(G) (250 kgf/cm2(G), 3600
psi(G)) for the primary side, and 0.2 MPa(G) (2 kgf/cm2(G), 30 psi(G)) for the secondary side.
2. Using a gas tube that is provided, connect the
connecting port A for CO2gas pipe and the gas
regulator of the CO2gas cylinder.
For details on installing the optional automatic CO2
cylinder changeover kit (MCO-21GC), refer to page
69～71.
Note: If the CO2gas is supplied to multiple CO2incubators from a single gas cylinder, a CO2solid will be
formed in the gas regulator. The gas regulator safety valve will operate, and there may an explosive
sound.
3. After connecting the gas tube, make sure that no gas is leaking.
4. Set the CO2gas on the secondary side to 0.03 MPa(G) (0.3 kgf/cm2(G), 4.3 psi(G)) for gas injection.
Note: As the pressure increases, the CO2gas density control range will increase. Excessive pressure may
cause gas supply lines inside the incubator to come loose, which may result in gas poisoning or oxygen
deprivation due to gas leak. If gas lines come loose, the incubator must be repaired.
5. When there is no CO2 gas left and the CO2 gas empty alarm is activated, replace the empty gas cylinder
to a new one.
Note: When an optional gas auto changer MCO-21GC is installed, it switches the empty CO2 gas supply
line to the other automatically. Refer to page 69～71.
Note: The gas lines connected to the incubator will degrade over time. If any deterioration or abnormalities
are found during inspection, replace the lines immediately.
Connecting port A for CO2gas pipe
Lower right side