ThermoFisher Scientific CryoMed TSCM17XA Manual

Thermo ScientificTM
CryoMedTM Controlled-Rate Freezer
Operating and Maintenance Instructions
331486H11 • Revision C • 1/13/2022

IMPORTANT Read this instruction manual. Failure to follow the instructions in this manual can result in
damage to the unit, injury to operating personnel, and poor equipment performance.
CAUTION All internal adjustments and maintenance must be performed by qualified service personnel.
Material in this manual is for informational purposes only. The contents and the product it describes are
subject to change without notice. Thermo Fisher Scientific makes no representations or warranties with
respect to this manual. In no event shall Thermo Fisher Scientific be held liable for any damages, direct or
incidental, arising from or related to the use of this manual.
© 2022 Thermo Fisher Scientific Inc. All rights reserved.
For your future reference and when contacting the factory, have the following information readily
available. It can be found on the data plate attached to your unit.
Model Number:
Serial Number:
The following information, if available, is helpful for contacting the factory.
Date Purchased:
Purchase order number:
Source of Purchase:
(manufacturer or specific agent/rep organization)

Contents
Models......................................................................... 1
Safety Precautions....................................................... 3
Handling Liquid Nitrogen .......................................... 4
Unpacking ................................................................... 6
Packing List ............................................................. 6
Introduction ................................................................. 7
Intended Use............................................................ 7
Control Panel ........................................................... 7
Door Latch............................................................... 7
USB WiFi Dongle...................................................... 7
Thermal Printer (Optional) ......................................... 7
Operating Standards................................................ 8
Installation.................................................................... 9
Selecting a Location................................................. 9
LN2Connections...................................................... 10
Electrical Connections.............................................. 10
Sample Thermocouple Connection .......................... 10
Start Up....................................................................... 11
Power-Up ................................................................ 11
Connectivity Requirements....................................... 11
Initial Start Up........................................................... 12
Operation..................................................................... 17
Home Screen........................................................... 17
Settings.................................................................... 18
Logs......................................................................... 25

Profiles..................................................................... 27
Users ....................................................................... 30
Calibration................................................................ 32
Active Run Summary ................................................... 34
Initiate a Run ............................................................ 34
Manual Override....................................................... 34
Stop a Profile Run .................................................... 35
Run Review.............................................................. 35
Alarms ......................................................................... 37
General Alarm Guidelines ......................................... 37
Printer Set-Up.............................................................. 40
Change the Paper .................................................... 40
Specifications .............................................................. 41
Temperature Uniformity............................................ 41
Parts List ..................................................................... 43
Accessories ............................................................. 43
PC Interface Software.................................................. 45
Installation................................................................ 45
Login........................................................................ 47
Setup ....................................................................... 49
Run Operation.......................................................... 49
Typical Run Procedure ............................................. 52
Manual Override Procedure...................................... 53
End Run................................................................... 54
Print ......................................................................... 54
Alarms...................................................................... 55
Power Failure Recovery............................................ 56
Display/Control Summary......................................... 57

Edit Screen .............................................................. 57
Startup..................................................................... 57
Error Messages........................................................ 61
Review Screen ......................................................... 63
User Controls........................................................... 63
Options Screen ........................................................ 65
Plot Colors ............................................................... 65
Default Directories.................................................... 66
Users ....................................................................... 66
Status Bar................................................................ 68
Help ......................................................................... 69
About....................................................................... 69
Maintenance ................................................................ 70
Dry Gaskets and Heat Breaks .................................. 70
Disinfect Controlled-Rate Freezer Interior.................. 70
Clean the Cabinet Exterior........................................ 71
Solenoid Valve Maintenance..................................... 71
Reset Heater Over temp Thermostat........................ 71
Preventive Maintenance ........................................... 71
Troubleshooting........................................................... 73
Connectivity Troubleshooting ....................................... 75
Appendix A: User Role Summary ................................. 77
Appendix B: City Time Zone......................................... 78
Appendix C: OPC UA Server Information (Optional)...... 84

Warranty...................................................................... 88
Thermo Fisher Scientific PC
and Peripheral Warranty........................................... 88
Thermo Fisher Scientific International Dealer PC
and Peripherals Warranty ......................................... 88
Thermo Fisher Scientific
Controlled-Rate Freezer Warranty ............................ 88
Thermo Fisher Scientific International
Controlled-Rate Freezer Dealer Warranty.................. 89
WEEE Compliance....................................................... 90
Regulatory ................................................................... 91
Product Safety ......................................................... 91
Conformity with European Regulations..................... 91
21 CFR part 11 ........................................................ 91
FCC Statement (USA) .............................................. 91
Korea KC ................................................................. 92
Australi/New Zealand RCM ...................................... 92
Contact Information .................................................... 93

Controlled-Rate Freezer Models | 1
Models
The following table shows the different models covered in this Operating and Maintenance Manual.
Table 1. Applicable Models
Models Chamber
Volume Electrical Plug Type
Exterior
Dimensions
W x H x D
in. (cm)
Interior
Dimensions
W x H x D in.
(cm)
Option FLA
TSCM17XA
17L / 0.6 cu. ft.
120V / 60Hz NEMA 5-15P
37.3 x 21.7 x 24.3
(94.7 x 55.1 x 61)
7 x 12 x 13
(17.8 x 30.5 x 33) -
12.0
TSCM17XV 220-230V / 50/60Hz CEE 7/7 5.5
TSCM17XL 100V / 60Hz NEMA 5-15P 10.5
TSCM34XA
34L / 1.2 cu. ft.
120V / 60Hz NEMA 5-15P
43.3 x 21.7 x 24.3
(109.9 x 55.1 x 61)
13 x 12 x 13
(33 x 30.5 x 33) -
12.0
TSCM34XV 220-230V / 50/60Hz CEE 7/7 5.5
TSCM34XL 100V / 60Hz NEMA 5-15P 10.5
TSCM48XA
48L / 1.7 cu. ft.
120V / 60Hz NEMA 5-15P
49.3 x 21.7 x 24.1
(125.2 x 55.1 x 61)
19 x 12 x 13
(48.3 x 30.5 x 33) -
12.0
TSCM48XV 220-230V / 50/60Hz CEE 7/7 5.5
TSCM48XL 100V / 60Hz NEMA 5-15P 10.5
TSCM17PA
17L / 0.6 cu. ft.
120V / 60Hz NEMA 5-15P
37.3 x 21.7 x 24.3
(94.7 x 55.1 x 61)
7 x 12 x 13
(17.8 x 30.5 x 33)
Thermal
Printer
12.0
TSCM17PV 220-230V / 50/60Hz CEE 7/7 5.5
TSCM17PL 100V / 60Hz NEMA 5-15P 10.5
TSCM34PA
34L / 1.2 cu. ft.
120V / 60Hz NEMA 5-15P
43.3 x 21.7 x 24.3
(109.9 x 55.1 x 61)
13 x 12 x 13
(33 x 30.5 x 33)
Thermal
Printer
12.0
TSCM34PV 220-230V / 50/60Hz CEE 7/7 5.5
TSCM34PL 100V / 60Hz NEMA 5-15P 10.5
TSCM48PA
48L / 1.7 cu. ft.
120V / 60Hz NEMA 5-15P
49.3 x 21.7 x 24.3
(125.2 x 55.1 x 61)
19 x 12 x 13
(48.3 x 30.5 x 33)
Thermal
Printer
12.0
TSCM48PV 220-230V / 50/60Hz CEE 7/7 5.5
TSCM48PL 100V / 60Hz NEMA 5-15P 10.5
TSCM17SA 17L / 0.6 cu. ft. 120V / 60Hz NEMA 5-15P 37.3 x 21.7 x 24.3
(94.7 x 55.1 x 61)
7 x 12 x 13
(17.8 x 30.5 x 33) OPC UA 12.0
TSCM17FA 17L / 0.6 cu. ft. 120V / 60Hz NEMA 5-15P 37.3 x 21.7 x 24.3
(94.7 x 55.1 x 61)
7 x 12 x 13
(17.8 x 30.5 x 33)
OPC UA
Thermal
Printer
12.0
TSCM17SV 17L / 0.6 cu. ft. 220-230V / 50/60Hz CEE 7/7 37.3 x 21.7 x 24.3
(94.7 x 55.1 x 61)
7 x 12 x 13
(17.8 x 30.5 x 33) OPC UA
5.5
TSCM17FV 17L / 0.6 cu. ft. 220-230V / 50/60Hz CEE 7/7 37.3 x 21.7 x 24.3
(94.7 x 55.1 x 61)
7 x 12 x 13
(17.8 x 30.5 x 33)
OPC UA
Thermal
Printer
5.5

2 | Models Controlled Rate Freezer
TSCM34SA 34L / 1.2 cu. ft. 120V / 60Hz NEMA 5-15P 43.3 x 21.7 x 24.3
(109.9 x 55.1 x 61)
13 x 12 x 13
(33 x 30.5 x 33) OPC UA
12.0
TSCM34FA 34L / 1.2 cu. ft. 120V / 60Hz NEMA 5-15P 43.3 x 21.7 x 24.3
(109.9 x 55.1 x 61)
13 x 12 x 13
(33 x 30.5 x 33)
OPC UA
Thermal
Printer
12.0
TSCM34SV 34L / 1.2 cu. ft. 220-230V / 50/60Hz CEE 7/7 43.3 x 21.7 x 24.3
(109.9 x 55.1 x 61)
13 x 12 x 13
(33 x 30.5 x 33) OPC UA
5.5
TSCM34FV 34L / 1.2 cu. ft. 220-230V / 50/60Hz CEE 7/7 43.3 x 21.7 x 24.3
(109.9 x 55.1 x 61)
13 x 12 x 13
(33 x 30.5 x 33)
OPC UA
Thermal
Printer
5.5
TSCM48SA 48L / 1.7 cu. ft. 120V / 60Hz NEMA 5-15P 49.3 x 21.7 x 24.3
(125.2 x 55.1 x 61)
19 x 12 x 13
(48.3 x 30.5 x 33) OPC UA
12.0
TSCM48FA 48L / 1.7 cu. ft. 120V / 60Hz NEMA 5-15P 49.3 x 21.7 x 24.3
(125.2 x 55.1 x 61)
19 x 12 x 13
(48.3 x 30.5 x 33)
OPC UA
Thermal
Printer
12.0
TSCM48SV 48L / 1.7 cu. ft. 220-230V / 50/60Hz CEE 7/7 49.3 x 21.7 x 24.3
(125.2 x 55.1 x 61)
19 x 12 x 13
(48.3 x 30.5 x 33) OPC UA
5.5
TSCM48FV 48L / 1.7 cu. ft. 220-230V / 50/60Hz CEE 7/7 49.3 x 21.7 x 24.3
(125.2 x 55.1 x 61)
19 x 12 x 13
(48.3 x 30.5 x 33)
OPC UA
Thermal
Printer
5.5
Table 1. Applicable Models
Models Chamber
Volume Electrical Plug Type
Exterior
Dimensions
W x H x D
in. (cm)
Interior
Dimensions
W x H x D in.
(cm)
Option FLA

Controlled-Rate Freezer Safety Precautions | 3
Safety Precautions
In this manual, the following symbols and conventions are
used.
WARNING: This symbol when used alone
indicates important operating instructions which
reduce the risk of injury or poor performance of
the unit.
WARNING: This symbol indicates potentially
hazardous situations which, if not avoided, could
result in serious injury or death.
WARNING: This symbol indicates situations
where dangerous voltages exist and potential for
electrical shock is present.
CAUTION: This symbol, in the context of a
CAUTION, indicates a potentially hazardous
situation which if not avoided could result in
minor to moderate injury or damage to the
equipment. This indicates a situation which may
result in property damage.
CAUTION: Equipment being maintained or
serviced must be turned off or locked off to
prevent possible injury.
WARNING: Before installing, using or
maintaining this product, be sure to read the
manual and product warning labels carefully.
Failure to follow these instructions may cause the
product to malfunction, which could result in
injury or damage.
CAUTION: Contains Parts and Assemblies
Susceptible to Damage by Electrostatic
Discharge (ESD)
WARNING: The snowflake symbol indicates low
temperatures and risk of frost bite. Do not touch
bare metal or samples with unprotected body
parts.
WARNING: Potential asphyxiation hazard. This
equipment should be located in a well ventilated
room.
WARNING: This symbol indicates a need to use
gloves during the indicated procedures. If
performing decontamination procedures, use
chemically resistant gloves. Use insulated gloves
for handling samples and when using liquid
nitrogen.
WARNING: Unit weighs more than 50 lbs
(22.7 kg). Use proper 2 person lifting techniques
when handling.
CAUTION: This symbol indicates surfaces which
may become hot during use and may cause a
burn if touched with unprotected body parts.
WARNING: This symbol indicates potentially
hazardous situations, which if not avoided could
result in fire.
WARNING: Potential biological hazards. Proper
protective equipment and procedures must be
used.
WARNING: Whenever working with liquid
nitrogen storage equipment in a closed
environment, use of personal O2 detection
equipment is strongly recommended.
WARNING: Ultra low temperatures are
associated with this equipment. Instructions in
this section should only be carried out when
using special handling equipment or when
wearing special handling protective clothing.
WARNING: Extreme temperature hazards. Only
qualified persons should perform procedures
associated with this symbol.
Note Because argon is not an inert gas whose physical
properties are very similar to those of nitrogen,
the precautions and safe practices for the
handling and use of liquid argon are the same as
those for liquid nitrogen.
WARNING: The unit is equipped with six self-
leveling legs. Install the unit on the counter top,
ensuring all 6 legs are in contact with the counter.
When the unit door is open, do not lean against
or put weight on top of the open door, as doing
so may cause the unit to tip.
WARNING: All internal adjustments and
maintenance must be performed by qualified
service personal.

4 | Safety Precautions Controlled Rate Freezer
Handling Liquid Nitrogen
Handle liquid nitrogen carefully.
The extremely low temperatures can freeze human flesh very
rapidly. When spilled on a surface, the liquid tends to cover it
completely, cooling a large area. The gas issuing from the
liquid is also extremely cold. Delicate tissue, such as that of
the eyes, can be damaged by exposure to cold gas which
would be too brief to affect the skin of the hands or face.
Never allow any unprotected part of your body to touch
objects cooled by liquid nitrogen.
Note: Always wear gloves when handling anything that is, or
may have been, in immediate contact with liquid nitrogen.
Insulated gloves are recommended, but heavy leather gloves
may also be used. The gloves should fit loosely, so that they
can be thrown off quickly if liquid should splash into them.
Such objects may stick rapidly to the skin and tear the flesh
when you attempt to free yourself. Use tongs to withdraw
objects immersed in the liquid, and handle the object
carefully.
Wear protective clothing.
Protect your eyes with a face shield or safety goggles (safety
glasses without side shields do not give adequate protection).
When handling liquid in open containers, it is advisable to
wear high-top shoes. Trousers (which should be cuff less if
possible) should be worn outside the shoes.
Introduction
The safe handling and use of liquid nitrogen in cryogenic
freezer and dewar flasks is largely a matter of knowing the
potential hazards and using common-sense procedures
based on that knowledge. There are two important properties
of liquid nitrogen that present potential hazards:
1. It is extremely cold. At atmospheric pressure, liquid
nitrogen boils at –320°F (–196°C).
2. Very small amounts of liquid vaporize into large amounts
of gas. One liter of liquid nitrogen becomes 24.6 cu. ft.
(0.7 m3) of gas.
The safety precautions in this manual must be followed to
avoid potential injury or damage which could result from these
two characteristics. Do not attempt to handle liquid nitrogen
until you read and fully understand the potential hazards, their
consequences, and the related safety precautions. Keep this
information handy for ready reference and review.
Note: Because argon is an inert gas whose physical
properties are very similar to those of nitrogen, the
precautions and safe practices for handling and use of liquid
argon are the same as those for liquid nitrogen.
Use containers designed for low temperature liquids.
Cryogenic containers are specifically designed and made of
materials that can withstand the rapid changes and extreme
temperature differences encountered in working with liquid
nitrogen. Even these special containers should be filled
SLOWLY to minimize the internal stresses that occur when
any material is cooled. Excessive internal stresses can
damage the container.
Do not cover or plug the entrance opening of any liquid
nitrogen freezer or dewar.
Do not use any stopper or other device that would interfere
with venting of gas. These cryogenic liquid containers are
generally designed to operate with little or no internal
pressure. Inadequate venting can result in excessive gas
pressure which could damage or burst the container. Check
the unit periodically to be sure that venting is not restricted by
accumulated ice or frost.
Use proper transfer equipment.
Use a phase separator or special filling funnel to prevent
splashing and spilling when transferring liquid nitrogen into or
from a dewar. The top of the funnel should be partly covered
to reduce splashing. Use only small, easily-handled dewars
for pouring liquid. For the larger, heavier containers, use a
cryogenic liquid withdrawal device to transfer liquid from one
container to another. Be sure to follow instructions supplied
with the withdrawal device. When liquid cylinders or other
large storage containers are used for filling, follow the
instructions supplied with those units and their accessories.
Do not overfill containers.
Filling above the bottom of the neck-tube (or specified
maximum level) can result in overflow and spillage of liquid
when the neck-tube core or cover is placed in the opening.
Never use hollow rods or tubes as dipsticks.
When a warm tube is inserted into liquid nitrogen, liquid will
spout from the top of the tube due to gasification and rapid
expansion of liquid inside the tube.
WARNING: Contact of liquid nitrogen or cold
gas with the skin or eyes may cause serious
freezing (frostbite) injury.
WARNING: Nitrogen gas can cause suffocation
without warning. Store and use liquid nitrogen
only in a well-ventilated place.

Controlled-Rate Freezer Safety Precautions | 5
As the liquid evaporates, the resulting gas tends to displace
normal air from the area. In closed areas, excessive amounts
of nitrogen gas reduce the concentration of oxygen and can
result in asphyxiation. Because nitrogen gas is colorless,
odorless and tasteless, it cannot be detected by the human
senses and will be breathed as if it were air. Breathing an
atmosphere that contains less than 18% (or) 20% oxygen can
cause dizziness and quickly result in unconsciousness and
death. Use of oxygen monitoring equipment is strongly
recommended.
Note: The cloudy vapor that appears when liquid nitrogen is
exposed to the air is condensed moisture; not the gas itself.
The issuing gas is invisible.
Never dispose of liquid nitrogen in confined areas or places
where others may enter. Disposal of liquid nitrogen should be
done outdoors in a safe place. Pour the liquid slowly on gravel
or bare earth where it can evaporate without causing
damage. Do not pour the liquid on pavement.
First Aid
If a person seems to become dizzy or loses consciousness
while working with liquid nitrogen, move to a well-ventilated
area immediately. If breathing has stopped, apply artificial
respiration. If breathing is difficult, give oxygen. Call a
physician. Keep warm and at rest.
If exposed to liquid or cold gas, restore tissue to normal body
temperature (98.6°F) as rapidly as possible, followed by
protection of the injured tissue from further damage and
infection.
Remove or loosen clothing that may constrict blood
circulation to the frozen area. Call a physician. Rapid warming
of the affected part is best achieved by using water at 108°F.
Under no circumstance should the water be over 112°F, nor
should the frozen part be rubbed either before or after
rewarming.

6 | Unpacking Controlled Rate Freezer
Unpacking
At delivery, examine the exterior for physical damage while the
carrier’s representative is present. If exterior damage is
present, carefully unpack and inspect the unit and all
accessories for damage.
If there is no exterior damage, unpack and inspect the
equipment within 15 days of delivery. If you find any damage,
keep the packing materials and immediately report the
damage to the carrier.
Do not return goods to the manufacturer without
written authorization.
When submitting a claim for shipping damage, request that
the carrier inspect the shipping container and equipment.
Packing List
The following items are packed along with the unit:
• USB that contains:
- Installation and Operation manual.
- PC Interface 3.3
• Essential Safety Instructions
• 2 ML Int Sample T/C Probe
• 9 Pin DB9 10 ft. Ext Cable
• Standard Ampules Stopper
• Straw Stopper
• 6 Ft LN2Transfer Hose SS
• USB to Serial Adapter
• USB WiFi Dongle
• DPU-S445 User's Guide Disk (Optional)- accessible with
thermal printer models
Remove the Shipping
Brackets
Using a 7/16" wrench or socket, remove the (4) lag screws
attached to the shipping pallet. Remove the (4) 1/4-20 bolts
to detach the (2) shipping brackets from the bottom of the
unit. Refer to the Figure 1 below.
Figure 1. Bottom view of the unit
Note: These are the unpackaged weights of the unit.
CAUTION: Use proper 2-person lifting
techniques when handling the unit (refer Ta b le 2
for approximate unit weight).
CAUTION: Do not rest the unit on its back as it
may damage the plumbing or plastic heat break.
WARNING: Carefully remove the shipping
brackets. Some edges may be sharp.
Ta b l e 2 . U n i t w e i g h t s
Model lbs. (kg) 3Model lbs. (kg)
TSCM17PA 155 (70.3) TSCM17XA 154 (69.8)
TSCM17PV 155 (70.3) TSCM17XV 154 (69.8)
TSCM17PL 155 (70.3) TSCM17XL 154 (69.8)
TSCM17FA 155 (70.3) TSCM17SA 154 (69.8)
TSCM17FV 155 (70.3) TSCM17SV 154 (69.8)
TSCM34PA 175 (79.3) TSCM34XA 174 (78.9)
TSCM34PV 175 (79.3) TSCM34XV 174 (78.9)
TSCM34PL 175 (79.3) TSCM34XL 174 (78.9)
TSCM34FA 175 (79.3) TSCM34SA 174 (78.9)
TSCM34FV 175 (79.3) TSCM34SV 174 (78.9)
TSCM48PA 192 (87.0) TSCM48XA 191 (86.6)
TSCM48PV 192 (87.0) TSCM48XV 191 (86.6)
TSCM48PL 192 (87.0) TSCM48XL 191 (86.6)
TSCM48FA 192 (87.0) TSCM48SA 191 (86.6)
TSCM48FV 192 (87.0) TSCM48SV 191 (86.6)
Shipping
Bracket Bolts Lag Screw

Controlled-Rate Freezer Introduction | 7
Introduction
Intended Use
Thermo Scientific CryoMed Controlled-Rate Freezers are
sample preparation devices used for the preparation of
samples for storage at ultra-low or cryogenic temperatures
(–80°C to –196°C).
The Controlled-Rate Freezers are intended for use:
• As a sample preparation device in research use.
• For preparation of samples not intended to be
re-introduced into the human body such as primary cell
lines or stem cells for research.
Non-Intended Use
These CryoMed Controlled-Rate Freezers are not intended to
be operated in potentially explosive environments and are not
intended for storage of flammable inventory. It is not
considered as a medical device and has not been registered
with a medical device regulatory body (e.g. FDA); i.e, it has
not been evaluated for the storage of samples for diagnostic
use or for samples to be reintroduced to the body.
Figure 2. CryoMed Controlled-Rate Freezer
ON/OFF
The right side of the unit is equipped with an ON/OFF switch
as shown in Figure 2.
Control Panel
A 7" touch screen user interface is provided at front of the unit
as shown in Figure 2 to control the unit.
Door Latch
The door of the Controlled-Rate Freezer is secured in the
closed position with a vise-action T-handle latch as shown in
Figure 2. The latch maintains positive compression of the
door gasket against the Teflon heat break to prevent leakage
and ice build-up during normal operation. The latch is closed
by turning the handle 180° clockwise.
Make sure that the door is latched properly during operation
to prevent ice build-up.
USB WiFi Dongle
A USB WiFi dongle will be shipped with the unit, which allows
you to connect to a user accessible WiFi network (WPA2,
Enterprise) and establish a connection to Thermo Connect.
Check Connectivity Requirements for more details.
Thermal Printer (Optional)
There is a printer on top of the CryoMed Controlled-Rate
Freezer as shown in Figure 2. Printer provides a visual record
of a profile run, including any errors/alarms which might have
occurred. Run data is captured upon run completion, which
can then be printed from the Run Review screen.
Thermal Printer (Optional)
USB WiFi Dongle
Soft Shut Down
USB Port
ON/OFF Switch
Control Panel
Door Handle
Power ON switch
Power OFF switch

8 | Introduction Controlled Rate Freezer
Operating Standards
These units are designed to operate under the following
environmental conditions:
• Indoor use
• Altitude up to 2000 m
• Ambient temperature: 15°C to 35°C
• Humidity: 20%~85%, non-condensing
• Mains Supply Fluctuations: ±5% of nominal for better
performance
• Installation Category II
• Pollution Degree 2
• Class of Equipment I

Controlled-Rate Freezer Installation | 9
Installation
Selecting a Location
A six foot liquid nitrogen transfer hose with 1/2" flare fittings is
supplied with the unit. The use of a transfer hose longer than
six feet may degrade system performance as it may prevent
liquid nitrogen from reaching the unit in the volume needed to
properly control the temperature profile run.
The unit should be located within the reach of the transfer
hose to the liquid nitrogen supply, allowing enough space for
source tank replacement. A gauge to measure the remaining
contents in the source tank should be easily viewable and
read before each run to ensure that enough liquid nitrogen is
available for successful temperature profile completion.
A dedicated source tank is recommended for optimum
performance.
Unit should be located in a well-ventilated area of the
laboratory. Place the unit on a table/bench which is capable
of supporting the unit’s weight (see Tab le 2 ), with adequate
workspace available. Allow clearance for the door on the front
of the unit, for the hose and power connections on the back
and the exhaust vent on the left side of the unit. Refer to
Figure 3 for rear side connections.
Behind the electronics housing is a 1/4" OD overflow drain
tube from the drain pan placed under the inlet solenoids.
Although most of the frost will melt and evaporate within the
drain pan, it is possible that a small amount of water may drip
out of the drain tube.
Figure 3. Rear view of the unit
WARNING: Nitrogen gas can cause suffocation
without warning. Store and use liquid nitrogen
only in a well-ventilated place. As the liquid
evaporates, the resulting gas displaces the
normal air in the area. (The cloudy vapor that
appears when liquid nitrogen is exposed to the
air is condensed moisture, not the gas itself. The
issuing gas is invisible.) In closed areas,
excessive amounts of nitrogen gas reduces the
concentration of oxygen and can result in
asphyxiation. Because nitrogen gas is colorless,
odorless, and tasteless, it cannot be detected by
the human senses. Breathing an atmosphere that
contains less than 19.5% (or) 20% oxygen can
cause dizziness and quickly result in
unconsciousness and death. Therefore, the use
of oxygen monitoring equipment is strongly
recommended.
WARNING: Contact of liquid nitrogen with the
skin or eyes may cause serious (freezing) injury.
CAUTION: Use proper 2-person lifting
techniques when handling the unit (refer to
Specifications section for approximate unit
weight.
WARNING: Do not block nor tamper with the
exhaust vent opening! Provide ample clearance
around the exhaust vent to allow the gases to
escape freely, otherwise an increase of gas
pressure inside the chamber will forcibly open the
door possibly causing damage to the unit and
risking sample integrity.
CAUTION: If moisture cloud is seen exiting the
drain tube, turn off the unit, shut off the liquid
nitrogen supply source valve, and immediately
call for service.
Fan
PC Interface (RS232)
Power Inlet
Safety Valve
LN2Connection
Drain Tube
Remote Alarm
OPC UA (Optional) port

10 | Installation Controlled Rate Freezer
LN2Connections
The unit requires a user-supplied, clean, low pressure
regulated (22 PSIG + 30%) liquid nitrogen supply. Pressure
higher than recommendations may degrade unit performance
as these could hold the fill solenoids partially open. A 60 PSIG
liquid nitrogen pressure relief valve is located on the back of
the unit (Figure 3).
Connect the transfer hose between the low pressure, liquid
outlet of the nitrogen supply tank (not the gas outlet) and the
LN2supply inlet located on the back of the unit Figure 3. The
unit is shipped with a removable plastic protective cap over
the LN2supply inlet to keep debris out of the solenoids and
protect the inlet threads during shipping and handling. Do not
over-tighten the fitting as it may damage the internal
plumbing. Arrangements should be made to collect the water
which will form on the transfer hose from melted frost, to
prevent a potential slip or fall hazard.
After the transfer hose has been connected, open the supply
valve and check the connections for leaks.
Electrical Connections
See the serial tag on the side of the unit for electrical
specifications.
1. Verify the ON/OFF switch on the right side of the unit is
OFF.
2. Connect the appropriate line cord to the power inlet on
the rear of the unit.
3. Connect the unit to a grounded, dedicated circuit.
Sample Thermocouple
Connection
1. Open the unit door.
2. Locate the thermocouple connector in the front upper
right corner of the chamber.
3. Install the supplied sample thermocouple in the correct
orientation.
4. Close the unit door.
Note: The freezer has been calibrated at the factory with the
1.2/2.0 ml sample thermocouple probe installed in the internal
thermocouple connector. Refer Calibration section to
calibrate an external thermocouple probe.
Figure 4. Sample Thermocouple Connection
CAUTION: The flair connections on the ends of
the transfer hose do not require any sealant. Pipe
dope or Teflon®tape may cause contamination
of the liquid fill solenoids or leaks at the hose
connections because these cannot withstand the
extreme low temperatures of this application.
CAUTION: Do not disable or remove the
pressure relief valve.
CAUTION: The ON/OFF switch is the main
disconnect device for the unit. Position the unit
so the switch is easily accessible.

Controlled-Rate Freezer Start Up | 11
Start Up
Power-Up
1. Turn on the Controlled-Rate Freezer power switch on the
right side of the unit.
2. The startup screen will be displayed.
3. Wait for the Start Setup button to appear.
Note: The LED to the right of the UI should be green.
Connectivity
Requirements
There are two requirements to take full advantage of your
Controlled-Rate Freezer connectivity options:
1. The Controlled-Rate Freezer can be connected to the
internet, using wireless connection only.
a. Refer to WiFi Specification table for acceptable
network security parameters.
b. Speak with your local Information Technology (IT)
group about the correct wireless network and
password to use.
2. You must have a Thermo Fisher Connect account to link
the unit to his/her account via InstrumentConnect™. To
create a Thermo Fisher Connect account:
a. Use a web browser to open the following URL:
https://apps.thermofisher.com
b. Choose “Create an Account” and follow the
instructions to establish a new account. (Remember
your login information for later use.)
c. (Optional) Download the InstrumentConnect mobile/
tablet application from the AppStore or PlayStore.
Note: InstrumentConnect™ allows you to monitor all
connected instruments. To access this section, click on the
InstrumentConnect icon indicated below.
Figure 5. InstrumentConnect Screen
Note: Users outside U.S.A, Canada and Europe cannot
access Connectivity with WiFi Dongle.
Note: Thermo Fisher Scientific connected devices support
certification validation.
If your Controlled-Rate Freezer is unable to connect to either a
wireless network or a cloud account, verify your name and
password, and re-connect.
If issues persist, contact our local support.
Note: If your network password expires or changes, your
Controlled-Rate Freezer will no longer connect automatically.
Be sure to manually update your password in the Controlled-
Rate Freezer user interface settings to reconnect. Failure to
reconnect prior to completing a profile run will result in data
loss.
Table 3. WiFi Specification table
Parameter Specification
Default WiFi transmission
frequency to the cloud
Once after every profile run
for run file transmission.
WiFi protocol 2.4 GHZ only
IEEE 802.11b/g/n
WiFi Security WPA2PSK
WPA2PEAP(PEAP)
WiFi Data Rate Minimum of 1 Mbps
WiFi Range Up to 30 meters
Minimum Required
Wireless Signal –67 dbM
Firewall Ports that must
open 123, 443

12 | Start Up Controlled Rate Freezer
Initial Start Up
To start up the Controlled-Rate Freezer, complete the
following steps:
• Once the Controlled-Rate Freezer is powered up, the
Thermo Scientific logo is displayed on the front screen. If
this is the first time the unit is being turned on, an initial
setup must be completed. Press the Start Setup button
to initiate the setup.
Figure 6. Main Screen
The first step of the setup is to select the language. This
screen allows you to specify the preferred display language.
Once the language is selected, press the Next button.
Figure 7. Language Selection Screen
The next screen allows you to select your location. Enter the
name of the country and the city and select from the list of
suggestions displayed.
After selecting the region, press the Next button.
Figure 8. Region Setup Screen
When entering your “City” into the Region Setup screen, refer
to “Appendix B: City Time Zone”. Follow the table to select
the city closest to your time zone. The next screen allows you
to identify an individual Controlled-Rate Freezer by specifying
a Unit Name. After entering a name, press the Next button.
Figure 9. Unit Name Setup Screen
The next few screens allow you to setup a wireless
connection to store information on InstrumentConnect.
To fully connect your new unit, you will need to:
1. Connect the unit to a wireless network allowing data to
be sent to Thermo Fisher Connect.
2. Log in to Thermo Fisher Connect (web or app) and link
the unit to your account via InstrumentConnect.
Note: Before you begin, establish a Thermo Fisher Connect
account and have your network login information available.
Refer to Power-Up section for details.

Controlled-Rate Freezer Start Up | 13
Accept the terms and conditions for using connectivity on this
device.
Figure 10. Terms and conditions screen
If your unit has OPC UA enabled or you have selected country
region where WiFi is not approved for use, then connectivity
settings are not available.
If you wish to use connectivity and your unit is OPC UA
enabled, contact service.
If you chose to Skip this acceptance, WiFi connectivity will be
disabled. You will be able to accept the terms and enable
connectivity at a later time via the Connectivity settings menu.
If you skip the acceptance you will be navigated to the Date
Setup screen.
Select the preferred date format. Once the date is selected
(highlighted), press the Next button.
Figure 11. Date Setup Screen
The next screen allows you to setup the Time.
Select the preferred time format. Once the intended time is
selected (highlighted), press the Next button.
Figure 12. Time Setup Screen
If you accept the terms and conditions of using connectivity
on this device you will be navigated to Wireless Setup.
Select a wireless network and press the Next button.
Figure 13. Wireless Setup Screen
Once the intended network is selected (highlighted), you must
press the Next button.
The following instructions are for WPA2 connection type.
Enter the network password and press the Next.
Figure 14. Wireless Setup (Password) Screen

14 | Start Up Controlled Rate Freezer
Note: Press the eye icon on the screen for password
visibility
A success message is displayed once the connection is
established. If the connection fails, a general error message is
displayed. It could be due to an incorrect WiFi password or
network incompatibility. After 5 seconds, the interface will
return to the previous screen.
If this occurs, check the network status and re-enter the
correct password to establish the connection. (Tip: Use the
eye icon to verify the password entry before attempting
connection.)
Refer to the Connectivity Troubleshooting section for more
connectivity issues.
Figure 15. Wireless Setup Error Message Screen
The Network Configuration screen appears after a successful
connection is established, which displays the time and date
based on the wireless network. Verify the information
displayed and press Next.
Figure 16. Network Configuration Screen
The next screen displays two options to connect your unit to
InstrumentConnect. You can store historical data and receive
alarm notifications to your InstrumentConnect account.
Figure 17. Connect to the InstrumentConnect
Screen
Connect via Mobile Device
Selecting the Connect via Mobile Device option displays the
following screen. Follow the instructions to connect to
InstrumentConnect.
Figure 18. Connect to the InstrumentConnect (via
Mobile Device) Screen
Figure 19. InstrumentConnect Mobile Device
Screens