VDW nuve DirectFREEZE DF 290 User manual

NÜVE SANAYİ MALZEMELERİ İMALAT VE TİCARET A.Ş.
DF 290 / DF 490 / DF 590
DirectFREEZE™
ULTRA LOW TEMPERATURE
DEEP FREEZER
INSTRUCTION MANUAL
1984
Z14.K 25 276 Rev.No: 08 Rev. Date: 01/2018

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Dear Nüve User,
We would like to take this opportunity to thank you for preferring this Nüve product. Please
read the operating instructions carefully and keep them handy for future reference.
Please detain the packing material until you see that the unit is in good condition and it is
operating properly. If an external or internal damage is observed, contact the transportation
company immediately and report the damage. According to ICC regulations, this responsibility
belongs to the customer.
While you are operating the instrument please;
1. obey all warning labels,
2. do not remove warning labels,
3. do not operate damaged instrument,
4. do not operate instrument with a damaged cable,
5. do not move instrument during operation.
In case of a problem contact your Nüve agent for an authorized service or maintenance.
The validity of the guarantee is subject to compliance with the instructions and precautions
described in this manual.
Nüve reserves the right to improve or change the design of its products without any obligation
to modify previously manufactured products.
Information contained in this document is the property of Nüve. It may not be duplicated or
distributed without our permission.
NÜVE
SANAYİ MALZEMELERİ
İMALAT VE TİCARET A.Ş.
Saracalar Mahallesi, Saracalar Kümeevleri No:4/2
Akyurt 06750 Ankara / TURKEY
Tel : [phone] (pbx)
Fax : [phone]
Sales : [email protected]r
Technical Service: [email protected]

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WARRANTY CERTIFICATE
1. Nüve warrants that the equipment delivered is free from defects in material and
workmanship. This warranty is given for a period of two years. The warranty period begins
from the delivery date.
2. Warranty does not apply to parts normally consumed during operation or general
maintenance or any adjustments described in the operating instructions provided with the
instrument.
3. Nüve does not accept any liability in case where the goods are not used in accordance with
their proper intent.
4. The warranty may not be claimed for damages incurred during the shipment, for damages
resulting from improper handling or use, abuse, fire, liquid spillage, tampering or
unauthorized repairs by any persons, use of defective or incompatible accessories, exposure
to abnormally corrosive conditions, use of the product in non-standard environmental
conditions, including but not limited to failure to meet requirements of ambient
temperature, lubrication, humidity or magnetic field influences, from the defects in
maintenance, negligence, bad functioning of auxiliary equipment, in the case of force
majeure or accident and incorrect power supply.
5. Any injury, loss or damage caused; due to a failure resulting from negligence of the
instructions given in this manual; is beyond the scope of the warranty conditions.

BEFORE OPERATING THE INSTRUMENT THIS MANUAL SHOULD BE READ CAREFULLY.

THE VALIDITY OF THE GUARANTEE IS SUBJECT TO THE OBSERVATION OF THE
INSTRUCTIONS AND PRECAUTIONS DESCRIBED IN THIS MANUAL.
INFORMATION CONTAINED IN THIS DOCUMENT IS THE PROPERTY OF NÜVE. IT MAY
NOT BE DUPLICATED OR DISTRIBUTED WITHOUT PERMISSION.
PLEASE REGISTER ONLINE TO VALIDATE YOUR WARRANTY:
To register your warranty online, please visit our webpage www.nuve.com.tr and fill in the
“Warranty Registration Form”.

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CONTENT
Page
INTRODUCTION ............................................................................................................................... 5
1.1. USE AND FUNCTION ................................................................................................................. 5
TECHNICAL SPECIFICATIONS ............................................................................................................ 6
2.1. TECHNICAL SPECIFICATIONS TABLE........................................................................................... 6
2.2. ACCESSORIES............................................................................................................................ 7
2.2.1. FACTORY FITTED ACCESSORIES ......................................................................................... 7
2.2.2. OPTIONAL ACCESSORIES ................................................................................................... 7
PRECAUTIONS AND LIMITATIONS ON USE ....................................................................................... 7
SYMBOLS AND LABELS..................................................................................................................... 8
INSTALLATION ................................................................................................................................. 9
ENVIRONMENTAL CONDITIONS ................................................................................................ 9
HANDLING AND TRANSPORTATION ........................................................................................ 10
UNPACKING............................................................................................................................ 10
MAINS SUPPLY........................................................................................................................ 10
POSITIONING.......................................................................................................................... 10
GENERAL PRESENTATION ....................................................................................................... 11
5.6.1. CHAMBER DIMENSIONS.................................................................................................. 12
CONTROL PANEL..................................................................................................................... 12
PRIOR TO OPERATION............................................................................................................. 14
OPERATING PRINCIPLES................................................................................................................. 14
PROGRAMMING..................................................................................................................... 14
6.1.1. OPERATING MODE.......................................................................................................... 14
6.1.2. SETTINGS ........................................................................................................................ 15
6.1.3. MEMORY ........................................................................................................................ 16
INTERNAL MEMORY ................................................................................................ 17
EXTERNAL MEMORY (USB STICK)............................................................................. 18
6.1.4. SMS ................................................................................................................................ 19
6.1.5. E-MAIL............................................................................................................................ 20
6.1.6. SET TEMPERATURE ......................................................................................................... 21
6.1.7. GRAPH WORKING SCREEN .............................................................................................. 21
6.1.8. TEMPERATURE ALARM RANGE........................................................................................ 21
PERIODIC MAINTENANCE AND CLEANING...................................................................................... 22
PERIODIC MAINTENANCE ....................................................................................................... 22
CLEANING............................................................................................................................... 22
DISPOSAL MANAGEMENT CONCEPT .............................................................................................. 23
TROUBLESHOOTING ...................................................................................................................... 23
ERROR CODES......................................................................................................................... 23
FUSE REPLACEMENT............................................................................................................... 24
OPTIONS ................................................................................................................................... 24
AlerText™ GSM MODULE .................................................................................................... 24
NüveWARN™ REMOTE ALARM SYSTEM ............................................................................... 25
10.2.1. MOUNTING REMOTE ALARM ...................................................................................... 25
CHART RECORDER............................................................................................................... 26
CO2BACK-UP UNIT.............................................................................................................. 26
ELECTRICAL CIRCUIT DIAGRAM.................................................................................................. 28
DF 290, DF 490 AND DF 590 ELECTRICAL CIRCUIT DIAGRAM ............................................... 28

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INTRODUCTION
1.1. USE AND FUNCTION
DF series Ultra Low Temperature Freezer is designed for the storage of the samples needed to
store at ultra-low temperature and it is used in the hospitals, blood banks, epidemic diseases
research centers, research laboratories in the universities, genetic engineering and bio-
pharmaceutical industry.
DF Series Ultra Low Temperature Freezers are capable of cooling down to set points between -
45°C and -90°C with 1°C sensitivity and keep temperature stable within given tolerances. The
live of the most of the biological samples stop at -70°C. These samples can be stored for a long
time without loss of their vital functions by using Ultra-low temperature freezers. DF series
protect living cells and tissues for a long time without any destruction.
The refrigerant gas passes through evaporators placed under each shelf, bottom of the chamber
and top of the chamber; thus, each compartment of freezer is cooled directly. DF series
provides a stronger and more rapid cooling with its DirectFREEZE™technology. Refrigeration
system is cascade system in DF series. The refrigeration is provided by non-explosive refrigerant
gas which does not include CFC.
The power switch is equipped with a key lock to prevent unauthorized usage. Door handle on DF
series can be opened easily. Moreover, silicon gasket placed on the door protects air leakage.
DF series Ultra-Low Temperature Freezer has audible and visual alarm systems for the following
cases; high and low temperature conditions, power failure, temperature sensor failure and
other faults related with the microprocessor control system. DF series store last 50 failures in
the memory and these errors can be followed on the display. Standard Ethernet connection
feature enables email sending to five different e-mail addresses in case of any error. DF series
offer AlerText™GSM module as an option and SMS can be sent to five different mobile phone
numbers in case of error.
The display is fed by an automatically re-chargeable battery in case of power failure. Therefore,
temperature in the chamber is displayed in case of electricity interruption. Remote alarm outlet
and the outlet for connection to central alarm system are offered as standard.
DF series ultra-low temperature freezers have internal memory which can store temperature
values for 10 years. In addition to internal memory, temperature values can be recorded in USB
stick. DF series have a weekly circular chart recorder as an option. The temperature in the
chamber is recorded by this chart recorder operated by the backup battery. Chart recorder is
equipped with a key lock to prevent unauthorized usage.
CO2back-up system is an optional accessory. This system provides to keep temperature stable
at -60°C in case of power failure or compressor failure. Thus, samples in the chamber are
protected against temperature increase.

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DF Series Freezers are designed and manufactured in accordance with national and
international directives, EN 50419 and EN 61000-6-3 standards under the supervision of total
quality management systems ISO 9001 and ISO 13485.
This device is in compliance with WEEE Regulation.
If the warnings mentioned in this manual are not considered, NUVE will not be responsible
from their results.
TECHNICAL SPECIFICATIONS
2.1. TECHNICAL SPECIFICATIONS TABLE
TECHNICAL SPECIFICATIONS
DF 290
DF 490
DF 590
Freezing System
DirectFREEZE™
Temperature Sensor
Pt-100
Temperature Set and Reading Sensitivity
1°C
Temperature Set Range
-45°C to -90°C
Temperature Variation (on samples)
< 2.0°C
< 3.0°C
Chamber Volume (Liter)
261
461
560
Refrigerants
R507 - R508B
Remote Alarm and Alarm Back-up
12-hours with automatically recharged battery
Alarm System
Audible and Visual
Temperature Alarm Range
Set point +5°C to +25°C
Safety System
Pressure Switch (Automatic reset),
Pressure Switch (Manuel reset)
Insulation
130 mm injected polyurethane block (CFC-free)
Chamber Configuration (independent
compartment / internal insulation door)
3 / 3
4 / 4
Control System
N-SmArt™ Microprocessor
Temperature Indicator
Colorful LCD
Temperature Recorder (Optional)
Battery operated 7-day chart with key lock
Internal Memory
10-year memory, one record per hour
USB
Standard
Ethernet
Standard
RS 232 / GSM
Standard
Central Alarm Outlet
Standard
CO2Back-up system
Liquid CO2
Power Supply
230 V, 50 Hz
Power Consumption (Watt)
1600
Internal Material
Stainless steel
External Material
Epoxy-polyester powder coated stainless steel
Internal Dimensions (WxDxH) mm
420x625x995
555x630x1320
555x770x1320
External Dimensions (WxDxH) mm
680x1070x1715
820x965x2035
815x1120x2010

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2.2. ACCESSORIES
2.2.1. FACTORY FITTED ACCESSORIES
DF XXX Y Weekly temperature chart recorder 0°C / -100°C
2.2.2. OPTIONAL ACCESSORIES
A 08 191 AlerText™GSM alarm module
K 13 009 NuveWarn™ Remote alarm system with 10 m cable
A 08 195 NuveCloser™ Software CD with 3 m.RS 232 cable
A 08 135 CO2back-up unit
A 08 137 Rack for 135 x 135 x 50 mm PP cryoboxes (DF 290 and DF 490)
A 08 138 PP Cryobox 52 x 133 x 133mm capacity 9 x 9 tubes
A 08 150 Rack with three drawers (DF 290 and DF 490)
A 08 151 Rack for 135x135x75 mm cryoboxes (DF 290 and DF 490)
A 08 152 Rack with two drawers (DF 290 and DF 490)
A 08 155 Cryobox 135x135 mm Height 75 mm
A 08 158 10x10 Divider for up to 12 mm Ø tubes. Divider height: 65 mm
A 08 159 9x9 Divider for up to 13.6 mm Ø tubes. Divider height: 65 mm
A 08 160 Diagram paper for chart recorder 0°C / -100°C (Pack of 100)
A 08 165 PP Cryobox 75x133x133 mm capacity 9x9 tubes
A 08 171 Cryobox 135x135 mm Height 50 mm
A 08 172 10 x 10 Divider for up to Ø 12 mm tubes. Divider height: 30 mm
A 08 173 9 x 9 Divider for up to 13.6 mm Ø tubes. Divider height: 30 mm
A 08 180 Rack for 135x135x50 mm cryoboxes (DF 290 and DF 490)
A 08 196 Rack with two drawers (DF 590)
A 08 197 Rack with three drawers (DF 590)
A 08 198 Rack for 133 x 133 x 75 mm cryoboxes (Rack capacity: 20 boxes) (DF 590)
A 08 199 Rack for 137 x 137 x 50 mm cryoboxes (Rack capacity: 25 boxes) (DF 590)
A 08 200 Rack for 135 x 135 x 50 mm PP cryoboxes (Rack capacity: 25 boxes) (DF 590)
A 08 070 Spare pen for chart recorder

Please contact NÜVE for different size of cryoboxes and racks.
PRECAUTIONS AND LIMITATIONS ON USE
The user shall pay attention to the following:
Do not operate the instrument for purposes other than its main purpose.
The instrument should only be used by authorized and trained staff after the instruction manual has
been read carefully. Only authorized technical staff can handle the product in case of any failure.
Only original spare parts and original accessories supplied by Nüve should be used.
Correctly grounded power supply should be used.
There should not be any material in the chamber that can damage to the device.
The set temperature should not destroy the structure of the samples without user’s notice.
The maximum capacity of each compartment is 40 kg. Do not load the freezers more than the
capacities in the compartment.
Open the cover of the compartment that is used. Otherwise, temperature fluctuation may be
observed.

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SYMBOLS AND LABELS
Symbol in the operating instructions:
Attention, general hazard area.
This symbol refers to safety relevant warnings and indicates possibly dangerous
situations. The non-adherence to these warnings can lead to material damage
and injury to personal.

Symbol in the operating instructions:
This symbol refers to important circumstances.
Notified Body:
KIWA
Belgelendirme Hizmetleri A.Ş.
(İTOSB) İstanbul Tuzla Organize Sanayi Bölgesi Tepeören Mevkii
34597 Tuzla- İstanbul / TURKEY
Before operating the instrument this manual should be read carefully.
Type B protection.
This product is subject to the directive 2002/96/EC on Waste Electrical and
Electronic Equipment (WEEE) of the European Parliament and of the EU Council
of Ministers.
Labels on the product:
Earthed
Wall
Sockets
Remote alarm output
Normally closed contact
output
DF 290 / DF 490/ DF 590
Fuses (2 x 13A)

9
INSTALLATION
ENVIRONMENTAL CONDITIONS
The instrument is designed to operate safely under the following conditions:
Indoor use only
Ambient temperature: 5°C to 30°C
Maximum relative humidity for temperature up to 31°C: 80 %
Maximum altitude: 2000 m
Temperature for maximum performance: 15°C / 25°C
If ambient temperature is higher than 30°C, freezer may not operate properly and
irreparable damages may occur.
The freezer operates at lower temperatures than -80 °C at maximum 20°C ambient
temperature.

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HANDLING AND TRANSPORTATION
All handling and transportation must be carried out by using proper equipment and experienced
staff. The instrument must be supported underneath, moved in vertical position and never be
turned upside down.
UNPACKING
Remove the cardboard box packing and ensure that no damage has occurred during
transportation. Remove the second nylon wrapping around the instrument. The below
mentioned are provided with the instrument, please check them;
1 ea. user’s manual and warranty
1 ea. power switch key
2 ea. door handle key
MAINS SUPPLY
The instrument requires 230 V, 50 Hz.
Please make sure that the supplied mains matches the required power ratings which are written
on the name plate of the instrument located at the back of the instrument.
Always plug-in the instrument to correctly grounded sockets.
A supply fitted with a circuit breaker should be used for protection against indirect
contact in case of isolation fault.
POSITIONING
Check that the positioning is suitable for the users.
Check that the instrument is stable on its four feet.
Check that the user will be able to follow up the operation even when he deals with
something else.
Check that the positioning of the device prevents interference with other equipment in the
near surrounding.
Ensure that the instrument is not exposed to sunlight directly.
Leave at least 30 cm free space between the device and wall due to ventilation of
compressor.