AKTA design P-900 User manual

User Manual
18-1120-04
Pump P-900
18-1120-04_AB.qxd 1998-10-19 15:37 Sida 1 (1,1)

Trademarks
ÄKTA and UNICORN are trademarks of Amersham
Pharmacia Biotech Limited or its subsidiaries.
Amersham is a trademark of Nycomed Amersham plc.
Pharmacia and Drop Design are trademarks of
Pharmacia & Upjohn Inc.
Terms and Conditions of Sale
All goods and services are sold subject to the terms
and conditions of sale of the company within the
Amersham Pharmacia Biotech group which supplies
them. A copy of these terms and conditions of sale is
available on request.
Adresses
Amersham Pharmacia Biotech UK Limited
Amersham Place
Little Chalfont
Buckinghamshire
England HP7 9NA
Amersham Pharmacia Biotech AB
SE-751 84 Uppsala
Sweden
Amersham Pharmacia Biotech Inc
800 Centennial Avenue
PO Box 1327
Piscataway
NJ 08855 USA
© Amersham Pharmacia Biotech AB 1998
– All rights reserved
Important user information
Meaning: Consult the instruction manual to avoid
personal injury or damage to the product or other
equipment.
WARNING!
The Warning sign is used to call attention to the
necessity to follow an instruction in detail to avoid
personal injury. Be sure not to proceed until the
instructions are clearly understood and all stated
conditions are met.
CAUTION!
The Caution sign is used to call attention to
instructions or conditions that shall be followed to
avoid damage to the product or other equipment. Be
sure not to proceed until the instructions are clearly
understood and all stated conditions are met.
Note
The Note sign is used to indicate information
important for trouble-free or optimal use of the
product.
Should you have any comments on this instruction, we
will be pleased to receive them at:
Amersham Pharmacia Biotech AB
SE–751 84 Uppsala
Sweden
!
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Contents
1 Introduction
1.1 General ..................................................................................................................1
1.2 Safety.....................................................................................................................3
2 Installation
2.1 Unpacking..............................................................................................................4
2.2 General precautions ...............................................................................................4
2.3 Connecting electrical signal cables .........................................................................4
2.4 Connecting to communication link........................................................................5
2.5 Connecting to supply voltage.................................................................................5
2.6 Installing the piston seal rinsing system .................................................................6
2.7 Connecting the inlet tubing....................................................................................7
2.8 Purging the pump ..................................................................................................7
2.9 Running-in the new pump .....................................................................................8
2.10 Connecting the pump outlet tubing .......................................................................9
2.11 Installation of accessories.......................................................................................9
3 Operation
3.1 On/off..................................................................................................................11
3.2 Menu selection and settings .................................................................................11
3.3 Main menu overview ...........................................................................................12
3.4 Starting and stopping the pump...........................................................................13
3.5 Setting the flow rate and starting the pump.........................................................14
3.6 Setting concentration B........................................................................................14
3.7 Running a simple gradient ...................................................................................15
3.8 Ending the run and storage..................................................................................15
3.9 Changing eluent ..................................................................................................16
3.10 Restart after power failure...................................................................................16
4 Maintenance
4.1 Periodic maintenance ...........................................................................................17
4.2 Cleaning-in-place .................................................................................................17
4.3 General care.........................................................................................................17
4.4 Replacing the piston seal .....................................................................................18
4.5 Running-in a new piston seal...............................................................................22
4.6 Replacing a damaged piston ................................................................................22
4.7 Cleaning the inlet and outlet check valves ...........................................................23
Pump P-900
User Manual 18-1120-04 Edition AB i
Contents
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5Trouble shooting
5.1 General ................................................................................................................25
5.2 Faults and actions................................................................................................25
5.3 Error messages.....................................................................................................26
5.4 Checking the pump pressure................................................................................27
5.5 Removing trapped air bubbles from the pump ....................................................29
Reference information
A Description ..........................................................................................................30
A.1 Instrument.................................................................................................30
A.2 Pump head ................................................................................................31
A.3 Pump principle..........................................................................................34
A.4 Outlet manifold.........................................................................................35
A.5 Pressure monitoring ..................................................................................35
A.6 Using an external chart recorder ...............................................................35
B Menus..................................................................................................................36
B.1 Check menu ..............................................................................................36
B.1.1 Checking piston stroke .............................................................36
B.1.2 Check pump operation time .....................................................36
B.1.3 Check number of piston strokes ...............................................36
B.1.4 Check Service Mode .................................................................36
B.2 Setup menu ...............................................................................................37
B.2.1 Set pressure limit.......................................................................37
B.2.2 Set pressure full scale on recorder.............................................37
B.2.3 Set pressure unit........................................................................37
B.2.4 Set gradient base.......................................................................37
B.2.5 Set gradient mode .....................................................................37
B.2.6 Set pump type...........................................................................38
B.2.7 Set compression compensation..................................................38
B.2.8 Set mixer operation...................................................................39
B.2.9 Set valve operation....................................................................39
B.2.10 Setup language..........................................................................39
B.2.11 Setup unit number ....................................................................39
B.2.12 Setup display angle ...................................................................39
B.3 Alarm timer...............................................................................................40
B.4 Service displays .........................................................................................40
B.5 Menu overview .........................................................................................41
C Technical specifications .......................................................................................42
D Accessories and spare parts..................................................................................45
Short instructions on back page
About this manual
This manual comprises two parts; a practical part (sections 1 – 5)
and a reference part (sections A – D). Sections 1 – 5 contain the
necessary information for operating the instrument.
Pump P-900
User Manual 18-1120-04 Edition AB
ii
Contents
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1 Introduction
1.1 General
Pump P-900 is the collective name for a pump family. The family
members are:
• P-901 stand-alone or system mounted
Used as stand-alone, or in ÄKTA™ explorer 100. Equipped with
four pump heads in two pump modules.
• P-903 stand-alone or system mounted
Used as stand-alone, or in ÄKTA™ purifier 10/10 XT and
ÄKTAexplorer 10/10 XT. Equipped with four pump heads in two
pump modules.
Pump P-900
User Manual 18-1120-04 Edition AB 1
Introduction 1
Fluid inlet tubing
Out to system
P-900
P-901, P-903
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Pump P-900
User Manual 18-1120-04 Edition AB
2
All P-900 models are high performance laboratory pumps for use in
liquid chromatography and other applications where accurately
controlled liquid flow is required. Double mode can be selected to
double the flow rate range.
Pump P-900 features:
• P-901
- Pressure range 0 – 10 MPa (100 bar, 1450 psi).
- Flow rate range 0.01 – 100 ml/min isocratic and gradient mode.
- Double mode flow rate range of 0.01 – 200 ml/min isocratic
mode.
• P-903
- Pressure range 0 – 25 MPa (250 bar, 3625 psi).
- Flow rate range 0.001 – 10 ml/min isocratic and gradient mode.
- Double mode flow rate range of 0.001 – 20 ml/min isocratic
mode.
• The pumps are equipped with 2 pump modules, A and B,
including two pump heads each. This allows for binary gradients
with high pressure mixing.
• All pumps have a pressure sensor connected to pump module A.
• Low pulsation.
• Switch-valve control for low pressure gradients and BufferPrep.
All versions are run with the same software. Normal or double
mode, as well as the size of the pump heads are stated in a Setup
meny, see Reference information B.2.
Accessories
Accessories can be connected to the pumps, and can be controlled
from the pumps or from a PC running UNICORN™version. 2.10 or
higher.
These accessories are:
• Mixer M-925.
• 2-way Switching Valve SV-903.
1 Introduction
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1.2 Safety
•The instrument is designed for indoor use only.
• Do not use in a dusty atmosphere or close to spraying water.
• Do not block the air inlet and outlet of the unit.
Pump P-900
User Manual 18-1120-04 Edition AB 3
Introduction 1
WARNING! Always disconnect the power supply before
attempting to replace any item on the instrument.
WARNING! Incorrectly fitted tubing may loosen, causing a jet of
liquid to spray out. This is especially dangerous if hazardous
chemicals are in use. Connect the tubing by first inserting the
tubing fully, then tightening the connector fingertight. Finally
tighten the connector a further 1/4 turn using the key supplied.
WARNING! When using hazardous chemicals, all suitable
protective measures, such as protective glasses, must be taken.
WARNING! The instrument must not be opened by the user. It
contains high voltage circuits which can give a lethal electric shock.
WARNING! The instrument must be connected to a grounded
mains socket.
WARNING! NaOH is injurious to health. Avoid spillage.
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Pump P-900
User Manual 18-1120-04 Edition AB
4
2 Installation
2 Installation
2.1 Unpacking
Unpack the instrument and check the items against the supplied
packing list. Inspect the items for obvious damage which may have
occurred during transportation.
It is recommended that all packing materials should be retained if
onward transport of the instrument is expected.
2.2 General precautions
The instrument should be installed in a non-corrosive atmosphere.
The instrument should be located in a place of low temperature
variations, away from heat sources, draughts and direct sunlight.
The instrument may be operated at normal ambient temperatures in
the range +4 to +40 °C.
The instrument should be installed on a stable laboratory bench, in
ÄKTAexplorer or in ÄKTApurifier. To ensure correct ventilation a
free space of 0.1 m is required behind and in front of the instrument.
Place the instrument directly on the bench. Do not use any soft
material under the instrument, to ensure that the ventilation inlet in
the front is not blocked.
2.3 Connecting electrical signal cables
The sockets for electrical signals are located on the rear panel.
Valve 1
Valve A
Valve B
UniNet 2
Pressure,
analog
out 0 - 1V UniNet 1 Mains
Remote
CAUTION! The following information should be read carefully
to ensure that the instrument is installed correctly.
P-900
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Pump P-900
User Manual 18-1120-04 Edition AB 5
Installation 2
Connecting to chart recorder (if used)
1 Connect the chart recorder to the Mini-DIN-socket Pressure
using the cable supplied.
Pin Signal
1 Signal
2 Signal ground
3–6 Not used (Reserved for factory testing)
Note: The signal cable is delivered with protective covers on each wire.
Do not remove the protective covers from unused connections as a
short circuit may disturb the measurements.
2 Set the recorder to 0–1 V input, full scale.
3 Refer to section B.2.2 for a description of setting the full scale
pressure.
2.4 Connecting to communication link
The pumps used in ÄKTAexplorer and ÄKTApurifier, are controlled
from a PC running UNICORN version 2.10 or higher, using UniNet
cables.
1Connect two UniNet cables to the UniNet 1 connectors. The
instrument can be connected in series anywhere in the chain
between the PC and the termination plug. The UniNet 1 link
connects, in series, the PC with Pump P-900 and other
instruments. The termination plug is connected to the last
instrument in the chain.
2.5 Connecting to supply voltage
1Make sure the on/off switch is in the OFF-position O.
2 Connect a mains cable between the instrument and a grounded
mains socket. The instrument is delivered with both European
and US type mains cables, as standard. Any voltage from
100–240 V AC, 50–60 Hz can be used.
The instrument contains no user replaceable fuse.
CAUTION! The mains power to ÄKTAexplorer and ÄKTApurifier
must be switched OFF before connecting the instrument to the
UniNet 1 link.
WARNING! The instrument must be connected to a grounded
mains socket.
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2.6 Installing the piston seal rinsing system
Note: For the protection of the new pump, do not run the pump
until the system has been flushed out and primed with eluent,
according to sections 2.8 and 2.9.
The rinsing system for the piston seals must be installed before use.
20% ethanol should be passed around the piston and piston seal on
the non–pressure side of the piston seal. This protects the piston
seals and pump heads by preventing a build-up of deposits from the
solvents.
1 Connect the tubing for the piston seal rinsing system. The
connections are simple plug-in fittings.
2 The three lengths of wide bore
tubing connect the pump heads
in series, connecting the top of
one pump head to the underside
of the next. The wide bore
tubing with the check valve
should be fitted between the
right pump head of pump
module A and the left pump
head of pump module B. The
arrow on the check valve must
point towards pump A.
3 Two lengths of narrow bore tubing are also supplied with the
pump. Connect the tubing with a Union Luer connector to the
underside of the left pump head on pump A. Connect the other
to the top of the right pump head of pump module B and
immerse the tubing in a flask containing rinsing solution (20%
ethanol).
4 Connect a syringe to the rinsing tubing that is connected to the
underside of the left pump head on pump A. Slowly suck up
rinsing solution to the syringe. When rinsing solution starts to
enter the syringe, continue to draw a few millilitres.
5 Loosen the syringe and immerse the tubing in the rinsing
solution.
Pump P-900
User Manual 18-1120-04 Edition AB
6
2 Installation
A B
P-901
P-903
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