AKTA design P-900 User manual

User Manual
18-1120-04
Pump P-900
18-1120-04_AB.qxd 1998-10-19 15:37 Sida 1 (1,1)

Trademarks
ÄKTA and UNICORN are trademarks of Amersham
Pharmacia Biotech Limited or its subsidiaries.
Amersham is a trademark of Nycomed Amersham plc.
Pharmacia and Drop Design are trademarks of
Pharmacia & Upjohn Inc.
Terms and Conditions of Sale
All goods and services are sold subject to the terms
and conditions of sale of the company within the
Amersham Pharmacia Biotech group which supplies
them. A copy of these terms and conditions of sale is
available on request.
Adresses
Amersham Pharmacia Biotech UK Limited
Amersham Place
Little Chalfont
Buckinghamshire
England HP7 9NA
Amersham Pharmacia Biotech AB
SE-751 84 Uppsala
Sweden
Amersham Pharmacia Biotech Inc
800 Centennial Avenue
PO Box 1327
Piscataway
NJ 08855 USA
© Amersham Pharmacia Biotech AB 1998
– All rights reserved
Important user information
Meaning: Consult the instruction manual to avoid
personal injury or damage to the product or other
equipment.
WARNING!
The Warning sign is used to call attention to the
necessity to follow an instruction in detail to avoid
personal injury. Be sure not to proceed until the
instructions are clearly understood and all stated
conditions are met.
CAUTION!
The Caution sign is used to call attention to
instructions or conditions that shall be followed to
avoid damage to the product or other equipment. Be
sure not to proceed until the instructions are clearly
understood and all stated conditions are met.
Note
The Note sign is used to indicate information
important for trouble-free or optimal use of the
product.
Should you have any comments on this instruction, we
will be pleased to receive them at:
Amersham Pharmacia Biotech AB
SE–751 84 Uppsala
Sweden
!
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Contents
1 Introduction
1.1 General ..................................................................................................................1
1.2 Safety.....................................................................................................................3
2 Installation
2.1 Unpacking..............................................................................................................4
2.2 General precautions ...............................................................................................4
2.3 Connecting electrical signal cables .........................................................................4
2.4 Connecting to communication link........................................................................5
2.5 Connecting to supply voltage.................................................................................5
2.6 Installing the piston seal rinsing system .................................................................6
2.7 Connecting the inlet tubing....................................................................................7
2.8 Purging the pump ..................................................................................................7
2.9 Running-in the new pump .....................................................................................8
2.10 Connecting the pump outlet tubing .......................................................................9
2.11 Installation of accessories.......................................................................................9
3 Operation
3.1 On/off..................................................................................................................11
3.2 Menu selection and settings .................................................................................11
3.3 Main menu overview ...........................................................................................12
3.4 Starting and stopping the pump...........................................................................13
3.5 Setting the flow rate and starting the pump.........................................................14
3.6 Setting concentration B........................................................................................14
3.7 Running a simple gradient ...................................................................................15
3.8 Ending the run and storage..................................................................................15
3.9 Changing eluent ..................................................................................................16
3.10 Restart after power failure...................................................................................16
4 Maintenance
4.1 Periodic maintenance ...........................................................................................17
4.2 Cleaning-in-place .................................................................................................17
4.3 General care.........................................................................................................17
4.4 Replacing the piston seal .....................................................................................18
4.5 Running-in a new piston seal...............................................................................22
4.6 Replacing a damaged piston ................................................................................22
4.7 Cleaning the inlet and outlet check valves ...........................................................23
Pump P-900
User Manual 18-1120-04 Edition AB i
Contents
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5Trouble shooting
5.1 General ................................................................................................................25
5.2 Faults and actions................................................................................................25
5.3 Error messages.....................................................................................................26
5.4 Checking the pump pressure................................................................................27
5.5 Removing trapped air bubbles from the pump ....................................................29
Reference information
A Description ..........................................................................................................30
A.1 Instrument.................................................................................................30
A.2 Pump head ................................................................................................31
A.3 Pump principle..........................................................................................34
A.4 Outlet manifold.........................................................................................35
A.5 Pressure monitoring ..................................................................................35
A.6 Using an external chart recorder ...............................................................35
B Menus..................................................................................................................36
B.1 Check menu ..............................................................................................36
B.1.1 Checking piston stroke .............................................................36
B.1.2 Check pump operation time .....................................................36
B.1.3 Check number of piston strokes ...............................................36
B.1.4 Check Service Mode .................................................................36
B.2 Setup menu ...............................................................................................37
B.2.1 Set pressure limit.......................................................................37
B.2.2 Set pressure full scale on recorder.............................................37
B.2.3 Set pressure unit........................................................................37
B.2.4 Set gradient base.......................................................................37
B.2.5 Set gradient mode .....................................................................37
B.2.6 Set pump type...........................................................................38
B.2.7 Set compression compensation..................................................38
B.2.8 Set mixer operation...................................................................39
B.2.9 Set valve operation....................................................................39
B.2.10 Setup language..........................................................................39
B.2.11 Setup unit number ....................................................................39
B.2.12 Setup display angle ...................................................................39
B.3 Alarm timer...............................................................................................40
B.4 Service displays .........................................................................................40
B.5 Menu overview .........................................................................................41
C Technical specifications .......................................................................................42
D Accessories and spare parts..................................................................................45
Short instructions on back page
About this manual
This manual comprises two parts; a practical part (sections 1 – 5)
and a reference part (sections A – D). Sections 1 – 5 contain the
necessary information for operating the instrument.
Pump P-900
User Manual 18-1120-04 Edition AB
ii
Contents
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1 Introduction
1.1 General
Pump P-900 is the collective name for a pump family. The family
members are:
• P-901 stand-alone or system mounted
Used as stand-alone, or in ÄKTA™ explorer 100. Equipped with
four pump heads in two pump modules.
• P-903 stand-alone or system mounted
Used as stand-alone, or in ÄKTA™ purifier 10/10 XT and
ÄKTAexplorer 10/10 XT. Equipped with four pump heads in two
pump modules.
Pump P-900
User Manual 18-1120-04 Edition AB 1
Introduction 1
Fluid inlet tubing
Out to system
P-900
P-901, P-903
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Pump P-900
User Manual 18-1120-04 Edition AB
2
All P-900 models are high performance laboratory pumps for use in
liquid chromatography and other applications where accurately
controlled liquid flow is required. Double mode can be selected to
double the flow rate range.
Pump P-900 features:
• P-901
- Pressure range 0 – 10 MPa (100 bar, 1450 psi).
- Flow rate range 0.01 – 100 ml/min isocratic and gradient mode.
- Double mode flow rate range of 0.01 – 200 ml/min isocratic
mode.
• P-903
- Pressure range 0 – 25 MPa (250 bar, 3625 psi).
- Flow rate range 0.001 – 10 ml/min isocratic and gradient mode.
- Double mode flow rate range of 0.001 – 20 ml/min isocratic
mode.
• The pumps are equipped with 2 pump modules, A and B,
including two pump heads each. This allows for binary gradients
with high pressure mixing.
• All pumps have a pressure sensor connected to pump module A.
• Low pulsation.
• Switch-valve control for low pressure gradients and BufferPrep.
All versions are run with the same software. Normal or double
mode, as well as the size of the pump heads are stated in a Setup
meny, see Reference information B.2.
Accessories
Accessories can be connected to the pumps, and can be controlled
from the pumps or from a PC running UNICORN™version. 2.10 or
higher.
These accessories are:
• Mixer M-925.
• 2-way Switching Valve SV-903.
1 Introduction
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1.2 Safety
•The instrument is designed for indoor use only.
• Do not use in a dusty atmosphere or close to spraying water.
• Do not block the air inlet and outlet of the unit.
Pump P-900
User Manual 18-1120-04 Edition AB 3
Introduction 1
WARNING! Always disconnect the power supply before
attempting to replace any item on the instrument.
WARNING! Incorrectly fitted tubing may loosen, causing a jet of
liquid to spray out. This is especially dangerous if hazardous
chemicals are in use. Connect the tubing by first inserting the
tubing fully, then tightening the connector fingertight. Finally
tighten the connector a further 1/4 turn using the key supplied.
WARNING! When using hazardous chemicals, all suitable
protective measures, such as protective glasses, must be taken.
WARNING! The instrument must not be opened by the user. It
contains high voltage circuits which can give a lethal electric shock.
WARNING! The instrument must be connected to a grounded
mains socket.
WARNING! NaOH is injurious to health. Avoid spillage.
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Pump P-900
User Manual 18-1120-04 Edition AB
4
2 Installation
2 Installation
2.1 Unpacking
Unpack the instrument and check the items against the supplied
packing list. Inspect the items for obvious damage which may have
occurred during transportation.
It is recommended that all packing materials should be retained if
onward transport of the instrument is expected.
2.2 General precautions
The instrument should be installed in a non-corrosive atmosphere.
The instrument should be located in a place of low temperature
variations, away from heat sources, draughts and direct sunlight.
The instrument may be operated at normal ambient temperatures in
the range +4 to +40 °C.
The instrument should be installed on a stable laboratory bench, in
ÄKTAexplorer or in ÄKTApurifier. To ensure correct ventilation a
free space of 0.1 m is required behind and in front of the instrument.
Place the instrument directly on the bench. Do not use any soft
material under the instrument, to ensure that the ventilation inlet in
the front is not blocked.
2.3 Connecting electrical signal cables
The sockets for electrical signals are located on the rear panel.
Valve 1
Valve A
Valve B
UniNet 2
Pressure,
analog
out 0 - 1V UniNet 1 Mains
Remote
CAUTION! The following information should be read carefully
to ensure that the instrument is installed correctly.
P-900
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Pump P-900
User Manual 18-1120-04 Edition AB 5
Installation 2
Connecting to chart recorder (if used)
1 Connect the chart recorder to the Mini-DIN-socket Pressure
using the cable supplied.
Pin Signal
1 Signal
2 Signal ground
3–6 Not used (Reserved for factory testing)
Note: The signal cable is delivered with protective covers on each wire.
Do not remove the protective covers from unused connections as a
short circuit may disturb the measurements.
2 Set the recorder to 0–1 V input, full scale.
3 Refer to section B.2.2 for a description of setting the full scale
pressure.
2.4 Connecting to communication link
The pumps used in ÄKTAexplorer and ÄKTApurifier, are controlled
from a PC running UNICORN version 2.10 or higher, using UniNet
cables.
1Connect two UniNet cables to the UniNet 1 connectors. The
instrument can be connected in series anywhere in the chain
between the PC and the termination plug. The UniNet 1 link
connects, in series, the PC with Pump P-900 and other
instruments. The termination plug is connected to the last
instrument in the chain.
2.5 Connecting to supply voltage
1Make sure the on/off switch is in the OFF-position O.
2 Connect a mains cable between the instrument and a grounded
mains socket. The instrument is delivered with both European
and US type mains cables, as standard. Any voltage from
100–240 V AC, 50–60 Hz can be used.
The instrument contains no user replaceable fuse.
CAUTION! The mains power to ÄKTAexplorer and ÄKTApurifier
must be switched OFF before connecting the instrument to the
UniNet 1 link.
WARNING! The instrument must be connected to a grounded
mains socket.
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2.6 Installing the piston seal rinsing system
Note: For the protection of the new pump, do not run the pump
until the system has been flushed out and primed with eluent,
according to sections 2.8 and 2.9.
The rinsing system for the piston seals must be installed before use.
20% ethanol should be passed around the piston and piston seal on
the non–pressure side of the piston seal. This protects the piston
seals and pump heads by preventing a build-up of deposits from the
solvents.
1 Connect the tubing for the piston seal rinsing system. The
connections are simple plug-in fittings.
2 The three lengths of wide bore
tubing connect the pump heads
in series, connecting the top of
one pump head to the underside
of the next. The wide bore
tubing with the check valve
should be fitted between the
right pump head of pump
module A and the left pump
head of pump module B. The
arrow on the check valve must
point towards pump A.
3 Two lengths of narrow bore tubing are also supplied with the
pump. Connect the tubing with a Union Luer connector to the
underside of the left pump head on pump A. Connect the other
to the top of the right pump head of pump module B and
immerse the tubing in a flask containing rinsing solution (20%
ethanol).
4 Connect a syringe to the rinsing tubing that is connected to the
underside of the left pump head on pump A. Slowly suck up
rinsing solution to the syringe. When rinsing solution starts to
enter the syringe, continue to draw a few millilitres.
5 Loosen the syringe and immerse the tubing in the rinsing
solution.
Pump P-900
User Manual 18-1120-04 Edition AB
6
2 Installation
A B
P-901
P-903
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2.7 Connecting the inlet tubing
The inlet tubing is connected to the centre
port of the inlet manifold of each pump
module.
Connect the tubing using male
5/16”-connections.
2.8 Purging the pump
1Fill a reservoir flask with distilled water. Immerse the inlet
tubings of both pump modules, with filters, in the water.
Note: Never place the reservoir flask below the level of the pump
inlet.
2 Connect a male Luer syringe of about 30
ml to the open end of the purge tubing.
3 Connect the male Luer connector at the
other end of the purge tubing to the left
purge valve at pump module module A.
4 Turn the purge valve counterclockwise
half a turn to open it and slowly draw
eluent to the syringe.
5 When fluid starts to enter the syringe
continue to draw a few millilitres before closing the purge valve.
Check that there is no air left in the inlet tubing.
6 Repeat steps 3 to 5 for pump module B, if fitted.
CAUTION! To protect the piston seals, the pump must never be
run with air in the inlet tubing. Follow the procedure below to
remove the air.
Pump P-900
User Manual 18-1120-04 Edition AB 7
Installation 2
Purge valve
Purge tubing
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2.9 Running-in the new pump
General
To be able to follow this section you must know how to operate the
pump. Read through section 3 Operation before continuing.
Note: To ensure trouble-free operation of the pump, it is important
that this running-in procedure is followed.
The pump heads are filled with 20% ethanol for protection during
transportation. This is removed by running-in the pump with a
liquid such as distilled water which is miscible with 20% ethanol.
After running-in, the pump is primed for the chromatographic run.
Note: If, for any reason, the pumpheads are dry, actions according
to section 4.5 Running in a new piston seal must be carried out
instead!
Running-in
1 Purge the pump, see section 2.8 Purging the pump.
2 Switch the mains power switch on the rear panel to ON.
3 Check that the outlet tubing are not blocked, and press OK in the
pump synchronization display.
4 Set the flow rate in menu Set Flow Rate. Run both pumps at the
same time by setting the concentration to 50% in menu
SetConcentrationB under menu SetConc./Gradient. Set the pump
in Run mode. Run the pump at the flow rates and times specified
below.
Pump Flow rate Time
P-901 40 ml/min 10 minutes
P-903 4 ml/min 10 minutes
5 Stop the flow by setting the pump in End mode.
Pump P-900
User Manual 18-1120-04 Edition AB
8
2 Installation
End 0.00ml/min
0.00MPa 0.0%B
Pump synchronization
Press OK to start
Selftest
Please wait...
Set Flow Rate
(0.00ml/min) 0.10
Run 0.10ml/min
0.00MPa 0.0%B
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2.10 Connecting the pump outlet tubing
1Remove the blind plug from the pump head outlet manifold.
Connect the outlet tubing using 1/16” Fingertight connectors.
Place the end of the tubing in a drain or waste collection vessel.
2 Close the purge valve by turning it clockwise.
3 Set the flow rate to 10
ml/min for P-901, and
to 1 ml/min for P-903.
4 Set the pump in Run
mode. Allow the pump
to run for 1–2 minutes
to prime the new outlet
tubing.
5 Stop the flow by setting
the pump in End mode.
2.11 Installation of accessories
Mixer M-925 and up to three 2-way Switching Valves SV-903 can
be connected to the pump:
Pump P-900
User Manual 18-1120-04 Edition AB 9
Installation 2
M-925
00,,66
Mixer M-925 Switch Valve SV-903
WARNING! Incorrectly fitted tubing may loosen, causing a jet of
liquid to spray out. This is especially dangerous if hazardous
chemicals are in use. Connect the tubing by first inserting the tubing
fully, then tightening the connector fingertight. Finally tighten the
connector a further 1/4 turn using the key supplied.
Purge valve
Outlet tubings
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Pump P-900
User Manual 18-1120-04 Edition AB
10
3 Operation
Mixer M-925 is connected to the UniNet-2 connector.
SV-903 valve for buffer selection is connected to Valve 1. The valve is
set on/off via the pump sub menu Set Valve 1 under the Setup menu.
See Reference information B.2.8.
SV-903 valves for BufferPrep are connected to Valve A and Valve B.
SV-903 for one-pump gradients is connected to Valve A.
Valve 1
Valve A
Valve B
UniNet 2
Pressure,
analog
out 0 - 1V UniNet 1 Mains
Remote
CAUTION! Before connecting any accessories, make sure the
power switch is in OFF position.
Fluid inlet tubing
Out to system
M-925
00,,66
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3 Operation
3.1 On/off
Switch on the instrument at the mains switch on the rear panel. At
switch on the instrument performs a selftest and then asks for
synchronization.
Open the purge valves (see section 2.8) to avoid high pressure during
synchronisation which is done at the speed of 0.1 ml/min, and press
OK. The display will show Synchronizing pumps and after approx. 1
minute the display shows the main operating menu with the pump in
End mode. Close the purge valves. All parameters are factory set to
default values.
3.2 Menu selection and settings
Menu selection
A specific menu is selected by turning the front selection dial
clockwise or counterclockwise. When the required menu is visible the
menu or selection is accepted by pressing the OK-button.
Menu selection OK-button ESC-button
Select submenu Return one menu level
Select sub-menu Return one menu level
If a menu has sub levels, the sub menu is displayed by pressing the
OK-button. Pressing the ESC-button moves back one menu level.
Pump P-900
User Manual 18-1120-04 Edition AB 11
Operation 3
Pump synchronization
Press OK to start
Selftest
Please wait...
End 0.00ml/min
0.00MPa 0.0%B
Pump P-900
V1.00
Main menu 1
Main menu 2
Main menu 3
Main menu 4
Main menu 5
Main menus Sub menus Sub menus
Sub menu 2.3.1
Sub menu 2.3.2
Sub menu 2.1
Sub menu 2.2
Sub menu 2.3
Sub menu 2 .1.1
ESC
OK
ESC
OK
ESC
OK
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Return to main menu
Pressing ESC repeatedly, always returns to the main menu 2 which is
the main operating menu. Press ESC once more to return to main
menu 1, the mode changing menu.
Select value
A cursor below a text or numerical value shows what is affected by
the dial. To increase the value turn the dial clockwise. To decrease
the value turn the dial counterclockwise. The value can be reset by
turning the dial several clicks counterclockwise.
When setting numerical values the cursor moves up to the next digit
if the dial is turned quickly in one direction, to simplify entering
large values. The cursor moves back one place to the right every two
seconds if the dial is not turned. The text or numerical value
displayed is accepted by pressing the OK-button. To cancel, press the
ESC-button.
3.3 Main menu overview
Main menu 1
Main menu 2 ESC ESC ESC ESC
ESC
Pump P-900
User Manual 18-1120-04 Edition AB
12
3 Operation
Set Hi Press Limit
(10.00MPa) 50.0
Parameter
Current value New value to be set
Set Conc./Gradient
(0.0%B)
Check
Alarm/Timer 12:30:52
Bzz12:33:00
Setup
Run 23.40ml/min
2.00MPa 45.5%B
Set Flow Rate
(0.00ml/min)
Main operating menu: The menu is accessed from all positions by
pressing the ESC-button repeatedly.
Setting flow rate in ml/min.
Setting concentration and gradient values.
Check internal operating values. See Reference information section
B.1.
Setup language, pressure limits, etc. See Reference information
section B.2.
Set different timer options. The pump can be started or stopped at
set times. See Reference information section B.3.
Mode changing menu. This is from where the pump is started and
stopped. The menu is accessed from all positions by pressing the
ESC-button repeatedly. The appearance of this menu will depend on
the current mode.
End 1.00ml/min
Run
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Pump P-900
User Manual 18-1120-04 Edition AB 13
Operation 3
3.4 Starting and stopping the pump
Main operating menu
The main operating menu shows the current flow rate together with
a mode indication, pressure and %B, if used. The available modes
are:
Run The pump is running with set flow rate.
End The pump is not running. Flow rate and gradient are
reset.
Pause The pump is stopped but the set flow rate value and
the gradient are retained.
Hold The gradient is held at the value displayed, and the
pump continues to run.
Mode changing menu
Mode changes are made in the mode changing menu above the main
operating menu (turn dial counter-clockwise). The current mode is
shown in the upper left corner of the display. Available actions are
shown at the lower right. There are four different displays for this
menu and the menu displayed will depend on the current mode.
When a new mode is selected the appearance of the menu will
change.
Pressing OK in a mode changing display will select the underlined
mode. Different modes can be underlined by turning the dial.
To start the pump: Select Run and press OK.
To stop the pump: Select End and press OK.
Preparation before starting
1 Check that there is sufficient eluent present for the run, and that
the solvent filter is fully immersed. If the eluent is to be changed,
see section 3.9 Changing eluent
Note: The pump may not work if the buffer vessels are sealed.
Do not close the vessels off completely.
CAUTION! Before the start of each run, always ensure that there
is an adequate supply of eluent in the reservoirs. Never allow the
pump to run dry, since this will affect the lifetime of the piston
seals.
WARNING! Incorrectly fitted tubing may loosen, causing a jet of
liquid to spray out. This is especially dangerous if hazardous
chemicals are in use. Connect the tubing by first inserting the tubing
fully, then tightening the connector fingertight. Finally tighten the
connector a further 1/4 turn using the key supplied.
Run 23.40ml/min
2.00MPa 45.5%B
Run 1.00ml/min
End Hold Pause
Current Mode Available
actions for
the current
mode
End 1.00ml/min
Run
Pause 1.00ml/min
End Hold Continue
Hold 1.00ml/min
End Continue Pause
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2If there is air in the tubing or the pump, prime the pump. Refer
to section 2.8 Purging the pump.
3 Check that there is sufficient liquid (20% ethanol) in the rinsing
system reservoir.
4 Set the pressure limit. Refer to section B.2.1 in Reference
information. If the pressure limit is exceeded, the pump is
stopped.
Emergency stop
By pressing ESC repeatedly and ending with OK, the pump is set to
Pause mode.
3.5 Setting the flow rate and starting the pump
1Select main menu Set Flow Rate, press OK.
2 Set the value, press OK.
3 Select the mode menu, select Run, press OK. The pump speed
gradually increases to deliver the set flow rate.
4 To change the flow rate while the pump is running, perform steps
1 and 2 again.
5 To stop the pump, select the mode menu, select End, press OK.
In UNICORN select instruction Flow in
System Control:Manual:Pump. Click on Execute and the pump will
start.
3.6 Setting concentration B
A percentage of eluent B can be set.
1 Select main menu Set Conc./Gradient, press OK.
2 The display shows Set Concentration B, press OK.
3 Set the %B value, press OK.
In UNICORN, select instruction Gradient in
System Control:Manual:Pump. Set length to zero.
Pump P-900
User Manual 18-1120-04 Edition AB
14
3 Operation
Set Conc./Gradient
(0.0%B)
Set Flow Rate
(0.00ml/min) 2.50
Set Concentration B
(0.0%B) 5.0
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3.7 Running a simple gradient
Gradients can be run in time or volume base. The default is time
base. To change base, see B.2.4 in section Reference information.
The gradient is run
from current
concentration to
target concentration,
in set length of time
or volume. The
gradient can be set
in any operating
mode.
1 Select main menu Set Conc./Gradient, press OK.
2 The display shows Set Concentration B. Turn the dial to select
menu Set Gradient Length, press OK.
3 Set the value, press OK.
4 Select menu Set GradientTarget. Set the gradient target value in %B,
press OK. If the pump is in Run mode the gradient will start
immediately.
5 At the end of the set gradient length time, the pump continues to
run at the target concentration.
In UNICORN, select instruction Gradient in
System Control:Manual:Pump.
3.8 Ending the run and storage
The pump can be left filled with a buffer overnight.
Note: If buffers or water are stored at room temperature, there is a
risk that bacterial growth may occur.
If no further runs are planned, the pump should be flushed
immediately with pure eluent. If aqueous buffers have been in use,
flushing with pure distilled water is particularly important to prevent
salt precipitation.
Overnight storage: The pump can be left filled with a buffer.
Weekend and Long time storage: Flush the pump with water and
then fill it with 20% ethanol.
Pump P-900
User Manual 18-1120-04 Edition AB 15
Operation 3
Set Conc./Gradient
(0.0%B)
0%
100% Target
concentration
Current
concentration
Gradient
started
Gradient length
Set Gradient Target
(0.0%B) 10.0
Set Gradient Length
(0.0min) 8.0
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3.9 Changing eluent
When changing from one eluent to another, it is extremely important
that the two eluents are totally miscible with one another. If the two
eluents are immiscible, the pump should be flushed first with an
intermediate liquid, which is miscible with both eluents. Failure to
do this will cause a wrong flow of eluent from the pump.
When changing from a salt-containing buffer to an organic solvent,
use water as the intermediate liquid to prevent precipitation.
1 Stop the pump by setting it in Pause mode.
2 Transfer the inlet tubing into the new eluent or into the
intermediate liquid.
3 Run the pump at a flow rate and time as specified in the table in
section 2.9.
4 Stop the pump. If an intermediate liquid is being used, transfer
the inlet tubing into the final eluent and repeat step 3 with the
new eluent.
In UNICORN, select instruction PumpWash in
System Control:Manual:Pump.
3.10 Restart after power failure
If the power supply to the instrument is interrupted, the instrument
automatically restarts itself doing selftest and prompting for
synchronisation. All values under the Setup menu are retained. Other
values, e.g. flow rate are reset.
CAUTION! To prevent precipitation of crystals when changing
from a salt-containing buffer to organic solvent, always flush
through the system with water as the intermediate liquid.
Pump P-900
User Manual 18-1120-04 Edition AB
16
3 Operation
18-1120-04_AB.qxd 1998-10-19 15:37 Sida 16 (1,1)