ARCTICO CRYO PORTER DP-80 User manual

OPERATING INSTRUCTIONS
DP-80 CRYO PORTER

1
Index
Index…………………………………………………………………………………… 1
Safety Precautions……………………………………………………………………. 2
Operator Limitation……………………………………………………………………. 7
Outline of the unit……………………………………………………………………… 8
Safety Function………………………………………………………………………... 9
Checkpoint before Using the unit…………………………………………………….. 9
Emergency Stop………………………………………………………………………. 9
Before Starting Operation…………………………………………………………….. 10
Operation………………………………………………………………………………. 11
Stop Operation………………………………………………………………………… 11
Temperature Settings/Controls……………………………………………………….. 12
Auto-tune………………………………………………………………………………. 13
Experimental Data…………………………………………………………………….. 14
Troubleshooting……………………………………………………………………….. 15
Specification…………………………………………………………………………… 17

2
Safety Precautions
ჀFor your own safety, please read through these safety precautions, prior to actual operation.
ჀThe following icons are the signs to avoid and prevent death, personal injury to others, or
damage to property. Read and follow these safety precautions carefully before use and use
this product accordingly. These precautions are classified as “DANGER”, “WARNING”, and
“CAUTION”, depending its level. As these signs are extremely important for your safety, follow
each instruction.
Risk Signs & Precaution
Risk of Death or Severe Injury, when used in wrong way,
and/or if precautions are ignored.
Risk of Serious Injury, when used in wrong way, and/or
if precautions are ignored.
Risk of Serious Injury, when used in wrong way, and/or
if precautions are ignored.
Icon Examples
These icons tell you the following actions are NOT allowed
and AVOID doing so. Specific actions you should take are also
mentioned nearb
y
.
These icons tell you the following actions are NOT allowed
and AVOID doing so. Specific actions you should take are also
mentioned nearb
y
.
(
Ex: “Do NOT disassemble.”
)
These icons are to instruct or enforce proper actions to take,
Or call your attention followed by the risk signs such as
“DANGER”, “WARNING”, or “CAUTION”. Specific actions
you should take are also mentioned nearby.
Disassembly
Prohibited
Prohibited
Careful for
Pinchin
g
Pull out
DANGER
WARNING
CAUTION

3
DANGER
Do NOT use the power cable except the one supplied with this product.
Danger of electric shock or short circuit causing fire, if any other power cable is used.
Use the proper power cable for the required voltage in the area/region.
Do NOT use the wrong fuse. Use proper fuse for the required voltage.
Using the wrong fuse can result in short-circuit causing fire. Initially installed fuse on
this unit is 0.4A fuse for 220-240V. DO NOT operate the unit at 100-120V without
changing the fuse. At 100-120V, the fuse has to be changed to the 0.75A fuse, which
is attached as the accessory.
Do NOT use a socket splitter or extension cable.
Voltage drop and the unit may stop working, or may cause fire.
If there is any unavoidable reason to use extension cable, enough electrical
capacity is required.
Do NOT touch or intend to operate this product with wet-hands.
There is danger of electric shock if you touch with wet-handed.
Do NOT operate with inappropriate voltage.
Wrong voltage may cause excessive heat of uncertain part of the unit, electric shock,
short circuit, or unpredictable fire. Check the operating voltage of the product and used
area before turning the power ON.
Do NOT damage the power cable.
Do not cut, damage, modify, sharply bend, twist or knot the power cable. Also, placing
heavy objects on the cable or forcing it into small spaces will damage the cable, which
may result in fire or electric shock
Keep the unit out of direct sunlight or immersing in rain and snow.
UV rays and excessive heat may cause damage to the unit. Operating in rain and/or
snow may cause danger of electric shock or risk of short circuit.
Do NOT insert any foreign objects/materials inside of the unit.
If any foreign object like water, metal items, or flammable items objects like paper is
brought in the air outlet vent, it may cause fire, electric shock, or malfunction. If any of
these objects is brought in the unit and cannot be taken out, immediately disconnect the
power plug and call for repair.
Do NOT disassemble, repair, or modify this unit.
This unit must not be disassembled, repaired, or modified by anyone except our repair
technician. Risk of combustion, electric shock, or malfunction causing injury. If any of
these incidents occurred by disassemble, repair, or modification without our permission,
responsibility of the result is at your own.
Prohibited
Disassembly
Prohibited

4
WARNING
Do NOT use the unit near any flammable items.
Keep flammables away from the unit. Do not operate the unit near the flammable
items such as gasoline, gas, thinner, or aerosol spray. Danger of explosion from these
flammables.
Do NOT use a socket splitter or extension cable.
Voltage drop and the unit may stop working, or may cause fire. If there is any
unavoidable reason to use extension cable, enough electrical capacity is required.
Do NOT use the unit on a surface tilted or with the side-surfaced up.
Operated in these position is prohibited. It may result in dropping the unit, and/or
malfunction.
Do NOT touch the container (i.e.: Aluminum Block)/parts when it is frozen to
ultra-low temperature range.
Do not touch the part/area concerned to be frozen (such as Aluminum Block, or other
container) directly with bare-hands. Risk of frostbite and/or serious burns. When it is
necessary to touch these part/area, do it so only after the part/area is completely
defrosted, or make sure to use the dry cloth when it is necessary to do so before
defrosted. Putting on the rubber gloves is the most recommended for your own safety.
Do NOT insert any food or animal inside of the unit.
This unit is for the purpose of keeping samples/objects in tubes or schales, not for
keeping foods or animals. To insert foods in any place of the unit and/or animals are
strictly prohibited.
Be careful for your hands/fingers not to be pinched when closing the lid.
Gently close the lid, to avoid pinching your hands/fingers. Attention required to prevent
pinching your hands/fingers while the lid is open.
Properly connect power cable and plug outlet.
Loose connection may cause fire at connected parts, voltage drop leads to operational
abnormality, or unexpected harmful accidents.
Always check and confirm to use the proper cable for the required voltage prior
to operate the unit.
Using wrong power cable at the wrong voltage may cause fire, voltage drop leads to
operational abnormality, or unexpected harmful accidents.
Keep the unit out of humidity and/or dusts.
If inside of the unit is dust-covered especially at high humidity, it may cause damage the
parts, short circuit, or unpredictable fire.
(Humidity: Max. 85%, without condensation.)
Careful for
Pinching
Prohibited
Attention

5
Take enough provisions before setting the unit.
Place the unit on flat, solid, stable, and spacious surface. If the area to set the unit is
narrow, the unit may be dropped off and causes unpredictable accidents. If used in a
vehicle, take enough provisions to prevent dropping off from the set place when any
sudden stop/curve happened. Dropping may cause malfunction.
Defrost the Aluminum Block, after turning the power OFF.
Defrost periodically, for better temperature accuracy. To defrost the Aluminum Block,
turn the power OFF, wait until it is completely defrost and wipe the frost/moisture away
with soft cloths. If it is in a hurry, take the block out with the rubber-gloves or soft cloths,
and soak it in lukewarm water.
In case if any abnormality is noticed;
If any abnormal noise, smell, and/or smoke is occurred/noticed, stop operation,
immediately disconnect the power plug and call us for repair.
Place the unit at well-ventilated, cool environment.
Do not block the air vent with any disturbed object. Operating the unit at ill-ventilated
and narrow area, or with anti-dust object covered on, may overheat the inside of the unit
and/or may cause insufficiency of the exhaustion, occurring overheat the whole unit,
unpredictable fire, accidents, malfunction.
20cm
20cm20cm UNIT
Attention

6
CAUTION
Do NOT place the unit on high places when you store the unit.
When you store the unit, avoid placing on high places, like top of the drawer or cabinet.
It may risk dropping of the unit.
Avoid long-term preservations of irreplaceable samples/objects.
Take extra attention for preservations of the samples/objects. This unit is designed for
temporary storage, preparative freezing, or short-distance delivery (by person or by
vehicle), as it is not integrated with back-up/save system. Take enough provision for
the case if the unit is failed its working by any incidents like sudden black-out or short
circuit.
Do NOT store the unit in a vehicle.
Keeping the unit in a vehicle may damage the surface, parts, and/or electrical area of
the unit by the room temperature change.
Handle the power cable gently.
When removing the power plug, be sure to hold the end of the plug rather than pulling
on the power cable. Check the power cable periodically. When any external damage
is noticeable, repair or change the cable promptly.
Call for check-up or repair when the unit is dropped.
When the unit is dropped down from the surface to the floor, stop operation immediately,
disconnect the plug, and call for check-up or repair.
Avoid operating in places under excessive heat.
Temperature of contents may rise, causing damage or malfunction.
(Recommended Room Temperature: 25ഒ)
*For operating in a vehicle, set the room temperature to 25ഒprior to the actual operation.
Do NOT turn the power ON/OFF consecutively.
After turning OFF (including when it is automatically turned off), take at least 30 seconds
for another turning ON. Turning the power ON/OFF consecutively causes malfunction.
Disconnect the power plug, when you store the unit.
When you do not use the unit, clean both exterior and interior, cover the unit for prevent
dust or foreign objects/materials got in from the air outlet vent, and store it to the place
with low humidity.
If used in a vehicle, disconnect the DC power cable whenever the vehicle’s
engine is turned off for extended periods of time.
In some cars, the power does not turn off automatically after switching off the vehicle’s
engine. Always make sure to turn off the power of the unit after turning off the vehicle’s
engine.
To store, disconnect the power plug.
For your safety, disconnect the power plug when not using the unit over 14days or
more.
Prohibited
Attention
Pull out

7
Operator Limitation
The following persons are NOT allowed to operate this unit.
The person who:
- Has/can not read this operation manual thoroughly.
- Is a child under 12 years old.
- Has total or partial blindness.
- Has a sense deficiency of sense of temperature or sense of touch.
- Is a psychiatric patient.
- Is under the influence of alcohol.
- Is under the influence of a controlled substance such as opium, morphine, cocaine, etc.
- Has a heart pacemaker.
- Is not wearing appropriate safety gears, such as rubber gloves.
- Has not had his/her long hair in a know to prevent from being caught to the unit.

8
Outline of the unit
This unit is Cryogenic Refrigerator “Cryoporter” (Model: DP-80 ), designed and produced for
the purpose of keep the samples/objects at the temperature range between 0ഒto –80ഒat the
laboratory. Do NOT use this unit for any other purpose.
This unit is portable, it is able to keep the desired temperature when it is operated with the car
battery using DC12V cigarette lighter plug. However, when used this unit in a vehicle, it is
necessary to pay extra attention on driving the vehicle. For your safety, and in order to use the
unit with maximizing its operational ability, carefully set the unit as instructed on this operation
manual.
཰Handle ུMain Power
For carrying the unit. For Turning ON/OFF.
ཱLid Latch & Operating Button ཱུFuse Holder
Push the button and pull up the lid. For Anti-Overcurrent
ིLid ྲྀDC12V Power Socket
Close while operating. Plug in the cigarette lighter cable for the use in a vehicle.
ཱིTemperature Controlling Panel ཷAC100ᨺ120V Power Socket
Indicates/controls the current/set temperature. Plug in the AC cable.
Caution: Do NOT use AC Power Cable & DC Power Cable at the same time.
It may cause risk of short circuit or electric shock.
䐟䐠
䐡
䐢
յ
ն
շ
ո

9
Safety Function
If abnormal temperature rising occurs during operation in the storage compartment or in the en-
automatically. (Main power shall still remain “ON” until it is turned off.)
In case the unit shows this condition;
*Turn OFF the main power immediately
*Check the unit if there is any blocking objects especially around the air ventilation area behind
the unit. (See page 5; “Place the unit at well-ventilated, cool environment” section.)
After checking and removing any blocking objects, turn ON the main power. If the unit still
shows the same condition, turn OFF the main power and call for repair.
Checkpoint before Using the unit
For your own safety, check the followings before using the unit every time when it is used;
-Any crack on the unit?
-Any crack, damage, tear on the power cable?
-Any abnormal noise when turning the main power ON?
If any of these abnormalities is noticed, do not use or immediately turn off the unit, and call for
repair.
In Case of Emergency
Turn the main power switch (on the left side of the unit) OFF, in case of emergency. In case if
any abnormality such as abnormal noise, smell, and/or smoke, etc. is occurred/noticed, stop
operation and immediately disconnect the power plug and call for repair.
gine room, a buzzer alarm (acoustic alarm) will be turned on, and the COOLING WILL BE STOPPED

10
Before Starting Operation
1. Setting Aluminum Block
2. Setting Container (Tubes/Receptacles)
1) Prior to setting this unit, check all optional parts are ready to use at your side.
2) Insert the optional “ALB (Aluminum Block) Holding Spacer” into the cooling space before
setting Aluminum Block. (“ALB Holding Spacer” is initially equipped at the cooling space.)
NOTE:
Also check there is no any blocking object like paper trash. If there is any blocking Item
between Aluminum Block and the cooling space, it may cause difficulties to reach to the
desired temperature.
1) Insert the tube/receptacles to the wells of Aluminum Block.
2) After inserting the tubes/receptacles, place the optional “Blocking Lid” on top of Aluminum
Block. (“Blocking Lid” is already equipped on top of Aluminum Block at your purchase of
the unit.)
NOTE:
This “Blocking Lid” is for the purpose of blocking absorbing the humidity from the outside of
the unit. It is not necessary, but to prevent frosting, we strongly recommend you to place it
on top of Aluminum Block while the unit is operating.

11
Operation
1. When used indoors
2. When used in a vehicle, at DC12V
Stop Operation
1) Check the main power on the left side of the unit is OFF, before starting the unit.
2) Insert the AC power plug into the AC power socket of the unit.
3) Insert the power plug to the household power supply provided, as far as it will go.
4) Turn ON the main power.
1) Set the unit on a flat, solid surface with the lid facing up. Do NOT use the unit on a surface
tilted, as it may result in malfunction.
2) Insert the power plug to the unit.
3) Insert the power plug into the cigarette lighter or accessory socket.
(The unit immediately starts when the power plug and the socket is connected.)
4) Connect the plug and the socket and use the unit only when the vehicle’s engine is ON.
NOTE:
Do NOT connect the power plug into AC plug of the unit. It may consume too much
batteries of the vehicle.
1) Turn the main power OFF.
2) To disconnect the power plug is recommended, to avoid unexpected fire or accident.

12
Example: When setting at –50Υ(Initial setting temperature is at –80Υ.)
1) When the main power turned ON, the display shows the
initial setting temperature at each line of “PV” (Processing Value)
and “SV”(Set Value).
[Example shows the present temperature of Aluminum Block
as 25䉝, setting(desired) temperature as –80䉝.]
2) Press “MODE” key once, for changing the setting temperature.
Press 䕦/䕰(Up/Down key) for the desired temperature.
Pressing 䕦/䕰(Up/Down key) once makes the setting temperature
Bring up/down 0.1䉝. For more faster/wider change of the
Setting temperature, keep pressing 䕦/䕰(Up/Down key) for a while.
3) Press “MODE” key 3(three) times, for back to the initial set-up
display, and the temperature setting is done. It starts the operation
comes to –50䉝(setting temperature).
ձ
ղ
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Temperature Settings / Controls
཰PV: Processing Value ཱིMODE: For Various Settings
ཱSV: Set Value ུႭ(DOWN): For lower temperature
ིStatus: ཱུႣ(UP): For higher temperature
MV: Monitoring Value
AT: Autotune is ON
Basic Tip:
Pressing key moves to the next stage.
NOTE: “Optional Mode 1&2” are preset at –99.9ഒat your purchase of the unit. Do not
change the temperature.
25.0
-80.0
-50.0
SET
25.0
-50.0
MODE
PV
SV
25.0
-50.0
PV PV PV
25.0
SET
-99.9A1AL -99.9A2AL
SV SV SV
䉝 䉝 䉝 䉝
Operating
(Initial Display)
Temperature Setting
(Desired Set Temp.)
Optional Mode 1
(Not Necessary for
Operating the unit.)
Optional Mode 2
(Not Necessary for
Operating the unit.)

13
Setting Auto-tune mode:
1) After setting the temperature following on the previous page,
press “MODE” key once, for setting Auto-tune Mode.
2) Press “MODE” key longer than 3(three) seconds.
Lower side of the display shows “LOC”. At the same time,
Upper side of the display shows “OFF”. (If it shows “ON”,
Press 䕦/䕰(Up/Down key) to choose “OFF”. It is preset as
“OFF” initially.)
3) Press “MODE” key once.
Upper side of the display shows “ONOF”.
Press 䕦/䕰(Up/Down key) until “PID” is shown on
the upper side of the display.
At the same time, Lower side of the display shows “CTR”.
Press 䕦/䕰(Up/Down key) until “OTDB” is shown on
the lower side of the display.
4) Press “MODE” key once.
Upper side of the display shows “ON “. At the same time,
Lower side of the display shows “AT UN”. If the upper side of
The display shows “OFF”, press 䕦/䕰(Up/Down key to choose
“ON”. It is preset as “OFF”in
itially.)
5) Press “MODE” key once.
“AT”(Auto-tune) is shown on the lower side. Now Auto-tune Mode
is set and automatically controls the temperature.
NOTE:
When Auto-tune is finished, “AT” display vanishes.
When Auto-tune temperature is set at near the room temperature,
it may take long time to finish or cannot be set. In these cases,
re-try setting Auto-tune Mode by setting upper/lower temperature.
When canceling Auto-tune Mode:
1) Press “MODE” key once.
2
)
To
g
o back to the initial dis
p
la
y
,
p
ress “MODE” ke
y
3
(
three
)
times.
Auto-tune
After setting the desired temperature, the unit shall be operated with temperature-controlled.
For more accurate temperature controlled operation, setting “PID Auto-tune Mode” is
recommended.
After setting “PID Auto-tune Mode”, it shall be set PID automatically revise the inequality of the
temperature. Unless you prefer to change the setting temperature, it is unnecessary to make
auto-tune again.
-50.0
SET
OFF
LOC
PID
CTR
ON
AT U N
-50.0
AT -50.00

14
Experimental Data
1㸧Cooling Speed with 1.5ml Tubes(Water:1350ul)
2㸧Cooling Data of various sized tubes
3㸧Temperature Keeping Ability with 1.5ml Tubes (Water:1350ul)
1350ul
450ul
150ul
50ul
䈜Inserted samples at 䠉80䉝.
Measured Time
Temperature( 䉝 )
Tem
p
erature
(
䉝
)
Sample Tube’s Temperature
䈜After turning the Power OFF at –80䉝.
Measured Time
Measured Time(h:m:s)
Temperature( 䉝 )
Inside Temperature of the unit
䊼
䊺
Sur
f
axe Tem
p
o
f
Aluminum
䈜Inserted samples at turning on the unit.
Sample’s
Tem
p
erature

15
Disassembly
Prohibited
Troubleshooting
WARNING
Do NOT disassemble any parts screwed up, or open the back panel.
These places/parts must not be disassembled/opened, by anyone except our repair
technician. Risk of combustion, shock, or malfunction causing injury.
If any of these incidents occurred by disassemble, repair, or modification at your side
without our supervision/permission, we cannot take responsibilities.
For any necessary repair, contact us/our dealers ONLY.
Do not tend to repair this unit at your own or any other person. Risk of combustion,
shock, or unpredictable fire.
When the followings are conformed at returning or asking for repair this unit or
any parts of this unit, sterilize the unit completely before dispatch the unit..
1) When it is considered any contagious/radioactive substances might have
contaminated whole unit or any part of the unit.
2) When it is considered there is any danger/risk when blood or any
biological/chemical substances are adhered to whole unit or any part of the unit.
These cases are extremely dangerous for causing secondary
contamination/contagion. Inform us, or our dealer prior to send the unit for
repairs.
ჀPlease check the following points before contacting us for repairs.
ჀIf you are not sure about the cause of the trouble, disconnect the power plug and attach the
note describes the problem, and contact us or the our dealers you have purchased the unit.
Problems Possible Cause Solution
Unit does not start
operation after turning the
power on.
ON/OFF button has not been
pressed continuously for
1(one) second.
Push the button until you hear a high-pitched
tone (approximately 1 second.)
Electrical connections are not
properly connected.
Check electrical connections, such as Power
plug, socket, or power supply.
Ashes or dust in the power
supply socket.
Clean the power supply socket.
Fuse has blown. After turning the power OFF, replace the fuse
in the fuse-box. If the same problem
occurred after replacing the fuse, contact us,
or our dealers near you.
Power button is broken. Contact us, or our dealers.
Attention

16
Unit does not cool
sufficiently, or takes too
long to reaching the
desired temperature
(longer than 90 minutes.)
Environmental temperature is
considered to be too high.
Set/change and keep the room temperature
approximately 25ഒ.
The lid is not completely
closed.
Close the lid properly and lock it.
The air outlet vent is covered
or disturbed.
Check the air outlet vent on the back.
Remove any items covering the vent.
PV/SV Indicator displays
incorrectly.
Electro-magnetic interference
may near the unit.
Uncontrollability of CPU in the
controlling unit occurred by
these devices.
1) Leave the unit away from these devices.
2) Turn OFF, leave it for about 10 seconds
and turn the power ON again.
If the incorrect display continues, contact
us or our dealer you purchased unit.

17
Specification
Temperature Range Approximately –80ഒᨺ0ഒ
Temperature
Accuracy s0.2ഒ
Temperature
Controlling System PT100/Digital PID Indication, Inverter Control
Cooling System Free Piston Sterling Cooler, 40W, supplied by Twinbird Corporation
Fuse AC220-240V, 0.4A /DC12V, 8A
Power 62VA/AC100 – 69VA/AC115 –82VA/AC240V – 60VA/DC12V
Dimension W230(275, including the handle)mm x D262mm x H390 mm
Weight Approximately 10kgs, without Aluminum Block
Accessories
1) AC Power Cable for AC220–240V x 1
2) DC Power Cable for DC12V x 1
3) ALB Holding Spacer (For Holding Aluminum Block) x 1
4) Blocking Lid (For Anti-defrosting, Keeping temperature) x 1
5) 8A Fuse for DC12V (large) x 1, in the DC Power Plug
6) 0.75A Fuse for AC100-120V x 1
(For the use at 100-120V ONLY. DO NOT use this fuse at 220-240V.)
Attached
Documents
Operation Manual x 1
Warranty x 1, Attached to Operation Manual
Optional Aluminum
Blocks’ specification
(Sold Separately)
Type Spec/Well
DPB-0296 0.2ml x 96-well (For 0.2ml PCR Tubes)
DPB-0548 0.5ml x 48-well
DPB-1840 1.8ml x 40-well
DPB-2040 2.0ml x 40-well
Other specs or types are available upon request (Price may vary). Ask
our dealer for more information.
DPB-1548 1.5ml x 48-well

18

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