biochrom ZENYTH 200 User manual

March 11 Zenyth 200 Quick Start Guide Version 2.0
BIOCHROM ZENYTH 200 MICROPLATE READER
QUICK START GUIDE
1. Turn on the instrument:
Connect instrument to a power source using the appropriate power cord.
Check user’s manual for important safety information.
2. Connect the instrument to a PC:
Connect to a PC via serial port to serial port or a serial port to USB port adaptor. To
determine the communication port (com) used by the instrument, and open the device
manager, found here: go to Start\Control Panel\System\Hardware\Device Manager\Ports.
Please Note:
Ensure that the instrument is connected using COM ports 1 –9.
Some USB to RS232 converters do not work well; use a serial port whenever
possible.
Ensure that you are using the original RS232 cable that was shipped with the
instrument.
3. Connect instrument to ADAP software:
Insert CD supplied with the instrument into PC; install ADAP. Open ADAP. ADAP will prompt
for a user ID and password. Use the pre-set ID and password: sadmin\sadmin. Once logged
as sadmin, set specific user IDs, passwords and administrative rights. Select
Setup>Instrument in the menu bar. A dialogue box will open:
Under the Instrument tab:
In Baudrate: select Auto Sense
In COM Port: select port
In Instrument Type: select Zenyth200

March 11 Zenyth 200 Quick Start Guide Version 2.0
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To confirm that the instrument is connected with the computer, select the Read
Configuration button. The serial number of the instrument should now appear in the
Setup>Instrument dialogue box along with compatible plate types in the Installed Plates
window.
4. For a quick measurement, select in the task bar or Reading>Quick:
In the Quick-Read dialogue box:
Confirm that the correct format and
plate type are selected.
Select All in Measurement
Position to read the entire
plate.
Select Endpoint Photometric
for basic readings using a
measurement and reference
filter.
Note: It is important to use a reference filter to account for optical inference from
the plate.
5. Place plate with A1 in the upper left corner of the plate transport. Select Start. Absorbance
measurements will appear in the open window in ADAP.
6. Other options in the Quick-Read dialogue box include: Kinetics measurements and multi-
wavelength measurements.
7. To export raw data, select the Raw Data tab. To export the data as a matrix, select Copy
displayed data into clipboard. Data will paste as a matrix with filter wavelength, time and
date. To export multiwavelength or kinetic measurements, select Copy all data into
clipboard. Data will paste as a matrix showing the measurement at each wavelength or time
of measurement grouped by well.