CHART MVE Fusion User manual

20994124 REV A  1
MVE FUSION
Technical Manual

20994124 REV A  2
MVE FUSION
Technical Manual
PN 20994124 Rev A
1 Preface
Chart Inc.
2200 Airport Industrial Drive, Suite 500
Ball Ground, GA 30107 USA
Customer / Technical Service:
The Americas Phone: [phone] Fax: [phone]
[phone] [phone]
Email: [e-mail]
Asia, Australia, Pacific Rim Phone: [phone] Fax: [phone]
Europe Phone: [phone] Fax: [phone]
This manual covers the use and maintenance of MVE Fusion Freezers. READ BEFORE USING THIS EQUIPMENT.
Failure to follow the instructions in this manual can result in damage to the unit, injury to personnel, and/or poor
equipment performance. It is intended for use by trained personnel only. All service and maintenance should be
performed by an authorized MVE Distributor or Service Technician.
NOTE: All MVE Fusion models are Class I per IEC 61140, as the AC electrical supply requires a protective earth
ground. These devices are externally powered and intended for continuous operation. The models are
intended for professional use in research, while the medical device versions are intended to be used in
situations that directly support medical applications. They are not suitable for use with flammable anesthetics.
These devices have been tested to, and are in compliance with UL 61010-1:2012 Ed.3 & CSA
C22.2#61010-1:2012 Ed.3 +G1.

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This manual includes the following symbols.
Table 1: the symbols and their descriptions
Symbol Title Description
Caution
Signifies a CAUTION of potentially hazardous
situation when operating the device that may
result in minor to moderate injury or property
damage.
Warning
Signifies a WARNING of a potentially hazardous
situation when operating the device that may
result in serious injury or property damage.
Warning; Low
Temperature
Indicates low temperature or freezing conditions.
Take care to avoid exposure to skin, eyes, and
clothing.
Warning;
Asphyxiating
Atmosphere
Indicates the potential for an oxygen-depleted
atmosphere due to nitrogen vapor. Take care to
operate device in a well-ventilated area.
Warning; Electricity Indicates a potential electrical hazard. Take care
to avoid contact with electricity.
Warning; Explosive
Indicates a potential explosive hazard. The
expansion ratio of liquid nitrogen to gas is 1:700
and can cause explosive conditions if placed into
a sealed container.
Wear Protective
Gloves
Thermal gloves must be worn during indicated
procedures.
Wear a Face Shield A face shield must be worn during indicated
procedures.
ETL Listed Mark Chart Fusion Freezer conforms to UL STD 61010-
1 and certified to CSA STD C22.2#61010-1.

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2 Safety
READ BEFORE OPERATING THIS EQUIPMENT
General
Liquid nitrogen (LN2) is used in MVE Fusion Freezers as a refrigerant. Understanding
and following certain safety precautions is extremely important when handling LN2 and
cryogenic containers (commonly referred to as dewars).
Liquid Nitrogen Properties
Nitrogen is a colorless, odorless, tasteless gas. Gaseous nitrogen makes up about 78%
of the Earth’s atmosphere by volume. Once collected, cooled and isolated, nitrogen can
be liquefied.
Boiling Point at 1 atmosphere -195.8°C , -320.3°F , 77.4 K
Thermal Conductivity (Gas) 25.83 mW/(m·K)
Heat of Vaporization (Liquid) 198.38 kJ/kg
Liquid density at 1 atmosphere 1.782 lbs/L , 807.4 g/L , 808.6 kg/m3
Liquid Nitrogen Safety
Transferring LN2 and operating the MVE Fusion should be done in accordance with the
manufacturer/supplier instructions. It is important that all safety precautions
recommended by the manufacturer be followed.
 WARNING: Do not modify this equipment without authorization
of the manufacturer.
 Nitrogen vapor is a potential asphyxiant as it displaces Oxygen
(O2) in confined spaces. Rapid suffocation can occur without
warning in an Oxygen-deficient atmosphere (less than 19.5%

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O2). Chart Cryogenic Freezers must be installed and operated
in well-ventilated areas.
 DO NOT vent container in confined spaces.
 DO NOT enter confined spaces where excess nitrogen gas may
be present.
 If exposure has occurred move to ventilated area or fresh air. If
breathing is difficult, supplement oxygen may be required. If
not breathing, give artificial respiration. SEEK IMMEDIATE
MEDICAL ATTENTION.
 Contact with liquid nitrogen or uninsulated equipment
containing nitrogen can result in cold contact burns or tissue
damage. Nitrogen vapor can cause damage to skin or eyes.
 In case of frostbite, warm area with warm water not exceeding
105°F (40°C) and SEEK IMMEDIATE MEDICAL ATTENTION.
 Never place LN2 in a sealed container without a pressure relief
device. The expansion ratio of liquid nitrogen to gaseous
nitrogen is 1 to 700 (1 cubic foot of liquid nitrogen becomes 700
cubic feet of gaseous nitrogen when evaporated).
The two most important safety aspects to consider when handling LN2 are adequate
ventilation and eye and skin protection. Although nitrogen gas is non-toxic, it is dangerous
in that the gas will displace oxygen in a normal breathing atmosphere. Liquid products
are of even greater threat since a small amount of liquid evaporates into a large amount
of gas. Therefore, it is imperative that cryogenic supply and storage dewars be stored
and operated in well-ventilated areas.

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Persons transferring LN2 should make every effort to protect the eyes and skin from
accidental contact with liquid or cold vapor. Chart MVE recommends the following
protective clothing and accessories when transferring LN2 or handling hoses, valves, and
plumbing components:
 Cryogenic gloves (loose fitting)
 Full-face shield or chemical splash goggles
 Cryogenic apron
 Long sleeve shirt and cuffless pants
 Closed toe shoes (no sandals)
Equipment Usage
Cryogenic containers must be operated in accordance with the manufacturer/supplier
instructions. Cryogenic dewars must be kept in a well-ventilated area protected from
weather and away from heat sources. In applications that use a modular liquid cylinder
as a source of LN2, the supply will need to be replenished at regular intervals to ensure
proper operation of the freezer.
Recommended First Aid
Every site that stores and uses LN2 should have an appropriate Material Safety Data
Sheet (MSDS) present. The MSDS may be obtained from the manufacturer/distributor.
The MSDS will specify the symptoms of overexposure and first aid to be used. Here is a
typical summary. If symptoms of asphyxia such as headache, drowsiness, dizziness,
excitation, excess salivation, vomiting, or unconsciousness are observed, remove to fresh
air. If breathing has stopped, give artificial respiration. CALL A PHSYICIAN
IMMEDIATELY.
If breathing is difficult, supplemental oxygen maybe required. If exposure to cryogenic
liquids or cold vapor occurs, restore tissue to normal, body temperature (37°C) as rapidly
as possible, and then protect the injured tissue from further damage and infection.
Rapid warming of the affected areas is best achieved by bathing it in warm water. The
temperature of the water used should not exceed 40°C. Under no circumstances should
the frozen part be rubbed either before or after warming. If the eyes are involved, flush
them thoroughly with warm water for at least 15 minutes. In case of massive exposure,
remove clothing while showering with warm water. The patient should not drink alcohol
or smoke. CALL A PHYSICIAN IMMEDIATELY.

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3 System Components and Function
3.1 System Overview
The MVE Fusion system consists of several key components identified in Figures 1 and
2: dewar, Pressure Vessel, Liquefier, Shroud, Graphical User Interface (GUI), and
Retractable Stairs.
Figure 1: MVE FUSION FREEZER

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Figure 2: Cross Section of the MVE Fusion Freezer

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3.2 Liquefier
The Liquefier contains the cryocooler and other support / interface control systems
required to operate the MVE Fusion Freezer. Replacement of the liquefier should only be
performed by a representative from Chart Inc. or trained authorized personnel.
The Liquefier consists of the following major components and features:
 Cryocooler – This is the heart of the system that maintains the liquid nitrogen
stored within the systems pressure vessel. See page 12 for more details pertaining
to the Fusions cryocooler.
 Graphical User Interface (GUI) – This is a touch-screen interface with customized
software intuitively designed for monitoring the MVE Freezer. The 155mm x 87mm
backlit LED display provides system security through password protected
admission. The main screen allows quick access to and displays key parameters
such as the internal freezer temperature, the cryogen liquid level, cryocooler motor
temperatures, the cryogen pressure and system alarms.
 Battery Backup – A 12V, 9.0Ah, sealed lead acid battery allows for monitoring of
the freezer parameters and alarms for a minimum of 24 hours after a power loss.
 Main Control Board – An advanced printed circuited board (PCB) with control
logic and memory for storage of settings, parameters and historical events.
 Variable Frequency Drive (VFD) – The cryocooler is powered by this variable
frequency, variable voltage power supply. Control of the VFD is accomplished by
signals sent from the main PCB based on the freezer status. Voltage to the
cryocooler is modulated to maintain a constant freezer temperature and optimize
power consumption.

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 Cooling Fans – Two 12 VDC, 150CFM fans with “CPU” style heat exchangers
reject heat from the cryocooler and electrical components within the liquefier to the
ambient environment.
 Alarms – In addition to the visual alarms displayed on the GUI, an alarm speaker
is provided that sounds when any preset threshold (freezer temperature, low
battery, motor temperature, VFD temperature, gas pressure, etc.) is exceeded or
if the freezer lid has been removed for more than 5 minutes.
 Serial Communication – Two full-duplex RS-232 communication ports are
provided to monitor the VFD and control system and modify parameters. These
are for use by trained service technicians or other qualified personnel only.
 USB port – A standard USB interface allows easy access to download the event
historical logs.
 Global Alarm Contacts – A normally open and normally closed set of dry contacts
are provided for monitoring alarm conditions.
Figure 3: Front Perspective of Liquefier with the Cowling off