CorSolutions PeriMicro User manual

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CorSolutions Version V2.003
The PeriWave Pump Product Line:
PeriMicro, PeriMilli, and PeriNano OPERATOR’S MANUAL
Congratulations on your purchase from CorSolutions! Please read this Operator’s Manual which explains
proper operation of the fluid delivery pump.
Safety Warning: - Not approved or intended for human use.
- Always wear protective eyewear when pump is in use.
Liquid Outlet
Liquid Inlet
Pump
Rollers
Flow
Sensor

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CorSolutions Version V2.003
SETTING UP THE PUMP
Hardware and Cable Connections. The pump has been shipped with a Power Supply and appropriate
interface cables. The power supply and USB cable connect to the receptacles located on the rear panel of the
pump. The power supply connects to a 110-220V AC power source. The USB cable should be connected into
the host computer USB port. The hardware and cable connection set-up is now complete. The power switch
is also located on the rear panel of the pump.
If you are using two pumps with one computer, repeat the process.
IMPORTANT POINTS:
1) Pump is designed and calibrated for aqueous-based solutions. If other solution types are used,
the flow rates will not be accurate.
2) For proper operation, 1/32-inch tubing must be attached to the flow-sensor outlet. This tubing
is part of the dampening system and is needed for proper closed loop feedback for the flow
control. If a different outlet tubing type is desired, please employ a union at the end of the
1/32-inch outlet tube to connect to the desired tubing type. CorSolutions has adapters
available to convert from 1/32-inch to 1/16-inch (part number: Union-188-P)
3) The lines should be rinsed with 5% bleach before turning off the pump or after use with salty
or viscous solutions. This will ensure the flow sensor is maintained and will prevent it from
clogging and avoid the growth of bacteria.
4) Only use water-based solutions or 20/80 ration of Isopropynol/Water to rinse the lines. Use of
solvents such as acetone and Ethanol will cause damage to the pump tubing.

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CorSolutions Version V2.003
Software. Two software versions have been shipped with your pump. One version is for computers that do
not have LabView installed and the other version is for computers with LabView installed. Please select the
appropriate version for your computer.
Non-LabVIEW Computers:
1. Install the software version for computers without LabVIEW, click on the Setup icon and then
installation will be completed automatically.
or
LAbVIEW Software Computers:
1B. To install the software version for computers currently running the program LabVIEW use the
“CorSolutions Pump App Build” . Copy the entire directory “CorSolutions Pump App Build” with all
its contents, to the computer hard drive. Then click on the application CorSolutionsPump.EXE, and
installation will begin.
Installation of the USB to Serial Driver: For both LabVIEW and non-LabVIEW computers. Please note that the
application requires Administrative Rights so that the communication port (COM) may be configured
correctly for the pump.
Installing the USB to Serial Driver (PC Requires Administrative Rights):
2. Be sure to leave the USB to Serial adapter cable plugged into the USB port with device powered on
while making this change.
3. Run the program “CDM20824_Setup.exe” (You will need administrative rights)
4. Open the PC’s “Device Manager”
5. Expand the "Ports (COM & LPT)" entry (click on the + in the box).
IMPORTANT: If the version for computers not having LabView is accidently installed on a
computer with LabView, corruption of the LabView program is possible.

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CorSolutions Version V2.003
6. Right click on the "USB Serial Port (COMx)" entry, a drop down will appear:
7. Click on the "Port Settings" tab at the top, then click the "Advanced" button.

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CorSolutions Version V2.003
8. The final dialog will appear:
9. The "Latency Timer (msec)" setting is to be changed. Click on it then select and change the value to 2.
10. Click on the OK button, and follow back through the dialogs clicking OK in turn.
2

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CorSolutions Version V2.003
USING YOUR PUMP
Starting the Software. Start the software by clicking on the “CorSolutions” icon shown below.
A box, similar to the one below will then open.
Then select “Scan All Ports”, as indicated below.
A CorSolutions’pump will be detected if present, and will be indicated in the “Device Found” field.

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CorSolutions Version V2.003
Alternativly to the “Scan all ports” method, if the COM port number is known in advance, one may check the
“Check for Device?” box in order to avoid the scanning process.
Once a pump(s) is found, select the “Finished” button at the bottom right of the screen. (If “No Device on
port” occurs thus indicating there is no pump found, check to see the communication cable is connected and
that there is power to the pump.)
The software will then open as indicated below for a single pump (Left) or two pumps (right).

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CorSolutions Version V2.003
Purging the Pump. To purge the pump, place the pump inlet into a suitable vessel containing the aqueous-
based solution, as shown here.
Next remove the fitting to the inlet side of the flow sensor, as shown below. This will relieve the backpressure
in the system during the purge process.
Remove Inlet Line
To Flow Sensor When Purging
At high Flow Rates

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CorSolutions Version V2.003
Then click on the “Mode” button to put the pump in “Purge Mode”. The “Flow Control” light should not be
illuminated and the Purge Mode button will illuminate. In Purge Mode the flow sensor is disabled, and the
pump operates as a traditional peristaltic pump.
Enter a flow rate value of 500 and then click “Set”. This will have the pump run at a fixed rate of 500 µL/min.
Liquid will soon be observed exiting the Flow Sensor inlet fitting, as shown below. Allow for liquid to continue
exiting until all major air bubbles are purged.
Purge Until Liquid Exits
the tube and no air
bubbles are observed

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CorSolutions Version V2.003
Next click the “Stop” button in the software.
Then reconnect the fitting into the flow sensor inlet, ensuring that the tubing is seated past the ferrule’s end
for the PeriNano & PeriMicro pump models. (For the PeriMilli model, insure the tube is flush with the bottom
of the flat bottom ferrule.) The image below shows how the tubing needs to protrude from the fitting end in
order to make a proper connection to the flow sensor.

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CorSolutions Version V2.003
Once the connection to the flow sensor inlet has been made, click the “Set” button to purge the flow sensor.
This should only take 10’s of seconds. Once the flow sensor registers a value approaching the maximum for a
given model, the flow sensor will be filled.
PeriNano = 7 microliters/min
PeriMicro = 50 microliters/min
PeriMilli = 1000 microliters/min
Click the “Stop” button to stop the flow of liquid. And finally, click the “Purge Mode” button to take the pump
out of “Purge Mode”. The flow control light will become illuminated.

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CorSolutions Version V2.003
Setting the Flow Rate. Enter the desired value into the software and then press the “Set Button”.
The pump will accept values ranging from -500 µL/min to 500 µL/min in the Purge Mode. A negative flow rate
means that the flow is being reversed and the pump is aspirating liquid. A positive flow rate means that the
pump is delivering liquid. The software will not register the flow rate entered until the user clicks the “Set”
button. For negative values caution should be used with flow rate to avoid cavitation and degassing. In Flow
Control Mode, the upper and lower flow range will be limited relative to the pump model.
Pump “Stop” versus setting Flow Rate to 0 µL/min. When the “Stop” button is selected in the
software, the power to the motor is turned off. In contrast, setting the flow rate to 0 µL/min keeps the pump
in Flow Control Mode, and the pump will continue to compensate to maintain a flow of 0 µL/min. The ability
to maintain a flow rate of 0 µL/min is useful when multiplexing more than one pump as the pump set to 0
µL/min will compensate for changes in backpressure, eliminating the possibility of back flow.
Timer. The software has a built-in timer that will allow the user to have the pump deliver a flow rate
for a desired period of time. When the time limit has been reached, the software sets the flow rate to 0
µL/min. This feature is useful if a user wants the pump to run for a period of time, but to stop before the user
returns to the lab.
Timer
On/Off
Timer
Set point
Time
Count Down
Actual Flow
Read back
Flow Set Point
Field
Turns Pump
Motor Off
Sets Flow
To On
Flow Control vs.
Purge Mode
Resets
X-Axis
Data Logging
On/Off
Waveform
Entry Mode
Y-Axis
Scaling

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CorSolutions Version V2.003
Fluidic Waveform Generation:
Variable fluid delivery may be achieved by entering the Waveform module of the software. Many
combinations are possible and an example is provided below. Absolute flow rate changes vs. time must be
balanced with the desired period. Avoid using to quick of change where the “Amplitude is too large relative to
the “Period” setting.
Overview of the Waveform module:
Click “Use Waveform”
Select the desired type of
Wave
(Sine may the best type to start
with since it has smooth
transitions)
Amplitude is the min and max
value centered around the
Offset
Offset is the target flow center
value.
Don’t Use the “Duty”
parameter
Example
Period = 30; Offset = 1000; Amplitude = 200
The Sine Wave will be generated over 30 seconds with the flow resulting in 800 to 1200 with the center of the Wave being at 1000
The Wave will be repeated until you hit Stop or reenter into the Waveform dialog Box and un check the “Use Waveform”
Click “OK” to start

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CorSolutions Version V2.003
Using the Waveform Module:
The Waveform Configuration Window will open:
1. Enter the values in the “offset”, “Amplitude”, and “Period” Field
2. Choose the “Type” of Waveform
3. Check the box “Use Waveform”
4. Select the “OK”
Click Here
Waveform
Configuration
Window

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CorSolutions Version V2.003
Trouble Shooting:
Air Bubbles. If air is pulled into the flow sensor, the pump will behave erratically. Always ensure there is
sufficient aqueous solvent in the reservoir and that the inlet tube is submerged in liquid. Furthermore, when
using the pump with a negative flow rate value (in an aspirate mode), ensure that air is not pulled into the
flow sensor. Degassing of the fluid supply is recommended if bubble formation is an issue. Once air bubbles
are introduced in to the system, purging of the pump is recommended.
Small bubbles will be trapped in the Dampener/Bubble Trap tube located below the flow sensor on the
pump front face plate. These apical regions serve as a bubble trap to limit the bubbles entering the flow
sensor. The small trapped bubbles should not affect the performance of the pump.
All major air bubbles should be removed from the system using the purge process. If desired, small trapped
bubbles may be removed from the system by using a plug of air. This can be accomplished by removing the
inlet tube from the liquid source for just a few seconds before returning the inlet tube to the liquid source.
This will generate a plug of air that will sweep out the small trapped bubbles in the system.
Dampener / Air Bubble
Trapping Tube
IMPORTANT:
If the pump is acting erratically where the motor spins back and forth with quick jerky movements,
there is most likely an air bubble in the flow sensor portion of the system. To rid the system of air
bubbles, first try purging the flow sensor, and if the pump still behaves erratically, perform the
entire purge process.

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CorSolutions Version V2.003
MAINTENANCE
The pump should be flushed on occasion with a 5-10% bleach solution for cleaning and to inhibit
bacterial growth. The pump should be stored dry or with the above mentioned solution to avoid bacterial
growth. Avoid the use of incompatible solvents that may react with the Pharmed peristaltic pump tube and
the (Tygon/PVC-based) dampener/bubble trap tube.
The pump rollers should be cleaned when a visible amount of debris is observed on the rollers or when
they do not turn freely.
The Peristaltic pump tubing should be changed annually, but will depend on the amount of use.
Applications:
Valve Loop Injection Displacement Application.
For situations where the pump’s internal volume is too large or where the tubing is not chemically compatible
with the fluids, an optional loop-injection kit is available. See schematic and valve below. With this
configuration, the sample solution may be loaded into a loop that resides between the pump and the
microdevice. Once loaded the pump may be used to displacement the sample from the loop to the
microdevice. This valve loop allows for a small sample volume (based on user defined loop size) to be
introduced to the microdevice with only the transfer tubing to the microdevice contributing to the system
volume. The loop and tubing may be FEP or PEEK providing a relative inert flow path. CorSolutions’ Part
number: ACC-154)
SUPPORT: Please contact customer service by email at [email protected].
Pump
Solution
Waste
Micro-
Device
Pump
Micro-
Device
Sample In Sample In
Solution
Waste
Position A
(Load)
Position B
(Inject)