DSI Individual Cigarette Puff User manual

Individual Cigarette Puff Generator Manual
OVERVIEW
This manual highlights how DSI’s Individual Cigarette
Puff Generator (601-2055-001) can be used to
automate the smoking of cigarettes or electronic
cigarettes to capture direct smoke with the purpose
of exposing conscious animals to the effects of real
cigarette smoke.
DSI Buxco©

2 | P a g e
014010-001 Rev 01
©2019 Data Sciences International.
Table of Contents
Welcome......................................................................................................................................... 3
System Components ....................................................................................................................... 4
Hardware Setup .............................................................................................................................. 4
Physical Hardware..............................................................................................................................................5
Loading Cigarettes..............................................................................................................................................6
Basic holder –NJOY King eCigarette .........................................................................................................................7
JUUL eCigarette Holder .............................................................................................................................................8
Regular Cigarette Holder...........................................................................................................................................9
Operation...................................................................................................................................... 12
Period...............................................................................................................................................................12
Puffs/min..........................................................................................................................................................12
Volume.............................................................................................................................................................12
Recommended Ranges.....................................................................................................................................13
Cigarette Time..................................................................................................................................................14
Smoke Pump ....................................................................................................................................................14
eCigarette mode...............................................................................................................................................14
Service Mode....................................................................................................................................................15
System Operation ......................................................................................................................... 15
Operational Steps for an electronic Cigarette..................................................................................................15
Maintenance................................................................................................................................. 15
Ash Trays ..........................................................................................................................................................16
Cleaning the Cartridges....................................................................................................................................16
Brushing Out the Cartridges and Greasing the o-rings ....................................................................................17
Inspecting all Cartidge O-Rings.........................................................................................................................20
Brushing the Inside of the Transverse Lung Pipe .............................................................................................21

3 | P a g e
014010-001 Rev 01
©2019 Data Sciences International.
Parts List........................................................................................................................................ 24
Replacing the Lung........................................................................................................................ 24
Technical Support ......................................................................................................................... 29
Appendix ....................................................................................................................................... 30
Dimensions.......................................................................................................................................................30
WELCOME

4 | P a g e
014010-001 Rev 01
©2019 Data Sciences International.
Congratulations on joining the community of users worldwide who rely on DSI’s products to perform preclinical
physiologic research and we thank you for your interest in DSI solutions. We are committed to providing you with
quality products and services.
This manual will help you get to know your Individual Cigarette Puff Generator. The structure of the manual was
designed to sequentially guide you through using your DSI system from set up to smoke exposure to routine
maintenance.
WHAT YOU WILL BE LEARNING
1. Understand how the Individual Cigarette Puff Generator is configured to smoke cigarettes or electronic
cigarettes
2. How to connect to exposure systems
3. How to perform crucial routine maintenance to ensure longevity of the system
SYSTEM COMPONENTS
The DSI Buxco© Individual Cigarette Puff Generator is an integrated unit. All mechanical components are housed
in a single chassis and the direct smoke is channeled through internal tubing before exiting an external port. The
major components include:
▪An Inhale Adapter where cigarette holders are inserted (Figure 1)
▪Various holders to connect different types of regular or electronic cigarettes
▪A pump assembly for drawing and delivering direct smoke
▪A display for viewing settings and messages during operation (Figure 1)
▪A menu pad for adjusting operational settings (Figure 1)
▪A power cord
▪Tubing for the delivery of direct smoke
HARDWARE SETUP
To set up the Individual Cigarette Puff Generator, first place the unit on a secure stable bench, stand, cart, or rack
and away from any flammable or heat-sensitive materials. Referring to Figure 1, the menu pad and display can be
seen on the front of the unit to the right. To the left of the menu pad is the inhale adapter where the user will
manually connect the appropriate cigarette holder and insert one cigarette or electronic cigarette. It is
recommended that the Individual Cigarette Puff Generator be located in a fume hood to eliminate indirect smoke
contamination.
The Individual Cigarette Puff Generator can be connected to a variety of apparatuses, including in-line filters, mass
dosing chambers, inhalation towers supplied by DSI, or any number of third-party measurement or exposure

5 | P a g e
014010-001 Rev 01
©2019 Data Sciences International.
systems. Due to the wide assortment of possibilities, this document does not include details on how to connect
the individual Cigarette Puff Generator to any specific apparatus.
PHYSICAL HARDWARE
Figure 1
The left side of the unit can be seen in Figure 2. This side of the unit has the following:
▪Power switch
▪Power connection
▪USB port (for service by DSI Technical Support)
Figure 2

6 | P a g e
014010-001 Rev 01
©2019 Data Sciences International.
The right side of the unit can be seen in Figure 3. This side has the following:
▪The Direct Smoke port
▪Cartridges for the inhalation and exhalation actuators, secured by thumbscrews
Figure 3
Attach the power cord and plug it into a power outlet. Connect tubing to the Direct Smoke port. The tubing is
connected by pushing the end firmly into the compression fitting until it is secure.
Connect a tube from the Direct Smoke port to the exposure chamber.
The Individual Cigarette Puff Generator is now set up and ready to use.
LOADING CIGARETTES
The Individual Cigarette Puff Generator utilizes an adapter on the front of the unit where a variety of holders can
be easily inserted that interface directly with smoking devices, such as Kentucky 3R4F Reference cigarettes (or
equivalent diameter), the NJOY King (or equivalent diameter), or the JUUL electronic cigarette. If your scientific
needs require a different cigarette type or size, please contact your DSI Sales Representative to discuss possible
options.

7 | P a g e
014010-001 Rev 01
©2019 Data Sciences International.
BASIC HOLDER –NJOY KING ECIGARETTE
This holder is always inserted to the front adapter port on the Individual Cigarette Puff Generator. It accepts the
NJOY King eCigarette directly into its central hole, as well as the other holders listed in subsequent sections of this
manual. (Fig 4)
Side View Front View
Figure 4
A duckbill valve should be connected to the back of this holder before connecting it to the Individual Cigarette Puff
Generator (Fig 5). The purpose of this piece is to serve as a one-way passthrough that prevents backflow from the
lung pushing indirect smoke out of the electronic cigarette’s tip. This component should only be used with
electronic cigarettes.
Figure 5
These duckbill valves are considered disposable and each Individual Cigarette Puff Generator comes with 10 valves.

8 | P a g e
014010-001 Rev 01
©2019 Data Sciences International.
WARNING: If smoke is coming out of the tip of an electronic cigarette during the exhalation stage of
puffing, this indicates that the duckbill valve is stuck open. Check the valve, and clean or replace as
necessary.
WARNING: If no smoke is coming out of the Direct Smoke port during the exhalation stage of puffing,
this may indicate that the duckbill valve is stuck closed. Check the valve, and clean or replace as
needed.
Once the duckbill valve is firmly seated on the back of the holder, it can be plugged in to the adapter on the front
of the Individual Cigarette puff Generator and an eCig can be directly plugged into the central orifice. (Fig 6)
Figure 6
JUUL ECIGARETTE HOLDER
This holder is designed to accept the unique shape of the JUUL eCigarette, though any eCig with the same orifice
shape and dimensions will work as well (Fig 7). This holder is simply inserted into the basic holder shown above
(Fig 4).

9 | P a g e
014010-001 Rev 01
©2019 Data Sciences International.
Figure 7
The JUUL eCigarette can then be inserted into the holder. (Fig 8)
Figure 8
REGULAR CIGARETTE HOLDER
This holder is designed to accept the shape of a Kentucky 3R4F Reference Cigarette, though any cigarette with the
same diameter will work as well (Fig 9). This holder is simply inserted into the basic holder shown above (Fig 4).

10 | P a g e
014010-001 Rev 01
©2019 Data Sciences International.
Figure 9
The cigarette can then be inserted into the holder. (Fig 10)
Figure 10