Elisabeth Pharmacon ZEPHYRUS ZVPMS-300 User manual

ZEPHYRUS®Vacuum Pump Manifold System
Instructions for Use
Ref. No.: ZVPMS-300
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Tento dokument je majetkem ELISABETH PHARMACON, spol. s r. o. Je určen ke zveřejnění. Verze: 190620-01
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Content
Content.............................................................................................................................................2
Warnings ..........................................................................................................................................3
Important Notice..............................................................................................................................3
Unpacking.........................................................................................................................................3
Introduction .....................................................................................................................................4
Main Part Diagram ...........................................................................................................................6
Installation and operation................................................................................................................7
Maintenance ..................................................................................................................................13
Troubleshooting.............................................................................................................................14
Ordering information.....................................................................................................................15
Warranty ........................................................................................................................................16
Appendix ........................................................................................................................................17
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3
Warnings
Read this manual before operating the ZEPHYRUS®Vacuum Pump Manifold System and keep the
manual handy for future reference.
Important Notice
This instrument is designed for laboratory use only. Please read this manual carefully before
installing and operating. The instrument shall not be modified in any way. Any modification will
void the warranty and may result in potential safety hazard. We are not responsible for any injury
or damage caused by any non-intended purposes or modification of the instrument without
authorization.
1. Check the voltages specified on the tag plate and ensure it matches the voltage in your
location.
2. Install the instrument in a clean, dust free and ventilated area.
3. Never use the instrument with any corrosive, chemical substances or flammable gas.
4. The pump is not designed to start against applied vacuum. To prevent the damage, make sure
that the pump inlet is exposed to atmospheric pressure before each start. If necessary, the
inlet can be vented out by partially unscrewing the Vacuum Regulator knob on the bottom of
filter cartridge.
5. The pump has a thermal protection feature that automatically shuts-off the pump when it
becomes overheated.
6. The temperature of the surface of pump is very high after use or during work, please do not
touch it to avoid burns.
7. Do not use any lubricants, which may damage the pump.
8. Please discard packing material according to local related regulations.
9. Operating condition of vacuum pump
(a) Ambient temperature: 5~ 40 °C
(b) Relative humidity: 80% RH Max.
(c) Power supply: 100~120V, 50/60Hz or 200~240V, 50/60Hz
(d) Altitude: up to 2000 m
(e) Pollution degree: II
(f) IP30
(g) Overvoltage category: II
(h) Indoor use
Unpacking
Always handle the ZEPHYRUS®Vacuum Pump Manifold System and parts with care.
Check carefully for any shipping damage. In the event of shipping damage, your claim must be
filed with the carrier. Carefully remove the system and all accessories from the carton and remove
any protective pieces. Check the contents of the package to make sure you have received all parts
you have ordered.
Vytvořeno: VIJU Instruction for use ZEPHYRUS® Vacuum Pump Manifold System
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Introduction
The ZEPHYRUS® Vacuum Manifold UNI, is universal vacuum manifold, that was primarily designed
for rapid extraction of DNA/RNA using the 96-well extraction plate format, however you can also
extract NA from up to 24 mini spin columns, 8 midi or maxi columns at the same time using the
Adaptor Board.
The ZEPHYRUS® Vacuum Pump Manifold System, is offered as a set consisting of a ZEPHYRUS®
Vacuum Manifold UNI and a ZEPHYRUS® Vacuum Pump. This system employs the vacuum
created by pump instead of time-consuming centrifugation step that pulls samples through a
membrane. Using 96-well plate format saves your time but also simplifies the entire process and
eliminates repetitive steps, such as dispensing solution, running and stopping centrifugation.
Hint: “This pump/manifold system is very compact, there is no requirement for additional clean
room space to work with extractions, the system easily fits to your existing laminar flow box and
unlike many automatic systems, is takes minimum of bench space.”
The ZEPHYRUS® Vacuum Pump Manifold System can be used with both with 96-well extraction
plate but also with spin columns by inserting columns to Luer connectors on Adapter Board. It is
suitable for those laboratories that require saving time while working with large sample volumes.
⚫Applications
Plasmid DNA, single-stranded phage DNA, RNA, genomic DNA, viral nucleic acids, DNA cleanup
from PCR and other enzymatic reactions.
Hint: “This pump/manifold is compatible with EliGene® Extraction Kits for Manual DNA or
RNA isolation and easily achieves extraction speeds of automatic systems.”
Vytvořeno: VIJU Instruction for use ZEPHYRUS® Vacuum Pump Manifold System
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Model
Standard Package Includes:
Zephyrus Vacuum
Manifold UNI
Zephyrus Vacuum Manifold UNI - plate
Vacuum Manifold
x 1
Spin Column Adaptor Board
x 1
Luer Connector (25/pk)
x 1
Luer Stopper (25/pk)
x 1
Waste Tray
x 1
Allen Wrench
x 1
Sealing Kit (O-ring x 1, Gasket x 1)
x 1
Instruction Manual
x 1
Model
Standard Package Includes:
Zephyrus Vacuum
Pump Manifold
System
Zephyrus Vacuum Pump - Oil Free Vacuum
Pump
x 1
Zephyrus Vacuum Manifold UNI - plate
Vacuum Manifold
x 1
Spin Column Adaptor Board
x 1
Luer Connector (25/pk)
x 1
Luer Stopper (25/pk)
x 1
Waste Tray
x 1
Allen Wrench
x 1
Sealing Kit (O-ring x 1, Gasket x 1)
x 1
Silicone Tube
x 1
Instruction Manual
x 1
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Tento dokument je majetkem ELISABETH PHARMACON, spol. s r. o. Je určen ke zveřejnění. Verze: 190620-01
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Main Part Diagram
Zephyrus Vacuum Manifold UNI (ZVM-300)
Position
Designation
Position
Designation
1
Lower Chamber
5
Vacuum Vent Switch
2
Upper Chamber
6
Vent
3
Gasket
7
Vacuum Connector
4
Top Ring
Zephyrus Vacuum Pump Manifold System (ZVPMS-300)
Position
Designation
Position
Designation
1
Power Switch
4
Vacuum Regulator
2
Muffler
5
Vent
3
Air Inlet
6
Vacuum Connector
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Tento dokument je majetkem ELISABETH PHARMACON, spol. s r. o. Je určen ke zveřejnění. Verze: 190620-01
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7
Installation and Operation
A. Zephyrus Vacuum Manifold UNI Installation
1. Ensure that the surface of gasket and O-ring are free from dirt or debris. Dirty gasket and O-
ring would lead to insufficient hermetic seal.
2. Put a Waste tray in Lower Chamber; assemble the manifold by placing the Upper Chamber on
the top of it.
3. Put 96-well extraction plate or spin column Adaptor Board firmly on the gasket of Upper
Chamber according to the type of extraction kit.
Hint: “When using the Waste Tray inside of the manifold to collect the flow through buffers, the
Waste Tray and the interior parts of Upper and Lower manifold Chambers have to be washed and
cleaned thoroughly after each set of extractions to prevent sample contamination.”
B. Zephyrus Vacuum Pump Manifold System
1. Install the ZEPHYRUS® Vacuum Manifold UNI according to Installation A. 1~3.
2. Turn on the pump and make a test run, block the inlet of the pump and regulate to the desired
vacuum level by adjusting Vacuum Regulator on the bottom of Filter Cartridge (4) then turn
the pump off.
3. Connect the Silicone Tube to a Vacuum Connector of installed ZEPHYRUS® Vacuum Manifold
UNI and inlet of ZEPHYRUS® Vacuum Pump as shown in previous part.
Hint: „To set the appropriate vacuum degree is a key point to achieve efficient extraction. We
suggest setting the system vacuum level according to extraction protocol before starting
extraction.”
96-well Extraction Plate Mini Spin Column x 24 Maxi Spin Column x 8
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C. Zephyrus Vacuum Manifold UNI Installation
Before starting, please make sure the system is installed according to instructions mentioned
above in point A. and B.
When using the extraction or purification kits from other producers, please follow the
“vacuum protocol”in the relevant kit instruction for use manual.
ZEPHYRUS® Vacuum Manifold UNI can be also connected to a local stationary vacuum source
with optional vacuum regulator to adjust the vacuum degree.
Please rinse and clean the entire ZEPHYRUS® Vacuum Manifold UNI instrument according to
the Maintenance instructions after every use to prevent the contamination of your lab.
Hint: “To utilize your plastic waste effectively, you can use the upper or lower part of “used
pipette tip box” as a waste tray before the ecological disposal.”
I. Using the 96-well Extraction Plates
1. Place one unused 96-well extraction plate firmly on the gasket of Upper Chamber.
2. Make sure that the vacuum is switched OFF on the vacuum pump.
3. Load your samples in lysis buffer on the 96-well extraction plate using multichannel pipette.
Hint: “To utilize your 96-well extraction plate effectively (when having few samples), you can
seal off the unused part of the plate with sticky seal foil. The unused part is good for 1 month
from the first use when stored at 5C.”
4. Turn the pump ON to apply the vacuum, press slightly on the edges of extraction plate to
engage the proper vacuum seal. Wait till the entire volume passes through the plate and is
collected in Waste Tray.
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5. When finished, turn the pump OFF. Slowly and carefully open the Vent on the manifold to
release the residual vacuum to prevent cross-contamination.
Hint: “Do not release the manifold vacuum pressure by lifting up the corner of 96-well extraction
plate. This will result in deformation of the manifold gasket and will affect the hermetic seal in
future extractions.”
6. Load the wash buffer on 96-well extraction plate using multichannel pipette.
7. Turn the pump ON to apply the vacuum, press slightly on the edges of extraction plate to
engage the proper vacuum seal. Wait till the entire volume passes through the plate and is
collected in Waste Tray.
8. Repeat the wash steps according to recommendations of kit producers.
9. When finished, turn the pump OFF. Slowly and carefully open the Vent on the manifold to
release the residual vacuum to prevent cross-contamination.
10. Before the elution step remove the 96-well extraction plate and set aside. Disassemble the
manifold and remove the Waste Tray from inside of the manifold (or throw away the pipette lid
with collected flow through as recommended in Hint section).
11. Place the Acrylic spacer block or Adaptor Board on the bottom of the manifold. With the block
or board sitting on the on the bottom, place one 96 Deep Well Collection Plate to collect the
eluate (follow recommendations of kit producers).
12. Assemble the manifold by placing the Upper Chamber on the top of Lower Chamber with 96
Deep Well Collection Plate inside of manifold.
13. Put back the 96-well extraction plate firmly on the gasket of Upper Chamber so the protruding
column tips sit few millimeters above the 96 Deep Well Collection Plate.
14. Apply the Elution buffer and elute your samples into the 96 Deep Well Collection Plate. Use
your samples in succeeding PCR analysis OR perform the elution step with centrifugation to
ensure maximal recovery (follow recommendations of kit producers).
Hint: “You may also remove residual droplets on the bottom of extraction plate by tapping the
top of 96-well extraction plate before releasing the vacuum by Vent.”
15. When finished, turn the pump OFF. Slowly and carefully open the Vent on the manifold to
release the residual vacuum to prevent cross-contamination.
Vytvořeno: VIJU Instruction for use ZEPHYRUS® Vacuum Pump Manifold System
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II. Using the Spin Columns
1. Put spin column Adaptor Board firmly on the gasket of Upper Chamber.
2. Replace the number of Luer Stoppers with Luer Connectors on Adapter Board according to
desired number of used spin columns.
Hint: “ To ensure the proper vacuum level keep the Luer Stoppers in place on Adaptor Board when
working with fewer spin columns.”
3. Insert the spin columns firmly into the Luer connectors. Using same type columns at the same
time will prevent different flow through rate.
4. Make sure that the vacuum is switched OFF on the vacuum pump.
5. Load your samples in lysis buffer onto the columns; turn the pump ON to apply the vacuum,
press slightly on the edges of Adaptor Board to engage the proper vacuum seal. Wait till the
entire volume passes through the plate and is collected in Waste Tray.
4. When finished, turn the pump OFF. Slowly and carefully open the Vent on the manifold to
release the residual vacuum to prevent cross-contamination.
Hint: “Do not release the manifold vacuum pressure by lifting up the corner of the Adaptor Board.
This will result in deformation of the manifold gasket and will affect the hermetic seal in future
extractions.”
6. Load the wash buffer onto the columns; turn the pump ON to apply the vacuum, press slightly
on the edges of Adaptor Board to engage the proper vacuum seal. Wait till the entire volume
passes through the plate and is collected in Waste Tray.
7. Repeat the wash steps according to recommendations of kit producers.
8. When finished, turn the pump OFF. Slowly and carefully open the Vent on the manifold to
release the residual vacuum to prevent cross-contamination.
9. Set the Adaptor Board aside and disassemble the manifold, remove the Waste Tray from
inside of the manifold (or throw away the pipette lid with collected flow through as
recommended in Hint section).
10. Before the elution remove the columns and proceed with centrifugation step to ensure
maximal recovery (follow recommendations of kit producers).
11. Apply the Elution buffer and elute your samples into collection tubes. Use your samples in
succeeding PCR analysis (follow recommendations of kit producers).
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Hint: “You may also remove residual droplets on the bottom of extraction plate by tapping the
top of the Adaptor Board before releasing the vacuum by Vent.”
12. When finished, turn the pump OFF. Slowly and carefully open the Vent on the manifold to
release the residual vacuum to prevent cross-contamination.
1200 ml PES Vacuum Bottle
(optional)
Zephyrus Vacuum Pump Manifold System
assembly diagram
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D. Replace the O-ring and Gasket
When O-ring and gasket are worn, the proper vacuum level cannot be achieved. Please
replace them with new replacement set that is supplied free with this system.
Both O-ring and gasket should be replaced at the same time.
I. O-ring
1. Remove existing O-ring from the bottom of the Upper Chamber.
2. Ensure the new O-ring is free from dirt, debris, and particulate matter.
3. Replace with new O-ring.
II. Gasket
1. Remove the 12 screws located on the bottom of Upper Chamber using supplied Allen wrench.
2. Separate the top ring, remove the old gasket, and clean thoroughly the top ring groove.
3. Put the new gasket into the groove and put the top ring back. Please ensure that the bottom
of the top ring groove is aligned with cover gasket.
4. Put back the 12 screws and tighten them at corners slightly and lock other screws back. Do not
lock too tight too strong otherwise distorted top ring may cause a poor vacuum.
Vytvořeno: VIJU Instruction for use ZEPHYRUS® Vacuum Pump Manifold System
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Tento dokument je majetkem ELISABETH PHARMACON, spol. s r. o. Je určen ke zveřejnění. Verze: 190620-01
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Maintenance
1. Please clean the ZEPHYRUS® Vacuum Manifold UNI with water and either air dry or wipe with
paper towels after every use. Do not use any harsh chemicals including solvent, bleach, or
abrasives.
2. In case you need to remove contaminating nucleic acids we are recommending to use the
EliGene® Lab Cleaner B whish is a granular agent with maximum efficiency for degrading nucleic
acids (DNA, RNA) and DNAse / RNAse. The prepared solution can be used for cleaning plastic
surfaces of instruments, Plexiglas, table tops, glass surfaces, pipettes (the solution does not
damage plastics).
3. ZEPHYRUS® Vacuum Manifold UNI instrument is autoclavable including silicone tube and all
accessories, please clean them thoroughly and autoclave (121°C, 1 bar, 20 min) if needed.
4. The Vacuum Pump is not autoclavable!!! Please clean the surface with pure water, 75% ethanol
or Isopropanol (IPA).
5. After finishing the experiment, please keep pumping the air for at least 2 minutes to withdraw
the residual steam that may be trapped inside of Vacuum Pump ducts.
6. When the gasket, O-ring are worn off or damaged, please replace them immediately. Do not
apply any silicone oil or vacuum grease to the gaskets or other parts of the ZEPHYRUS® Vacuum
Manifold UNI!!!
Vytvořeno: VIJU Instruction for use ZEPHYRUS® Vacuum Pump Manifold System
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Tento dokument je majetkem ELISABETH PHARMACON, spol. s r. o. Je určen ke zveřejnění. Verze: 190620-01
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Troubleshooting
Problem
Reason and Solution
Pump fails to start
Wrong power supply →Reconnect to specified power.
Vacuum exists →Release vacuum and restart pump.
Switch is broken →Contact distributor for assistance.
Poor vacuum
Improper vacuum setting →Reset vacuum by regulator.
Clogged muffler →Clean or replace a new muffler.
Unused Luer connectors →Replace with Luer stoppers.
Opened vent valve →Close the vent valve.
Cracked plates or adaptor board →change for a new one.
Loose stoppers →Re-plug the connectors and stoppers.
Dirty or damage O-ring and gasket →Clean or replace it.
Vytvořeno: VIJU Instruction for use ZEPHYRUS® Vacuum Pump Manifold System
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Tento dokument je majetkem ELISABETH PHARMACON, spol. s r. o. Je určen ke zveřejnění. Verze: 190620-01
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15
Ordering information
ZVPMS-300 ZEPHYRUS® Vacuum Pump Manifold System
ZVP-300 ZEPHYRUS® Vacuum Pump
ZVM-300 ZEPHYRUS® Vacuum Manifold UNI
ZVM-300 –1 Spin Column Adaptor Board (24 hole)
ZVM-300 –2 Spin Column Adapter Board - Acrylic (24 holes)
ZVM-300 –3 Luer Connector (25/pk)
ZVM-300 –4 Luer Stopper (25/pk)
ZVM-300 –5 Silicone Tube, O 6 x 12 mm, 30 cm
ZVM-300 –6 Silicone Tube, O 6 x 12 mm, 100 cm
ZVM-300 –7 Waste Tray
ZVM-300 –8 Sealing kit (includes O-ring, gasket )
ZVM-300 –9 Allen wrench
ZVM-300 –10 Vacuum regulator
ZVM-300 –11 1200 ml PES Vacuum Bottle
ZVM-300 –12 Muffler for Zephyrus Vacuum Pump ZVP-300
ZVM-300 –13 96 Deep Well Collection Plate; 2,2 ml; h44 mm
ZVM-300 –14 96 Deep Well Collection Plate; 2,2 ml; h41 mm
ZVM-300 –15 96 Well Collection Plate; 1,2 ml; h24mm
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Tento dokument je majetkem ELISABETH PHARMACON, spol. s r. o. Je určen ke zveřejnění. Verze: 190620-01
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16
Warranty
The ZEPHYRUS® Vacuum Pump Manifold System is warranted against defects in materials,
workmanship, and normal use for 1 year. If any defects occur in the instrument or accessories
during this warranty period, Elisabeth Pharmacon Ltd. will repair/replace the defective
parts/replace the instrument at its discretion without charge. Warranty is excluded in the case of
following:
-unauthorized repair
-use of spare parts by anyone other than Elisabeth Pharmacon Ltd.
-used in the manner not described in this manual
-damage caused by accident, neglect, misuse, improper service, natural forces, or other
causes not arising from defects in original material or workmanship
Vytvořeno: VIJU Instruction for use ZEPHYRUS® Vacuum Pump Manifold System
Místo uložení: https://www.elisabeth.cz/produkt-zephyrus-vacuum-pump-manifold-system-cz.html
Tento dokument je majetkem ELISABETH PHARMACON, spol. s r. o. Je určen ke zveřejnění. Verze: 190620-01
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17
Appendix
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Tento dokument je majetkem ELISABETH PHARMACON, spol. s r. o. Je určen ke zveřejnění. Verze: 190620-01
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