Jasco PU-1580 User manual

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P/N: 0302-0375B
MODEL PU-1580
Intelligent HPLC Pump
Operation Manual

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i
Safety Considerations
To ensure operation safety, this instrument must be operated correctly and
maintained regularly according to schedule. Carefully read to fully understand all
safety precautions in this manual before operating the instrument. This manual
denotes precautions against actions that can result in hazardous situations or
equipment damage by using the signal words WARNING, CAUTION, and Note.
(1) Safety symbols
Instruction manual symbol. If the product is marked with this symbol,
refer to the instrument manuals to protect the instrument against
damage.
WARNING A WARNING indicates an potentially hazardous situation which, if not
avoided, could result in death or serious injury
.
CAUTION A CAUTION indicates a potentially hazardous situation which, if not
avoided, may result in minor or moderate injury. It may also be used
to alert against unsafe practices.
Do not proceed beyond a WARNING or CAUTION notice until you
understand the hazardous conditions and have taken the appropriate
steps.
Note A Note provides additional information to aid the operator in obtaining
optimal instrument performance.
Pressurized, hazardous solvents are used in high-performance liquid
chromatography. Always follow the proper laboratory procedures to ensure operator
safety. Always wear goggles, gloves and protective clothing when operating the
instrument, especially when injecting sample and opening valves.

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ii
(2) Warning Labels
Warning labels are attached at several locations on this instrument. Do not remove,
deface or damage the warning labels. If a warning label peels off the instrument or
becomes illegible, contact your local JASCO distributor (see list on the back cover of
this manual) for a replacement label. Be sure to indicate the part number (P/N) on
the label.
(1) Fuse Warning Label (P/N: 0822-0120A)
Fuse ratings are contained in the maintenance manual.
(2) Electrical Shock Warning Label (P/N: 0822-0125A)
Electrical shock may occur if the instrument is not
properly grounded.
Figure 1 Rear Panel
ONLY WITH FUSE OF THE SPECIFIED
AGAINST RISK OF FIRE, REPLACE
FOR CONTINUED PROTECTION
TYPE AND CURRENT RATINGS.
WARNING
WARNING
MAINS PLUG GROUND PIN
OR GROUND TERMINAL
MUST BE CONNECTED
TO GROUND.
FUSE
PUSH
FUSE
PUSH
T1.6A
T2.5A
100‑120V
220‑240V
FUSE
1
2
3
4
5
6
7
PRESS.ZERO
ADJ.
EVENT
VALVE
GRAD.
LOWPRESS.
8
ACINPUT
GND
FUSE
RS‑232C
R
R
(2) Electrical shock
warning label
(1) Fuse warning label
ONLY WITH FUSE OF THE SPECIFIED
AGAINST RISK OF FIRE, REPLACE
FOR CONTINUED PROTECTION
TYPE AND CURRENT RATINGS.
WARNING

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iii
Limited Warranty
Products sold by JASCO, unless otherwise specified, are warranted for a period of
one year from the date of shipment to be free of defects in materials and
workmanship. If any defects in the product are found during this warranty period,
JASCO will repair or replace the defective part(s) or product free of charge.
THIS WARRANTY DOES NOT APPLY TO DEFECTS RESULTING FROM THE
FOLLOWING:
1) IMPROPER OR INADEQUATE INSTALLATION
2) IMPROPER OR INADEQUATE OPERATION, MAINTENANCE, ADJUSTMENT
OR CALIBRATION
3) UNAUTHORIZED MODIFICATION OR MISUSE
4) USE OF CONSUMABLE PARTS NOT SUPPLIED BY AN AUTHORIZED
JASCO DISTRIBUTOR
5) CORROSION DUE TO THE USE OF IMPROPER SOLVENTS, SAMPLES, OR
DUE TO SURROUNDING GASES
6) ACCIDENTS BEYOND JASCO’S CONTROL, INCLUDING NATURAL
DISASTERS
This warranty does not cover the consumable parts listed below:
1) Deuterium lamp, tungsten lamp, xenon lamp and other light sources
2) Mirrors in the light source section, and cell windows
3) Fuses, batteries, glassware, chart paper and ink
4) Plunger seals, needle seals, cell window gaskets, valve seals, disk seals and
other seal materials
5) Tubing and fittings (e.g., ferrules, compression screws), and filters (e.g., inlet
filters, line filters, other solvent filters)
6) Pre-columns and guard columns
THE WARRANTY FOR ALL PARTS SUPPLIED AND REPAIRS PROVIDED
UNDER THIS WARRANTY EXPIRES ON THE WARRANTY EXPIRATION DATE
OF THE ORIGINAL PRODUCT. FOR INQUIRIES CONCERNING REPAIR
SERVICE, CONTACT YOUR JASCO DISTRIBUTOR AFTER CONFIRMING THE
MODEL NAME AND SERIAL NUMBER OF YOUR INSTRUMENT.

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iv
Regulatory Statements
CE Notice:
The symbol indicates that this JASCO system complies with the EMC
(Electromagnetic Compatibility) and Low Voltage Directives of the European
Community. Furthermore, this symbol indicates that this JASCO system meets the
relevant basic safety and health requirements of the EC Directive based on the
following technical standards:
•EN55011---- “Limits and Methods of Measurement of Radio Interference
Characteristics of Information Technology Equipment.” ----
Group 1, Class A.
WARNING: This is a Class A product. In a domestic environment this product may
cause radio interference, in which case the user may be required to
take adequate measures.
•EN50082-1---- “Electromagnetic compatibility”----Generic immunity standard
Part 1: Residential, commercial, and light industry.
•IEC1000-4-2---- “Electromagnetic compatibility for industrial-process
measurement and control equipment Part 2: Electrostatic
discharge requirements.”----Severity level 3.
•IEC1000-4-3---- “Electromagnetic compatibility for industrial-process
measurement and control equipment Part 3: Radiated
electromagnetic field requirements.”----Severity level 2.
•IEC1000-4-4---- “Electromagnetic compatibility for industrial-process
measurement and control equipment Part 4: Electrical fast
transient/burst requirements.”----Severity level 2.
•IEC1010-1---- 1990 + Amd.1:1992 + Amd.2: 1995----Safety requirements for
electrical equipment for measurement, control and laboratory
use.
•A “Declaration of Conformity” in accordance with the above standards has been
made and is on file at JASCO EUROPE srl, Via Confalonieri 25, 22060
CREMELLA (Lc), Italy.

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v
Preface
This operations manual serves as your guide book for using this instrument. It is
intended to instruct first-time users on how to properly use the instrument, and to
serve as a reference for experienced users.
Before using the instrument, please read this operations manual carefully, and make
sure that the contents are fully understood. This manual should be easily accessible to
the operator at all times during instrument operation. When not using the instrument,
keep this manual stored in a safe place. Should this operations manual be lost, order a
replacement from your local JASCO distributor.
Installation Conditions
To ensure operation safety, observe the following conditions:
(1) Do not operate the instrument under voltage fluctuations exceeding 10% of
the recommended line voltage. Otherwise, the instrument may not function
properly.
(2) Use a three-pronged electrical outlet with a ground.
(3) Operate the instrument under a temperature range of 10°∼35°C.
(4) Operate the instrument under a humidity range of 35% ∼85% (RH). If ambient
humidity exceeds 85% (RH), water vapor may deteriorate optical components.
(5) Operate the instrument under an atmospheric pressure of 750 ∼1060 hPa.
(6) Avoid strong magnetic fields and sources of high frequency. The instrument
may not function properly when near a strong magnetic field or high frequency
source.
(7) Avoid vibration from vacuum pumps, electric motors, processing equipment
and machine tools.
(8) Avoid dust and corrosive gas. Do not install the instrument in a location where
it may be exposed to dust, especially in locations exposed to outside air or
ventilation outlets that discharge dust particles.
(9) Do not install the instrument in a location where it may be exposed to direct
sunlight.
(10) Ensure that no air conditioner blows air directly onto the instrument. This may
inhibit stable measurement.
Note: The above conditions do not ensure optimal performance of this instrument.

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vi
Maintenance
Contact your local JASCO distributor for instrument maintenance. In addition,
contact a JASCO distributor before moving the instrument to another location.
Consumable parts should be ordered according to part number from your local
JASCO distributor. If a part number is unknown, give your JASCO distributor the
model name and serial number of your instrument.
Do not return contaminated products or parts that may constitute a health
hazard to JASCO employees.
Notices
(1) JASCO SHALL NOT BE HELD LIABLE, EITHER DIRECTLY OR INDIRECTLY,
FOR ANY CONSEQUENTIAL DAMAGE INCURRED AS A RESULT OF
PRODUCT USE.
(2) PROHIBITIONS ON THE USE OF JASCO SOFTWARE:
z COPYING SOFTWARE FOR PURPOSES OTHER THAN BACKUP
z TRANSFER OR LICENSING TO A THIRD PARTY THE RIGHT TO USE
SOFTWARE
z DISCLOSURE OF CONFIDENTIAL INFORMATION REGARDING
SOFTWARE
z MODIFICATION OF SOFTWARE
z USE OF SOFTWARE ON MULTIPLE WORKSTATIONS, NETWORK
TERMINALS, OR BY OTHER METHODS (NOT APPLICABLE UNDER A
NETWORK LICENSING AGREEMENT CONCLUDED WITH JASCO)
(3) THE CONTENTS OF THIS MANUAL ARE SUBJECT TO CHANGE WITHOUT
NOTICE.
(4) THIS INSTRUCTION MANUAL IS CONSIDERED TO BE COMPLETE AND
ACCURATE AT PUBLICATION.

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vii
TABLE OF CONTENTS
Safety Considerations.............................................................................. i
Limited Warranty.....................................................................................iii
Regulatory Statements ...........................................................................iv
Preface ....................................................................................................v
Maintenance ...........................................................................................vi
TABLE OF CONTENTS.........................................................................vii
1. Product Description and Specifications............................................ 1
1.1. Product Description ......................................................................................... 1
1.2. Specifications .................................................................................................. 1
2. Part Names and Descriptions .......................................................... 4
2.1. Front Panel ...................................................................................................... 4
2.2. Operation Panel............................................................................................... 5
2.3. Rear Panel....................................................................................................... 6
2.4. Right Side Panel..............................................................................................8
2.5. Bottom Panel ................................................................................................... 9
3. Power-ON and Power-OFF............................................................ 10
3.1. Power-ON and Diagnostic Test ..................................................................... 10
3.2. Power-OFF .................................................................................................... 11
4. Operations in Normal Operation Mode........................................... 12
4.1. Switching Between Constant Flow Mode and Constant Pressure Mode ....... 12
4.2. Constant Flow Mode...................................................................................... 12
4.2.1. Changing the parameters ......................................................................... 12
4.2.2. Constant flow rate start and stop..............................................................14
4.2.3. Error messages during pumping .............................................................. 15
4.3. Constant Pressure Mode ............................................................................... 15
4.3.1. Changing the parameters ......................................................................... 15
4.3.2. Constant pressure start and stop ............................................................. 16
5. Operations in Program Mode......................................................... 17
5.1. Description..................................................................................................... 17
5.2. Setting the File Number (Loading Files) ........................................................ 18
5.3. Switching Between Normal Operation Mode and Program Mode.................. 18
5.4. Gradient mode confirmation .......................................................................... 19
5.5. Editing a Program in High-pressure Gradient Mode ......................................20
5.5.1. Editing the initial conditions ...................................................................... 20
5.5.2. Editing time programs .............................................................................. 21
5.5.3. Example of a high-pressure gradient program ......................................... 25
5.6. Low-pressure gradient mode ......................................................................... 26
5.6.1. Editing the initial conditions ...................................................................... 26

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viii
5.6.2. Editing time programs .............................................................................. 28
5.6.3. Example of a low-pressure gradient program........................................... 31
5.7. High- or low-pressure gradient operations..................................................... 32
5.7.1. High- or low-pressure gradient program execution................................... 32
5.7.2. Parameter changes while the gradient program is running ......................33
5.7.3. Operation in conjunction with an Autosampler ......................................... 34
6. Special Operations .......................................................... .................36
6.1. Timer functions ......................................................................................... .... 36
6.1.1. OFF timer setting ([SHIFT][1]) ............................................................. .... 36
6.1.2. ON timer setting ([SHIFT][2])............................................................... .... 37
6.2. Flow rate correction ([SHIFT][3])............................................................... ....38
6.3. Setting pump numbers ([SHIFT][4]).......................................................... .... 38
6.4. Switching between high- and low-pressure gradient modes ([SHIFT][6]) .... 39
6.5. Pressure sensor signal ([SHIFT][6]).......................................................... .... 40
6.6. Adjustment of LPG COMP [SHIFT][7]....................................................... ....41
6.6.1. Setting LPG COMP ............................................................................. .... 41
6.6.2. Procedure for recalibrating the LPG COMP value.............................. ..... 41
6.7. Using the Optional Semi-Micro Kit ([SHIFT][.]) ......................................... .... 42
6.8. Registering Serial Numbers...................................................................... .... 42

1
1. Product Description and Specifications
1.1. Product Description
The Model PU-1580 Intelligent Pump is a high performance, high functionality pump for
HPLC. The pump provides extremely consistent solvent delivery via SSQD (Slow Suction
Quick Delivery).
The pump may be operated in normal operation mode or program mode. Normal
operation mode offers a constant flow rate or pressure. In program mode, selection of
composition, flow rate, solvent selection valves, and event relays can be performed
according to a user-defined time program.
For gradient pumping, both high-pressure (mixing) and low-pressure (mixing) methods are
available. The appropriate method depends on the system being used.
(1) High-pressure (mixing) gradient (HPG)
•For systems having two pumps and one mixer, 2-solvent high-pressure gradients
can be created.
•For systems having three pumps, one mixer, and HPLC instrument control software
(HSS), 3-solvent high-pressure gradients can be created.
(2) Low-pressure (mixing) gradient (LPG)
•For systems having one pump and one low-pressure gradient unit, 3-solvent low-
pressure gradients can be created.
•For systems having HPLC instrument control software (HSS), gradient pumping is
performed using the HSS. For systems that do not include HSS, gradient pumping
is performed using the time program functions of the pumps.
Other functions include
•Automatic pump shut-off
•Maintenance functions: self-diagnostic functions, flow rate and back pressure
correction, plunger washing, etc.
1.2. Specifications
Pumping method: SSQD (Slow Suction Quick Delivery) method
Pumping mode: Fixed flow rate/fixed pressure
(can be selected using a control key)
For constant flow rate analysis:
Flow rate setting range: 0.001 ~ 10 ml/min
Flow rate setting step: 0.001 ml/min