Kendro Sorvall Cellwasher 2 User manual

PN04505-32
SORVALL
Cellwasher 2
®
OPERATING
INSTRUCTIONS

OPERATING
INSTRUCTIONS
SORVALL Cellwasher 2
Cell Washing
System
Kendro Laboratory Products
Asheville, North Carolina
U.S.A.
®
PN04505-32
Issued January 2005

Table of Contents SORVALL®Centrifuges
This manual is a guide to the use of the
SORVALL®Cellwasher 2 Cell Washing System
Data herein has been verified and is believed adequate for the intended use of the Cellwasher 2.
Because failure to follow the recommendations set forth in this manual could produce personal
injury or property damage, always follow the recommendations set forth herein. Kendro does not
guarantee results and assumes no obligation for the performance of centrifuges or other products
that are not used in accordance with the instructions provided. This publication is not a license to
operate under, nor a recommendation to infringe upon, any process patents.
The SORVALL Cellwasher 2 is intended to be used in strict accordance with The American
Association of Blood Banks' guidelines defined in the most recent edition of the AABB Technical
Manual (published by AABB Press. Bethesda, MD).
Publications prior to the Issue Date of this manual may contain data in apparent conflict with that
provided herein. Please consider all data in this manual to be the most current.
WARNING , CAUTION , and NOTE within the text of this manual are used to emphasize
important and critical instructions.
WARNING informs the operator of a hazard or an unsafe practice that could result in
personal injury, affect the operator’s health, or contaminate the environment.
CAUTION informs the operator of an unsafe practice that could result in damage of
equipment.
NOTE highlights essential information.
CAUTION and WARNING are accompanied by a hazard symbol
!
and appear in the left
sidebar near the information they correspond to.
©1988,1995,1996,1998,1999,2002,2003,2004,2005 by Kendro Laboratory Products
ii

Cellwasher 2 Table of Contents
Important Safety Information
Certain potentially dangerous conditions are inherent to the use of all centrifuges. To ensure
safe operation of this centrifuge, anyone using it should be aware of all safe practices and take
all precautions described below and throughout this manual.
WARNING
When using radioactive, toxic, or pathogenic materials, be aware of all characteristics of the
materials and the hazards associated with them in the event leakage occurs during cell washing. If
leakage does occur, neither the Cellwasher 2 nor the rotor can protect you from particles dispersed
in the air. To protect yourself, we recommend additional precautions be taken to prevent exposure
to these materials, e.g., use of controlled ventilation or isolation areas.
Always be aware of the possibility of contamination when using radioactive, toxic, or pathogenic
materials. Take all necessary precautions and use appropriate decontamination procedures if
exposure occurs.
Never use any material capable of producing flammable or explosive vapors, or creating extreme
exothermic reactions.
Use only tubes that meet the criteria provided in this manual (see Tube Selection and Use, Chapter
3). Results may be affected if tubes are not within specifications. If tubes are of inadequate strength
so that tube breakage occurs, sample and test results will be lost, the rotor and distributor could be
damaged, and during required cleanup, contact with sharp glass fragments could result in personal
injury.
CAUTION
Do not operate Cellwasher 2 without a rotor properly installed on the drive shaft. See Rotor and
Distributor Installation, Chapter 2.
Do not operate the Cellwasher 2 with a rotor out of balance. To do so can cause damage to the
Cellwasher 2 drive assembly. See Rotor Loading and Balancing, Chapter 2.
We recommend using Coomb's control cells to confirm all negative results.
The Cellwasher 2 can be damaged if connected to the wrong voltage. Check the voltage before
plugging the instrument into a power source. Kendro is not responsible for incorrect installation.
Always maintain the Cellwasher 2 in the recommended manner. See Chapter 4, Maintenance.
!
Important Safety Information
!
iii

Table of Contents SORVALL®Centrifuges
Page
Safety Information Page . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . iii
Chapter 1. DESCRIPTION
Cellwasher 2 Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1
Applications Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1
Cellwasher 2 Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2
Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4
Chapter 2. INSTALLATION
Unpacking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1
Location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1
Environmental Conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2
Electrical Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2
Tubing Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2
Rotor and Distributor Installation . . . . . . . . . . . . . . . . . . . . . . 2-5
Rotor Loading and Balancing . . . . . . . . . . . . . . . . . . . . . . . . . 2-7
Chapter 3. OPERATION
Controls and Indicators . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-1
Principles of Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3
Tube Selection and Use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-5
Priming the System & Adjusting Saline Fill Volume . . . . . . . 3-6
Auto Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-8
High and Low Speed Operation . . . . . . . . . . . . . . . . . . . . . . . 3-10
Tube Breakage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-11
Momentary Power Interruption . . . . . . . . . . . . . . . . . . . . . . . . 3-12
Emergency Sample Recovery . . . . . . . . . . . . . . . . . . . . . . . . . 3-12
Chapter 4. MAINTENANCE
Preventive Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-1
Sevice Decontamination Policy . . . . . . . . . . . . . . . . . . . . . . . . 4-5
Parts Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-7
Tubing Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-7
Fuse Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-7
Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-7
iv
Table of Contents

Cellwasher 2 Table of Contents
APPENDIX
Warranty
Index
Decontamination Certificates
List of Illustrations
Figure Page
2-1. Cellwasher 2 Tubing Diagram . . . . . . . . . . . . . . . . . . 2-3
2-2. Stainless Steel Rotating Bowl Installation . . . . . . . . . 2-6
2-3. Distributor Installation . . . . . . . . . . . . . . . . . . . . . . . . 2-6
2-4. Tube Placement for Balancing Less Than 12 Tubes . 2-7
3-1. Cellwasher 2 Controls and Indicators . . . . . . . . . . . . 3-3
3-2. Location of Mechanical Override . . . . . . . . . . . . . . . 3-12
4-1. Collecting Ring Assembly, Exploded View . . . . . . . . 4-3
4-2. Location of Decant Coil and Drive Ring . . . . . . . . . . 4-10
4-3. Location of Lift Plate Ring . . . . . . . . . . . . . . . . . . . . 4-10
List of Tables
Table Page
1-1. Accessories Supplied . . . . . . . . . . . . . . . . . . . . . . . . . 1-4
2-1. Parts Location: Tubing Diagram . . . . . . . . . . . . . . . . 2-3
3-1. Cellwasher 2 Controls and Indicators . . . . . . . . . . . . 3-1
4-1. Troubleshooting Chart: Problem A . . . . . . . . . . . . . . 4-8
4-2. Troubleshooting Chart: Problem B . . . . . . . . . . . . . . 4-8
4-3. Troubleshooting Chart: Problem C . . . . . . . . . . . . . . 4-9
4-4. Troubleshooting Chart: Problem D . . . . . . . . . . . . . . 4-9
4-5. Applications Troubleshooting Chart:
False Negative Results . . . . . . . . . . . . . . . . . . . . . . . 4-11
4-6. Applications Troubleshooting Chart:
False Positive Results . . . . . . . . . . . . . . . . . . . . . . . . 4-12
4-7. Applications Troubleshooting Chart:
Inconsistent Reaction Strength. . . . . . . . . . . . . . . . . 4-13
v/vi

Cellwasher 2 Description
1-1
Chapter 1: DESCRIPTION
This manual contains information required to operate and maintain the SORVALL®Cellwasher 2 Cell
Washing System. If you require additional information regarding operation or maintenance, please contact
Kendro for assistance. In the United States, call Kendro toll-free 1-800-522-7746; outside the United
States, contact the nearest Kendro office (see back cover) or your local representative for SORVALL®
products. SORVALL®product information is available on our internet web site at http://www.kendro.com
or http://www.kendro.de.
* The Cellwasher 2 is not designed to automatically separate donor whole blood to create
the necessary 2 to 5% red blood cell/saline suspension. The Cellwasher 2 may, however,
be used as a centrifuge to manually separate red blood cells from whole blood.
Cellwasher 2 Description
The Cellwasher 2 is designed to perform the washing phase of the
Coomb's Procedure automatically. In normal AUTO mode opera-
tion, after placing two or three drops of a pre-prepared 2 to 5% red
blood cell/saline suspension* into each tube, the Cellwasher 2 will
automatically add saline, spin at high speed to concentrate the cells,
decant the saline, and agitate to resuspend the washed cells for the
addition of Coomb's reagent. The Cellwasher 2 is also capable of
being used manually as a low speed centrifuge, spinning at either of
two preset speed ranges (LOW speed or HIGH speed mode, see
Cellwasher 2 Specifications on next page).
The Cellwasher 2 combines a peristaltic saline pump and a wet
chamber centrifuge in one cabinet. The centrifuge portion uses a
dedicated DA-12 Rotor, which is a unique dual-angle swinging-
bucket rotor that is designed to accommodate up to 12 each of
either 3 ml or 5 ml tubes. The Cellwasher 2 is microprocessor-
controlled, featuring a front control panel with sensor-touch keys
and a digital timer for the wash cycle. Other features include a
saline-detect system with an audible low-saline warning, and an
agitate cycle that ensures complete resuspension of cells and
eliminates manual agitation.
Applications Information
The Cellwasher 2 is intended to be used in routine blood work to
speed up and simplify the procedures for determining human blood
groups and types. Often, the results of blood tests made using the
Cellwasher 2 determine donor/patient compatibility for blood trans-
fusions, and the results of these tests must be accurate to prevent
serious transfusion reactions. If testing procedures provided by the
reagent manufacturers are not followed exactly, or if test materials
are outdated, accurate test results cannot be guaranteed.

Description SORVALL®Centrifuges
1-2
False Coomb's readings can be caused by any of the following:
• Improper ratio of patient serum and cells.
• Incomplete washing of cells.
• Contaminated saline or supply tubing.
• Improper decanting of saline solutions.
• Inactive Coomb's serum.
• Failure to add Coomb's serum to a tube.
• Improper final centrifugation.
• Inaccurate reading or recording of results.
While the Cellwasher 2 provides accurate washing, decanting, and a
proper speed for the final spin, it does not eliminate all potential
errors. Proper laboratory procedures require adequate controls of all
tests performed with this instrument (read the WARNINGS). Refer to
Tables 4-5, 4-6, and 4-7 for applications troubleshooting informa-
tion. When using the Cellwasher 2, always follow standard labora-
tory procedures in handling and disposing of blood serum, and
observe safety precautions and good laboratory practices.
Cellwasher 2 Specifications
Dimensions:
Depth . . . . . . . . . . . . . . . . . . . . . . . . . . . .35.6 cm (14.0 inches)
Width . . . . . . . . . . . . . . . . . . . . . . . . . . . .31.8 cm (12.5 inches)
Height: Lid closed . . . . . . . . . . . . . . . . . .36.8 cm (14.5 inches)
Lid open . . . . . . . . . . . . . . . . . . .57.2 cm (22.5 inches)
Mass (Weight): . . . . . . . . . . . . . . . . . . . .16.4 kg (36.0 lbs)
Motor Type: . . . . . . . . . . . . . . . . . . . . . . .3-speed induction
Speeds:*
60 Hz Models: Low 1100 to 1200 rpm
High 3500 to 3600 rpm
Decant 550 to 600 rpm
50 Hz Models: Low 1400 to 1500 rpm
High 2900 to 3000 rpm
Decant 550 to 600 rpm
Length of Modes:
Automatic . . . . . . . . . . . . . . . . . . . . . . . . .Each multi-step wash cycle
is 80 seconds, except on
100V 50 Hz models, which
have 90-second cycles.
Manual . . . . . . . . . . . . . . . . . . . . . . . . . . .High or low, optional, up to
999 seconds if timed; high
speed indefinite if in HOLD
!
W A R N I N G
Werecommendusing
Coomb's control cells to confirm all
negativeresults.
* Speed in revolutions per minute (rpm) is related to angular velocity, ω , according to the
following:
ω = (rpm) = (rpm) (0.10472)
Where w= rad/s. All further references in this manual to speed will be designated as rpm.
2π
60
()
!
W A R N I N G
TheSORVALLCellwasher2
is intended to be used in strict accor-
dance with The American Association
of Blood Banks' guidelines defined in
the most recent edition of the AABB
TechnicalManual(publishedbyAABB
Press. Bethesda, MD).