Kendro Sorvall Cellwasher 2 User manual

PN04505-32
SORVALL
Cellwasher 2
®
OPERATING
INSTRUCTIONS

OPERATING
INSTRUCTIONS
SORVALL Cellwasher 2
Cell Washing
System
Kendro Laboratory Products
Asheville, North Carolina
U.S.A.
®
PN04505-32
Issued January 2005

Table of Contents SORVALL®Centrifuges
This manual is a guide to the use of the
SORVALL®Cellwasher 2 Cell Washing System
Data herein has been verified and is believed adequate for the intended use of the Cellwasher 2.
Because failure to follow the recommendations set forth in this manual could produce personal
injury or property damage, always follow the recommendations set forth herein. Kendro does not
guarantee results and assumes no obligation for the performance of centrifuges or other products
that are not used in accordance with the instructions provided. This publication is not a license to
operate under, nor a recommendation to infringe upon, any process patents.
The SORVALL Cellwasher 2 is intended to be used in strict accordance with The American
Association of Blood Banks' guidelines defined in the most recent edition of the AABB Technical
Manual (published by AABB Press. Bethesda, MD).
Publications prior to the Issue Date of this manual may contain data in apparent conflict with that
provided herein. Please consider all data in this manual to be the most current.
WARNING , CAUTION , and NOTE within the text of this manual are used to emphasize
important and critical instructions.
WARNING informs the operator of a hazard or an unsafe practice that could result in
personal injury, affect the operator’s health, or contaminate the environment.
CAUTION informs the operator of an unsafe practice that could result in damage of
equipment.
NOTE highlights essential information.
CAUTION and WARNING are accompanied by a hazard symbol
!
and appear in the left
sidebar near the information they correspond to.
©1988,1995,1996,1998,1999,2002,2003,2004,2005 by Kendro Laboratory Products
ii

Cellwasher 2 Table of Contents
Important Safety Information
Certain potentially dangerous conditions are inherent to the use of all centrifuges. To ensure
safe operation of this centrifuge, anyone using it should be aware of all safe practices and take
all precautions described below and throughout this manual.
WARNING
When using radioactive, toxic, or pathogenic materials, be aware of all characteristics of the
materials and the hazards associated with them in the event leakage occurs during cell washing. If
leakage does occur, neither the Cellwasher 2 nor the rotor can protect you from particles dispersed
in the air. To protect yourself, we recommend additional precautions be taken to prevent exposure
to these materials, e.g., use of controlled ventilation or isolation areas.
Always be aware of the possibility of contamination when using radioactive, toxic, or pathogenic
materials. Take all necessary precautions and use appropriate decontamination procedures if
exposure occurs.
Never use any material capable of producing flammable or explosive vapors, or creating extreme
exothermic reactions.
Use only tubes that meet the criteria provided in this manual (see Tube Selection and Use, Chapter
3). Results may be affected if tubes are not within specifications. If tubes are of inadequate strength
so that tube breakage occurs, sample and test results will be lost, the rotor and distributor could be
damaged, and during required cleanup, contact with sharp glass fragments could result in personal
injury.
CAUTION
Do not operate Cellwasher 2 without a rotor properly installed on the drive shaft. See Rotor and
Distributor Installation, Chapter 2.
Do not operate the Cellwasher 2 with a rotor out of balance. To do so can cause damage to the
Cellwasher 2 drive assembly. See Rotor Loading and Balancing, Chapter 2.
We recommend using Coomb's control cells to confirm all negative results.
The Cellwasher 2 can be damaged if connected to the wrong voltage. Check the voltage before
plugging the instrument into a power source. Kendro is not responsible for incorrect installation.
Always maintain the Cellwasher 2 in the recommended manner. See Chapter 4, Maintenance.
!
Important Safety Information
!
iii

Table of Contents SORVALL®Centrifuges
Page
Safety Information Page . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . iii
Chapter 1. DESCRIPTION
Cellwasher 2 Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1
Applications Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1
Cellwasher 2 Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2
Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4
Chapter 2. INSTALLATION
Unpacking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1
Location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1
Environmental Conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2
Electrical Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2
Tubing Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2
Rotor and Distributor Installation . . . . . . . . . . . . . . . . . . . . . . 2-5
Rotor Loading and Balancing . . . . . . . . . . . . . . . . . . . . . . . . . 2-7
Chapter 3. OPERATION
Controls and Indicators . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-1
Principles of Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3
Tube Selection and Use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-5
Priming the System & Adjusting Saline Fill Volume . . . . . . . 3-6
Auto Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-8
High and Low Speed Operation . . . . . . . . . . . . . . . . . . . . . . . 3-10
Tube Breakage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-11
Momentary Power Interruption . . . . . . . . . . . . . . . . . . . . . . . . 3-12
Emergency Sample Recovery . . . . . . . . . . . . . . . . . . . . . . . . . 3-12
Chapter 4. MAINTENANCE
Preventive Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-1
Sevice Decontamination Policy . . . . . . . . . . . . . . . . . . . . . . . . 4-5
Parts Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-7
Tubing Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-7
Fuse Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-7
Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-7
iv
Table of Contents

Cellwasher 2 Table of Contents
APPENDIX
Warranty
Index
Decontamination Certificates
List of Illustrations
Figure Page
2-1. Cellwasher 2 Tubing Diagram . . . . . . . . . . . . . . . . . . 2-3
2-2. Stainless Steel Rotating Bowl Installation . . . . . . . . . 2-6
2-3. Distributor Installation . . . . . . . . . . . . . . . . . . . . . . . . 2-6
2-4. Tube Placement for Balancing Less Than 12 Tubes . 2-7
3-1. Cellwasher 2 Controls and Indicators . . . . . . . . . . . . 3-3
3-2. Location of Mechanical Override . . . . . . . . . . . . . . . 3-12
4-1. Collecting Ring Assembly, Exploded View . . . . . . . . 4-3
4-2. Location of Decant Coil and Drive Ring . . . . . . . . . . 4-10
4-3. Location of Lift Plate Ring . . . . . . . . . . . . . . . . . . . . 4-10
List of Tables
Table Page
1-1. Accessories Supplied . . . . . . . . . . . . . . . . . . . . . . . . . 1-4
2-1. Parts Location: Tubing Diagram . . . . . . . . . . . . . . . . 2-3
3-1. Cellwasher 2 Controls and Indicators . . . . . . . . . . . . 3-1
4-1. Troubleshooting Chart: Problem A . . . . . . . . . . . . . . 4-8
4-2. Troubleshooting Chart: Problem B . . . . . . . . . . . . . . 4-8
4-3. Troubleshooting Chart: Problem C . . . . . . . . . . . . . . 4-9
4-4. Troubleshooting Chart: Problem D . . . . . . . . . . . . . . 4-9
4-5. Applications Troubleshooting Chart:
False Negative Results . . . . . . . . . . . . . . . . . . . . . . . 4-11
4-6. Applications Troubleshooting Chart:
False Positive Results . . . . . . . . . . . . . . . . . . . . . . . . 4-12
4-7. Applications Troubleshooting Chart:
Inconsistent Reaction Strength. . . . . . . . . . . . . . . . . 4-13
v/vi

Cellwasher 2 Description
1-1
Chapter 1: DESCRIPTION
This manual contains information required to operate and maintain the SORVALL®Cellwasher 2 Cell
Washing System. If you require additional information regarding operation or maintenance, please contact
Kendro for assistance. In the United States, call Kendro toll-free 1-800-522-7746; outside the United
States, contact the nearest Kendro office (see back cover) or your local representative for SORVALL®
products. SORVALL®product information is available on our internet web site at http://www.kendro.com
or http://www.kendro.de.
* The Cellwasher 2 is not designed to automatically separate donor whole blood to create
the necessary 2 to 5% red blood cell/saline suspension. The Cellwasher 2 may, however,
be used as a centrifuge to manually separate red blood cells from whole blood.
Cellwasher 2 Description
The Cellwasher 2 is designed to perform the washing phase of the
Coomb's Procedure automatically. In normal AUTO mode opera-
tion, after placing two or three drops of a pre-prepared 2 to 5% red
blood cell/saline suspension* into each tube, the Cellwasher 2 will
automatically add saline, spin at high speed to concentrate the cells,
decant the saline, and agitate to resuspend the washed cells for the
addition of Coomb's reagent. The Cellwasher 2 is also capable of
being used manually as a low speed centrifuge, spinning at either of
two preset speed ranges (LOW speed or HIGH speed mode, see
Cellwasher 2 Specifications on next page).
The Cellwasher 2 combines a peristaltic saline pump and a wet
chamber centrifuge in one cabinet. The centrifuge portion uses a
dedicated DA-12 Rotor, which is a unique dual-angle swinging-
bucket rotor that is designed to accommodate up to 12 each of
either 3 ml or 5 ml tubes. The Cellwasher 2 is microprocessor-
controlled, featuring a front control panel with sensor-touch keys
and a digital timer for the wash cycle. Other features include a
saline-detect system with an audible low-saline warning, and an
agitate cycle that ensures complete resuspension of cells and
eliminates manual agitation.
Applications Information
The Cellwasher 2 is intended to be used in routine blood work to
speed up and simplify the procedures for determining human blood
groups and types. Often, the results of blood tests made using the
Cellwasher 2 determine donor/patient compatibility for blood trans-
fusions, and the results of these tests must be accurate to prevent
serious transfusion reactions. If testing procedures provided by the
reagent manufacturers are not followed exactly, or if test materials
are outdated, accurate test results cannot be guaranteed.

Description SORVALL®Centrifuges
1-2
False Coomb's readings can be caused by any of the following:
• Improper ratio of patient serum and cells.
• Incomplete washing of cells.
• Contaminated saline or supply tubing.
• Improper decanting of saline solutions.
• Inactive Coomb's serum.
• Failure to add Coomb's serum to a tube.
• Improper final centrifugation.
• Inaccurate reading or recording of results.
While the Cellwasher 2 provides accurate washing, decanting, and a
proper speed for the final spin, it does not eliminate all potential
errors. Proper laboratory procedures require adequate controls of all
tests performed with this instrument (read the WARNINGS). Refer to
Tables 4-5, 4-6, and 4-7 for applications troubleshooting informa-
tion. When using the Cellwasher 2, always follow standard labora-
tory procedures in handling and disposing of blood serum, and
observe safety precautions and good laboratory practices.
Cellwasher 2 Specifications
Dimensions:
Depth . . . . . . . . . . . . . . . . . . . . . . . . . . . .35.6 cm (14.0 inches)
Width . . . . . . . . . . . . . . . . . . . . . . . . . . . .31.8 cm (12.5 inches)
Height: Lid closed . . . . . . . . . . . . . . . . . .36.8 cm (14.5 inches)
Lid open . . . . . . . . . . . . . . . . . . .57.2 cm (22.5 inches)
Mass (Weight): . . . . . . . . . . . . . . . . . . . .16.4 kg (36.0 lbs)
Motor Type: . . . . . . . . . . . . . . . . . . . . . . .3-speed induction
Speeds:*
60 Hz Models: Low 1100 to 1200 rpm
High 3500 to 3600 rpm
Decant 550 to 600 rpm
50 Hz Models: Low 1400 to 1500 rpm
High 2900 to 3000 rpm
Decant 550 to 600 rpm
Length of Modes:
Automatic . . . . . . . . . . . . . . . . . . . . . . . . .Each multi-step wash cycle
is 80 seconds, except on
100V 50 Hz models, which
have 90-second cycles.
Manual . . . . . . . . . . . . . . . . . . . . . . . . . . .High or low, optional, up to
999 seconds if timed; high
speed indefinite if in HOLD
!
W A R N I N G
Werecommendusing
Coomb's control cells to confirm all
negativeresults.
* Speed in revolutions per minute (rpm) is related to angular velocity, ω , according to the
following:
ω = (rpm) = (rpm) (0.10472)
Where w= rad/s. All further references in this manual to speed will be designated as rpm.
2π
60
()
!
W A R N I N G
TheSORVALLCellwasher2
is intended to be used in strict accor-
dance with The American Association
of Blood Banks' guidelines defined in
the most recent edition of the AABB
TechnicalManual(publishedbyAABB
Press. Bethesda, MD).

Cellwasher 2 Description
1-3
Time Display Accuracy: ........................ ±3%(ManualMode*)
Noise Level: . . . . . . . . . . . . . . . . . . . . . .<60 dB**
Rotor Radii:
rmaximum 9.43 cm
raverage 6.015 cm
rminimum 2.60 cm
Relative Centrifugal Force:
60 Hz Models: Low at rmax 127 to 152 g
at ravg 81 to 97 g
at rmin 35 to 42 g
High at rmax 1290 to 1365 g
at ravg 823 to 871 g
at rmin 356 to 376 g
50 Hz Models: Low at rmax 206 to 237 g
at ravg 132 to 151 g
at rmin 57 to 65 g
High at rmax 886 to 948 g
at ravg 565 to 605 g
at rmin 244 to 261 g
Electrical Requirements:***
Electrical Supply . . . . . . . . . . . . . . . . . . . .120V ±10%. 60 Hz, 5A
100V ±10%. 60 Hz, 5A
100V ±10%. 50 Hz, 5A
230V ±10%. 50 Hz, 2.5A
Tube Requirements:
Tube Strength . . . . . . . . . . . . . . . . . . . . . .Centrifuge-rated to handle
at least 1365 gunsupported
(for 50 Hz operation, at
least 948 gunsupported)
TubeMaterial: . . . . . . . . . . . . . . . . . . . . . .Type 1, class A borosilicate
orequivalenthigh-strength
rolled glass (such as Pyrex®)
Tube Style: . . . . . . . . . . . . . . . . . . . . . . . . Round bottom, thick walled,
rimless (no flange or bead)
Tube Size: Volume (all places) . . . . . . . .5 ml or 3 ml****
Diameter . . . . . . . . . . . . . . .5 ml: 12 mm +0.0/–0.5
3 ml: 10 mm +0.0/–0.5
Length . . . . . . . . . . . . . . . . . 75 mm ±1.5
Wall Thickness . . . . . . . . . . . 0.9 mm ±0.1
!
W A R N I N G
Tubesmustbeabletowith-
stand up to 1365 g(948 gin 50 Hz
operation) when run at a 45° angle
withtubewalls point-loadedandother-
wise unsupported. Using tubes of in-
adequatestrengthcouldresultintube
breakage:sampleandtestresultswill
be lost, the rotor and distributor could
be damaged, and cleanup will be re-
quired.Duringcleanup,beawarethat
glass fragments are sharp, and con-
tact could result in personal injury.
* At the start of a Manual run, the timer function is delayed for several seconds before
beginning, so that timing occurs only when the rotor is spinning; the timer is inactive
during the initial aggitation step that occurs before the spin starts.
** The maximum noise output with the instrument running at HIGH speed, measured 3 feet
from the front panel at an approximated operator's height.
*** Electrical requirements are indicated on the rating plate on the back of the instrument.
**** A set of Adapter Clips (Catalog No. 04330, 12/pkg., supplied) is required when using
3 ml, 10 mm diameter tubes in the DA-12 Rotor.

Installation SORVALL®Centrifuges
Accessories
The accessories listed in Table 1-1 are supplied with all Cellwasher 2
models.
SORVALL®5 ml, 12 mm x 75 mm Pyrex®tubes (Catalog Number
03102, 50/box), and SORVALL®3 ml, 10 mm x 75 mm Pyrex®tubes
(Catalog Number 03100, 50/box) are not supplied, but are recom-
mended for use in the Cellwasher 2.
Table 1-1. Accessories Supplied
Catalog
Number Description
43260 Distributor Assembly – for 12 mm x 75 mm and
10 mm x 75 mm tubes
12977 Tubing Kit containing:
1-255 mm (10 inch) long frosted Pump Tubing
1-1650 mm (65 inch) long, large diameter (5/16 inch i.d.,
7/16 inch o.d.) clear Tygon®Tubing from which to cut:
1-Overflow Tubing 150 mm (6 inch) long
1-Discharge Tubing 200 mm (8 inch) long
1-Drain Tubing (cut desired length from remainder;
1220 mm [48 inch] supplied on new product)
1-2900 mm (114 inch) long, small diameter (1/4 inch i.d.,
3/8 inch o.d.) clear Tygon®Tubing from which to cut:
1-Flow Tubing 305 mm (12 inch) long
1-Vent Tubing 150 mm (6 inch) long
1-Supply Tubing (cut desired length from remainder;
610 mm [24 inch] supplied on new product)
1-Pump Connector
2-Y-Connectors
2-Adjustable Tubing Clamps
2-Nylon Cable Ties
Installation Instructions
12925 Flow Control Valve
04285 DA-12 Rotor (includes a set of 12 Adapter Clips for use
with 3 ml 10 mm x 75 mm tubes, Catalog No. 04330)
12796 Rotating Bowl Assembly
1-4

Cellwasher 2 Installation
Chapter 2: INSTALLATION
This chapter contains the information needed to unpack and install the SORVALL®Cellwasher 2.
Unpacking
As soon as you receive your Cellwasher 2, carefully inspect it for
any shipping damage that may have occurred. If you find any
damage, please report it immediately to the transportation company
and file a damage claim, then notify Kendro. Check all packages
received against the shipping list and, after unpacking, check the
accessories received against Table 1-1 on page 1-4; if any items are
missing, contact Kendro (see back cover).
Location
Place the Cellwasher 2 on a sturdy, level bench or table near a sink,
drain, or waste container that can receive the decanted saline. The
following factors should be considered when selecting a location:
• Allow a 150 mm (6 inch) clearance area behind the instrument
for tubing and a waste container. For safe operation, maintain a
50 mm (2 inch) "clearance envelope" on all sides.
Personnel should know that the Cellwasher 2 routinely deals
with significant energy levels and could move suddenly in the
unlikely event of a rotor failure. Laboratory management proce-
dures should require that no person or any hazardous materials
are within the clearance envelope while the Cellwasher 2 is
operating. During operation, personnel should be instructed not
to lean on or move the Cellwasher 2, not to stay within the
clearance envelope longer than necessary for operational rea-
sons, and not to deposit potentially hazardous materials within
the clearance envelope.
• Allow a 610 mm (24 inch) clearance above the tabletop or
bench surface for lid to open.
• Drainage in the Cellwasher 2 is accomplished by gravity, so
the discharge tubing must extend downward from the instru-
ment to drain, sink, or waste receptacle.
• The preferred location for the saline supply is either at or
above instrument level.
• To obtain best results, minimize tubing length from the
saline supply to the Cellwasher 2 and from the Cellwasher 2
to the drain, sink, or waste receptacle.
!
W A R N I N G
During operation, never
lean on or move the instrument, keep
the defined clearance area clear of
all objects (including all hazardous
and flammable materials), and do not
work within the clearance area.
!
C A U T I O N
Coolingairisdrawnintothe
Cellwasher 2 from all four sides of
the base. Be sure that the entrances
are not obstructed.
2-1

Installation SORVALL®Centrifuges
2-2
Environmental Conditions
An ambient temperature range of 10°C to 38°C (50°F to 100°F)
should be maintained for Cellwasher 2 operation. The relative hu-
midity can be up to 90%, non-condensing.
The Cellwasher 2 is intended for use in 1) a Pollution Degree 2
Environment, 2) an installation category II supply circuit, and 3) at a
maximum altitude of 2000 meters. The Cellwasher 2 is a Class A
product and is not intended for home use. If used in a domestic
environment, it may cause radio interference, in which case the user
may be required to take adequate measures.
Electrical Requirements
The Cellwasher 2 has a 3-wire (2-pole and earth) power cord with a
universal keyed plug that inserts into a receptacle at the back of the
instrument. The other end must be connected to the appropriate
power supply as specified below and on the rating plate on the back
of the instrument.
120V ±10%, 60 Hz, 5A; 100V ±10%, 60 Hz, 5A;
100V ±10%, 50 Hz, 5A; or 230V ±10%, 50 Hz, 2.5A
Cellwasher 2 120V and 100V power cords use a NEMA 5-15P
three-prong molded plug with a ground pin and parallel blades that
will fit Hubbell receptacle No. 5261 for 120 volts. 230V power cords
are CE-rated, and are supplied with a 3-pin plug that fits a 16A CEE-
17 single-phase receptacle (2-pole and earth).
The ON/OFF main power switch has a 3-Amp fuse; however, for
emergency disconnect purposes, we recommend a separate means of
power interruption in a remote location.
Tubing Installation
NOTE The overflow tubing serves as an emergency liquid exit
to protect instrument components in the event of tube
breakage or drain blockage. To ensure that potentially
biohazardous liquid does not exit into the work surface,
route overflow tubing to a 250 ml (or larger) open waste
container.
The Cellwasher 2 comes with the tubing already connected to the
instrument, but not installed in the peristaltic pump (do not open
the tubing replacement kit, save it for future use). To complete
the tubing installation, unwrap the bundle of tubing at the back of
the instrument and, referring to figure 2-1 and table 2-1, install
the tubing according to the procedure that follows.
!
W A R N I N G
Thereceptacleusedshould
be a grounded 3-wire system. If it is
not,theequipmentmustbegrounded
to reduce the possibility of electrical
shock.

Cellwasher 2 Installation
2-3
Table 2-1. Parts Location: Tubing Diagram
(keyed to figure 2-1)
Item Description
1* Overflow Tubing (from overflow tray), 150 mm (6 inch)
2 Vent Tubing, 150 mm (6 inch)
3 Pump Tubing, 255 mm (10 inch), frosted
4 Flow Tubing (to nozzle), 305 mm (12 inch)
5 Discharge Tubing (from collector ring), 200 mm (8 inch)
6 Supply Tubing, 610 mm (24 inch),
7 Drain Tubing 1220 mm (48 inch),
8 Pump Connector
9 Y-Connector
10 Adjustable Tubing Clamp
* In the event of saline residual in the rotor chamber (for example, from overfill of tubes or
tube breakage) saline will exit through this tube. Under normal operating conditions, saline
will exit through the drain tubing not through the overflow tube.
5
PUMP HOUSING
THUMBSCREW
6
10 8
7
9
10
2
1
9
WASTE
SALINE
4
3
FLOW
CONTROL
VALVE
Figure 2-1. Cellwasher 2 Tubing Diagram

Installation SORVALL®Centrifuges
2-4
1. Unscrew the four knurled thumbscrews from the peristaltic pump,
and remove the front half of the pump housing (do not remove
the pump roller assembly).
2. Turn the roller assembly so that one of the rollers is in the 11
o'clock position.
3. Position the 255 mm (10 inch) frosted pump tubing (with pump
connector) in front of the pump so that the connector is posi-
tioned just above the left side of the pump, then without turning
the roller assembly, route the pump tubing around the rollers.
4. Pull up on the two ends of the pump tubing to fit the tubing
between the pump housing and the lower roller.
NOTE Do not try to fit the pump tubing with a sharp object, this
could puncture the tubing.
5. With the pump tubing properly positioned in the back half of the
pump housing, reinstall the front half of the housing. Gently
stretch the ends of the pump tubing to eliminate slack while
fitting the front half of the housing in place.
6. Visually check to make sure that the front half of the housing is
properly aligned and fully seated against the back half (a gap
indicates that the pump tubing is pinched; if so, remove the front
half of the housing and repeat steps 3 through 6).
7. Install the four thumbscrews and hand-tighten.
8. Check the pump tubing installation by turning the roller assem-
bly shaft counterclockwise using pliers (the rollers should rotate
smoothly without pinching tubing).
9. If the 610 mm (24 inch) supply tubing is too long, cut it to an
appropriate length, then slide the flow control valve (catalog
number 12925, supplied) onto the supply tubing, and connect the
end of the supply tubing to a saline supply reservoir.
NOTE The preferred location for the saline supply is either at or
above instrument level. If, at some later time, the saline
reservoir is moved from its original location, recheck the
saline volume by priming the system as directed in
Chapter 3, Operation, Priming the System.
10. Make sure the drain tubing flows downward to an open sink,
drain, or waste receptacle because drainage is accomplished by
gravity. Use the shortest length of drain tubing possible (if the
1220 mm (48 inch) tubing is too long, cut it to an appropriate
length) and make sure the drain tubing is positioned so there is
no upward travel to result in a "trap" for collected waste fluid.
W A R N I N G
Saline solution from some
manufacturers contain sodium azide
as a preservative. If this solution is
discharged directly down the drain,
explosive azide salts may form as
the sodium azide reacts with the
plumbing.Checkwithyoursaline sup-
plier before discharging saline from
the Cellwasher 2 into a drain.
!

Cellwasher 2 Installation
2-5
11. Ideally, the drain tubing should be positioned so that it can not
become immersed in collected waste fluid. However, if there is a
possibility that the drain tubing will become immersed in waste
fluid, cut the drain tubing at a convenient point near the waste
receptacle and insert the Y-Connector. Always insert a Y-Con-
nector in the drain line when using a waste receptacle.
NOTE The Y-Connector prevents pressure from building up in
the drain tubing due to submerged tubing or air block-
age. Failure to vent the drain tubing by installing the Y-
Connector can result in improperly decanted samples.
12. If necessary, use additional tubing and connectors from the
Tubing Kit shipped with the Cellwasher 2 to supply saline and
discharge waste properly.
Rotor and Distributor Installation
a. DA-12 Dual Angle Rotor and Rotating
Bowl Installation
1. Open the lid of the Cellwasher 2.
NOTE There may be one or more holes in the stainless steel
rotating bowl. The holes are for balancing purposes only
and will not affect the performance of the rotating bowl.
2. Position the rotating bowl assembly (catalog number 12796,
supplied) over the motor drive shaft. Align the two drive pins
in the bowl with the slot in the drive shaft (see figure 2-2).
Slide the bowl down the drive shaft until the two pins in the
bowl engage the rubber drive ring. Press the bowl down
firmly.
3. Slide the DA-12 Rotor (catalog number 04285, supplied)
over the drive shaft and lower it gently into the bowl.
4. Hold the edge of the bowl and turn the rotor slowly until the
drive pins in the bowl engage the rotor and the rotor drops
down onto the bottom of the bowl.
b. Distributor Installation
Place the Distributor (catalog number 43260, supplied) over the
rotor knob (see figure 2-3). Turn the distributor until its three
pins engage the three holes in the rotor. Press the distributor
down until it is completely seated on the rotor.
C A U T I O N
If the rotating bowl assem-
bly, rotor, and distributor are not
installed properly, damage to the in-
strument could result. Before
operating the Cellwasher 2:
•Make sure the drive pins in rotat-
ing bowl are engaged in the rubber
drive ring and in the rotor.
•Make sure the distributor is firmly
seated on the rotor with the dis-
tributor pins completely engaged
in the rotor (see figure 2-3).
!

Installation SORVALL®Centrifuges
2-6
Figure 2-3. Distributor Installation
DISTRIBUTOR
CANNULA
DISTRIBUTOR
PIN
ROTOR
KNOB
DA-12
ROTOR
Figure 2-2. Stainless Steel Rotating Bowl Installation
MOTOR DRIVE
SHAFT
SLOT
ALIGNMENT
STAINLESS STEEL
ROTATING BOWL
ASSEMBLY
DRIVE PINS

Cellwasher 2 Installation
2-7/2-8
W A R N I N G
Results may be affected if
tubes are not within the specifica-
tions provided in Chapter 1 on page
1-3. Using tubes of inadequate
strength could result in tube break-
age: sample and test results will be
lost, the rotor and distributor could be
damaged, and cleanup will be re-
quired. During cleanup, contact with
glass fragments could result in per-
sonal injury.
!
Figure 2-4. Tube Placement for Balancing Less Than Twelve Tubes
EIGHT
Rotor Loading and Balancing
The Cellwasher 2 can process any balanced load up to twelve tubes.
When using less than a full complement of tubes, place the tubes in
opposing compartments to ensure that the rotor is symmetrically
balanced (see figure 2-4). The volume of saline delivered through
the distributor will be the same; at the locations without tubes, the
saline will be dispensed into the basin and drained off. Read the
WARNING.
TEN
NINE
SIX
FOUR
THREE
TWO
= TUBE
= EMPTY

Installation SORVALL®Centrifuges
2-8