Lambda OMNICOLL User manual

LAMBDA OMNICOLL
Fraction Collector and Sampler
Operation MANUAL
LAMBDA OMNICOLL
Fraction Collector - Sampler

LAMBDA OMNICOLL Fraction Collector - Sampler Operation MANUAL 2023.01
www.lambda-instruments.com 1
LAMBDA OMNICOLL Fraction Collector - Sampler
The LAMBDA OMNICOLL introduces new concepts in fraction collection and sampling. The
OMNICOLL fraction collector and sampler can be used for all kinds of chromatography. In
the number of simultaneous chromatographic streams, the OMNICOLL is not outperformed
by any other fraction collector on the market.
●Collects fractions in any racks of your choice
●Collects unlimited number of fractions in single or multiple fractions
●No danger of spilling; the complete collector is placed above the tubes
●Unlimited number of programs
●Extremely easy programming of rack and tube position by means of a simple pen
●Fractionation according to time (0.1-999.9 min and 1-9999 min) or volume (0.05-500
ml or 0.1-30 l) or drops (with optional drop-counter)
●Sampling with pause (0.1-9999 min) and line washing; the fraction collector can be
used for sampling during cell cultures, fermentations, chemical reactions, etc.
●Can be placed in a cold bath or any other thermo-stabilized container
●Solvent resistant metal construction with tubes easily accessible from all sides
●Multi-column attachment for simultaneous fraction collection (optional)
●Low voltage power supply –battery operation possible for field-applications)
●RS-232 interface (optional)
LAMBDA Laboratory Instruments
is developer and producer of special laboratory instruments mainly for biotechnology,
microbiology, food and agricultural, chemical and pharmaceutical research and development
as well as for general laboratory and research applications.
LAMBDA MINIFOR –highly innovative and compact fermenter/bioreactor system for
laboratory scale fermentation and cell cultures
LAMBDA OMNICOLL –fraction collector-sampler for unlimited number of fractions
LAMBDA PRECIFLOW, MULTIFLOW, HIFLOW, MAXIFLOW & MEGAFLOW peristaltic
pumps –reliable, precise and extremely compact
LAMBDA SAFETY POWDER DOSER / Hi-DOSER –allows automatic feeding of powders
without spoon. Safe operation with hazardous material (GLP)
LAMBDA VIT-FIT / VIT-FIT HP polyvalent syringe pump with extremely robust mechanics –
programmable infusion and filling from micro syringes to large volume syringes of 150 ml
without adapter
LAMBDA MASSFLOW –precise gas flow measurement and control with data acquisition
option
LAMBDA PUMP-FLOW INTEGRATOR –with LAMBDA pumps and doser allows the
visualization and recording of the pumped volume

LAMBDA OMNICOLL Fraction Collector - Sampler Operation MANUAL 2023.01
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TABLE OF CONTENTS
1Setting up the Fraction Collector 3
1.1 Assembling the fraction collector.................................................................................3
1.2 Assembly of fraction collection tubing (single stream)................................................5
1.3 How to increase the capacity of the fraction collector?...............................................6
1.4 Multi-channel fraction collection –multiple stream sampling .....................................7
1.4.1 Standard multi-stream fraction collection................................................................7
1.4.1.1 Mounting of the standard front multiple stream adapter 7
1.4.2 Multi-stream fraction collection VERSION 2022 .....................................................9
1.4.2.1 Mounting #6950: mobile multi-stream collection adjustable up to 32 channels 10
1.5 Connecting the drop-counter detector (optional)......................................................12
2Start of the fraction collection 13
3Programming of the fraction collector 14
3.1 Principle of programming...........................................................................................14
3.2 Ready to use Fraction collector with the supplied tube racks ..................................15
3.3 Programming the OMNICOLL for any tube racks or recipients................................16
3.3.1 Coding X-axis for tube distance in row 16
3.3.2 Coding Y-axis for row distance 17
3.4 Control Panel of OMNICOLL Fraction Collector.......................................................17
3.4.1 Selection of linear, meander-like or row collection 18
3.4.2 Time resolution (0.1 or 1 minute) 19
3.4.3 Drops and count divider (Division co-efficient) 20
3.4.4 Address selection (for PC control) 21
3.5 Fraction collection according to the volume..............................................................21
3.6 Calibration of the peristaltic pump and the OMNICOLL ...........................................22
3.7 Fraction collection with a time interval in-between fractions (“high”)........................23
4Remote controls 25
4.1 Collection of single samples......................................................................................25
4.2 Collection of multiple samples...................................................................................25
5Practical advice 26
6Maintenance 26
7For your safety 26
8Technical specifications 27
8.1 General specification.................................................................................................27
8.2 Inputs/outputs............................................................................................................28
9ACCESSORIES AND SPARE PARTS 28
9.1 List of accessories and spare parts...........................................................................28
10 Guarantee 30
11 Appendix 31
11.1 RS communication protocol for LAMBDA OMNICOLL fraction collector-sampler31
11.1.1 Format of data sent by the PC to the fraction collector and back 31
11.1.2 Commands 31
11.1.3 Checksum control 32
11.1.4 Format of the data transmission 33
11.2 How to set the OMNICOLL Fraction Collector-Sampler address?.......................33
11.3 RS-connection scheme..........................................................................................33

LAMBDA OMNICOLL Fraction Collector - Sampler Operation MANUAL 2023.01
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1 SETTING UP THE FRACTION COLLECTOR
1.1 Assembling the fraction collector
The setup of the fraction collection - sampler, LAMBDA OMNICOLL is very easy - a short
video of the installation can be found at www.youtube.com/watch?v=33J9U_2-b-o
Figure 1.1-1 Push the first tub-sheet into the
slots on one of the shorter sides of the yellow
support.
Figure 1.1-2 Push the second tub-sheet into the
slots on another shorter side of the yellow
support.
Figure 1.1-3 Insert the black frame into the
corresponding slots of the yellow supports
Figure 1.1-4 Pay attention to proceed at the
same speed on both sides otherwise it will end
up in blocking. The tub-sheet should not slip out
of the slots.
Figure 1.1-5 Fix both frames in position with
four bolts.
Figure 1.1-6 Tighten it securely with the help of
the provided hexagonal key.

LAMBDA OMNICOLL Fraction Collector - Sampler Operation MANUAL 2023.01
www.lambda-instruments.com 4
Figure 1.1-7 Push the metal rod through both
fixation nuts on the right side of the frame and
fasten them with screws. If needed fix the
support plate on the metal.
Figure 1.1-8 Place the fixing mat in the support.
When needed clean the mat from dust and dirt
with a humid cloth. Make sure that the mat is dry
before use.
Figure 1.1-9 Place the control unit just at the
beginning of the gear rails.
The distance from the edge should be the same
on the left and right sides. The gear wheels of
the control unit must engage correctly in the
gears of both rails.
Be sure that nothing will disturb the movement
of the control unit during fractionation.
Figure 1.1-10 Connect the cable of the control
unit of the LAMBDA OMNICOLL fraction
collector and sampler with the cable of the
power supply plug.
Figure 1.1-11 Place the supplied magnet on the
right inner side of the frame to stop the fraction
collection.
Figure 1.1-12 Place the tube rack on the fixing
mat, so that the first tube is just under the tip of
the tubing and the edge of the rack is parallel to
the yellow frontal support.

LAMBDA OMNICOLL Fraction Collector - Sampler Operation MANUAL 2023.01
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1.2 Assembly of fraction collection tubing (single stream)
(a) PTFE tubing (external diameter 1.8 mm)
(b) Fixing nut
(c) O-ring (1 x 1.5 mm)
(d) Tubing guide
Figure 1.2-1 Place the magnetic tubing holder
conveniently in the center of the fraction collector
carriage and control unit.
Figure 1.2-2 Push the delivered PTFE tubing through
the pipe guide as shown.
Figure 1.2-3 Slide the O-ring on the PTFE tubing.
Figure 1.2-4 Push the tubing through the tubing
guide and make sure a few mm (≈ 5mm) of PTFE
tubing reach out of the tubing guide so that the drops
form only at the tip of PTFE tubing.
Figure 1.2-5 Screw the pipe guide onto the tubing
guide to such an extent that the PTFE tubing does
not move freely inside the tubing guide.
Do not screw more strongly than necessary!
Figure 1.2-6 Let the PTFE tubing pass through the
magnetic tubing holder. Fix the tubing guide, with the
help of the adjusting screw in the holder, on the
moving arm of the fraction collector at a convenient
distance above tubes.

LAMBDA OMNICOLL Fraction Collector - Sampler Operation MANUAL 2023.01
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1.3 How to increase the capacity of the fraction collector?
Since several lower parts of the collector can be coupled together, the capacity of the
collector can be increased many times.
The control unit can move freely from one lower unit to the other on the gear rails. Only two
rectangular coupling bars are needed to keep two units together.
•Remove the two blind bars from the rear side of the frame of the first lower part unit
and the front side of the second lower part unit. Insert the coupling bars (Art. No.
6912) on their place and fix with the four bolts.
•Be aware that you must remove the fixed stop signal from the first frame unit and
place it onto the next one. You may also use the magnetic stop contact.
•Make sure that nothing will block the movement of the control unit during the fraction
collection.
Figure 1.3-1 Fraction collector with extended
capacity for 12 streams and 250 ml fraction
collection bottles.
The tube capacity of the LAMBDA OMNICOLL fraction collector and sampler can be easily
increased by the addition of lower support. Virtually any number of capacity extensions can
be added.

LAMBDA OMNICOLL Fraction Collector - Sampler Operation MANUAL 2023.01
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1.4 Multi-channel fraction collection –multiple stream sampling
An interesting property of the LAMBDA OMNICOLL fraction collector is the option to collect
simultaneously the streams of many chromatographic columns.
For LAMBDA OMNICOLL multi-stream fraction collectors, different designs exist:
•Standard multi-stream
•Version 2022 multi-stream
1.4.1 Standard multi-stream fraction collection
The standard multi-stream assembly is available for 2 to 18 or even more effluents of
multiple chromatography columns. This multi-stream assembly is technically very simple and
polyvalent. It allows an easy adaptation of various experimental needs directly by the user.
Examples of the standard multi-stream fraction collection:
Figure 1.4.1-1 Example of
taking 4x2 fractions in a 4-
channel configuration
Figure 1.4.1-2 Example of
taking 6x2 fractions in a 6-
channel configuration
Figure 1.4.1-3 Example of
taking 20 simultaneous
fractions in a 20-stream
(channel) configuration
1.4.1.1 Mounting of the standard front multiple stream adapter
Figure 1.4.1-4 Unscrew the tubing guide holder
screw on the moving arm of the OMNICOLL
fraction collector
Figure 1.4.1-5 Place the bar holder on the
tubing guide holder screw.

LAMBDA OMNICOLL Fraction Collector - Sampler Operation MANUAL 2023.01
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Figure 1.4.1-6 Insert the rectangular bar into the
bar holder as shown.
Figure 1.4.1-7 Slide the right end of the bar
through the opening on the guide plate.
Figure 1.4.1-8 Screw the bar holder on the
moving arm of the fraction collector with the
corresponding socket key (hex-key).
Use e.g., a thin spatula to push the screw
through the moving arm.
Figure 1.4.1-9 Slide the tubing guide into the
tubing guide holder, so that a convenient height
above the tubes is obtained.
Fix the tubing guide to the holder using the
corresponding socket key (hex-key).
Figure 1.4.1-10 Place the tubing guide holder at
the desired fraction position and secure it with
the socket head screw using the corresponding
socket key (Allen key).
Figure 1.4.1-11 Place the tubing guide holder in
position above the first fraction of each stream
(channel).
If 4 streams are collected in a row, the place:
first tubing guide over tube no. 1,
second tubing guide over tube no. 4,
third tubing guide over tube no. 7 and
fourth tubing guide over tube no. 10
For more information about the multiple stream mounting, please do not hesitate to contact
us [e-mail].

LAMBDA OMNICOLL Fraction Collector - Sampler Operation MANUAL 2023.01
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1.4.2 Multi-stream fraction collection VERSION 2022
LAMBDA OMNICOLL with accessories for multi-stream fraction collector VERSION 2022 can
be carried out from 2 to 64 simultaneous threads.
LAMBDA OMNICOLL accessories for multi-stream fraction collection VERSION 2022 are:
•art. no. 6952
Accessory for fixed multi-stream collection adjustable up to 64 channels
•art. no. 6908-n
Tube guide, 100 mm
•art. no. 6950
Accessory for mobile multi-stream collection adjustable up to 32 channels
•art. no. 6951
Accessory for mobile multi-row collection adjustable up to 8 channels
Examples of customized multi-stream fraction collector LAMBDA OMNICOLL Version 2022:
Figure 1.4.2-1 Front side of the customized
multi-channel fraction collector LAMBDA
OMNICOLL for 18 streams.
(Accessory for fixed multi-stream collection
adjustable up to 64 channels art. no. 6952).
Figure 1.4.2-2 Back side of the customized
multi-channel fraction collector LAMBDA
OMNICOLL for 18 streams.
(Accessory for fixed multi-stream collection
adjustable up to 64 channels art. no. 6952).
Figure 1.4.2-3 Configuration for collecting
simultaneous streams into 96-well plates
(art. no. 6951 - Accessory for mobile multi-row
collection adjustable up to 8 channels)
Figure 1.4.2-4 Customized multi-channel
fraction collector LAMBDA OMNICOLL for 6
streams (art. no. 6950 - Accessory for mobile
multi-stream collection adjustable up to 32
channels).