Lambda OMNICOLL User manual

LAMBDA OMNICOLL
Fraction Collector and Sampler
Operation MANUAL
LAMBDA OMNICOLL
Fraction Collector - Sampler

LAMBDA OMNICOLL Fraction Collector - Sampler Operation MANUAL 2023.01
www.lambda-instruments.com 1
LAMBDA OMNICOLL Fraction Collector - Sampler
The LAMBDA OMNICOLL introduces new concepts in fraction collection and sampling. The
OMNICOLL fraction collector and sampler can be used for all kinds of chromatography. In
the number of simultaneous chromatographic streams, the OMNICOLL is not outperformed
by any other fraction collector on the market.
●Collects fractions in any racks of your choice
●Collects unlimited number of fractions in single or multiple fractions
●No danger of spilling; the complete collector is placed above the tubes
●Unlimited number of programs
●Extremely easy programming of rack and tube position by means of a simple pen
●Fractionation according to time (0.1-999.9 min and 1-9999 min) or volume (0.05-500
ml or 0.1-30 l) or drops (with optional drop-counter)
●Sampling with pause (0.1-9999 min) and line washing; the fraction collector can be
used for sampling during cell cultures, fermentations, chemical reactions, etc.
●Can be placed in a cold bath or any other thermo-stabilized container
●Solvent resistant metal construction with tubes easily accessible from all sides
●Multi-column attachment for simultaneous fraction collection (optional)
●Low voltage power supply –battery operation possible for field-applications)
●RS-232 interface (optional)
LAMBDA Laboratory Instruments
is developer and producer of special laboratory instruments mainly for biotechnology,
microbiology, food and agricultural, chemical and pharmaceutical research and development
as well as for general laboratory and research applications.
LAMBDA MINIFOR –highly innovative and compact fermenter/bioreactor system for
laboratory scale fermentation and cell cultures
LAMBDA OMNICOLL –fraction collector-sampler for unlimited number of fractions
LAMBDA PRECIFLOW, MULTIFLOW, HIFLOW, MAXIFLOW & MEGAFLOW peristaltic
pumps –reliable, precise and extremely compact
LAMBDA SAFETY POWDER DOSER / Hi-DOSER –allows automatic feeding of powders
without spoon. Safe operation with hazardous material (GLP)
LAMBDA VIT-FIT / VIT-FIT HP polyvalent syringe pump with extremely robust mechanics –
programmable infusion and filling from micro syringes to large volume syringes of 150 ml
without adapter
LAMBDA MASSFLOW –precise gas flow measurement and control with data acquisition
option
LAMBDA PUMP-FLOW INTEGRATOR –with LAMBDA pumps and doser allows the
visualization and recording of the pumped volume

LAMBDA OMNICOLL Fraction Collector - Sampler Operation MANUAL 2023.01
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TABLE OF CONTENTS
1Setting up the Fraction Collector 3
1.1 Assembling the fraction collector.................................................................................3
1.2 Assembly of fraction collection tubing (single stream)................................................5
1.3 How to increase the capacity of the fraction collector?...............................................6
1.4 Multi-channel fraction collection –multiple stream sampling .....................................7
1.4.1 Standard multi-stream fraction collection................................................................7
1.4.1.1 Mounting of the standard front multiple stream adapter 7
1.4.2 Multi-stream fraction collection VERSION 2022 .....................................................9
1.4.2.1 Mounting #6950: mobile multi-stream collection adjustable up to 32 channels 10
1.5 Connecting the drop-counter detector (optional)......................................................12
2Start of the fraction collection 13
3Programming of the fraction collector 14
3.1 Principle of programming...........................................................................................14
3.2 Ready to use Fraction collector with the supplied tube racks ..................................15
3.3 Programming the OMNICOLL for any tube racks or recipients................................16
3.3.1 Coding X-axis for tube distance in row 16
3.3.2 Coding Y-axis for row distance 17
3.4 Control Panel of OMNICOLL Fraction Collector.......................................................17
3.4.1 Selection of linear, meander-like or row collection 18
3.4.2 Time resolution (0.1 or 1 minute) 19
3.4.3 Drops and count divider (Division co-efficient) 20
3.4.4 Address selection (for PC control) 21
3.5 Fraction collection according to the volume..............................................................21
3.6 Calibration of the peristaltic pump and the OMNICOLL ...........................................22
3.7 Fraction collection with a time interval in-between fractions (“high”)........................23
4Remote controls 25
4.1 Collection of single samples......................................................................................25
4.2 Collection of multiple samples...................................................................................25
5Practical advice 26
6Maintenance 26
7For your safety 26
8Technical specifications 27
8.1 General specification.................................................................................................27
8.2 Inputs/outputs............................................................................................................28
9ACCESSORIES AND SPARE PARTS 28
9.1 List of accessories and spare parts...........................................................................28
10 Guarantee 30
11 Appendix 31
11.1 RS communication protocol for LAMBDA OMNICOLL fraction collector-sampler31
11.1.1 Format of data sent by the PC to the fraction collector and back 31
11.1.2 Commands 31
11.1.3 Checksum control 32
11.1.4 Format of the data transmission 33
11.2 How to set the OMNICOLL Fraction Collector-Sampler address?.......................33
11.3 RS-connection scheme..........................................................................................33

LAMBDA OMNICOLL Fraction Collector - Sampler Operation MANUAL 2023.01
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1 SETTING UP THE FRACTION COLLECTOR
1.1 Assembling the fraction collector
The setup of the fraction collection - sampler, LAMBDA OMNICOLL is very easy - a short
video of the installation can be found at www.youtube.com/watch?v=33J9U_2-b-o
Figure 1.1-1 Push the first tub-sheet into the
slots on one of the shorter sides of the yellow
support.
Figure 1.1-2 Push the second tub-sheet into the
slots on another shorter side of the yellow
support.
Figure 1.1-3 Insert the black frame into the
corresponding slots of the yellow supports
Figure 1.1-4 Pay attention to proceed at the
same speed on both sides otherwise it will end
up in blocking. The tub-sheet should not slip out
of the slots.
Figure 1.1-5 Fix both frames in position with
four bolts.
Figure 1.1-6 Tighten it securely with the help of
the provided hexagonal key.

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Figure 1.1-7 Push the metal rod through both
fixation nuts on the right side of the frame and
fasten them with screws. If needed fix the
support plate on the metal.
Figure 1.1-8 Place the fixing mat in the support.
When needed clean the mat from dust and dirt
with a humid cloth. Make sure that the mat is dry
before use.
Figure 1.1-9 Place the control unit just at the
beginning of the gear rails.
The distance from the edge should be the same
on the left and right sides. The gear wheels of
the control unit must engage correctly in the
gears of both rails.
Be sure that nothing will disturb the movement
of the control unit during fractionation.
Figure 1.1-10 Connect the cable of the control
unit of the LAMBDA OMNICOLL fraction
collector and sampler with the cable of the
power supply plug.
Figure 1.1-11 Place the supplied magnet on the
right inner side of the frame to stop the fraction
collection.
Figure 1.1-12 Place the tube rack on the fixing
mat, so that the first tube is just under the tip of
the tubing and the edge of the rack is parallel to
the yellow frontal support.

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1.2 Assembly of fraction collection tubing (single stream)
(a) PTFE tubing (external diameter 1.8 mm)
(b) Fixing nut
(c) O-ring (1 x 1.5 mm)
(d) Tubing guide
Figure 1.2-1 Place the magnetic tubing holder
conveniently in the center of the fraction collector
carriage and control unit.
Figure 1.2-2 Push the delivered PTFE tubing through
the pipe guide as shown.
Figure 1.2-3 Slide the O-ring on the PTFE tubing.
Figure 1.2-4 Push the tubing through the tubing
guide and make sure a few mm (≈ 5mm) of PTFE
tubing reach out of the tubing guide so that the drops
form only at the tip of PTFE tubing.
Figure 1.2-5 Screw the pipe guide onto the tubing
guide to such an extent that the PTFE tubing does
not move freely inside the tubing guide.
Do not screw more strongly than necessary!
Figure 1.2-6 Let the PTFE tubing pass through the
magnetic tubing holder. Fix the tubing guide, with the
help of the adjusting screw in the holder, on the
moving arm of the fraction collector at a convenient
distance above tubes.

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1.3 How to increase the capacity of the fraction collector?
Since several lower parts of the collector can be coupled together, the capacity of the
collector can be increased many times.
The control unit can move freely from one lower unit to the other on the gear rails. Only two
rectangular coupling bars are needed to keep two units together.
•Remove the two blind bars from the rear side of the frame of the first lower part unit
and the front side of the second lower part unit. Insert the coupling bars (Art. No.
6912) on their place and fix with the four bolts.
•Be aware that you must remove the fixed stop signal from the first frame unit and
place it onto the next one. You may also use the magnetic stop contact.
•Make sure that nothing will block the movement of the control unit during the fraction
collection.
Figure 1.3-1 Fraction collector with extended
capacity for 12 streams and 250 ml fraction
collection bottles.
The tube capacity of the LAMBDA OMNICOLL fraction collector and sampler can be easily
increased by the addition of lower support. Virtually any number of capacity extensions can
be added.

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1.4 Multi-channel fraction collection –multiple stream sampling
An interesting property of the LAMBDA OMNICOLL fraction collector is the option to collect
simultaneously the streams of many chromatographic columns.
For LAMBDA OMNICOLL multi-stream fraction collectors, different designs exist:
•Standard multi-stream
•Version 2022 multi-stream
1.4.1 Standard multi-stream fraction collection
The standard multi-stream assembly is available for 2 to 18 or even more effluents of
multiple chromatography columns. This multi-stream assembly is technically very simple and
polyvalent. It allows an easy adaptation of various experimental needs directly by the user.
Examples of the standard multi-stream fraction collection:
Figure 1.4.1-1 Example of
taking 4x2 fractions in a 4-
channel configuration
Figure 1.4.1-2 Example of
taking 6x2 fractions in a 6-
channel configuration
Figure 1.4.1-3 Example of
taking 20 simultaneous
fractions in a 20-stream
(channel) configuration
1.4.1.1 Mounting of the standard front multiple stream adapter
Figure 1.4.1-4 Unscrew the tubing guide holder
screw on the moving arm of the OMNICOLL
fraction collector
Figure 1.4.1-5 Place the bar holder on the
tubing guide holder screw.

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Figure 1.4.1-6 Insert the rectangular bar into the
bar holder as shown.
Figure 1.4.1-7 Slide the right end of the bar
through the opening on the guide plate.
Figure 1.4.1-8 Screw the bar holder on the
moving arm of the fraction collector with the
corresponding socket key (hex-key).
Use e.g., a thin spatula to push the screw
through the moving arm.
Figure 1.4.1-9 Slide the tubing guide into the
tubing guide holder, so that a convenient height
above the tubes is obtained.
Fix the tubing guide to the holder using the
corresponding socket key (hex-key).
Figure 1.4.1-10 Place the tubing guide holder at
the desired fraction position and secure it with
the socket head screw using the corresponding
socket key (Allen key).
Figure 1.4.1-11 Place the tubing guide holder in
position above the first fraction of each stream
(channel).
If 4 streams are collected in a row, the place:
first tubing guide over tube no. 1,
second tubing guide over tube no. 4,
third tubing guide over tube no. 7 and
fourth tubing guide over tube no. 10
For more information about the multiple stream mounting, please do not hesitate to contact
us [e-mail].

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1.4.2 Multi-stream fraction collection VERSION 2022
LAMBDA OMNICOLL with accessories for multi-stream fraction collector VERSION 2022 can
be carried out from 2 to 64 simultaneous threads.
LAMBDA OMNICOLL accessories for multi-stream fraction collection VERSION 2022 are:
•art. no. 6952
Accessory for fixed multi-stream collection adjustable up to 64 channels
•art. no. 6908-n
Tube guide, 100 mm
•art. no. 6950
Accessory for mobile multi-stream collection adjustable up to 32 channels
•art. no. 6951
Accessory for mobile multi-row collection adjustable up to 8 channels
Examples of customized multi-stream fraction collector LAMBDA OMNICOLL Version 2022:
Figure 1.4.2-1 Front side of the customized
multi-channel fraction collector LAMBDA
OMNICOLL for 18 streams.
(Accessory for fixed multi-stream collection
adjustable up to 64 channels art. no. 6952).
Figure 1.4.2-2 Back side of the customized
multi-channel fraction collector LAMBDA
OMNICOLL for 18 streams.
(Accessory for fixed multi-stream collection
adjustable up to 64 channels art. no. 6952).
Figure 1.4.2-3 Configuration for collecting
simultaneous streams into 96-well plates
(art. no. 6951 - Accessory for mobile multi-row
collection adjustable up to 8 channels)
Figure 1.4.2-4 Customized multi-channel
fraction collector LAMBDA OMNICOLL for 6
streams (art. no. 6950 - Accessory for mobile
multi-stream collection adjustable up to 32
channels).

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1.4.2.1 Mounting #6950: mobile multi-stream collection adjustable up to 32
channels
Figure 1.4.2-5 First you need to assemble the
moving arm holder.
Figure 1.4.2-6 The holder is fixed with two
screws using a hexagonal key.
Figure 1.4.2-7 The moving arm holder attaches
to the black rectangle attached to the device.
Figure 1.4.2-8 The holder is fixed with one
screw using a hexagonal key.
Figure 1.4.2-9 The moving arm is mounted on
the holder.
Figure 1.4.2-10 The moving arm is fixed with
one washer and bolt.

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Figure 1.4.2-11 The tube guide is mounted on
a moving arm in accordance with the selected
position.
(For each stream one tube guide (art. no. 6950)
is needed).
Figure 1.4.2-12 The tube guide is fixed with
one bolt.
Figure 1.4.2-13 The tube guide can be raised or
lowered with a hexagonal key.

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1.5 Connecting the drop-counter detector (optional)
The drop counter is an optional device which could be obtained on request.
Fractionation can also be done according to the desired number of drops in the fraction
collector using the drop-counter.
Figure 1.5-1 Showing drop counter (detector)
cable with the communication module (Art. No.:
6929)
Figure 1.5-2 Connect the drop-counter
(detector) cable to the “DROP” socket of the
communication module.
Figure 1.5-3 *Partially unscrew the tubing guide
holder screw (A), so that the tubing guide (B) can be
rotated. Slightly loosen the nut (C), so that the PTFE
tubing (D) can move freely inside the tubing guide
(B). *Screw the drop counter (E) on the lower end of
the tubing guide (B). *Tighten the tubing guide holder
screw (A) until the tubing guide (B) does not move
freely. Securely tighten the nut (C), to prevent the
movement of PTFE tubing (D) inside the tubing guide
(B). *Fix the drop counter cable (F) to the tubing
guide (B) with the help of Teflon or a spiral wrap, etc.
Let the drop-counter cable (F) pass together with the
PTFE tubing (D) through the magnetic holder (H).
Figure 1.5-4 Connect the power supply to the
“POWER” socket in the communication module.
Figure 1.5-5 Connect the OMNICOLL fraction
collector to the “OMNICOLL” socket in the
communication module.
Figure 1.5-6 If required, the LAMBDA pump or
valve to the “PUMP/VALVE” socket in the
communication module.

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2 START OF THE FRACTION COLLECTION
✔Place the control unit just at the beginning of the gear rails. (Figure 1.1-9) The
distance from the edge should be the same on the left and right sides. The gear
wheels of the control unit must engage correctly in the gears of both rails. Be sure
that nothing will disturb the movement of the control unit during fraction collection.
✔Connect the cable of the control unit of the OMNICOLL fraction collector and sampler
with the power supply plug. (Figure 1.1-10)
✔Connect the power supply to the AC mains (95-240 V / 50-60 Hz). The control unit
and its arm move automatically to the first position in the first row.
✔Press the SET button (a short acoustic signal is heard). By pressing the
COUNT/TIME button you can select fractionation according to time or volume. The
yellow LED indicates which collection mode has been selected. (Refer section 3.4.1).
✔Use the four buttons Λ Λ Λ Λ under the display to select the desired value. Confirm it
by pressing the SET button again (a long acoustical signal is heard). The fraction time
can be set from 0.1 min to 999.9 min (about 16.6 hours) in 0.1 min steps or from 1 to
9999 min in 1 min steps. To change the time resolution, please refer to section 3.4.2.
For the setting of the volume, please refer to section 3.5.
✔Pass the dispensing tubing (PTFE) through the tubing holder as describe in section
1.2 and tighten the tube holder in the collector arm so that the dispensing tubing is
about 1 cm above the tubes or recipients.
✔Place the tube rack on the fixing mat (Figure 1.1-8), so that the first tube is just under
the tip of the tubing and the edge of the rack is parallel to the yellow frontal support
(Figure 1.1-12). Place the additional tube racks immediately behind the first and
following racks.
✔Make sure that nothing will block the movement of the control unit during the fraction
collection and that the tubing length is sufficient for taking all fractions or samples.
✔Press the START button to begin the collection of fractions or sample taking (Figure
3.6-6). The green LED will be on and, if you use a LAMBDA peristaltic pump
(PRECIFLOW, MULTIFLOW, HIFLOW or MAXIFLOW), it will be activated
automatically. (refer section 3.6)
✔You may use the magnetic block to stop the fraction collection. Place the supplied
magnet on the right inner side of the frame (Figure 1.1-11)
It might happen that the position of the optical sensors lies outside the coding
lines of the magnetic band inserted into the carriage.
When started in such a position, the fraction collecting arm will move either to
the extreme left or right position, according to the instrument setting and is
blocked there.
After several seconds an automatic protection will switch off the motor.
To move the arm into the coding mark signals just press the button STEP. The
arm will move to the border coding line and will operate correctly within the
marked limits of the inserted magnetic coded band.
If the moving arm is blocked in its movement or operated without inserted
magnetic coding band, the motor will stop and the message “Eror” will appear
on the display. Switch off the power, resolve the problem and start again as
usual.

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3 PROGRAMMING OF THE FRACTION COLLECTOR
A short video on programming the OMNICOLL fraction collector can be found at
www.lambda-instruments.com/fraction-collector/ - video
3.1 Principle of programming
Laboratory practice shows that programming of instruments equipped with microprocessors
is not simple and mistakes are easily made, especially when such instruments are used
occasionally.
We have developed a new method, which should eliminate such problems and make the
programming easy for all types of tube racks.
Figure 3.1-1 Magnetic coding band used for the
fraction positioning.
Figure 3.1-2 The fraction positions are indicated
by white lines drawn on the black magnetic
coding band as shown.
The white lines should be about 2 mm broad and full-length. These lines are detected by the
photo-detectors of the OMNICOLL fraction collector and the fraction collector stops at these
positions. The actual fraction position is about 1 mm after the first edge of the white lines.
Figure 3.1-3 The beginning and the end of a
row are indicated by short lines (about half the
length of the normal stripes or less and ~5 mm
broad) made on the lower edge (for the
beginning) and upper edge (for the end) of the
coding band, respectively.
Figure 3.1-4 The distance between the fraction
rows is programmed in a similar way. If you wish
to switch off the collector automatically after the
last row, draw the last line broader (approx. 1
cm)

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The beginning and end signals (at Figure 3.1-3) determine at the same time the position
of the first and the last fraction in the row. The fraction position is about 2 mm after the
first edge of the white line of the beginning and end stripes.
This simple coding principle allows the use of any racks and fraction stands
or other recipients with the LAMBDA OMNICOLL fraction collector and
sampler.
3.2 Ready to use Fraction collector with the supplied tube racks
The magnetic coding bands programmed with white lines for the desired tube racks are
provided with the OMNICOLL Fraction collector.
Figure 3.2-1 Place the longer and narrower
coding band (Y-axis) on the left inner metal
frame just under the gear-bar as shown.
The first coding line should lie approximately
10cm from the front frame.
Figure 3.2-2 Pull the X-axis coding band holder
from the OMNICOLL control unit as shown.
Figure 3.2-3 Place the shorter and broader
magnetic coding band into the X-axis holder.
Figure 3.2-4 Insert the X-axis holder with coding
band into the desired slot of the control unit, with
the white coding stripes facing up.
Place the magnetic coding strip strictly horizontally into the corresponding
slot! Otherwise, the optical detectors could be damaged.

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3.3 Programming the OMNICOLL for any tube racks or recipients
3.3.1 Coding X-axis for tube distance in row
Measure the distance between the tube centres of the desired rack and choose the number
of fractions in a row.
Figure 3.3-1 Place the coding bands on the
tube rack and mark the fractioning position with
a normal pencil.
Figure 3.3-2 Draw the white lines correctly on
the pre-marked places. Reserve approximately
2cm of the coding band for the beginning and
end-position signals.
Figure 3.3-3 Mark the beginning and end
signals on the lower and upper edge of the
coding band respectively.
Leave a minimum of about 6mm free space
between the first and last line of the row and the
beginning and end signals.
Figure 3.3-4 Place the coding band in the X-axis
holder and insert into the desired slot of the
control unit, with white stripes facing up.
The beginning and end signals at the lower or upper edge of the coding
band informs the microprocessor whether the arm of the control unit is on
right or left.

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3.3.2 Coding Y-axis for row distance
Measure the distance between rows. When several tube racks are used, pay attention to the
difference in distance between the last row of the first rack and the first row of the second
rack.
Figure 3.3-5 Mark the row distance with a pencil
on the coding band for Y-axis and draw the
white lines with the provided correction pen.
Figure 3.3-6 Place the coding band on the left
internal side of the metal frame just under the
gear bar.
If the fraction collection should be stopped at the last row, then make the last line broader
(approximately 1 cm thick). This functions as the stop signal.
Since the coding band is kept in place by magnetic force, it can be easily positioned.
When the control unit is placed in the starting position on the front of the
frame, the photoelectric detector on the left side of the control unit must be
situated before the first line. Otherwise the control unit will stop at the
position of the next row.
The first row line of the Y-axis should be ca 10 cm from the front.
3.4 Control Panel of OMNICOLL Fraction Collector

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3.4.1 Selection of linear, meander-like or row collection
The LAMBDA OMNICOLL fraction collector-sampler allows the following operation modes:
Line:
The fractions are collected from the left to the right. After the last fraction
of the row, the collecting arm moves to the left to the first position of the
next row.
Meander:
The fractions are taken alternatively from the left to the right and in the
following row from the right to the left and so forth.
Row:
The collecting arm does not move within the row. The collector moves
only from row to row. This collection mode is mainly used in the
simultaneous (multi-channel) fraction collection mode.
Figure 3.4-1 Press the button LINE
continuously while plugging the power supply
into the mains.
Figure 3.4-2 “rouu”for row mode of collection
appear on the display. Press the SELECTION
(below COUNT / TIME) button to select the
desired mode of collection.
Figure 3.4-3 “Mean”for meander-like mode of
collection appear on the display.
Figure 3.4-4 “Line” for linear mode of collection
appear on the display.
Figure 3.4-5 Confirm the selected mode of
collection by pressing SET button
In the row mode, the position of the fraction collecting arm can be adjusted by using the
STEP button.

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A small difference in the fraction positions with respect to the tube centres
may occur between fractions taken in opposite collection directions. If the
fraction lines are not too broad (about 2 mm), this difference in position is of
approx. 1 mm
3.4.2 Time resolution (0.1 or 1 minute)
The time resolution of the OMNICOLL fraction collector can be selected in the following way:
Figure 3.4-6 Press the button COUNT/TIME
while connecting the power supply to the mains.
Figure 3.4-7 The actual time resolution will
appear on the display “0.1M” or “1M”.
Figure 3.4-8 By pressing the button COUNT /
TIME below the COUNT and TIME LEDs you
can select to use 0.1 minute steps “0.1M” or 1
minute steps “1M”.
Figure 3.4-9 Confirm the choice by pressing the
button SET.