Life technologies novex PowerEase 500 User manual

USER GUIDE
For Research Use Only. Not for use in diagnostic procedures.
PowerEase®500 Power Supply
A programmable power supply for electrophoresis
Catalog Numbers EI8600, EI8700, and EI8675
Document Part Number A12162
Publication Number MAN0000738
Revision 9.0

Table of Contents
Warning...................................................................................................................................................................4
Kit Contents............................................................................................................................................................5
Product Specifications...........................................................................................................................................6
Front and Rear View of PowerEase®500............................................................................................................7
Introduction..........................................................................................................................................................10
Description of PowerEase®500 ..........................................................................................................................10
Methods ....................................................................................................................... 12
Getting Started .....................................................................................................................................................12
Operational Modes ..............................................................................................................................................13
Using PowerEase®500.........................................................................................................................................14
Printing..................................................................................................................................................................21
Custom Methods..................................................................................................................................................22
Troubleshooting...................................................................................................................................................24
Appendix...................................................................................................................... 25
Instrument Symbols and Safety.........................................................................................................................25
Consignes De Securite.........................................................................................................................................26
Sicherheits Anweisungen ...................................................................................................................................27
Run Conditions for Gels .....................................................................................................................................28
Repair, Maintenance, and Cleaning ..................................................................................................................29
Background Information on Voltage, Current, and Resistance.....................................................................31
Accessory Products..............................................................................................................................................32
Technical Support ................................................................................................................................................33
3

Warning
Federal
Communications
Commission
Advisory
This equipment has been tested and found to compl
y
with the limits for a Class
A digital device, pursuant to part 15 of the FCC rules. These limits are designed
to provide reasonable protection against harmful interference when the
equipment is operated in a commercial environment. This equipment
generates, uses, and can radiate radio frequency energy and, if not installed and
used in accordance with the instruction manual, may cause harmful
interference to radio communications. Operation of this equipment in a
residential area is likely to cause harmful interference in which case the user
will be required to correct the interference at their expense.
Changes or modifications not expressly approved by the party responsible for
compliance could void the user’s authority to operate the equipment.
4

Kit Contents
Types of Kits This manual is supplied with the followin
g
kits:
Kit Catalog No.
PowerEase®500 Power Supply (100/120 Vac 50/60 Hz) EI8600
PowerEase®500 Power Supply (220/240 Vac 50/60 Hz) EI8700
PowerEase®500 Pre-Cast Gel System
includes PowerEase®500, Temperature Monitoring Probe,
XCell
SureLock
™Mini-Cell and XCell II™Blot Module
EI8675
Kit Components PowerEase®500 Power Suppl
y
contains:
PowerEase®500 Power Supply 1 each
Instruction Manual 1 each
Extra Fuses 2 each
Power Cord (U.S., Canada, Europe, Taiwan and Japan) 1 each
Warranty Card 1 each
Temperature Probe (Optional) 1 each
See page 6for specifications and detailed description of PowerEase®500 Power
Supply.
Note To ensure safe, reliable operation, alwa
y
s operate the PowerEase®500 Power
Supply in accordance with the manufacturer’s instructions. Always wear
protective gloves and safety glasses when working in a laboratory environment.
See safety information on pages 25–27.
Warranty information is provided on page 33.
5

Product Specifications
Specifications Input Powe
r
100–120 VAC: 100–120V, 50/60 Hz, 1.2 A at maximum load
Fuse: One 1A/250V/AGC fast blow fuse
220–240 VAC: 220–240VAC, 50/60 Hz, 0.5 A at maximum load
Fuses: Two 1A/250V/5 × 20 mm fast blow fuses
Output Range 1–500 V (minimum step size 1 V)
1–500 mA (minimum step size 1 mA)
0.1–50 W (minimum step size 0.1 W)
Automatic crossover on reaching set limits.
Accuracy Voltage: ± 2% or ± 2 volts
Current: ± 2% or ± 2 mA
Wattage ± 2% or ± 0.2 watt
Drift < 1% in 8 hours after 30 minute warm-up
with constant supply voltage
Circuit Protection Open Circuit; Short Circuit; Thermal Protection
Over Voltage, Current, Wattage
Safety Load Detection
Thermal Shutoff (with optional temperature probe)
Isolated Floating Ground
Memory Control Non-volatile, up to 24 hours
Custom Methods Stored to RAM
Computer Interface RS232C
Display LCD, view area 114 x 64 mm
240 × 128 dots
Dimensions 16.5 cm (w) × 18 cm (h) × 20.5 cm (d)
Weight 2.9 kg (6.4 lbs)
Operating Ambient temperature 4–30°C, ≤80% relative
Environmental humidity, altitude < 2000 meters, indoor use only,
Conditions pollution degree 2
6

Front and Rear View of PowerEase®500
Front View of
PowerEase®500
+
TEMP
P
o
w
e
r
E
a
s
e
5
0
0
SELECT
S
T
O
P
S
T
A
R
T
POWER HIGH VOLTAGE
1
7
4
3
5
2
6
8
1. LCD Screen
2. Power Output Jacks, Positive (Red)
3. Power Output Jacks, Negative (Black)
4. Temperature Probe Jack
5. Functional Keys (Power, Start, Stop)
6. Directional Keys (Up, Down, Left, Right)
7. High Voltage LED
8. Power LED
Continued on next page
7

Front and Rear View of PowerEase®500, Continued
Rear View of
PowerEase®500
PRI TER
RS232
Fan
RS232C
Communi-
cation
Port
Printer
Port
Power Entry Module
with On/Off
main power switch
Continued on next page
8

Front and Rear View of PowerEase®500, Continued
Key Pad
Power button secondary On/Off switch for the high voltage components, fan, and LCD
screen.
Start button used to start a selected method program.
Stop button used to end the selected method and to backup to a previous screen.
SELECT
Select button used to select a menu choice and to move forward to the next applicable
screen.
Left directional button for moving left around the menu.
Right directional button for moving right around the menu.
Up directional button for moving up around the menu and to adjust numerical and
character values (higher).
Down direction button for moving down around the menu and to adjust numerical and
character values (lower).
9

Introduction
Description of PowerEase®500
Product
Description
The PowerEase®500 Power Suppl
y
is a microprocessor-controlled power suppl
y
for electrophoresis of pre-cast and hand-poured mini-gels. The power supply is
designed to simplify electrophoresis by combining high performance and
programming flexibility with ease of use when running mini-gel and blotting
applications.
The PowerEase®500 Power Supply is designed around the single gel concept:
you need to think only about the electrical parameters and limits for a single gel.
This manual describes the setup and operation of PowerEase®500 Power Supply
including important information on safety and maintaining the unit.
Features of
PowerEase®500
The important features of PowerEase®500 Power Suppl
y
are:
•Large LCD display allows electrical parameters to be displayed in either
graphical or numerical formats
•Eight pre-programmed methods for running and blotting Novex®pre-cast
mini-gels
•Four custom methods for running your custom applications
•Simple three step operating procedure to start running or blotting Novex®
pre-cast gels
•Microprocessor control provides highly accurate electrical outputs resulting
in exceptional blotting capability
•Parallel port for printing experimental results to most printers
•Optional Temperature Probe to continuously monitor the buffer temperature
and shut the unit down in the event of overheating
•Two sets of output jacks allows electrophoresis of multiple mini-cell units in
constant voltage, current, or watts
Continued on next page
10