
Black Level
The black level output is determined by this control.
When set to MAX, the output video resides on a standard
50mV black pedestal. The BLACK LEVEL control range
allows blacks to be pulled down so that whites can be contrast
enhanced and stretched. Using this mode with the GAIN
control in AUTO stretches the portion of the signal that is of
interest. When using external gating, the BLACK LEVEL
control is useful for removing unwanted light offsets due to
effects such as lens scattering and low level background light.
Microscope Use
A. Initial Set-up:
1. Set switches on rear of camera head as follows:
SHUTTER to OFF, FIELD/FRAME to FRAME.
2. Use the TEST signal to adjust the associated equipment
as outlined in the “Test” section on the previous page.
B. All Forms of Transmitted Light:
1. Set GAIN to AUTO and BLACK LEVEL to MAX.
2. Adjust light so white highlights are just under saturation.
3. Turn BLACK LEVEL control for the proper contrast.
C. Live or Real-time Fluorescence:
1. Set for best visual fluorescence using eyepieces.
2. Set GAIN to AUTO and BLACK LEVEL to MAX.
3. Turn BLACK LEVEL control for best black background.
D. Gated low Light Level Fluorescence:
1. Follow steps 1-3 in part C above for live fluorescence.
2. Set GAIN to MAN and control to MIN.
3. Select Gate (Integration) time and initiate.
Note: Increasing MAN GAIN can shorten the Gate time.
However, this may cause the image to be very noisy.