PerkinElmer 200 Series User manual

Series 200 Oven Family
User’s Manual

Release History
Part Number Release Publication Date
09936398 CFebruary 2002
Any comments about the documentation for this product should be addressed to:
User Assistance
PerkinElmer
710 Bridgeport Avenue
Shelton, Connecticut 06484-4794
U.S.A.
Or emailed to: [email protected]
Notices
The information contained in this document is subject to change without notice.
Except as specifically set forth in its terms and conditions of sale, PerkinElmer makes
no warranty of any kind with regard to this document, including, but not limited to, the
implied warranties of merchantability and fitness for a particular purpose.
PerkinElmer shall not be liable for errors contained herein for incidental consequential
damages in connection with furnishing, performance or use of this material.
Copyright Information
This document contains proprietary information that is protected by copyright.
All rights are reserved. No part of this publication may be reproduced in any form
whatsoever or translated into any language without the prior, written permission of
PerkinElmer, Inc.
Copyright © 2003PerkinElmer, Inc.
Produced in the USA.
Trademarks
Registered names, trademarks, etc. used in this document, even when not specifically
marked as such, are protected by law.
PerkinElmer is a registered trademark of PerkinElmer, Inc.

Contents
-i-
Safety and Regulatory Information
Electromagnetic Compatibility (EMC).......................................... S-1
United States .......................................................................... S-1
Addresses ....................................................................................... S-1
Conventions used in this Manual.................................................. S-2
Instrument Symbols and Warnings .............................................. S-2
Symbols ................................................................................... S-2
Warnings on the Oven ............................................................ S-4
Electrical Warnings ....................................................................... S-6
Quality Control/Good Laboratory Practices.................................. S-7
Quality Control ....................................................................... S-7
Certificate of System Control ................................................. S-7
Instrument Performance Verification (IPV) .......................... S-7
Routine Inspection and Suitability Test ................................ S-7
Hazardous Chemicals.................................................................... S-8
Definitions in Warning for Hazardous Chemicals................. S-8
Temperature, Humidity and Environment................................... S-9
Storage Conditions.................................................................. S-9
Introduction
About the Manual.......................................................................... 1-1
Chapter Content ..................................................................... 1-1
Introducing the Series 200 Peltier Column Oven......................... 1-2
About the Oven Controls, Indicators, and Connections ........ 1-5
Warranty Exclusions and Limitations.......................................... 1-7
Maintenance .................................................................................. 1-7
Warnings and Precautions ............................................................ 1-7
Solvents................................................................................... 1-7
Buffers..................................................................................... 1-8
Corrosion................................................................................. 1-8
Air Bubbles ............................................................................. 1-8
Pressure Buildup .................................................................... 1-9
Specifications ................................................................................. 1-10

Contents
-ii -
Installing the Oven
Prepare Your Laboratory............................................................... 2-1
Required Air Quality .............................................................. 2-1
Sufficient Electrical Power..................................................... 2-2
Space Requirements ............................................................... 2-2
Solvents/Mobile Phase............................................................ 2-2
Unpack Your Series 200 Peltier Column Oven ............................ 2-3
Electrical Requirements and Settings .......................................... 2-3
Oven Power Cord and Fuses .................................................. 2-4
AC Line Cord .......................................................................... 2-5
Connect the Tubing ....................................................................... 2-6
Turn-On the Oven.......................................................................... 2-7
Using the Column Oven
Operating Overview....................................................................... 3-1
Operating Summary...................................................................... 3-2
Shutting Down the System ........................................................... 3-2
Appendix
Series 200 Accessory Control Installation Guide

Illustrations
-i-
Safety and Regulatory Information
Figure S-1. Location of side panel warning labels on
the Peltier column oven ................................................................ S-4
Figure S-2. Location of rear panel warning labels on
the Peltier column oven ................................................................ S-5
Introduction
Figure 1-1. Front View of the Series 200 Peltier column oven....... 1-3
Figure 1-2. Front view of the column chamber showing
the column inlet and outlet fittings.............................................. 1-4
Figure 1-3. Peltier Column Oven controller ................................... 1-5
Figure 1-4. Back panel of the Peltier Column Oven ...................... 1-6

Illustrations
-ii -
Installing the Oven
Figure 2-1. Rear panel of the oven .................................................. 2-4
Figure 2-2. Removing and installing fuses ..................................... 2-5
Figure 2-3. AC outlet configurations............................................... 2-6
Figure 2-4. Peltier Column Oven in a typical LC ssystem.............. 2-7
Using the Column Oven
Figure 3-1. Peltier Column Oven controller ................................... 3-2

Safety and Regulatory
Information
Electromagnetic Compatibility (EMC)
United States
This product is classified as a digital device used exclusively as industrial,
commercial, or medical test equipment. It is exempt from the technical
standards specified in Part 15 of the FCC Rules and Regulations, based on
Section 15.103 (c).
Addresses
To obtain assistance with the Series 200 Peltier Column Oven, contact the
Company as described below:
User Assistance
PerkinElmer
710 Bridgeport Avenue
Shelton, Connecticut 06484-4794
Tel: [phone]
Fax: [phone]
Supplies, accessories, and replacement parts can be ordered directly from
PerkinElmer using the eight-digit part numbers provided in the manual. To
place an order or ask for information, call your local PerkinElmer representative.

S-2Safety and Regulatory Information Conventions used in this Manual
Conventions used in this Manual
The following graphic symbols and special text formats are used in this
manual to identify important safety information:
A warning indicates an operation that could cause
personal injury if precautions are not followed.
A caution indicates an operation that could cause
instrument damage if precautions are not followed.
Note Notes emphasize significant information in a
procedure or description.
Instrument Symbols and Warnings
Symbols
The following three types of warning symbols appear on the instrument:
This symbol indicates an operation (or instrument
condition) that could cause personal injury if
precautions are not followed. This can be any type of
hazard.
When you see this symbol, refer to the safety pages in
the manual for further information.
Consulter les documents d’accompagnement.
This symbol indicates the danger of electric shock if
precautions are not followed due to the presence of high
voltage.
Attention. Risque de choc électrique.

Safety and Regulatory Information Instrument Symbols and Warnings S-3
This symbol indicates the danger of burns if
precautions are not followed due to the presence of
heat in this instrument area. This may also indicate a
hot surface that can be easily touched.
AVERTISSEMENT. Surface chaude.
The following additional graphic symbols used on the instrument:

S-4Safety and Regulatory Information Instrument Symbols and Warnings
Warnings on the Oven
The following warning labels are affixed to the side and rear panels of the
column oven: See Figures S-1 and S-2.
30.0
3 1.0
Figure S-1. Location of side panel warning labels on the Series 200 Peltier
Column Oven.

Safety and Regulatory Information Instrument Symbols and Warnings S-5
Figure S-2. Location of the rear panel warning labels on the Series 200
Peltier Column Oven.

S-6Safety and Regulatory Information Electrical Warnings
The following information is printed on a warning label that covers the
fuse receptacle on the rear panel of the oven:
The Series 200 Peltier Column Oven is shipped from
the factory with:
-The operating voltage set to 230 VAC.
-The main AC power switch set to the OFF
position.
-1.0 amp fuses installed.
If your line voltage is 115 volts, you need to switch the
voltage selector setting to 115 and install the 2.0 amp
fuses before installing the line cord and turning on the
AC power.
Electrical Warnings
Electrical Hazard: Connect the instrument to an AC
line power outlet that has a protective ground
connection. To ensure satisfactory and safe operation
of the instrument, it is essential that the protective
ground conductor (the green/yellow lead) of the line
power cord be connected to a true electrical ground.
Any interruption of the protective ground conductor,
inside or outside the instrument or disconnection of
the protective ground terminal may impair the
protection provided by the instrument.
Electrical Hazard: Do not operate the instrument with
any covers or parts removed.
Electrical Hazard: Do not make adjustments,
replacements or repairs to this instrument except as
described in the User Documentation. Only a
PerkinElmer service representative or similarly
trained and authorized person should service the
instrument.
Electrical Hazard: Use only fuses of the specified type
and with the required current rating for replacement.

Safety and Regulatory Information Quality Control/Good Laboratory Practices S-7
Quality Control/Good Laboratory Practices
Quality Control
The user should develop appropriate quality control procedures for the LC
Peltier Column Oven (and the entire LC system) to ensure suitability for its
intended use. These procedures typically consist of periodic performance
verifications and routine inspections and suitability tests.
Certificate of System Control
Each Series 200 Peltier Column Oven is carefully built and tested in a
controlled system in accordance with the requirements specified in its
applicable PerkinElmer Final Assembly and Test Specification.
Each instrument is certified to meet its functional and performance
specification upon release to shipment. The integrity of this quality system is
routinely audited and is certified by the British Standards Institution as
meeting all requirements of ISO 9001:1987 (Certification No. FM 22179).
Instrument Performance Verification (IPV)
To maintain functional performance, PerkinElmer recommends a yearly
Instrument Performance Verification (IPV) of the Series 200 Peltier Column
Oven by a PerkinElmer Service Engineer to ensure its operation within
published specifications. These tests consist of measuring the most
important Peltier Column Oven characteristics, such as retention time
repeatability. Certification is available for regulatory compliance. Contact
your local Perkin-Elmer Sales and Service office.
Routine Inspection and Suitability Test
To ensure that the LC system is operating within established criteria (for
example, peak resolution, peak asymmetry, precision, retention time, column
plate count, pressure limits, signal/noise ratio, etc.), perform a system
suitability test (which closely resembles the intended assay) prior to running
a sample analysis with the Series 200 Peltier Column Oven in the LC system.
While the Universal Test Mix (UTM) P/N 0089-0893 can be used for a
system check, we recommend that you develop a separate system suitability
test and acceptance criteria for each of your assays.

S-8Safety and Regulatory Information Hazardous Chemicals
Hazardous Chemicals
Before using mobile phase solvents, you should be thoroughly familiar
with all hazards and safe handling practices. Observe the solvent
manufacturer’s recommendations for use, storage, and disposal. These
recommendations are normally provided in the material safety data sheets
(MSDS) supplied with the solvents.
Some chemicals used with this instrument may be
hazardous or may become hazardous after completion of an
analysis. The responsible body (for example, the Lab
Manager) must take the necessary precautions to ensure
that the surrounding workplace and that the instrument
operators are not exposed to hazardous levels of toxic
substances (chemical or biological) as defined in the
applicable Material Safety Data Sheets (MSDS) or OSHA,
ACGIH, or COSHH documents. Venting for fumes and
disposal of waste must be in accordance with all national,
state and local health and safety regulations and laws.
Definitions in Warning for Hazardous Chemicals
Responsible body: “Individual or group responsible for the use and
maintenance of equipment, and for ensuring that operators are adequately
trained.” [per IEC 1010-1, Amendment 2 ].
Operator: “Person operating equipment for its intended purpose.” [per IEC
1010-1, Amendment 2 ].
OSHA: Occupational Safety and Health Administration (United States)
ACGIH: American Conference of Governmental Industrial Hygienists
COSHH: Control of Substances Hazardous to Health (United Kingdom)

Safety and Regulatory Information Temperature, Humidity and Environment S-9
Temperature, Humidity and Environment
This instrument is designed for indoor use only.
This instrument is not designed for operation in an
explosive environment.
Safe Operation
This instrument will operate safely between +5°C and +40°C (40°F and
104°F) with an ambient relative humidity of between 20 and 80%
noncondensing
Installation Category
This instrument is able to withstand transient overvoltage according to
Installation Category II as defined in IEC 1010-1.
Pollution Degree
This equipment will operate safely in environments that contain
nonconductive foreign matter up to Pollution Degree 2 in IEC 1010-1.
Storage Conditions
The Series 200 Peltier Column Oven may be stored under the following
conditions:
•Ambient temperature is -20°C to +60°C (−68°F to +140°F)
•Ambient relative humidity is 20% to 80% noncondensing
•Altitude is in the range 0 to 12,000 m.

Introduction
About the Manual........................................................................................... 1-1
Chapter Content....................................................................................... 1-1
Introducing the Series 200 Peltier Column Oven ......................................... 1-2
About the Oven Controls, Indicators, and Connections .......................... 1-5
Warranty Exclusions and Limitations .......................................................... 1-7
Maintenance................................................................................................... 1-7
Warnings and Precautions............................................................................. 1-7
Solvents..................................................................................................... 1-7
Buffers....................................................................................................... 1-8
Corrosion................................................................................................... 1-8
Air Bubbles ............................................................................................... 1-8
Pressure Buildup...................................................................................... 1-9
Specifications.................................................................................................1-10

Introduction
About the Manual
This manual contains important information regarding potential hazards
that may arise during the operation of the Peltier column oven. All
references, except where indicated in this manual, that refer to the Series
200 Peltier Column Oven also apply to the Series 200 Column Oven. It is
essential that the Safety and Regulatory Information preceding this chapter
be read and thoroughly understood by all potential users of the oven.
The Peltier column oven should be used according to
the instructions provided in the manual. If used
otherwise, the protection provided by the Peltier
column oven may be impaired.
Chapter Content
In the first chapter of this manual we describe the Series 200 Peltier
column oven and its controls. In the second chapter we describe installation
of the oven in your LC system. Electrical requirements and tubing
connections are also included in Chapter 2.
In the third chapter we include principles of operation for the oven,
routine operating procedures, and the procedure for shutting down the
system. This manual is written for a person who has developed a working
knowledge of liquid chromatography and understands:
•The function of each major component in a liquid chromatography
system.
•How to select a column based on the analysis you wish to perform.
•The principles of solvent selection and sample preparation.

1-2Introduction Introducing the Series 200 Peltier Column Oven
If you require an introduction to these concepts, the following book is an
excellent reference: Practical Liquid Chromatography -- An Introduction by
R.W. Yost, L.S. Ettre, and R.D. Conlon, published by The Perkin-Elmer
Corporation (1980), P/N 0993-9656.
Introducing the Series 200 Peltier Column Oven
The Series 200 Peltier Column Oven controls the column temperature;
variations in column temperature can cause significant changes in retention
times. The oven provides controlled temperatures which aid analyses by
decreasing mobile phase viscosity and increasing mass transfer and sample
solubility. The oven also provides cooling, i.e. the capability of controlling
the oven temperature in the 10 to 25 °C region.
The major features of the Peltier Column Oven include the following:
•A low-dispersion heat exchanger which heats the mobile phase and
sample before it enters the column. The injected sample remains a plug
as it passes through the heat exchanger, which prohibits peak
broadening. Because of this solvent preheating technique, the mobile
phase encounters a stationary phase of uniform temperature over the
entire length of the column.
•An open-bed design which eliminates the need for specific blocks or
inserts for different column sizes. Up to three 3/8-in.×30 cm or three
¼-in.×30 cm HPLC columns can be easily placed inside the column oven.
The column oven chamber is large enough to accommodate a guard
column and an analytical column for precise temperature control.
•The Peltier Column Oven can be operated either manually or remotely
via an RS-232C port.
•A leakage sensor is included in the column oven. If a leak occurs, a red
light on the front panel illuminates. This feature protects against
potential hazards and oven damage.
•The anodized aluminum column chamber (see the top illustration in
Figure 1-1) is corrosion resistant. It is light, strong, and enhances
temperature uniformity.