Rigaku Phoenix HT User manual

Phoenix HT
User Manual
May 2012
401718 Rev A
TM

Phoenix HT User Manual
© 2007–2012 Rigaku Automation, Inc. ii 401718 Rev A
Copyright
Copyright © 2007-2012 Rigaku Corporation and/or Rigaku Americas Corporation (Rigaku). All rights
reserved.
No part of this publication may be stored in a retrieval system, transmitted or reproduced in any way,
including but not limited to photocopy, photography, magnetic or other record without prior written permission
of Rigaku. To order additional copies of this publication, request this manual part number (shown at the top of
this page) from your authorized Rigaku service center.
This publication may contain or reference information concerning or related to products protected by either
copyrights or patents (or both) and does not convey any license under the patent rights of Rigaku nor the
rights of others. Rigaku does not assume any liabilities arising out of any infringements of patents or other
rights of third parties.
Disclaimer
Rigaku reserves the right to change its products and services at any time to incorporate technological
developments. This manual is subject to change without prior notice as part of a continuous product
development. Although this manual has been prepared with every precaution to ensure accuracy, Rigaku
assumes no liability for any errors or omissions, nor for any damages resulting from the application or use of
this information. Except as specifically set forth in its terms and conditions of sale, Rigaku makes no warranty
of any kind with regard to this document, including, but not limited to, the implied warranties of merchantability
and fitness for a particular purpose. Rigaku shall not be liable for errors contained herein for incidental
consequential damages in connection with furnishing, performance or use of this material. This manual
supersedes all previous editions.
Rigaku shall not be liable for any damages whatsoever arising out of the use or misuse of the product(s)
covered by this document.
Trademarks
Phoenix DT, Phoenix HT, Minstrel, Gallery, CrystalTrak, and CrystalMation are trademarks of Rigaku
Automation, Inc. All other trademarks and registered trademarks are sole property of their respective owners.
Document
Part Description: Phoenix HT User Manual
Part Number: 401718
Revision Letter: A
Publication Date: 05-30-2012
Any comments about the documentation for this product should be emailed to: [email protected] or
addressed to:
Rigaku Americas Corporation
Attn: MARCOM Group
9009 New Trails Drive
The Woodlands, TX 77381 U.S.A.
Software
ARI Phoenix Version: 1.10.3.2
CrystalTrak Version 2.3.37 (DB v3.0.2)
Authorized EU Representative:
Rigaku Europe
Unit B6 Chaucer Business Park, Watery Lane,, Kemsing, SEVENOAKS, Kent TN15 6QY
011 44 1732 763367

© 2007–2012 Rigaku Automation, Inc. iii 401718 Rev A
Table of Contents
Preface ........................................................................................................................v
CHAPTER 1. INTRODUCTION ........................................................................................1
1.1. General Description ...................................................................................... 1
1.2. Phoenix HT System Major Components ......................................................... 2
1.2.1 Phoenix Base System .............................................................................. 2
1.2.2 Enclosure Status Light Indicators ............................................................ 3
1.2.3 Protein Chiller Block ............................................................................... 3
1.2.4 Wash Stations ......................................................................................... 5
1.2.5 Nano-Dispense Controller ...................................................................... 6
1.2.6 Anti-Static Bar ........................................................................................ 7
1.2.7 Active Nest ............................................................................................. 7
1.2.8 Plate and Shelf Sensors ........................................................................... 8
1.2.9 Active Nest Terminals ............................................................................. 8
1.3. Safety Features ............................................................................................. 8
1.4. Potential Hazards .......................................................................................... 9
1.4.1 Networking ............................................................................................ 9
1.4.2 Electric Power Hazards ........................................................................... 9
1.4.3 Moving the Phoenix HT System from Cold to Warm Areas ....................... 9
1.5. Rigaku Service and Support .......................................................................... 9
CHAPTER 2. BASICS ..................................................................................................11
2.1. System Power-On ....................................................................................... 11
2.2. Shutting Down the System ......................................................................... 12
2.3. Running the System .................................................................................... 12
2.4. Status Light Indicators ................................................................................ 12
2.5. Using the Stop, Start, and Emergency Stop (EMO) Pushbuttons .................... 13
2.6. Resetting the System after an Emergency Motor Off (EMO) Shutdown .......... 13
CHAPTER 3. QUICK START PROCEDURES ....................................................................14
3.1. Preliminary Setup ....................................................................................... 14
3.2. Phoenix HT Software Procedures ................................................................. 15
CHAPTER 4. PHOENIX HT APPLICATION REFERENCE ....................................................19
4.1. Organization ............................................................................................... 19
4.2. File Menu .................................................................................................... 20
4.3. View Menu .................................................................................................. 20
4.4. Jobs Menu ................................................................................................... 20
4.5. Tools Menu ................................................................................................. 21
4.6. Help Menu .................................................................................................. 23
4.7. Views Pane and Icons .................................................................................. 23
4.8. Jobs View .................................................................................................... 23
4.9. Manage Device View ................................................................................... 24
4.9.1 Chilled Block and Ambient Block Tabs .................................................. 24
4.9.2 Onboard Plates Tab .............................................................................. 25
4.10. Job History View ......................................................................................... 26

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© 2007–2012 Rigaku Automation, Inc. iv 401718 Rev A
CHAPTER 5. MAINTENANCE .......................................................................................28
5.1. Scheduled Maintenance .............................................................................. 28
5.2. Equipment Cleaning .................................................................................... 28
5.3. Daily Detergent Wash Required ................................................................... 28
5.4. Replacing the Nano-Tip Valve Harness Assembly ........................................ 30
5.4.1 Removing the Nano-Tip Valve Harness ................................................. 30
5.4.2 Installing the Replacement Nano Valve Harness .................................... 33
5.5. Calibrating the Nano Dispenser .................................................................. 35
5.6. Aligning the Nano Dispenser ....................................................................... 37
Options and Spare Parts ............................................................................................39
Facilities Layout and Cabling .....................................................................................40
Revision History ........................................................................................................48
Index ........................................................................................................................49

© 2007–2011 Rigaku Automation, Inc. v401718 Rev A
Preface
The Preface provides documentation conventions, icons, and terminology used throughout this
manual.
Warning and Caution Notices
This manual uses the icons and typographic conventions described and illustrated here.
WARNING
Warnings alert you to situations that could result in serious personal injury or loss of
life.
AVERTISSEMENT
Les avertissements vous alertent aux situations qui pourraient avoir comme
conséquence des blessures ou perte ou vie sérieuse.
WARNING
WARNING alarmieren Sie zu den Situationen, die ernste Personenschäden oder Verlust
oder Lebensdauer ergeben konnten.
ALERTA
Las alertas le alertan a las situaciones que podrían dar lugar a daños corporales o a
pérdida o a vida seria.
CAUTION
Cautions indicate situations that can damage the machine.
ATTENTION
Les attentions indiquent les situations qui peUV ent endommager la machine.
VORSICHT
Vorsicht zeigen Situationen an, die Maschine beschädigen können.
PRECAUCIÓN
Las precauciones indican las situaciones que pueden dañar la máquina.
Risk of Electric Shock
Risque électrique de décharge
Gefahr des elektrischen Schlages
Riesgo eléctrica de la descarga
Protected Earth Ground

© 2007–2011 Rigaku Automation, Inc. vi 401718 Rev A
Part Names
Parts of the software appear in the manual with the first letter of the word capitalized and the
entire word in italics. Thus, “job” refers to something the operator or system would do, but “Job”
refer to a software component.
Terminology
This section presents descriptions and definitions of terms used in this document.
A chemical is a substance that can be purchased. A chemical probably has a CAS number
associated with it. The units of measurement suggest the phase or type of the chemical and the
information required, for example:
Units of M suggest an ionic solid (for example, ammonium sulfate)
Information required: formula weight, default stock, solubility, ionic makeup
Units of %w/v suggest a polymer (for example, PEG 3350)
Information required: default stock
Units of %v/v suggest a liquid (for example, Dioxane)
Information required: density
A Sample is a specific preparation of the protein that is set up in a crystallization experiment.
For example, a Sample could be 25mg/ml lysozyme in acetate, 50% BSA.
Astock is a liquid that is used in the lab:
NaCl is a chemical
5M NaCl is a stock
A default stock is generally the concentration of the aqueous solution of a chemical that is sold
by Hampton Research.
A reservoir design is a collection of chemicals that make up the reservoirs of a plate.
A standard screen is a crystallization screen that can be purchased from outside vendors.
A drop design is a collection of chemicals in the crystallization drop. A drop may consist of a
protein sample and a reservoir, or a protein sample and any collection of chemicals.
A sitting drop experiment is a vapor diffusion experiment with a reservoir design associated
with it.
A hanging drop experiment is a vapor diffusion experiment with a reservoir design associated
with it.
An oil experiment is a microbatch experiment with a sets of drop parameters and a volume of
oil associated with it.
A recipe is the set of instructions for preparing the chemicals specified by a design for
reservoirs and drops of a plate, including the quantities of stocks used in each.
A class is a classification for the chemicals used in the CrystalTrak database. Salt, polymer, and
buffer are classes.
A property is an associated descriptor which can be used to search or filter data. For example:
pH, autoscore=clear, acetate, and ammonium sulfate are properties.

© 2007–2012 Rigaku Automation, Inc. 1401718 Rev A
CHAPTER 1. INTRODUCTION
This manual provides operation and technical information for the Phoenix HT system and its
functions.
Also known as the Phoenix RE, the Phoenix HT (High Throughput) system is an enclosed station
in a CrystalMation system.
The Phoenix DT (Desktop) system is a standalone system.
1.1. General Description
The Phoenix HT system creates experimental plates using screens contained in deep-well blocks
and protein samples.
A dispense head containing 96 syringes draws specific volumes of chemicals from a deep-well
block and places them in a plate used for protein crystallization experiments (experimental
plate).
A single-channel nano-dispenser draws specific volumes of solution from sample vials and
dispenses them in experimental plates.
The Rigaku software interface acts as an executive for the Phoenix HT system; the Art Robbins
Instruments’Phoenix software can be accessed for maintenance purposes.
Figure 1. CrystalMation Phoenix HT

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Figure 2. Phoenix Base System
A Phoenix HT system is part of a CrystalMation system.
Functions included with a CrystalMation Phoenix HT system are:
An enclosure to contain the entire system.
An Emergency Motor Off (EMO) button to stop the motors immediately in an emergency.
Start and Stop buttons.
An active nest, to precisely and automatically position an experimental plate.
An antistatic curtain, to reduce static electricity that can interfere with drop placement.
A protein chill block, to chill vials of protein or other substances.
Dual chimney wash station.
1.2. Phoenix HT System Major Components
The Phoenix HT instrument consists of a base Art Robbins’ system and additional components.
1.2.1 Phoenix Base System
Follow all safety cautions and warnings in “1.4. Potential Hazards” on page 9. For cabling and
electrical connections, see “Chapter 2. Installation” on page 10.
Figure 3. Phoenix Base System
96-Syringe
Dispense
Head
PlateDeck
P h o e n i x B a s e S y s t e m
Nano-Dispenser

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1.2.2 Enclosure Status Light Indicators
The status lights for the Phoenix HT instrument indicate the following conditions.
1.2.3 Protein Chiller Block
The protein chiller block uses a thermo-electric temperature controller to maintain a user-specified
temperature to prevent denaturation of protein samples. There are 16 temperature-controlled slots
and 2 slots that are not temperature-controlled, or ambient temperature. Each slot holds a 200 µl
PCR tube. The temperature is set using the interface on the thermo-electric control unit (Figure 3).
Figure 4. Protein Chiller Block
Color Standard Definition Machine Specific
All Off System has no power
Solid Red Power is on, software launched, software in
stopped state, or machine is not ready to process
orders. Machine can not start processing orders.
Stopped, not ready for plate to be
made.
Blinking Red Power is on, software launched, system has an
error condition and system has stopped, or the
system requires initialization, or the EMO is active.
Not initialized, EMO pressed,
liquid handling error.
Solid Amber Power is on, software launched, no errors but
supplies are low and system will run out and stop
soon, or the temperature is out of specification.
Supplies low.
Blinking Amber Power is on, software launched, no errors but
system has run out of supplies and stopped, or the
software is paused, or the doors are open or
unlocked.
Software paused, door open, low
water pressure.
Solid Green Power is on, software launched, no errors,
software is in idle state, waiting on orders. The
machine will automatically process orders when
they become available.
Idle, waiting on orders.
Blinking Green Power is on, software launched, no errors, system
is running, actively processing/moving.
Order being processed.
Blinking
Amber+Red
Power is on, software is not launched or control
system is not responding.
Application software not
connected.
Ambient temperature slots Temperature-controlled slots
1 2 3 4 5 6 7 8 . . . . . . 16 Slot Numbers

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In the Phoenix HT enclosure, when accessing the the chiller block through the left-side access
window, the location numbers are in reverse order because the Phoenix system faces the
Integration Module.
Figure 5. Phoenix HT Chiller Block Slots
Figure 6. Temperature Controller Front Panel
Figure 7. Temperature Controller Rear Panel
To apply power to the Protein Chiller Block, flip the rocker switch on the back of the box labeled
Thermoelectric Temperature Controller underneath the deck.
To adjust the temperature, push and hold the SET button until the number on the display begins
flashing. Use the arrow keys to adjust the temperature setting. Push SET when the desired
setpoint is reached.
Always set to 7 V
Click to power ON.

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1.2.4 Wash Stations
The 96-head wash station and the nano wash station are supplied with wash water from the water
pump control box. The pump control box controls the flow of the wash water.
Water enters the controller from the water pump control box, and is pumped through one line
to the nano-wash station, and through two lines to the 96-head wash station.
The three lines are controlled by three valves.
Located on the right side of the water pump control box, push buttons prime the pumps, a
process that removes air in the lines and allows free water flow.
Waste water from the wash station chimneys flows from the wash station drain directly to a
waste-water tank.
Figure 8. Water Pump Control box (Front)
Located on the right side of the pump control box, push buttons on the electronics control box
prime the pumps, a process that removes air in the lines and allows free water flow.
Figure 9. Buttons to Prime the Pumps
Flow control valves
Water lines to wash stations
Water line from supply

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The 96-head wash station has 96 channels, one for each needle in the 96-dispenser head, to
increase wash efficiency and eliminate cross contamination. The wash station is mounted on
the deck in Slot 8.
Figure 10. Wash Station Chimneys
The nano wash station is a wash station for the single-tube head, to increase wash efficiency and
eliminate cross contamination.
1.2.5 Nano-Dispense Controller
Located inside the enclosure, the nano-dispense controller dispenses the precise nano-liter drops
of protein or other samples.
Figure 11. Nano-Dispense Controller, Front
96-head wash
station chimney
Nano wash station
chimney
Supply line to the nano dispenser tip
Switching valve between washing/aspirate and
dispensing functions
Syringe used to dispense/aspirate
Electrical connector which provides the black line tip
trigger and two grey lines used as level sensors for the
reservoir bottles
Each reservoir bottle has a black line to pressurize and
a clear reagent line to supply wash fluid from the
reservoir bottles
Clear wash supply line to the switching valve
Black line tip trigger that opens and closes the nano dispenser tip
Switch to unpressurize the reservoir bottles to fill
them, and then pressurize them

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Figure 12. Nano-Dispense Controller, Rear
1.2.6 Anti-Static Bar
The anti-static bar removes static electricity from crystallization plates before use. Static electricity
can cause the dispense drops to shift inside the subwells. Static charges are removed from plates
that pass in proximity to the anti-static bar.
1.2.7 Active Nest
An Active Nest positions a plate against the indexing pins under software control for accurate
dispensing. The shelf must be in the front position and contacting the electrical pins in order to
complete the software command to open the active nest. Flexinol® wires are activated to pull two
rollers in the upper right corner away from the center of the nest. When the rollers close, the plate
is pushed against the indexing pins. The active nest is normally in the closed position.
Figure 13. Active Nests
Nano trigger connection to Phoenix
base system rear panel
USB cable to computer
Cable to power supply adapter

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1.2.8 Plate and Shelf Sensors
A sensor bar over the shelves holds the plate sensors and the shelf sensors. The shelf sensors
determine whether the upper or lower shelf is in the sensing position. Plate sensors determine
whether a plate is present in a specific position on a shelf.
Figure 14. Sensor Bar for Plate and Shelf Position Sensors
1.2.9 Active Nest Terminals
The Active Nests, if installed, can receive commands only when the terminals on the shelf are in
contact with the terminals on the front of the base unit.
Figure 15. Active Nest Terminals
1.3. Safety Features
The basic safety features that have been designed into the system include:
Servo motors tuned for safety
Grounded power supply system
Enclosed work area (CrystalMation system only)
Access door interlocks (CrystalMation system only)
Shelf
position
sensors
Plate sensors
Terminals for active nests
(lower shelf terminals are connected)

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1.4. Potential Hazards
The following areas of operation may be associated with hazards to persons and equipment.
Contact the Rigaku Automation Service Department for assistance if you have questions about
safe operation of the instrument.
WARNING: Always disconnect the system from electrical power prior to servicing, cleaning,
or moving this equipment.
Networking
Using the Control Software
Electric power hazards
Moving the Phoenix HT system from cold to warm areas
Opening enclosure access doors
If a failure occurs in the Phoenix HT system, contact the Rigaku Automation Service Department
for failure repair.
1.4.1 Networking
The control computer and the Database Server are networked together. The Database Server can
be connected to an external network.
CAUTION: DO NOT connect the control computer directly to an external network. Make
any external network connection for the Phoenix system through the Database Server.
The control computer is connected via Ethernet to the Database Server.
1.4.2 Electric Power Hazards
The Phoenix HT system operates using 100-240 VAC. Keep any moisture or foreign objects out of
the enclosures. Do not apply power to the equipment in wet environments or when moisture may
have entered the equipment.
WARNING. Risk of Electrical Shock. DO NOT open the electrical enclosure. The
electrical enclosure is a factory service item ONLY.
1.4.3 Moving the Phoenix HT System from Cold to Warm Areas
Condensation may form on interior surfaces when the equipment is moved from a cold room to a
warm room. Ensure that the equipment has reached ambient temperature before operating.
1.5. Rigaku Service and Support
Our in-house service personnel and application scientists back our field service force; we are
ready to serve you.
Contact us through the help desk, email, or online requests.
Service help desk
General: 1-888-362-2324 (answered 24/7) [281-362-2300 x 132]
European-based single-crystal customers: +(44) 1732 765 008
By e-mail:
General: [email protected]

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© 2007–2012 Rigaku Automation, Inc. 10 401718 Rev A
European-based single-crystal customers: [email protected]
Online service request form: Located on http://www.rigaku.com/contact/service.htm
If the system fails, note what happened, any messages that appear on the monitor, lamp displays,
what the system was doing before the failure, and any steps that were taken to correct the
problem. Then contact the Rigaku Automation Service Department for assistance.

© 2007–2012 Rigaku Automation, Inc. 11 401718 Rev A
CHAPTER 2. BASICS
This chapter contains instructions for setting up, starting and shutting down the system. The
Phoenix HT system creates experimental plates using screens contained in deep-well blocks and
protein samples.
The 96-syringe dispense head is used to transfer an 8x12 array of solutions simultaneously.
For protein crystallography, it is used for transferring a crystallization screen from a deep well
block into the reservoirs of an experimental plate.
The nano-dispenser aspirates from a single source location and dispenses a small volume to
many locations. For protein crystallography, it is used to aspirate protein from a sample tube
and dispense drops into the experimental plate.
For basic setup, starting, and shutting down procedures as well as status light usage, see:
“Appendix B. Facilities Layout and Cabling” on page 40
“2.1. System Power-On” on page 11
“2.2. Shutting Down the System” on page 12
“2.3. Running the System” on page 12
“2.4. Status Light Indicators” on page 12
“2.5. Using the Stop, Start, and Emergency Stop (EMO) Pushbuttons” on page 13
“2.6. Resetting the System after an Emergency Motor Off (EMO) Shutdown” on page 13
2.1. System Power-On
The power-up sequence for the CrystalMation Phoenix HT instrument follows:
1. Rock the power switch on the outside of the enclosure to the | position.
2. Power on the control laptop computer and log into Windows.
3. Power on the Phoenix HT base system using switch on the back.
4. Power on the Nano-Dispense Controller.
5. If applicable, power on the temperature controller.
6. Start the Rigaku Phoenix HT software. (The Art Robbins’ Phoenix software is automatically
started.)
7. Twist the red Emergency Motor Off (EMO) button to reset the system.
8. Push the lit green Start button to reset power.
9. Click the green icon in the Phoenix HT software to home the motors in the instrument.
When the motors are homed, the instrument is ready for processing.

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2.2. Shutting Down the System
The power-down sequence for the CrystalMation Phoenix HT instrument is:
1. Click the Disconnect icon in the Phoenix software.
When asked if you wish to disconnect from the Phoenix RE software, click OK.
When asked if you wish to disconnect from the Art Robbins Phoenix software, click OK.
2. Push the red Emergency Motor Off (EMO) button.
3. Power off the Nano-Dispense Controller.
4. Power off the Phoenix HT instrument.
5. Shut down the laptop computer via Windows.
6. Rock the power switch on the outside of the enclosure to the 0 position.
2.3. Running the System
Follow the operating procedures current in your laboratory setting. Refer to the guidelines and
instructions in the original-equipment Phoenix Setup Guide, in “Chapter 3. Quick Start Procedures”
on page 14, and in the “Chapter 4. Phoenix HT Application Reference” on page 19 of this manual.
2.4. Status Light Indicators
The status lights for the Phoenix HT enclosure indicate the following conditions.
Color Standard Definition Machine Specific
All Off System has no power
Solid Red Power is on, software launched, software in
stopped state, or machine is not ready to process
orders. Machine can not start processing orders.
Stopped, not ready for plate to be
made.
Blinking Red Power is on, software launched, system has an
error condition and system has stopped, or the
system requires initialization, or the EMO is
active.
Not initialized, EMO pressed, liquid
handling error.
Solid Amber Power is on, software launched, no errors but
supplies are low and system will run out and stop
soon, or the temperature is out of specification.
Supplies low.
Blinking Amber Power is on, software launched, no errors but
system has run out of supplies and stopped, or
the software is paused, or the doors are open or
unlocked.
Software paused, door open, low
water pressure.
Solid Green Power is on, software launched, no errors,
software is in idle state, waiting on orders. The
machine will automatically process orders when
they become available.
Idle, waiting on orders.
Blinking Green Power is on, software launched, no errors, system
is running, actively processing/moving.
Order being processed.
Blinking
Amber+Red
Power is on, software is not launched or control
system is not responding.
Application software not
connected.

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2.5. Using the Stop, Start, and Emergency Stop (EMO) Pushbuttons
Located on the Phoenix HT enclosure, the Start, Stop, and EMO pushbuttons are used as follows:
CAUTION: If there is any concern at all about instrument crashing, use the EMO to
stop the machine.
2.6. Resetting the System after an Emergency Motor Off (EMO) Shutdown
To reset from an EMO state:
1. Rotate the red EMO button clockwise until it pops out.
2. Push the green push button to restore power. The Phoenix HT application restarts the Art
Robbins Phoenix application.
3. Push the green start button. The Phoenix HT application homes the motors. The Art Robbins
Phoenix application connects.
4. Remove and discard the block that was in process.
5. Perform a wash.
6. In the Jobs View, select the job that was running when the EMO button was pushed.
7. Select Jobs from the menu bar, and select Edit Job. Check the Reset job status check box to reset
the job.
8. To rerun the protocol, load and run the job.
EMO Immediately shuts off power to the Phoenix HT instrument, stops movement, and stops
the Art Robbins Phoenix application.
Use the EMO button when there is a danger to the machine or to persons, for example,
if a tall plate is in a short plate slot.
See also “2.6. Resetting the System after an Emergency Motor Off (EMO) Shutdown” on
page 13.
Stop Pauses the Phoenix HT system at the end of the current protocol step.
Performs a “soft” stop, running the instrument until the end of the current protocol step
before stopping.
Start Starts or resumes the sequence.
Restores power after an EMO, and also performs the same functions as the Start button
in the Rigaku Phoenix software.
Reset job status
check box

© 2007–2012 Rigaku Automation, Inc. 14 401718 Rev A
CHAPTER 3. QUICK START PROCEDURES
This chapter outlines basic steps used with the Phoenix HT system. The preliminary setup
includes the physical positioning and registration of protein samples and the creation of jobs in
Crystaltrak. The Rigaku Phoenix software procedures define the locations of the protein samples,
check for adequate liquid volumes, and enable the running of the Phoenix system .
3.1. Preliminary Setup
1. Open the left-hand access door of the Phoenix HT enclosure and manually insert the sample
tubes into the protein chiller block.
Note the slot location where you place each tube; for example, slot 16 is the first slot as you
reach into the enclosure.
IMPORTANT: Be sure that the cap to the tube is removed before inserting the tube into the
chill block. Failure to do this can result in damage to the nano dispenser needle.
2. If you are not using the Integration Module to automatically load plates, with the same left-
hand access door still open, position a crystallization plate and a deep well block containing
the desired screen into the Phoenix plate nests located on the top two shelves.
If not using the Integration Module, then the manually added plates/block must be registered
in the Manage Device/Onboard plates view.
Table 1. Plates Approved for the Phoenix HT System
Plate Type Plate Catalog Number Barcode Prefix
Single-well Greiner CrystalQuick Low Profile Greiner 609171 or 609871 S0
Multi-well Art Robbins’ Intelliplate 3-well Low Profile
(also known as CrystalMation plate)
ARI 102-0001-13 RZ
A
ccessing the chiller block
f
rom the left-hand access
d
oor