serva BlueVertical PRIME User manual
2
WARNING
These units are capable of delivering potentially lethal voltage when
connected to a power supply and are to be operated only by qualified
technically trained personal.
Please read the entire operator’s manual thoroughly before operating this
unit.
The BlueLine vertical electrophoresis systems are designed to give long
service and reproducible results in your laboratory. A few moments spent
reading these instructions will ensure that your expectations are reflected in
the successful use of the apparatus.
First check that the apparatus has been received complete and undamaged following
shipment. Any faults or losses must be notified to responsible SERVA Electrophoresis
GmbH distributor immediately. SERVA Electrophoresis GmbH Heidelberg cannot
accept responsibility for goods returned without prior notification.
Refer to the packing list and check that all components and accessories are present.
Warranty is 12 months from the date of delivery.
Please retain all packaging materials until the warranty period has expired.
Ver. 02/13
DOMINIQUE DUTSCHER SAS
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Contents
1. Packing List 4
2. Specifications 4
3. Operational conditions 5
4. Using the vertical gel electrophoresis unit 5
4.1. Safety precautions 5
4.2. General care and maintenance 6
4.3. Gel and buffer volumes/running conditions 6
4.4. Performing electrophoresis 7
4.4.1. Insertion of pre-cast gel cassettes into the inner core running unit 7
4.4.2. Sample loading 8
4.4.3. Start of electrophoresis 8
4.4.4. End of run 8
5. Recommended reagents for vertical electrophoresis 10
DOMINIQUE DUTSCHER SAS
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1. PACKING LIST
BlueVertical™ PRiME™
Cat. no.: BV 104
No. of
Items Description Cat. no.
1 Main Unit BV 104
1 Dummy Plate BV 104-7
2. Specifications
• Rugged acrylic construction
• All acrylic joints chemically bonded
• Doubly insulated cables, rated safe up to 1000 volt
• Gold plated electrical connectors, corrosion-free and rated safe up to 1000 volt
• Safety lid with integrated power connectors
• Pure platinum electrodes (0.2 mm diameter)
• Size: 16 cm x 15.6 cm x 9.5 cm (w x h x d)
• Weight: 1.2 kg
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Table 1: Specifications
Volume inner buffer chamber 200 ml
V
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r
b
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e
r
c
h
a
m
b
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r
4
5
0
m
l
Voltage (max) 500 V
Current (max) 250 mA
Operating temperature 4 °C – 65 °C
Electrodes Platinum wire (0.2 mm, 99.99 % pure)
Dimensions 16 x 15.6 x 9.5 cm (WxHxD)
Weight 1.2 kg
3. Operational conditions
This apparatus is intended for indoor use only. Maximum relative humidity up to
80 % (for temperatures up to 31 °C) decreasing linearly to 50 % relative humidity
(for temperatures up to 40 °C), at maximum altitude of 2000 m (MSL).
4. Using the vertical gel electrophoresis unit
4.1. Safety precautions
• Read the instructions carefully before using the apparatus.
• Always disconnect the electrophoresis unit from the power supply before removing
the safety cover.
• Do not attach the safety lid to the internal gel running unit while it is out of the
bottom buffer chamber.
• Do not exceed the maximum operating voltage or current (see Table 1).
• Do not operate the electrophoresis units in metal trays.
• Polymerised gels may contain some unpolymerised acrylamide monomer, which is
a volatile, cumulative neurotoxin and suspected carcinogen. Wear effective
protective clothing and gloves. Follow recommended handling and disposal
procedures.
• Do not fill the unit with running buffer above the maximum fill lines.
• Do not move the unit when it is running.
• Caution: During electrophoresis very low quantities of various gases are produced
at the electrodes. The type of gas produced depends on the composition of the
buffer employed. To disperse these gases make sure that the apparatus is run in
a well ventilated area.
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4.2. General care and maintenance
• Before use clean and dry the apparatus with distilled water only.
Important: Acrylic plastic is not resistant to aromatic or halogenated
hydrocarbons, ketones, esters, alcohols (over 30 %) and acids (over 25 %).
• Before use, and then on a monthly basis, check the unit for any leaks at the
bonded joints. Place the unit on a sheet of dry tissue and then fill with distilled
water only to the maximum fill line. Any leakage will cause dampening of the
tissue paper. If any leakage is seen do not attempt to repair or use the
apparatus, but notify SERVA Electrophoresis GmbH Heidelberg resp. the
SERVA Electrophoresis GmbH distributor immediately.
• The platinum electrodes are partially shrouded for protection. However, when
cleaning the main tank do not use cleaning brushes in the electrode area. Usually
a thorough rinse with distilled water is all that is required.
• Ensure that the connectors are clean and dry before usage or storage.
4.3. Buffer volume/running conditions
The buffer volume can be seen in Table1. The running conditions (Table 2) vary
according to the number of gels and their composition. The current required will increase
in proportion to the number of gels providing that the voltage is not limiting, e. g. two gels
require twice the current of one but the same voltage.
By increasing the gel concentration the electrical resistance is increased and the rate of
migration decreases. Higher voltages can be applied but be careful not to overheat the
gel.
Table 2: Running conditions using BlueVerticalTM in different electrophoresis applications
Type of electrophoresis Type of SERVAGel
T
M
Precast Gel Running conditions
SDS PAGE SERVAGelTM HSE • Voltconst.: 400 Volt
• Time: 20 minutes
SDS PAGE SERVAGelTM TG PRiMETM • Voltconst.: 300 Volt
• Time: 35 minutes
SDS PAGE SERVAGelTM TG Neutral • Voltconst.: 150 Volt
• Time: 70 minutes
Native PAGE SERVAGelTM N • Voltconst.: 130 Volt
• Time: 60 – 120 minutes
Isoelectric Focusing SERVAGelTM IEF
• Voltconst.: 100 Volt for 60 minutes
• Voltconst.: 200 Volt for 60 minutes
• Voltconst.: 500 Volt for 30 minutes
•
T
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e
:
1
5
0
m
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s
For detailed information about the running conditions of SERVAGel™precast vertical mini
gels, please see the manuals supplied with the gels.
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4.4. Performing electrophoresis
4.4.1. Insertion of precast gel cassettes into the inner core running unit
•Clean the silicone gasket, located in the inner core unit. If the gasket becomes
unseated from its groove simply press it back into place.
•Insert the precast gel cassette into the inner core running unit.
•When running only one gel, a dummy plate is required on the opposite side to
retain the required buffer level.
•Place the gel running unit into the buffer tank using the locating pegs as guides.
•Add the appropriate volumes of running buffer to the inner and outer buffer
chambers (see Table 1).
•Important: Do not overfill (please see marking “max fill”).
1 2 3
4 5 6
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4.4.2. Sample loading
•Load the samples using a gel loading pipette tip. During sample loading the
pipette tip should be 1 - 2 mm above the bottom of the well to minimise dilution of
the sample and to keep the sample as a tight layer.
•Avoid cross-contamination by overloading the sample wells.
•Fill unused wells with the equivalent volume of sample buffer to maintain uniform
electrical resistance across the gel.
4.4.3. Start of electrophoresis
•Close the safety lid firmly making sure that the electrical connectors form a good
contact.
•Connect the electrophoresis apparatus to the power pack and connect the power
pack to the mains supply, e. g. SERVA BluePower 500x4 (BP-500x4). Turn all
settings to zero before turning on the mains supply. Adjust the controls to the
desired settings. Follow manufacturers instructions.
4.4.4. End of run
•Turn the power supply settings to zero, turn off mains supply and disconnect the
power leads.
•Remove the safety lid, take out the internal gel running unit and discard the buffer.
1 2 3
4 5 6
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•Discard the buffer from the outer buffer tank. Rinse the chambers twice with
distilled water then dry the electrode connectors with tissue. Ensure that the
connectors are clean and dry before usage or storage (see chapter 4.2).
For further information about the SERVA BlueLine, please contact the technical
service of SERVA Electrophoresis GmbH in Heidelberg, phone Tel.: +49 (0)6221
13840-44.
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5. Recommended reagents for vertical electrophoresis
SERVA reagents for electrophoresis underly stringent quality and application control to
ensure best performance and results. We recommend the usage of SERVA electro-
phoresis reagents especially along with BlueLine electrophoresis instruments as the
quality of consumables is fine-tuned to the equipment (application tests).
Precast gels and Starter Kits
SERVAGel™ Gels for SDS PAGE
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SERVAGel™ TG PRiME™ Starter Kit 43206
SERVAGel™ TG PRiME™ 8 % Tris-Glycine Gel 8 %
1
2
4
3
2
6
0
10 43261
SERVAGel™ TG PRiME™ 10 % Tris-Glycine Gel 10 % 12 43263
10 43264
SERVAGel™ TG PRiME™ 12 % Tris-Glycine Gel 12 % 12 43266
10 43267
2
D
4
3
2
6
8
SERVAGel™ TG PRiME™ 14 % Tris-Glycine Gel 14 % 12 43269
1
0
4
3
2
7
0
2D 43271
SERVAGel™ TG PRiME™ 4-12 % Tris-Glycine Gel 4-12 % 12 43273
10 43274
SERVAGel™ TG PRiME™ 4-20 % % Tris-Glycine Gel 4-20 % 12 43276
10 43277
SERVAGel™ TG PRiME™ 8-16 % % Tris-Glycine Gel 8-16 % 12 43279
1
0
4
3
2
8
0
2D 43281
SERVAGel™ TG 8 % Tris-Glycine Gel 8 %
(Available upon request, please inquire.) 12 43208
10 43209
SERVAGel™ TG 10 % Tris-Glycine Gel 10 %
(Available upon request, please inquire.) 12 43210
10 43211
SERVAGel™ TG 12 % Tris-Glycine Gel 12 %
(Available upon request, please inquire.)
12 43212
10 43213
2D 43226
SERVAGel™ TG 14 % Tris-Glycine Gel 14 %
(Available upon request, please inquire.)
1
2
4
3
2
1
4
1
0
4
3
2
1
5
2D 43227
SERVAGel™ TG 16 % Tris-Glycine Gel 16 %
(Available upon request, please inquire.) 12 43216
10 43217
SERVAGel™ TG 4-12 % Tris-Glycine Gel 4-12 %
(Available upon request, please inquire.) 12 43232
10 43238
SERVAGel™ TG 4-20 % % Tris-Glycine Gel 4-20 %
(Available upon request, please inquire.) 12 43230
10 43236
SERVAGel™ TG 8-16 % % Tris-Glycine Gel 8-16 %
(Available upon request, please inquire.)
1
2
4
3
2
3
1
10 43237
2
D
4
3
2
2
8
SERVAGel™ Neutral pH 7.4 12 43220
10 43222
SERVAGel™ Neutral pH 7.4 Gradient 12 43221
10 43223
SERVAGel™ Neutral HSE Starter Kit 12 43207
SERVAGel™ Neutral HSE
1
2
4
3
2
4
5
10 43246
2D 43247
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SERVAGel™ Gels for native PAGE (Blue Native BN/Clear Native CN)
Product Sample wells Cat. No.
SERVAGel™ N Native Starter Kit 12/10 43204
SERVAGel™ N 3-12, Vertical Native Gel 3-12 % 12 43250
10 43251
SERVAGel™ N 4-16, Vertical Native Gel 4-16 % 12 43252
10 43253
SERVAGel™ Gels for vertical IEF
SERVAGel™ IEF Starter Kit 12 43205
SERVAGel™ IEF 3-10, Vetical IEF Gel pH 3-10
1
2
4
3
2
4
0
10 43242
Buffers for SDS PAGE
Product Cat. No.
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SERVA Tris-Glycine/SDS Electrophoresis Buffer (10x) 42529
Laemmli Sample Buffer (2x), for SDS PAGE 42526
SERVA Tris-Tricine/SDS Sample Buffer (2x) 42551
SERVA Tris-Tricine/SDS Electrophoresis Buffer (10x) 42552
SERVA Tris-Tricine/SDS Electrophoresis Buffer (20x) 42560
Buffers for native PAGE
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Native Anode Buffer for BN/CN (10x) 42535
Native Cathode Buffer for BN/CN (10x) 42536
Sample Buffer for BN (2x) 42533
Sample Buffer for CN (2x) 42534
SERVA Blue G solution for BN , 1 % 42538
Buffers for vertical IEF
IEF sample buffer (2x), sterile filtered 42537
SERVAGel™ IEF Running Buffer Kit 42539
Reagents
Glycine 23390
Tris(hydroxymethyl)aminomethane (TRIS) 37190
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6
2
5
Dithiothreitol (DTT) 20710
Dithioerythritol (DTE) 20697
SERVA Blue G 35050
SERVA Blue R 35051
Bromophenol Blue-Na-salt 15375
SDS Solution, 20 % 20767
A comprehensive range of SERVA products for electrophoresis is available listed in the
Main Catalogue available from SERVA Electrophoresis. Please inquire.
DOMINIQUE DUTSCHER SAS
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