SPEX Genoseal 2060 User manual

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2060 Genoseal™
Semi-automatic Heat Sealer for Microplates and Tube Racks

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Contents
Before You Begin ...........................................................................................................................................3
Safety Considerations...................................................................................................................................3
Warning Symbols Used in Accordance With IEC 417...........................................................................................3
Introduction .....................................................................................................................................................5
Unpacking/Packing the Genoseal ............................................................................................................................5
Principle of Operation...................................................................................................................................5
Installation .......................................................................................................................................................6
Intended Use..................................................................................................................................................................6
Inventory ..........................................................................................................................................................6
Operating Environment ................................................................................................................................6
Connecting the Voltage Supply.................................................................................................................................6
Operation Procedure ....................................................................................................................................7
Optimization....................................................................................................................................................8
Temperature and Time Setting ..................................................................................................................................8
Sealing PCR Plates - No Skirt, Half Skirt..................................................................................................................8
User Interface .................................................................................................................................................9
Specifications of Genoseal ......................................................................................................................... 11
Troubleshooting ............................................................................................................................................ 11
Service and Repairs..................................................................................................................................... 12
Regulatory Information ............................................................................................................................... 12
CE Declaration of Conformity .................................................................................................................................. 12

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Before You Begin
Read this instruction manual. Failure to follow the instructions, in this manual, may result in damage to the unit,
injury to operating personnel, and poor equipment performance.
Caution: All internal adjustments and maintenance must be performed by qualified service personnel.
Material in this manual is for informational purposes only. The contents and the product it describes are subject
to change without notice. Spex makes no representations or warranties with respect to this manual. In no event
shall Spex be held liable for any damages, direct or incidental, arising from or related to the use of this manual.
©2022 Spex, an Antylia Scientific company. All rights reserved.
Safety Considerations
It is Important that the Genoseal unit is installed and operated in such a way that all applicable health and safety
requirements are met. It is the user’s responsibility to ensure that all relevant health and safety regulations are
identified and complied with. Failure to do so may result in damage to the equipment and may result in personal
injury. In particular, the user should study the contents of this guide carefully before handling or operating this
equipment.
Under no circumstances will the supplier of this equipment be liable for any incidental, consequential or any
special damages of any kind whatsoever, including but not limited to lost profits arising from, or in any way
connected with the use of this equipment or this instruction manual.
Warning Symbols Used in Accordance With IEC 417
This symbol used alone indicates important operating instructions which reduce the risk of injury or
poor performance of the unit.
Caution: This symbol, in the context of a caution, indicates a potentially hazardous situation which,
if not avoided, could result in minor to moderate injury or damage to the equipment.
Warning: This symbol indicates potentially hazardous situations which, if not avoided, could result
in serious injury or death.
Warning: This symbol indicates situations where dangerous voltages exist and potential for
electrical shock is present.
Caution: Protective Conductor Terminal

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Caution: Hot surface/hot area. This instrument is fitted with an internal heater with a maximum
temperature of 200 °C when in use.
This symbol indicates a need to use gloves during the indicated procedures. If performing
decontamination procedures, use chemically resistant gloves. Use insulated gloves for handling
samples and when using liquid nitrogen.
Caution: Keep hands clear during priming process to avoid risk of finger entanglement and
entrapment.
Before installing, using, or maintaining this product, please be sure to read this manual and product
warning labels carefully. Failure to follow these instructions may cause this product to malfunction,
which may result in injury or damage.
Main Switch Symbols
I = ON
0 = OFF
Below are important safety precautions that apply to this product:
Warning: Do not operate this instrument in an atmosphere containing explosive gases.
Warning: Only approved supplied power cord set must be used with this instrument. If it is required
to use an extension cord, the cord must be earthed. The Genoseal is supplied for direct connection
to normal 115 / 230 VAC, 50 / 60Hz supply, with a variation in supply that gives a voltage range of
108 VAC to 250 VAC. If you should require to clean the heater block, turn the power o and allow
the block to cool to ambient room temperature. You can now access the heater block and clean it.
The Genoseal is designed to be operated with a maximum sealing temperature of 200 °C.
The Genoseal should be switched on 5 minutes prior to use when plugged into 230 V and 6
minutes when plugged into 110 V. This is to enable the set temperature to be reached and to
stabilize. The Genoseal should only be used in a suitably ventilated area. The use of solvents on
the unit is not recommended. Certain components become hot during the correct operation of the
equipment. These components are marked with the hot surface symbol. Due care should be taken
to avoid personal injury. Spex accepts no responsibility for the misuse of this equipment.
Please ensure that heater block is at ambient temperature before cleaning and a senior operator carries out
the cleaning.

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Introduction
The Genoseal heat sealer has been designed to seal most types of microplates. Our product utilizes various
heat seal films and foils to give either permanent or peelable seals for all common microplate material types
(polypropylene, polystyrene, polyethylene). Seal times and seal temperatures are fully adjustable on the product
via the control screen. The Genoseal has dual voltage capability, operating on both 110 and 230 VAC supplies
without the need for a power transformer.
Unpacking/Packing the Genoseal
When unpacking your Genoseal, visually inspect the unit for any damage which may have occurred during
shipping. Do not use the unit if there is any damage. Check that all components are present. Refer to the
packing list.
The unit is supplied in a re-usable crate and a transit bracket (if applicable) that must be retained in case the
unit requires servicing. The Genoseal is a precision instrument and requires the proper packaging for shipping.
Inadequate packaging can lead to damage. Ensure that all packing foam and ties are removed prior to powering
on the unit.
Use appropriate PPE and necessary tools available in the facility to remove the unit from the crate properly.
Pack in the same way the unit was originally shipped, with correct foam and crate. If any damage was caused or
the original crate that the Genoseal came in is lost, please contact support.
Principle of Operation
The unsealed plate is placed on the shuttle which extends from the front
of the sealer, allowing full access for the user to the system. The drawer
unit is manually pushed into the unit. The plate sealing operation will vary
based on user settings and set sealing time. (See user inface section for
operation.) The drawer and the plate returns to its original start position
once the sealing process is complete.
The sealer operates as a stand-alone, manual unit. Each sheet of sealing
film is sucient for sealing one plate to cover the entire plate surface with
a fixed length of 125 mm, allowing peelable or automation compatible
applications. See Figure 1.
The sealing films are either aluminum or clear laminates and are able to produce permanent, pierceable or
peelable seals with polypropylene, polystyrene, polythene or COC plates.
To keep the size and weight to a minimum, the unit is fully electronic and all parameters, such as temperature
and time, are fully adjustable to ensure a perfect, even seal with dierent plates and film formats.
User safety has been carefully considered during the design process to eliminate risk of contact with hot
surfaces or the internal mechanism during operation.
Figure 1. 384-Well Sealed Plate

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Installation
The unit should be installed on a sturdy bench to avoid any vibration during operation. When locating the unit,
there should be enough space allowed for the drawer to move out without obstruction.
Intended Use
The Genoseal is intended to be used for the heat sealing of microplates or tubes during experimentation,
storage, or transportation. This device is not intended to be used as a medical device.
Caution: Use appropriate lifting and carrying equipment available in the facility during unit movement
from one place to another. Always use any necessary PPE during unit movement.
Inventory
Note: All of the items listed below are available as spare components from Spex.
DESCRIPTION PART NUMBER
Plate Adapter for PCR 384-Well Format 2060-PA-384
Skirtless Plate Adapter 2060-PA-96
Heat Sealing Film 2061
Plate Weight for Recessed Plates* 2060-WT-RC
Plate Weight for Standard and Rimless Plates* 2060-WT-STD
Plate Locators* 2060-PL
* Accessories supplied with the Genoseal
Operating Environment
The Genoseal should be operated in an environment with a temperature range of 10 °C to 30 °C and a non-
condensing relative humidity range of 10% to 80%.
The unit should be kept out of drafts and air currents, as they will have an adverse eect upon the temperature
stability of the sealing plate area. If the sealing plate area is subjected to drafts or air currents, quality or process
time may be aected.
The unit should be placed on a secure and stable surface with a minimum of 100 mm (4-inch) clearance on all
sides.
Connecting the Power Supply
The Genoseal utilizes a standard IEC inlet, which is fused and switched. Plug the IEC Lead in the inlet and switch
the Rocker I.O. Switch to the On position ( | ). The display screen should illuminate.
Note: The system is self-regulated to sense voltage dierences between 100 / 250 VAC.

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Operation Procedure
Plate Locators and Adapters
Plate locators are used specifically on skirted plates, it
allows for the plate skirt to sit around the corresponding
locator and support the underneath of the plate. To
ensure the correct plate locator is used, check the
dierence in the bottom of the well distance to the
plate skirt base, if there is a dierence, the center of
the plate can flex when pressed. This means a suitable
plate locator needs to be used to take up the dierence.
Poorly supported wells will result in an uneven seal
across the plate surface.
However, if you are using an unskirted plate where the edge of the plate skirt is the same level as the bottom
surface of the plate, you will have to use a plate adapter. They support each cell individually and ensure that the
seal is even. They are designed to support most plate types.
How to Begin the Sealing Process
The Genoseal seals microplates by the following method:
1. First set the temperature and sealing time of the unit.
2. Next, select the correct plate locator for the plate you want to seal.
3. Place the unsealed microplate on the top shuttle plate.
4. Place the pre-cut length of film/foil on the microplate and place the foil
brass weight on top of the foil on the microplate. Ensure that the foil is
centrally placed on the microplate.
Note: Some sealing material may curl when heated and the foil brass weight
will be required to keep the seal flat before sealing and to prevent the plate
from sticking to the heater block. Two types of foil weights are supplied with
each Genoseal; one for normal plates ( Figure 2 ), and one for plates with high
rims and recessed wells ( Figure 3 ).
5. The sealing process is started by pressing “seal” on the software. Once
pressed, begin by placing the correct plate locator, plate and plate weight
as demonstrated on the display. Thirdly, start pushing the drawer fully
into the unit, following the instructions on the display. Once the drawer is
pushed in it will automatically latch and the screen will display a sealing
message. You may now release your grip on the drawer handle. The
sealing process will finish itself based on the parameters entered.
6. When the seal has finished, the drawer will automatically open, presenting the plate for removal.
Note: Care MUST be taken when removing the foil loading tool, IT WILL BE HOT. Always hold it by the
specified tab as shown in Figures 2 and 3.
Plate Weight Tab
Figure 2. Normal plate weight
Figure 3. Recessed Plate Weight
Plate Weight Tab
Shuttle Plate Locator Plate

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Optimization
The Genoseal has been designed to reliably seal plates of dierent heights and dierent plastics, using a variety
of films, to give a seal with varying properties. These components will require dierent sealing conditions.
The quality and strength of the seal created between the sealing film will vary with dierent conditions. In
general, increasing either the sealing temperature or duration of seal, gives a stronger, more complete seal.
However, over-sealing on a regular basis is not recommended, as applying more heat can cause excessive
damage to the plate being sealed. This, in turn, would reduce the number of times a particular plate can be
resealed. Therefore, a balance has to be achieved, giving an acceptable seal with the minimal plate damage or
distortion.
Another optimization factor to be taken into account is the surface area of the plate. A plate with thin raised rims
around each well will have a reduced surface area, compared to a plate with wide raised rims. This would mean
that less heat is needed to seal the thin rim plate in comparison to the wide rim.
The pressure that the heater plate exerts during sealing is pre-set and cannot be adjusted. Plates that do not
have any raised rims are not usually suitable for heat-sealing.
Temperature and Time Setting
The following table gives a guideline as to the sealing temperature and time for dierent materials.
PLATE MATERIAL TEMPERATURE SETTING (ºC) TIME SETTING (SECONDS)
Polystyrene 145 to 165 2 to 5
Polypropylene 150 to 175 2 to 5
Polyethylene 150 to 170 2 to 5
Sealing PCR Plates - No Skirt, Half Skirt
An adapter will be required to seal plates which have well bottoms protruding past the plate skirt as well as
plates with no skirt. This is available from Spex.

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User Interface
Plug in the system to turn the power on. Once the system is turned
on, the Spex logo is displayed. The home screen appears as shown
to the left. If the system is in ECO mode, it will prompt you to wake it.
This screen will show upon power up. It will instruct you to close the
drawer to home the system and enable the unit to be ready to seal,
simply push the drawer in.
The screen will then show the sealing time, heater set temperature
and heater actual temperature along with the active status of the
heater block, cooling or heating depending on the temperature.
This is the main sealing screen. Here, you will see the main button to
press “seal” and begin the sealing process. Once you press this, you
will be prompted on how to complete the sealing procedure. Firstly,
place the correct plate adapter onto the shuttle, followed by the
plate and plate weight.
This screen will show once you press the seal button, it will prompt
you to ensure that the sealing plate is ready to be sealed, including
the correct plate support, the corresponding plate, the correct foil,
and finally the corresponding plate weight. Once all of these have
been checked, you can manually push the drawer in to begin the
sealing process. Once this is complete, the automated spring will
return the drawer back out.
Close drawer to
home system
Place plate foil
and foil weight
onto shuttle and
close drawer

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This screen will allow you to select the settings that
control the sealing time, temperature, ECO time and
scan a copy of the user manual through the QR code
displayed. After clicking settings, you will be shown this
screen, simply click the o
To adjust the set temperature of the heater block and
control the temperature that the Genoseal seals at, click
the first option showing a thermometer. Here you can
adjust the temperature up or down with the arrows on
the right side of the display. Once satisfied with the set
temperature, press “save”.
You can also adjust the sealing time by pressing the
stopwatch icon on the settings screen. Press it and adjust
it using the arrows on the right side of the display. Once
satisfied with the set sealing time, press “save”. If you
want to keep the time unchanged, press “cancel”.
To access the eco-mode timer, press the icon that shows
the flower in a light bulb icon from the settings screen.
This will enable you to set a time limitfor how long the
unit is not in use before automatically turning o the
heaters and going into eco-mode. Use this screen similar
to how you set the temperature and time.
This will show you the QR code which is scannable with
any QR code scannable smart phones or QR scanners
for a desktop view. It will load a PDF in your default PDF
viewer.
If the unit encounters an error at any stage, it will display an error
screen prompting you to power cycle the machine or reset it. (Error
screens may vary.)

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Specifications of 2060 Genoseal
Power Supply 115 / 230 VAC, 50 / 60 Hz
Power Consumption 600 watts nominal
Fuse Rating 5 amp anti-surge (T5AH 250 V)
Internal Heaters 4 x 100 watts (sealing plate)
Minimum Plate Height 9 mm
Maximum Plate Height 54 mm
Maximum Sealing Temperature 200 °C
Protection IP20
Cycle Time (approx.) 10 to 12 seconds (based on a 2 second seal)
Dimensions (depth x width x height) 12.13 in x 8.1 in x 15.3 in (308 mm x 205 mm x 389 mm)
Unit Weight 19.8 lb (9 kg)
Troubleshooting
If the problem with the Genoseal unit persists after the below actions have been carried out, contact Spex for
further investigation.
PROBLEM ACTION
Genoseal will not power up after
switching on Check fuse
Foil will not seal properly on a
microplate
1. Check seal temperature and time and reset if necessary
2. Ensure the correct height of plate locators are being used
“Error up switch” is displayed on the
screen after a seal is run
Ensure the correct plate locator/combination is being used and restart
the machine
Poor plate seal in middle of plate Check if plate supports are required. If required, contact Spex
The unit fails to respond to
commands Restart the machine
If the problem with the Genoseal persists after the above actions have been carried out, contact Spex for futher
investigation.

spexsampleprep.com
Phone: [phone]
[e-mail]
4819M
Connect with us
Operating Manual
Service and Repairs
For all requirements for service and repairs please contact: Spex at +1.732.549.7144.
Note: For any spare components, please refer to the inventory list and contact Spex for pricing information.
Regulatory Information
CE Declaration of Conformity
As detailed under the European Machinery Directive 89/392/EEC (amended by 91/368/EEC) and under the
UK legislation. The supply of Machinery (Safety) Regulations 1992 (SI 1992/3073). As detailed under The
Electromagnetic Compatibility Directive 89/336/EEC (amended by 91/263/EEC and 92/31/EEC and the UK
legislation, the Electromagnetic Compatibility Regulations 1992. As detailed under The European Low Voltage
Directive 73/23/EEC (amended by 93/68/EEC) and the UK legislation, The Electrical Equipment (Safety)
Regulations 1994.
The Declaration of Conformity is provided for the following equipment:
2060 Genoseal
Serial Number: To be included
Transposed Harmonized Standards
BSEN 12100-1 Safety machines: concepts
General: principles for design; basic terminology and methodology
BSEN 12100-2 Safety of machines: basic concepts, general principles for design; technical principles BSEN 61010
Safety requirements for electrical Equipment for measurement, control and laboratory use BSEN 60204 safety of
Machinery; electrical equipment of machines (section 19).
BSEN 50081-2 Electromagnetic Compatibility; generic emission standard, industrial environment.
BSEN 61000-6-2 Electromagnetic compatibility (EMC) Generic standard, immunity for industrial environments.
Other Standards: BS5378 safety signs and colors.