Telstar LyoQuest Troubleshooting guide

USER & SERVICE MANUAL
MANUAL DE USUARIO Y MANTENIMIENTO
Laboratory Freeze Dryers
Liofilizadores de laboratorio

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INDEX / ÍNDICE
1. GENERAL DATA ..................................................................................................................................... 6
2. DETAILS OF THE FREEZE-DRYER ............................................................................................................. 7
2.1. Aim of this manual ................................................................................................................................................... 7
2.2. Application ............................................................................................................................................................... 7
2.3. Requirements ........................................................................................................................................................... 7
2.4. Description ............................................................................................................................................................... 7
3. TECHNICAL CHARACTERISTICS ............................................................................................................... 9
4. TRANSPORT AND PACKING ................................................................................................................. 10
5. BEFORE START- P ............................................................................................................................... 10
5.1. Placing on site ........................................................................................................................................................ 10
5.2. Electrical connection .............................................................................................................................................. 10
5.3. acuum pump ........................................................................................................................................................ 10
5.4. Refrigerating unit ................................................................................................................................................... 11
6. RECOMMENDATIONS .......................................................................................................................... 11
6.1. Ice condenser ......................................................................................................................................................... 11
6.2. Greasing ................................................................................................................................................................. 11
6.3. Long stoppage ........................................................................................................................................................ 11
7. WORKING PRINCIPLE .......................................................................................................................... 12
7.1. Freeze ..................................................................................................................................................................... 12
7.2. Lyophilization ......................................................................................................................................................... 12
7.2.1. In manifold .......................................................................................................................................................... 12
7.2.2. In chamber .......................................................................................................................................................... 13
7.2.3. End of the process ............................................................................................................................................... 14
7.3. Lyophilization modes ............................................................................................................................................. 14
7.4. Selectable processes .............................................................................................................................................. 14
8. OPTIONAL ACCESORIES (COMPILATION AT THE END OF THE MAN AL) ................................................ 15
8.1. Chambers ............................................................................................................................................................... 15
8.2. Manifolds ............................................................................................................................................................... 16
8.3. Flasks condensations collector (Drip tray) ............................................................................................................. 16
8.4. Lyoquestlogger software ....................................................................................................................................... 17
9. INSTR CTIONS FOR SE ...................................................................................................................... 18
9.1. Start up ................................................................................................................................................................... 18
9.2. Semiautomatic mode ............................................................................................................................................. 19
9.3. Automatic mode .................................................................................................................................................... 19
9.4. Defrosting mode .................................................................................................................................................... 20
9.5. Menu ...................................................................................................................................................................... 20
9.6. Hours counter vacuum pump ................................................................................................................................ 21
9.7. Stop of the freeze-dryer ......................................................................................................................................... 21
9.8. No Plus version ...................................................................................................................................................... 22
10. RECIPES .............................................................................................................................................. 23
11. ALARMS .............................................................................................................................................. 23
12. MAINTENANCE PLAN .......................................................................................................................... 25
12.1. acuum unit maintenance ..................................................................................................................................... 25
12.2. Refrigerating system maintenance ........................................................................................................................ 27
12.3. Surface cleaning and disinfection .......................................................................................................................... 27
12.4. Maintenance table ................................................................................................................................................. 27
13. TRO BLESHOOTING ............................................................................................................................ 28
13.1. acuum unit ........................................................................................................................................................... 28
13.2. Refrigerating unit ................................................................................................................................................... 28
13.3. Freeze-dryer kit ...................................................................................................................................................... 29

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1. DATOS GENERALES ............................................................................................................................. 31
2. DATOS DEL LIOFILIZADOR .................................................................................................................... 32
2.1. Objetivo del manual ............................................................................................................................................... 32
2.2. Aplicación ............................................................................................................................................................... 32
2.3. Requerimientos ...................................................................................................................................................... 32
2.4. Descripción ............................................................................................................................................................. 32
3. CARACTERÍSTICAS TÉCNICAS ............................................................................................................... 34
4. TRANSPORTE Y EMBALAJE .................................................................................................................. 35
5. ANTES DE LA P ESTA EN MARCHA ...................................................................................................... 35
5.1. Emplazamiento del equipo .................................................................................................................................... 35
5.2. Conexión eléctrica .................................................................................................................................................. 35
5.3. Bomba de vacío ...................................................................................................................................................... 35
5.4. Grupo frigorífico ..................................................................................................................................................... 36
6. RECOMENDACIONES ........................................................................................................................... 36
6.1. Condensador de hielo ............................................................................................................................................ 36
6.2. Engrase ................................................................................................................................................................... 36
6.3. Paro prolongado .................................................................................................................................................... 36
7. PRINCIPIO DE F NCIONAMIENTO ........................................................................................................ 37
7.1. Congelación ............................................................................................................................................................ 37
7.2. Liofilización............................................................................................................................................................. 37
7.2.1. En manifold ......................................................................................................................................................... 37
7.2.2. En cámara ........................................................................................................................................................... 38
7.2.3. Finalización del proceso ...................................................................................................................................... 39
7.3. Modos de liofilización ............................................................................................................................................ 39
7.4. Procesos seleccionables ......................................................................................................................................... 39
8. ACCESORIOS OPCIONALES (RECOPILATORIO AL FINAL DEL MAN AL) ................................................... 40
8.1. Cámaras ................................................................................................................................................................. 40
8.2. Manifolds ............................................................................................................................................................... 41
8.3. Recogedor de las condensaciones de los frascos (Drip tray) ................................................................................. 41
8.4. Lyoquestlogger software ....................................................................................................................................... 42
9. INSTR CCIONES DE SO ..................................................................................................................... 43
9.1. Puesta en marcha .................................................................................................................................................. 43
9.2. Modo de funcionamiento semiautomático ........................................................................................................... 44
9.3. Modo de funcionamiento automático ................................................................................................................... 44
9.4. Modo de funcionamiento descarche ..................................................................................................................... 45
9.5. Menú ...................................................................................................................................................................... 45
9.6. Contador horas bomba de vacío ............................................................................................................................ 46
9.7. Parada del liofilizador ............................................................................................................................................ 46
9.8. ersión No Plus ...................................................................................................................................................... 47
10. RECETAS ............................................................................................................................................. 48
11. ALARMAS ........................................................................................................................................... 48
12. PLAN DE MANTENIMIENTO ................................................................................................................. 50
12.1. Mantenimiento del grupo de vacío ....................................................................................................................... 50
12.2. Mantenimiento del grupo frigorífico ..................................................................................................................... 52
12.3. Limpieza y desinfección superficial ........................................................................................................................ 52
12.4. Tabla de mantenimiento ........................................................................................................................................ 52
13. ANOMALÍAS DE F NCIONAMIENTO .................................................................................................... 53
13.1. Bomba de vacío ...................................................................................................................................................... 53
13.2. Grupo frigorífico ..................................................................................................................................................... 54
13.3. Conjunto liofilizador ............................................................................................................................................... 54

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14. ANNEXES /ANEXOS ............................................................................................................................. 55
14.1. Equipment spare part codes / Códigos de los recambios de la instalación ........................................................... 55
14.2. Examples of recipes / Ejemplos de recetas ............................................................................................................ 56
14.3. Maintenance records / Histórico de mantenimiento ........................................................................................... 58
14.4. Decontamination declaration / Declaración de descontaminación ...................................................................... 60
14.5. Refrigerating system P&ID / P&ID Sistema de refrigeración (Lyoquest -55) ......................................................... 61
14.6. Refrigerating system P&ID / P&ID Sistema de refrigeración (Lyoquest -85) ......................................................... 62
14.7. acuum system P&ID / P&ID Sistema de vacío ..................................................................................................... 63
14.8. Electric diagrams / Esquemas eléctricos (Lyoquest -55) ........................................................................................ 64
14.9. Electric diagrams key / Leyenda esquemas eléctricos (Lyoquest -55) ................................................................... 69
14.10. Electric diagrams / Esquemas eléctricos (Lyoquest -85) .................................................................................... 70
14.11. Electric diagrams key / Leyenda esquemas eléctricos (Lyoquest -85) ............................................................... 75
14.12. Warranty certificate / Certificado de garantía ................................................................................................... 76
14.13. Declaration of conformity / Declaración de conformidad ................................................................................. 77
14.14. Accessories configuration / Configuraciones de accesorios ............................................................................... 78
14.15. LyoQuest standard codification / Codificación estándar LyoQuest ................................................................... 79

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1. GENERAL DATA
This instruction manual is valid for the laboratory freeze-dryers models:
LYOQUEST -55 (PLUS / NO PLUS)
LYOQUEST -85 (PLUS / NO PLUS)
C UTION
•The readin and comprehension of this manual are mandatory before the start up of the freeze-dryer.
•All safety instructions that are mentioned in this manual must be respected.
•Before any maintenance operations can be in the freeze-dryer must be disconnected from the power supply.
•A use or an application not accordin to the recommendations expressed in the manual can enerate a risk
situation and means the loss of the manufacture uarantee.
The European Union issued the Directive 2012/19/EU on Waste of Electrical and Electronic Equipment (WEEE).
This product is required to comply with the European Union’s Waste Electrical & Electronic
Equipment (WEEE) Directive. Mark symbol is shown at the ri ht.
Azbil Telstar sells products throu h distributors throu hout Europe. Contact your local distributor for
recyclin /disposal.
The name plate & CE mark sticker is located at the back of the freeze-drier.
M NUF CTURER: ZBIL TELST R TECHNOLOGIES, S.L.U.
Av. Font i Sa ué, 55
Parc Científic i Tecnolò ic Orbital 40
08227 TERRASSA (SPAIN)
Telf ([phone]
Fax [phone]
e-mail: [email protected]
http://www.telstar.com

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2. DETAILS OF THE FREEZE-DRYER
2.1. AIM OF THIS MANUAL
The main aim of this manual is to act as a uideline for the handlin , operation and maintenance of the equipments
LYOQUEST -55 and LYOQUEST -85.
2.2. APPLICATION
This model of freeze-dryer can be used for the freeze-dryin in flasks, vials, ampoules or in bulk, by means of the
different confi urations of the basic unit and the possibility of couplin several accessories.
W RNING
•This freeze dryer is NOT a sterilizable unit.
•This freeze dryer consists of methacrylate parts. Not followin the instructions about the disinfection of the unit
(chapter 12.3) or in the event of freeze dryin methacrylate corrosive products can dama e the parts, bein
excluded of the uarantee of the unit.
•Under no circumstances may the unit be used with corrosive, flammable or explosive ases.
•In the event of freeze dryin products containin corrosive or explosive solvents, please contact the Customer
Service, to take the specific preventive actions for the specific product.
•Certain freeze-dryin cycles increase the required preventive maintenance actions on the vacuum pump. See
chapter 12.1.
2.3. REQUIREMENTS
Electrical supply: 230V monophase + PE, 50 Hz / 230V monophase +PE, 60 Hz
Installed power: LYOQUEST –55: 2.5 kW
LYOQUEST –85: 3.5 kW
Consumption in amperes: LYOQUEST –55: 7A (11A max)
LYOQUEST –85: 9A (15A max)
Maximum environmental temperature to work: 25ºC
2.4. DESCRIPTION
The LYOQUEST freeze-dryer is constructed as a compact desktop version. The basic unit consists of a metallic box
with lateral access doors to perform maintenance tasks. The vacuum pump is supplied separately and is optional.
The control panel is located at the front.
LYOQUEST components:
•A two-sta e vacuum pump provided with a as ballast or a bubbler ( as pur in apparatus). When the pump is
switched off a closin valve isolates the pump from the freeze-dryer, preservin the latter’s vacuum while
atmospheric pressure is re-established in the interior of the vacuum pump, so backlash of oil is avoided. An
exhaust filter is also included.
•A refri eratin system, which cools the condenser coil to the workin temperature. The condensation surface
has been dimensioned ensurin the condensation of the water vapor released by the products that are bein
freeze dried. This prevents vapors from enterin the vacuum pump. The refri eratin system is formed by:
- Compressor:
LYOQUEST-55: one-sta e hermetic compressor, condensed by air.
LYOQUEST-85: two one-sta e hermetic compressors, condensed by air.
- Condenser:
Material: AISI 316L stainless steel.
LYOQUEST models:
•PLUS: Prepared to work with all accessories and heated plates.
•NO PLUS: Prepared to work with all accessories, but not with heated plates.

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1. Manifold with 8 inlets 5. Connection RS232 for Lyolo er
2. Touch screen 6. Vacuum pump
3. Drainin 7. Electrical connection of the vacuum pump
4. Electrical connection and switch 8. Chamber with heated plates
Fi . 1
1
2
3
4
5
6
7
8

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3. TECHNICAL CHARACTERISTICS
V CUUM PUMP (optional) ULVAC GLD 136C ILMVAC P6Z-101
Dimensions (len th x width
x
hei ht)
mm 487.5x170x249.5 500 x 300 x 340
Wei ht k 27 25.4
Suction flow (50Hz/60Hz) m
3
/h 8.1/9.72 5.8/6.6
Vacuum at the pump inlet mbar See user manual of the pump
Motor velocity r.p.m. - 1500
Motor power kW 0.4 0.4
Oil
Capacity liters 1 1
Type (standard
applications) AV-30 LABOVAC 10
ICE CONDENSER LYOQUEST -55 LYOQUEST -85
Condensation surface m
2
0.25
Minimum temperature (T
room
≤ +21ºC) ºC -55 -85
Ice capacity k 8 (4 k /24 h) 8 (6 k /24 h)
Material AISI – 316L
REFRIGER TING UNIT LYOQUEST -55 LYOQUEST -85
Number of compressors 1 2
hermetic type One sta e
Refri eration power (at -35ºC) W 495 800
Refri erant (free of CFCs) R-507 R-507/ R-23
Refri eration of the unit By air circulation
EQUIPMENT LYOQUEST -55 LYOQUEST -85
Electrical connection V, ph, Hz 230, 1, 50 / 230, 1, 60
Total installed power kW 2.5 3.5
Process vacuum. Unloaded, T
room
≤ 21ºC,
condenser temperature: -55ºC (LYOQUEST -55),
condenser temperature: -85ºC (LYOQUEST -85)
mbar < 5 x 10
-2
< 3 x 10
–2
Dimensions
(hei ht x width x depth) mm 480 x 425 x 575
Vacuum au e DIGITAL
PT100 Temperature probe DIGITAL
Wei ht k 65 70

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4. TRANSPORT AND PACKING
The freeze-dryer is delivered to ether with the optional accessories and documentation in a sin le pack; it is packed in
a suitable way to prevent it from bein dama ed durin transportation. The freeze-dryer and the accessories are also
packed in an individual packa e.
The pack includes a tube of silicone rease, to carry-out the maintenance of the equipment seals.
The unit has to be shipped in vertical position accordin to the crate indications (adhesive labels).
After havin unpacked the freeze-dryer, it is necessary to verify that the pack includes all the optional accessories
shown in the order.
5. BEFORE START-UP
Before proceedin with the start-up of the freeze-dryer the followin instructions must be followed step by step.
5.1. PLACING ON SITE
The unit must be placed on a table that has a smooth and horizontal surface. The basic unit must be firmly settled to
prevent vibrations.
W RNING
•When assemblin the chamber to the Lyoquest, do not for et to assembly the methacrylate base. It is required for
proper workin when chamber is installed.
5.2. ELECTRICAL CONNECTION
W RNING
•Before switchin the Freeze-Dryer on, check that the supply volta e is the same as the one mentioned on the
specification plate.
•Installation in the place where the Freeze-Dryer is connected to must meet the safety re ulations in force and be
accordin to the local re ulations of the country or customer facilities.
Connect the cable of the vacuum pump to the basic unit.
Connect the electrical cable of the basic unit to the power supply (the plu wire is supplied with a European plu ).
5.3. VACUUM PUMP
ULVAC GLD-136C / ILMVAC P6Z-101 vacuum pumps (Fi . 2 & 3):
The pump is supplied with the oil loaded. Verify the oil level (see section Vacuum unit maintenance).
In order to prevent the non-condensable vapors of the product of contaminatin the oil of the pump, the system has
Gas-Ballast function (see section Vacuum unit maintenance).
The vacuum pump is equipped with an exhaust filter, whose function is to retain oil vapors.
The electrical connection of the vacuum pump has to be connected to the base placed at the side of the unit.
Furthermore, the vacuum pump admission has to be connected to the basic unit throu h the supplied pipe.
Connection of the vacuum pump expulsion:
To direct the vapors outside of the pump, connect the pipe to vacuum pump exhaust, removin the installed filter.

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ULV C GLD-136C ILMV C P6Z-101
Fi . 2 Fi . 3
5.4. REFRIGERATING UNIT
In order to assure a suitable refri eration of the refri eratin unit (refri erated via forced air circulation by means of a
fan), all vents must be separated by at least 20cm from other equipment or the walls.
. RECOMMENDATIONS
.1. ICE CONDENSER
Before each operation it is necessary to open the water drain valve of the condenser in order to assure that the ice
condensed durin the previous operation has been totally eliminated. If water residues remain in the condenser or the
drain pipe, they will be frozen as a result of the vacuum, thus possibly compromisin work conditions.
.2. GREASING
Sli htly rease the airti ht seals with silicone rease. The contact surfaces should always be kept clean and reased.
Avoid dirt accumulation.
.3. LONG STOPPAGE
If the freeze-dryer is stopped for a lon period of time, the oil of the vacuum pump must be chan ed to ensure that
there is no corrosive waste resultin in dama e. Disconnect the unit from the power supply. Clean all the components
of the equipment (manifold, chamber...), check that the condenser has been completely emptied and rease the
airti ht seals of the chamber, valves and condenser.
Filter
Filter

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7. WORKING PRINCIPLE
7.1. FREEZE
The product inserted into the equipment can either be previously frozen or can be frozen in the ice condenser. To
freeze it in the condenser, the product is placed into the plate stack (optional accessory), and the condenser is cooled
selectin the option "freezin " on the touch screen, both in semiautomatic mode and in automatic mode.
The freeze temperature is proportional to the solidification point of product. Freezin speed influences the size of the
crystals and, consequently, sublimation speed. Therefore, the most suitable freeze parameters for each product must
be determined. It is advisable to freeze products at a temperature below -20°C for a period of 3-4 hours.
7.2. LYOPHILIZATION
7.2.1. IN MANIFOLD
It is used to lyophilize bulk products in lass flasks with a rindin mouth (29/32mm Ø). The products contained in the
flasks (fillin a quarter up to maximum half the flask) have to be previously con ealed in a rotatin bath of alcohol,
freezin the product to the walls of the flask.
When the condenser has reached the pro rammed temperature, the followin steps must be performed:
1. Connect the vacuum unit.
2. Connect the flasks or bottles (with previously frozen product) to the valves connected to the condenser.
3. Open the valves (see Fi . 4).
Then the vacuum is done inside flasks, and the sublimation of the product starts. A pressure of less than 10mbar is
reached within 10 minutes. If this does not happen, the pump will stop and the alarm of the vacuum system will
appear. In this case the valve-flask connections must be reviewed in order to assure that there is no a leak.
CONNECTION VALVE
Air inlet Start vacuum process
Fi . 4
If it is necessary to connect additional flasks when the freeze-dryin process has already be un, the followin steps
must be performed:
1. Close all valves.
2. Connect the new flask and open its correspondin valve.
3. Wait 10-15 seconds in order for the pressure to be restored in the unit.
4. Open the valves of the other flasks.
It is possible to provide external heat by means of an electric heat source, in order to speed up the dryin process. The
heat source should be at the suitable distance to apply the radiation heat that the product requires. A hot air heater
directed directly to the flasks may also be used.
At the be innin of the process a layer of ice will form on the outside wall of the flask; this will radually disappear as
dryin out continues. When the exterior of the flask is at ambient temperature, no ice remains in the product (the
process has finished). Once the process has finished, the correspondin valves of the manifold are closed and air
enters in the flasks. So they can be removed.

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7.2.2. IN CHAMBER
The product may be freeze-dried inside the condenser or in the chamber:
•Inside the condenser (see Fi . 5): It is advisable to load the product only on the lower and middle plates.
The hi her plates do not receive cold as do the other plates because the vacuum supply is placed at top of the
condenser. The equipment allows you to pro ram recipes (see chapter 10) to run a complete cycle without
makin any manual actions.
•Outside the condenser: This is recommended when the quantity of product allows you to load the three
plates. It is advisable to freeze the product inside the condenser, because the heated plates will be cold and
heatin rates from ne ative temperatures may be reached. Once the product is frozen, remove the plates of
the condenser and place them in the chamber (see Fi . 6). Also it is possible to load a product that has been
frozen in other equipment. The equipment allows you to pro ram recipes (see chapter 10) to run a complete
cycle without makin any manual actions once the product is frozen and the chamber has been placed.
NOTE
Fi . 5 Fi . 6
•The product layer should be as thin as possible (recommended hei ht <20mm). The time of the freeze-dryin
process depends for an important part from this hei ht. It is very advisable to load the freeze-dryer uniformly
and that the hei ht of product is the same in all zones.
•The time of the process also depends on:
-The heat provided to product (external if the manifold is used or by means of an electrical resistor placed
inside the plates).
-The maximum temperature accepted by the product.
-The final de ree of humidity desired.
-The percenta e of solid material in the product.
-The vacuum de ree obtained.
-The total amount of product to be dried each time.
-
The temperature of the condenser.

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7.2.3. END OF THE PROCESS
Press STOP to stop the pump and the refri eratin circuit. Open the air inlet of the valve on the side of the chamber.
The air inlet has to be opened very slowly to avoid the formation of turbulences that will spread about dust in the flasks
or plates. Next, the defrostin function is activated.
At the end of the process, the pump and the refri eratin unit will stop (the compressor stop is delayed in order to
extract all ases from the circuit). Next, a connection valve of the manifold or the ventin valve placed at the top cover
has to be opened in order to ventilate the inside of the equipment.
It is necessary to connect a container to the outlet of the outflow pipe. Once this is done the manual outflow valve
placed at the left side can be opened. Start defrostin mode (see chapter 9.4) in order to heat the surface of the
condenser which allows you to extract the ice block in a few minutes. Also it is possible to heat the condenser until the
entire ice block has been defrosted and all the water has been drained throu h the outflow valve. In both cases it is
advisable to remove the chamber or the manifold to accelerate the defrostin .
Before the start of a new operation, check that the condenser has been totally defrosted, that there are no water traces
in the overflow pipe, and that the overflow valve is closed a ain.
7.3. LYOPHILIZATION MODES
There are two possible lyophilisation modes: the AUTOMATIC mode (see chapter 9.3.) and the SEMIAUTOMATIC mode
(see chapter 9.2.). The automatic mode allows you to establish the processes, the parameters and the time by means
of a RECIPE (see chapter 10).
7.4. SELECTABLE PROCESSES
Freezing: It starts up the refri eratin system and cools the condenser. The coolin system requires a preparation
time (5 minutes approx.) in which the temperature may increase sli htly. After this the temperature will quickly
decrease. This step must be lon er than 30 min to make sure that the system can reach the minimum temperature.
Cool+Vacuum: It starts up the refri eratin system, cools the condenser and starts up the vacuum unit. The vacuum
be ins when the temperature of the condenser is lower than the established parameter (normally -40ºC). The control
of the vacuum level will depend on the Set Point pro rammed. The system will need a time lon er than 20 min to
reach the minimum vacuum level.
Heat plates: It starts up the electrical resistors of the plates. They warm up until the pro rammed temperature has
been reached. Also it starts up the vacuum unit. The vacuum be ins when the temperature of the condenser is lower
than the established parameter (normally -40ºC). The control of the vacuum level will depend on the Set Point
pro rammed.
Stop: It stops the system.
Each one of the processes may have parameters to set. In the automatic cycle it is also necessary to set the time for
each step. The parameters are the followin ones:
Semiautomatic utomatic
Freezing - Time (HH:mm)
Cool + vacuum Vacuum (mBar) Vacuum (mBar)
Time (HH:mm)
Heat plates Vacuum (mBar)
Plates temperature (ºC)
Vacuum (mBar)
Plates temperature (ºC)
Time (HH:mm)
Stop - -
When the equipment starts up, the compressor 1 and the refri eratin fans will start up. A few seconds later, the
compressor 2 will also start up (Lyoquest -85).
Dependin on the temperature of the condenser probe, the electrically-operated valve of the refri eratin circuit may
take a certain time to start workin . For this reason it is probable that the temperature increases some de rees
initially.
The pump starts to work when the Set Point has been reached, and once it works there is a small delay in openin the
vacuum valve.

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8. OPTIONAL ACCESORIES (COMPILATION AT THE END OF THE MANUAL)
8.1. CHAMBERS
Material: Methacrylate (PMMA)
The vial stopperin devices under vacuum are manual and vials provided with closure are required.
Fig. Description Diameter Distance between plates
7a Cylindrical chamber equipped with 3 standard plates 220mm 70 mm standard
7b Cylindrical chamber equipped with 3 heated plates 220mm
70 mm standard
/
140 mm lar e spacer (LS)
8 Cylindrical chamber equipped with 3 heated plates
and 8 inlets manifold 220mm
9 Cylindrical chamber equipped with 3 heated plates,
vial stopperin device and 8 inlets manifold 220mm
10 Cylindrical chamber equipped with 3 heated plates
and vial stopperin device 220mm
11a Bi size cylindrical chamber equipped with 3 heated
plates 350mm 71 mm standard
/
142 mm lar e spacer (LS)
11b Bi size cylindrical chamber equipped with 3 heated
plates and vial stopperin device 350mm
12a Bi size cylindrical chamber equipped with 4 heated
plates 350mm
71 mm standard
12b Bi size cylindrical chamber equipped with 4 heated
plates and vial stopperin device 350mm
Fi . 7a Fi . 7b Fi . 8 Fi . 9
Fi . 10 Fi . 11a Fi . 11b Fi . 12a Fi . 12b

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8.2. MANIFOLDS
Manifolds with 8-12-16 inlets
Horizontal manifold. Each one of the manifold inlets is provided with a 3 way valve which allows isolatin and
restorin the pressure in each one of the flasks independently. The valves allow the junction to rindin flasks by
means of the conical adapter that they incorporate (external diameter: 29-32 mm; internal diameter 18 mm). By
removin this adapter, tubes or flasks can be inserted directly in the valve.
Circular manifold with 8 inlets (fi 13a) and strai ht one with 12 inlets (fi 13b).
By joinin to ether two 8 inlets manifolds it is possible to et 16 inlets (fi 13c).
Fi . 13a Fi . 13b Fi . 13c
Manifolds with 40-80 inlets
Manifold with 40 inlets for 13mm diameter pipes. By means of a distributor it is possible to et 80 inlets (fi 14b).
Fi . 14a Fi . 14b
8.3. FLASKS CONDENSATIONS COLLECTOR (DRIP TRAY)
Cover to collect the condensations enerated on the outer part of the flasks of the manifold (Fi . 15).
Fi . 15

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8.4. LYOQUESTLOGGER SOFTWARE
Software for monitorin on PC (Fi . 16) that provides data for the vacuum and the temperatures of the condenser,
plates and product.
The equipment is connected by a RS-232 serial port on PC and it sends information in real time.
A data packet is sent every second in order to know the freeze dryer state.
The serial port is automatically confi ured by the software.
The dataflow is unidirectional from the freeze dryer to the PC. It means that it is only possible to monitor, and not to
control, the equipment connected to the PC.
Minimum computer requirements:
-Intel Pentium II o ADM Athlon Processor.
-512 MB of RAM.
-Operatin system: Windows XP, Windows 2000 o Windows 7.
-Fast IDE Hard Drive with more capacity than 5GB.
-SVGA Graphics card (32 bits colour at 1024 x 768 Pixels).
Fi . 16

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9. INSTRUCTIONS FOR USE
9.1. START UP
NOTE
To start up the equipment, press the switch placed above the electrical connection (see Fi . 17), movin it to the
position I.
The equipment will start up and the introduction screen will appear (Fi . 18).
Press on the middle of the screen to access to main screen (Fi . 19).
The main screen contains:
1. The condenser temperature value and the vacuum value. Press on this box to access the temperature probes
screen (Fi . 20).
2. An indicator of workin mode.
3. Four buttons, from left to ri ht:
-Access the automatic mode screen
-Access the semiautomatic mode screen
-Start defrostin mode
-Access the menu screen
The first three buttons are used to start a workin mode, for this reason they are disabled while the other mode is
activated.
Fi . 17 Fi . 18 Fi . 19 Fi . 20
Before be innin a new operation:
•Open the drain valve in order to check that all water from the last
operation has been totally eliminated. Close the valve a ain.
•Check that the condenser is covered by its transparent cover (manifold
or chamber).
•Check that all valves of the chamber are closed correctly.

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9.2. SEMIAUTOMATIC MODE
The second button of the main screen leads to semiautomatic mode (Fi . 21).
On the semiautomatic mode screen there is a selector to select the process to
realize.
•Stop
•Freezin
•Cool+Vacuum
•Heat plates
(see the selectable processes description in chapter 7.4)
Once activated, the system will reach the preset temperature and pressure as
soon as possible. Since the semi-automatic cycle does not allow to pro ram time,
the functions will not be time-dependent and the parameters will remain constant
until the equipment is switched off.
The temperature set point button is used to establish the temperature that the
plates will reach in the “heat plates” process.
The vacuum set point button is used to establish the vacuum value in the
processes “heat plates” and “cool+vacuum”.
The lower ri ht button is used to return to the main screen.
9.3. AUTOMATIC MODE
The automatic mode requires that a recipe has been added previously to the PLC (see chapter 10).
The first button of the main screen leads to automatic mode (Fi . 22).
On the automatic mode screen there are indicators: set points, step number of automatic cycle, process type and
process time. On the lower part of the screen there are four buttons, from left to ri ht:
•Start automatic cycle
•Stop automatic cycle
•Access the recipe in PLC screen
•Return to main screen
Pressin the third button, the recipe that will be followed appears (Fi . 23). The parameters of this recipe may be
modified even when the cycle has be un. The process to follow in each step and the parameters of the step that is
on oin may not be modified. Use the button (arrow) located in the upper ri ht or left corner of the screen to move
from the screen Fi . 23 (steps 1 to 5 of the recipe) to the screen Fi . 23a (steps 6 to 10 of the recipe) and vice versa.
To edit step by step, the edit button has to be pressed.
The lower ri ht button is used to return to the previous screen.
Fi . 22 Fi . 23 Fi . 23a
Fi . 21

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On the step by step edition screen (Fi . 24) the parameters of each step may be
edited.
The lower left buttons are used to access the previous and subsequent step. The
lower ri ht button is used to return to the recipe in PLC screen.
Dependin on the selected process in each step, the system allows to set a few
parameters.
To set each one of the parameters, the correspondin box must be pressed. A
numeric keypad will appear on the screen (Fi . 25).
The fixed parameters are final values of the step, that is, the values that the system
tries to reach at the end of the step. The system follows a few rates takin as initial
values the readin s of the probes when the step starts.
In the first vacuum step, the system doesn’t follow rates. The equipment will try to
reach the vacuum value durin the entire step.
9.4. DEFROSTING MODE
The third button of the main screen is used to activate and deactivate the defrostin
mode. After realizin a freeze-dryin cycle, it is possible to run a defrostin cycle in
order to defrost the ice accumulated in the condenser and to extract the water by the
drain (see Fi . 26). To perform the defrostin , heated as flows by the condenser coil
to increase its temperature.
The end of the defrostin mode takes place automatically, when a predetermined
time has passed.
9.5. MENU
The fourth button of the main screen is used to access the menu (Fi . 27). The menu
is used to pro ram a recipe, to visualize an alarm (if an alarm is activated) and to
access the adjustable parameters.
On the menu screen there are two buttons in the middle. The first of them leads to
the recipes edition screen (see chapter 10). The second button leads to the alarm
screen (see chapter 11). There are also four buttons on the lower area, from left to
ri ht:
•Access the settin s screen
•Access the synoptic screen
•Access to vacuum pump operatin hours counter
•Return to the main screen
On the settin s screen (Fi . 28) the safety temperature may be modified. This screen
contains boxes to select the use of the manifold or the plates. The three lower
buttons, from left to ri ht, are used to:
•Access the lan ua e selection screen
•Access the screen of screen adjustments
•Return to the main screen
Fi . 26
Time (hh:mm) Vacuum (mBar) Plates temp. (ºC)
Freezin
Cool + Vacuum
Heat plates
Stop
Required
Drai
n valve (opened)
Fi . 25
Fi . 27
Fi . 24