UNISENSE MultiChannel UniAmp User manual

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MICROPROFILING SYSTEM USER MANUAL

Microprofiling systeM user Manual
Copyright © 2023 · Unisense A/S
Version November 2023

MICROPROFILING SYSTEM USER MANUAL
UNISENSE A/S
TABLE OF CONTENTS
WARRANTYANDLIABILITY...........................................6
CONGRATULATIONS WITH YOUR NEW PRODUCT! ....................7
Support, ordering, and contact information 7
OVERVIEW..........................................................9
MICROSENSORAMPLIFIERS.........................................10
MANUAL MICROMANIPULATORS .....................................11
SpecificationS (mm33 and mm33-2)...................................11
MOTORIZEDSYSTEMS.............................................. 12
SpecificationS for motor controller mc-1, mc-2, and mc-3 13
SpecificationS for motor Stage mS-15 13
motor control Button - functionS 13
MICROMANIPULATORSTANDS...................................... 14
SpecificationS laBoratory Stand lS: 14
SpecificationS inSitu Stand iS19 14
SETTINGUP........................................................ 15
ASSEMBLY PROCEDURE 1D MOTORIZED SYSTEM . . . . . . . . . . . . . . . . . . . . . 17
ASSEMBLY PROCEDURE 2D MOTORIZED SYSTEM .................... 19
ASSEMBLY PROCEDURE 3D MOTORIZED SYSTEM .................... 21
STORAGE AND MAINTENANCE ......................................25

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notice to purchaSer
This product is for research use only. Not for use in human diagnostic or
therapeutic procedures.
Warning
Microsensors have very pointed tips and must be handled with care to avoid
personal injury and only by trained personnel.
Unisense A/S recommends users to attend instruction courses to ensure proper
use of the products.
Warranty and liaBility
The MicroProfiling System is covered by a one year limited warranty.
The warranty does not include repair or replacement necessitated by accident,
neglect, misuse, unauthorized repair, or modification of the product. In no event
will Unisense A/S be liable for any direct, indirect, consequential or incidental
damages, including lost profits, or for any claim by any third party, arising out of
the use, the results of use, or the inability to use this product.
Unisense mechanical and electronic laboratory instruments must only be
used under normal laboratory conditions in a dry and clean and environment.
Unisense assumes no liability for damages on laboratory instruments due to
unintended field use or exposure to dust, humidity or corrosive environments.
repair or adjuStment
Sensors and electrodes cannot be repaired. Equipment that is not covered
by the warranty will, if possible, be repaired by Unisense A/S with appropriate
charges paid by the customer. In case of return of equipment please contact us
for return authorization.
For further information please see the documents Conditions for Sale and
Delivery for Unisense and Warranty and Shipping Information as well as the
manuals for the respective products.
WARRANTY AND LIABILITY

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support, ordering, and contact inforMation
If you wish to order additional products or if you encounter any problems and
need scientific/technical assistance, please contact our sales and support
team. We will respond to your inquiry within one working day.
E-mail: [e-mail]
Unisense A/S
Tel: [phone]
Fax: [phone]
Further documentation and support is available
at our website www.unisense.com.
CONGRATULATIONS WITH YOUR NEW PRODUCT!
REPLACEMENT OF SENSORS
Unisense will replace sensors that have been damaged during shipment
provided that:
• The sensors were tested immediately upon receipt in accordance with the
delivery note and the manual
• The seal is still intact.
• The sensors are returned to Unisense for inspection within two weeks.
• The sensors are correctly packed for return to Unisense, in accordance with
the note included in the sensor box.

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OVERVIEW
With its stability, precision, and eminent spatial resolution, the MicroProfiling
System is an outstanding tool for microscale measurements for a variety of
applications.
The optimal choice of sensors and equipment depends on what kind of
experiment you want to perform.
You can perform manual or automated profiles. In manual profiles, the
micromanipulator is operated by hand. For increased accuracy and automatic
profiles, a motorized stage and motor controller can be used. This will increase
microsensor positioning precision, extend the travelling distance, facilitate
multiple automated profiles and free the scientist from the tedious manual
micromanipulator operation.
This manual gives an overview of the components of a Unisense profiling
measurement set-up and how to connect them. For details on each component,
please consult the individual manuals.

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MICROSENSOR AMPLIFIERS
It takes a specialized high-quality amplifier to amplify the signal from a
microsensor.
Amperometric microsensors (e.g. O2, H2S, H2) require either a versatile amplifier
with adjustable polarization like the MultiChannel UniAmp fx-6 or fx-3 or a
picoammeter with preset polarization like the O2UniAmp.
Potentiometric microsensors (e.g. pH and Redox) require a high-impedance
millivoltmeter like the pH/Redox UniAmp or a MultiChannel UniAmp.
All Unisense amplifiers have an integrated A/D-converter unit, so they are
connected to the computer directly.
Please see separate manuals for more detailed instruction and specifications.
MultiChannel UniAmp SingleChannel UniAmp

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MANUAL MICROMANIPULATORS
A microsensor must be positioned in the measuring environment with
much more precision than is possible to achieve with an ordinary
laboratory stand. Therefore, Unisense supplies manual micromanipulators
with holders for one (MM33) or two sensors (MM33-2), which facilitate
reliable measurements and minimize the risk of breaking the microsensor.
Mounted on a suitable laboratory stand (e.g. the Unisense LS), the
micromanipulator provides a robust and precise tool for positioning
microsensors with a z-axis precision of 10 microns. The x- and y-axis can
be manipulated with a precision of 100 microns.
The MM33-2 offers the possibility for simultanous positioning of two
sensors. It allows for extremely fine adjustment of the two microsensor
tips relative to each other so that the two sensors measure practically at
the same point.
specifications (MM33 and MM33-2)
• Weight: 1100 g (MM33), 1240 g (MM33-2)
• z-axis: 37 mm, resolution 100 µm
• 10 mm, resolution 10 µm
• 100 mm, resolution 0.5 µm, MMS
• 300 mm, resolution 0.5 µm, MMS-30
• y-axis: 20 mm, resolution 100 microns
• x-axis: 25 mm, resolution 100 microns
Micromanipulator with
two-sensor holder mounted
with sensors
Micromanipulator mounted on
a Motor Stage

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MOTORIZED SYSTEMS
For precision below 10 micron in the x-axis and for automated
measurements, the MM33 (or MM33-2) can be mounted on a
micromanipulator Motor Stage (MS-15), which is positioned via the PC
data acquisition and motor control software (SensorTrace Suite) and a
motor controller (MC-1, MC-2, or MC-3).
With multiple Motor Stages automated measurements can be performed
in 2D or 3D with extreme precision it all directions.
Motorized sensor positioning is superior to manual positioning due to
a higher level of precision, a longer operating distance, and ease of
operation. A motorized unit consists of a micromanipulator (MM33/
MM33-2) mounted on a mechanical Motor Stage (MS-15) via a small
adapter. The stage is controlled by PC software (SensorTrace Suite) via a
motor controller (MC-1, MC-2, or MC-3).
A manual positioning system can always be upgraded to a motorized
system. This requires a Motor Stage (MS-15) and a motor controller (MC-1,
MC-2, or MC-3).
Motor Controller

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specifications for uniMotor controller Mc-1, Mc-2, or Mc-3
• AC power supply adaptor: In: 100-240 VAC, 60-50 Hz, 1.4 A Out:
24VDC, 5A, 120 W max
• Interface: USB
• Congured for: UniMotor Stage 15 cm (MS-15)
• Operating temperature: 0-70°C (32 to 158°F)
• <90% RH non-condencing
specifications for uniMotor stage 15 cM Ms-15 (with Mc-1, Mc-2, or Mc-3)
• Movement in longitudinal axis: 150 mm
• Resolution of motorized micromanipulator stage: 0.5 µm
• Accuracy: 0.2%
• Maximum linear speed: 10 mm/sec
• Minimum linear speed: 1 µm/sec
• Maximum axial load: 5 kg (11 lbs)
• Motor type: Stepper motor
Motor control button - functions
The Control Button on the motor housing (see picture right) has several
functions:
• Rotate up or down to move the Stage Plate up or down. The motor
speed increases with time, so short press for slow and precise
movement, long press for fast and long movement.
• Pushing the wheel in has two functions:
o Emergency stop if the motor is running.
o Making the light on the Motor Controller blink for the channel this
motor is connected to. This is useful to identify the X, Y, and Z axes in
2D and a 3D setup.
Micromanipulator mounted on
a Motor Stage

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MICROMANIPULATOR STANDS
The LS is a highly stable, corrosion-protected laboratory stand, which
makes it possible to maintain the precision of the MM33, MM33-2 and the
motorized micromanipulator stage.
The in situ stand IS19 is a light-weight micromanipulator stand for field
use in soft substrates such as mud or sediments. It consists of a pointed
angular aluminum pole, and its tip is pushed into the substrate to
provide a robust support for micromanipulators and sensors during in situ
experiments. It can be used under water as well as in air.
specifications laboratory stand ls:
• Weight: 12 kg (26.5 lbs)
• Dimensions: 40 cm x 30 cm x 54 cm (15.7” x 11.8” x 21.3”) assembled.
• Surface treatment: anodized
specifications insitu stand is19
• Weight: 2.5 kg (5.5 lbs)
• Height: 70 cm (28”)
• Fixation holes: thread M6, spacing between holes 5 cm (2”)
• Surface treatment: anodized

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Motorized 1D profiling set-up with fx-6 UniAmp, one motor controller,
single sensor, CAL300 and computer (not provided)
SETTING UP
The following section will describe a typical profiling set-up.
Please consult separate manuals for the individual components for
further details.
1. Install the Unisense SensorTrace Suite software.
2. Place the laboratory stand LS on a stable and smooth surface.
Adjust the feet to prevent the stand from rocking. If the sample is
not to be placed on the stand plate, the stand should be placed
at an appropriate distance from the sample to allow space for the
micromanipulator.
3. Mount the micromanipulator or assembled motorized system
(see below for instructions) on the stand with the xation screw
at a suitable height so that the lower side of the sensor holder is
positioned about 12.5 cm/5” above the top of the sample, when the
microsensor z-axis is completely retracted.
4. If motorized, connect the motor port of the motor controller to the
motor stage(s) using the motor cable(s).
5. Connect the the motor controller to the PC using the USB (USB -
mini USB) cable. In the SensorTrace Proling software setup screen
each connected motor controller can be assigned to a specic axis.
Clicking the controller on the setup screen will cause the green light
on the motor controller to blink for identication. See the SensorTrace
Suite User Manual for details.
6. Place the microsensor amplier(s) near the stand (0.5m/1.5ft)
7. Connect the ampliers to the PC using the USB cable.
8. Place the sample to be investigated under the micromanipulator
9. Place a microsensor in the holder of the micromanipulator with the tip
a few centimetres above the sample. Move the sensor horizontally to
the desired location with the x- and
y-controls. Be careful not to break the
microsensor tip!
10. Make measurements with the sensors
according to the manuals for the
sensors and software.

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ASSEMBLY PROCEDURE 1D MOTORIZED SYSTEM
1. Connect the Motor Stage, MS-15, to the UniMotor Controller, MC-1,
and connect this to power.
2. Use the hex key to separate the Tilting Base from the Micromanipu-
lator itself (Fig. 1).
3. Mount the u-shaped spacer on the tilting base using the provided
bolts (Fig. 2).
4. Place the MS-15 on the u-shaped spacer (Fig. 3a & b). The Tilting Base
and Motor stage should be oriented as shown in Fig. 3. This reduces
the risk of sensor damage due to accidentally loosening the two nuts
holding the tilt function.
5. Mount the MS-15 on the u-shaped spacer with four bolts (red arrows
in Fig. 4). There are eight holes in the back of the MS-15 so the MS-15
can be mounted in one of three positions, centred on the Tilting Base
or high or low. To get access to the holes in the MS-15 back, move the
Stage Plate up or down using the Motor Control Button on the motor
housing (Fig. 5). Rotate the button up or down to make the motor
move the Stage Plate up or down to reveal the holes for the bolts.
Insert the bolts and tighten gently.
6. Mount the Micromanipulator, MM33 or MM33-2, on the Stage Plate
and x it using the bolts removed in step 2 above (Fig. 6).
7. Mount the MS-15 - Micromanipulator assembly on the Laboratory
Stand, using the Elongated Nut and the Knurled Bolt (Fig. 7). Put the
Knurled Bolt through the hole in the centre of the Tilting Base and
mount the Elongated Nut on the bolt a couple of turns. Slide the
Elongated Nut down the groove on the Laboratory Stand (Fig. 8).
Tighten the Knurled Nut using the metal rod (Fig. 9).
Fig. 1
Fig. 2
Fig. 3a
Fig. 3b

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Fig. 5 Fig. 6
Fig. 7
Fig. 8
Fig. 9
Fig. 4

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ASSEMBLY PROCEDURE 2D MOTORIZED SYSTEM
(BEFORE NOVEMBER 2023)
1. Follow step 1 and 2 from the 1D assembly procedure.
2. Mount one motor stage across the U-prole (see picture). Move the
stage plate completely down by turning the spindle joint with a nger
and mount two of the provided large screws in the two upper of the
four central holes in the back of the stage as shown below. Then
turn the spindle to move the stage plate completely in the opposite
direction and mount two of the provided large screws in the bottom
holes.
3. Place the second motor stage on the rst one. The second stage must
be perpendicular to the rst one. Orient the second stage so that
the motor points in the direction where the tilting plate is hinged (see
step 3 in the 1D assembly procedure above). Move the stage plate
completely down by turning the spindle joint with a nger and mount
two of the provided screws in the two upper of the four central holes in
the back of the stage. Then turn the spindle to move the stage plate
completely in the opposite direction to mount two of the provided
screws in the bottom holes.
4. The provided square plate has four holes: two center holes near the
middle and two corner holes near the corners. With the 1D MMS neck
(controller connection) pointing upwards, mount the plate with the
two center holes located just right of the middle. Use the large screws
(see picture under 1D motor mounting)
5. Mount the MM33 or MM33-2 on the plate using the two screws from
step 1 in the 1D assembly procedure.

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ASSEMBLY PROCEDURE 3D MOTORIZED SYSTEM
(BEFORE NOVEMBER 2023)
1. Mount the 3D arm on the vertical post of the Laboratory Stand. To do
this, put the aluminium nger bolts into the holes on the 3D arm and
mount the elongated nuts by one to two rounds. Slide the nuts into
the groove of the stand. Position the arm horizontally and tighten the
aluminium nger bolts well with the provided tool.
2. Mount the rst Motor Stage (MMS) on the 3D arm using
4 of the long 4 mm bolts. Move the moving stage on the
MMS to one side by rotating the spindle by hand. This
will expose two of the xing holes (see picture). Mount
the 2 bolts and move the moving stage to the other
side to expose the other two xing holes and mount
the bolts.
3. Mount the 3D angle on the rst MMS. This is xed
using two 6 mm bolts as indicated in the picture.
4. Mount the second MMS on the 3D angle using
two long 4 mm bolts (see picture). Make sure the
second stage is horizontal and tighten the bolts
well.
3D Arm
Moving stage
Fixing holes
Spindle
Fixing holes
3D Angle
Fixing holes

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5. Mount the micromanipulator on the third
MMS. First remove the tilting base from the
micromanipulator, mount the square plate on the
micromanipulator with 2 short 4 mm bolts. Then
mount the square plate to the moving stage of
the MMS with two long 4 mm bolts (see picture
and Assembly procedure 1D motorized system
above).
6. Mount the third MMS with micromanipulator on
the second MMS with the spindles on the two
perpendicular to each other (see picture). The third
MMS must be xed with 4 of the short 4 mm bolts.
To get access to the xing holes, move the moving
stage to one side, rotating the spindle by hand.
Then mount the two bolts and move the moving
stage to the other side and mount the last two
bolts.
7. Connect the motors to the motor controllers using
the supplied cables (see Setting up above)

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STORAGE AND MAINTENANCE
Unisense recommends that the micromanipulator, lab stand, and in situ
stand are protected with silicone spray before use. This will help to keep
the surfaces clean and will prolong the lifetime of the unit.
Rinse the micromanipulator and stands with distilled water if salt or other
corrosive material have been deposited on them.
Keep the micromanipulator and motor free of sand and dust.
WARNING
Do not spray the MMS with
silicone
IMPORTANT
If you encounter problems that
cannot be solved from the
troubleshooting sections of
the individual manuals, please
contact sales@unisense.
com for support (we will try
to answer you within one
workday)
UNISENSE, DENMARK
www.unisense.com · [e-mail]