PC-505B Manual, Rev. 201013
Cap Comp checkout............................................................................................................................24
Current clamp checkout .....................................................................................................................24
OPERATION............................................................................................................................................26
Initial settings........................................................................................................................................26
Preliminary ...........................................................................................................................................27
Mount the electrode............................................................................................................................27
Submerge the electrode tip.................................................................................................................27
Zero the pipette offset.........................................................................................................................27
Measure the pipette resistance (RP)...................................................................................................28
Voltage clamp........................................................................................................................................29
Attach to the cell and form gigaseal...................................................................................................29
Set electrode capacitance compensation............................................................................................30
Measure seal resistance (RS) .............................................................................................................30
Patch or whole cell recording?..........................................................................................................31
Patch recording - Leak subtraction....................................................................................................31
Patch recording –Excised patch........................................................................................................31
Patch recording - Cell-attached patch...............................................................................................32
Whole-cell recording –Initial conditions...........................................................................................32
Whole-cell recording - Rupture the membrane patch........................................................................32
Whole-cell recording - Series R compensation..................................................................................33
Whole-cell recording - % correction..................................................................................................34
Current clamp.......................................................................................................................................35
Switching into current clamp .............................................................................................................35
Recording in current clamp................................................................................................................35
External command currents ...............................................................................................................36
Bilayer recording..................................................................................................................................36
THEORETICAL CONSIDERATIONS .................................................................................................37
Chloriding silver wire...........................................................................................................................37
Chloriding by electrolysis ..................................................................................................................37
Chloriding chemically........................................................................................................................37
Electrode holders..................................................................................................................................37
Care and use of holders......................................................................................................................38
Cleaning and storage .........................................................................................................................38
Replacing holder parts.......................................................................................................................38
Reference electrodes.............................................................................................................................39
APPENDIX................................................................................................................................................40
Specifications.........................................................................................................................................40
Accessories and replacement parts.....................................................................................................42
Warranty and service...........................................................................................................................43
Warranty.............................................................................................................................................43
Service notes.......................................................................................................................................43
