Warner OC-725C User manual
Publication 5720-001-REV-1.0
Oocyte Clamp
Amplifier
User’s Manual
Warner Instruments OC-725C
1
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Table of Contents
SUBJECT
Warranty
Safety
Introducon
Unique Features
High Voltage Compliance
Bath Clamp Headstage
Dual Oocyte Studies
Voltage Headstage Probe
Voltage and Current Meters
Addional Features
Buzz controls
Electrode Test
Overload Alarm
Nomenclature
Control Descripon
Front panel
Voltage electrode
Bath electrodes
Commands
Current electrode
Rear panel
Addional components
Voltage recording headstages
Bath headstage
Current electrode cable
Model cell
Comments
High voltage outputs
Using the Model Membrane
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4
5
6-7
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7
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8
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9-15
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11
12
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14
14
14
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15
16-19
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Table of Contents
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20-26
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25
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27-30
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SUBJECT
Inial instrument sengs
Test procedures
Voltage electrode test
Buzz
Current electrode test
DC clamp test
AC clamp test
Setup
Electrode holders
Bath probe
Electrode placement and grounding
Bath clamp electrode placement
Single oocyte setup with indirect ground
Single oocyte setup with direct ground
Dual ooctye set-up
Using the gain select
A Procedure for Recording from Oocytes
Voltage electrode placement
Current electrode placement
Impaling the cell
Clamping the cell
Clamping high conductance cells
Unclamping the cell
Removing the electrodes
Special Circumstances
High side current measuring
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Table of Contents
PAGE #
32-33
32
32
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34-37
34
36
36
36
37
SUBJECT
Comments and Recommendaons
Membrane damage
Repeated recordings
Electrophysiology
Appendix
laboratory environment
Gain telegraph outputs
4
Serial Numbers
Calibraons
Warranty
This warranty extends only to the original customer purchaser.
IN NO EVENT SHALL WARNER INSTRUMENTS BE LIABLE FOR INCIDENTAL OR
Repair Facilies and Parts
CAUTION
This temperature controller is not registered with the FDA and is not
for clinical use on human paents. REFER TO SAFETY INFORMATION
AND SETTING UP THE HARVARD APPARATUS VENTELITE BEFORE
PLUGGING IN THE VENTILATOR.
Warranty & Repair Information
5
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Safety Information
To Prevent Hazard or Injury:
Use Proper Line Cord
Ground the Product
product is properly grounded.
Make Proper Connecons
to the unit must be no longer than 3 meters.
Observe All Terminal Rangs
Use Proper Fuse
Avoid Exposed Circuitry
Do Not Operate with Suspected Failures
Orient the Equpment Properly
Place Product in Proper Environment
Observe all Warning Labels on Product
Read all labels on product to ensure proper usage.
6
Introduction
Unique Features
High Voltage Compliance: The OC-725C combines high AC and DC gains and a voltage
subtracts the voltage drop across the series resistance in the bath.
membrane voltage Vmand membrane current Im
e
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Introduction
Addional Features
Electrode Test
Capacity Compensationmvoltage input.
Overload Alarm
DC Offsets
8
Nomenclature
Appendix B
Text Convenons
• Warner Instrument product numbers are presented using a bold type.
.
italic type.
slow, fast, 100 mV
• Special comments and warnings are presented in highlighted text.
THIS EQUIPMENT IS NOT DESIGNED NOR INTENDED FOR USE ON HUMAN SUBJECTS
9
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Control Description
and the current cable with electrode holder.
Front Panel
[Vm [Im. The instrument
and Ve
on/off and
Voltage Electrode
The the
m and
the Vm
are also located in
The reports the membrane
m
The is a 7-pin
voltage probe to the instrument.
The Vm control is a 10-turn
the
The Vm
Ω
mx10 output BNC
Ω
The Vm
The Vmx10
use as an electrometer in intracellular measurements. Input capacitance up to 45 pF can
this circuit.
10
Ω
Bath Electrodes
The
output BNC’s.
The e
switch is off
mV.
switch set to slow or fast
m
sensed by the bath electrode. A lit LED
Instrument gain is set by the two
controls. Gain is selected by a
0.1 to 10
x0.1x1.0 and
x10
0.01 to 100.
Control Description
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Control Description
Clamp Secon
SELECTOR switch as well as the GAIN and DC GAIN controls.
SLOW
with the model membrane
FAST
oocytes.
NOTE: All CLAMP controls are disabled by turning the CLAMP
MODE SELECTOR switch to the o posion. Be sure to switch this
control to the o posion before handling electrodes!
12
Control Description
Commands
The controls and
÷10 input BNC.
controls -
thumbwheel and toggle switch. Ranges
x1.0 or x2.0off is selected with the
associated toggle switch.
Current Electrode
The e
e
contains the indicator and a DIN connector
Ve
ex10
output BNC or on the
Ω] will be displayed on the meter by depressing the
when the switch is in the off
Ve - The Vm
lamp will light. An alarm will also sound when the rear panel switch is in
the on
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Control Description
Rear Panel
switch and instrument GROUNDS.
NOTE: Verify that the instrument is wired for the proper voltage
before connecng the line cord.
appendix.
alarm.
GROUNDS - Both CIRCUIT and CHASSIS grounding posts are located on the rear panel.
CHASSIS is common with the instrument enclosure and connected to earth through the
NOTE: For safe operaon, the ground pin on the power plug must
not be removed and the use of “cheater” plugs must be avoided.
Addional Components
Voltage Recording Headstages
NOTE: The outer shell is electrically driven at the input potenal.
The microelectrode holder mates directly to the 2 mm input pin on the probe body. A
a micromanipulator. The handle can be mounted either axially or perpendicular to the
probe body.
14
Control Description
7255DI Dierenal Probe (Oponal)
single ended 7250 PROBE.
Bath Headstage
The BATH PROBE is housed in a 2.8 x 3.5 x 4.2 cm
labeled I SENSE and I OUT. The case is electrically
connects to the control unit with a 6 pin connector
Current Electrode Cable
with the electrode holder and a 3-pin connector on the other end to mate with the
Model Cell
Comments
Connecng to Line Power
The model OC-725C is supplied with a 3-conductor power cord. One conductor provides
the instrument will be assured provided that the ground circuit in the power outlet is
wired correctly and is connected to earth.
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Control Description
NOTE: If the ground pin of the power cord is removed for any reason
the instrument chassis must be directly connected to earth ground
High Voltage Outputs
CAUTION!: The current clamp is capable of high power output
(10 mA @ ±180 V) and can cause serious injury if not properly
handled.
16
Using the Model Membrane
resistors.
Inial Instrument Sengs
Connect the model cell to the OC-725C as shown on its cover. Be sure to connect the
BNC and the I MONITOR BNC on the OC-725C to an oscilloscope.
Control Control block Seng
O
Vm
0.1 V/µA
x1.0
O
Out
CCW to detent o
00 mV
x1.0
O
Ve
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Using the Model Membrance
Test Procedures
would be within tolerance.
Oset Controls
in the same manner as the Vm OFFSET control. This control is adjustable when the
Voltage Electrode Test
18
Using the Model Membrance
Buzz
Current Electrode Test
DC Clamp Test
19
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Using the Model Membrance
AC Clamp Test
COMMAND IN ÷10 BNC. Monitor the Vm x10 and I MONITOR outputs on the
oscilloscope.
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