Warner OC-725C User manual

Publication 5720-001-REV-1.0
Oocyte Clamp
Amplifier
User’s Manual
Warner Instruments OC-725C

1
www.warneronline.com
Table of Contents
SUBJECT
Warranty
Safety
Introducon
Unique Features
High Voltage Compliance
Bath Clamp Headstage
Dual Oocyte Studies
Voltage Headstage Probe
Voltage and Current Meters
Addional Features
Buzz controls
Electrode Test

Overload Alarm

Nomenclature

Control Descripon
Front panel
Voltage electrode
Bath electrodes

Commands
Current electrode
Rear panel
Addional components
Voltage recording headstages
Bath headstage
Current electrode cable
Model cell
Comments

High voltage outputs
Using the Model Membrane
PAGE #
4
5
6-7
6
6
6
6
6
6
7
7
7
7
7
7
8
8
9-15
9
9
10
11
12
12
13
13
13
14
14
14
14
14
15
16-19

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Table of Contents
PAGE #
16
17
17
17
18
18
19
18
20-26
20
21
21
21
22
22
22
23
23
25
25
26
27-30
27
27
28
28
29
29
30
30
31
31
31
31
SUBJECT
Inial instrument sengs
Test procedures

Voltage electrode test
Buzz
Current electrode test
DC clamp test
AC clamp test
Setup

Electrode holders
Bath probe
Electrode placement and grounding
Bath clamp electrode placement
Single oocyte setup with indirect ground
Single oocyte setup with direct ground
Dual ooctye set-up


Using the gain select

A Procedure for Recording from Oocytes

Voltage electrode placement
Current electrode placement
Impaling the cell
Clamping the cell
Clamping high conductance cells
Unclamping the cell
Removing the electrodes
Special Circumstances
High side current measuring




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Table of Contents
PAGE #
32-33
32
32
32
34-37
34
36
36
36
37
SUBJECT
Comments and Recommendaons
Membrane damage
Repeated recordings
Electrophysiology
Appendix

laboratory environment
Gain telegraph outputs




4
Serial Numbers


Calibraons

Warranty





This warranty extends only to the original customer purchaser.
IN NO EVENT SHALL WARNER INSTRUMENTS BE LIABLE FOR INCIDENTAL OR















Repair Facilies and Parts



CAUTION
This temperature controller is not registered with the FDA and is not
for clinical use on human paents. REFER TO SAFETY INFORMATION
AND SETTING UP THE HARVARD APPARATUS VENTELITE BEFORE
PLUGGING IN THE VENTILATOR.
Warranty & Repair Information

5
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Safety Information



To Prevent Hazard or Injury:
Use Proper Line Cord


Ground the Product



product is properly grounded.
Make Proper Connecons

to the unit must be no longer than 3 meters.
Observe All Terminal Rangs

Use Proper Fuse

Avoid Exposed Circuitry

Do Not Operate with Suspected Failures


Orient the Equpment Properly


Place Product in Proper Environment

Observe all Warning Labels on Product
Read all labels on product to ensure proper usage.

6
Introduction



Unique Features
High Voltage Compliance: The OC-725C combines high AC and DC gains and a voltage




















subtracts the voltage drop across the series resistance in the bath.





membrane voltage Vmand membrane current Im
e


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Introduction
Addional Features


Electrode Test
Capacity Compensationmvoltage input.
Overload Alarm

DC Offsets

8
Nomenclature

Appendix B 
Text Convenons





• Warner Instrument product numbers are presented using a bold type.

.


italic type.
slow, fast, 100 mV
• Special comments and warnings are presented in highlighted text.

THIS EQUIPMENT IS NOT DESIGNED NOR INTENDED FOR USE ON HUMAN SUBJECTS

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Control Description






and the current cable with electrode holder.
Front Panel

[Vm [Im. The instrument
 and Ve
on/off and 
Voltage Electrode
The   the
m and 
the Vm
 are also located in

The  reports the membrane
m

The   is a 7-pin

voltage probe to the instrument.
The Vm control is a 10-turn

the 
The Vm

Ω
mx10 output BNC
Ω
The Vm

The Vmx10 

use as an electrometer in intracellular measurements. Input capacitance up to 45 pF can

this circuit.