X-Cite Exacte User manual

X-Cite®exacte User Guide

X-Cite® exacte User Guide
035-00283 Rev. 3
Made in Canada
Excelitas Canada Inc. 2016
All rights reserved
No part of this publication may be reproduced, transmitted, transcribed, stored in a
retrieval system or translated into any language in any form by any means without the
prior written consent of Excelitas Canada Inc. Every effort has been made to ensure
information in this manual is accurate; however, information in this manual is subject to
change without notice and does not represent a commitment on the part of the authors.
Excelitas Canada Inc.
2260 Argentia Road
Mississauga ON L5N 6H7 CANADA
Trademarks
X-Cite®, exacte, Intelli-Lamp®, and Closed-Loop Feedback are registered trademarks of
Excelitas Canada Inc. All rights reserved.
All other product names are trademarks of their respective owners.

Table of Contents
1. X-Cite®exacte Message Reference.......................................................................1
2. Introduction............................................................................................................2
3. Safety......................................................................................................................3
3.1 Glossary of Symbols...........................................................................................3
3.2 Safety Precautions .............................................................................................4
4. Getting Started.......................................................................................................7
4.1 System Components..........................................................................................7
4.2 Front Panel.........................................................................................................8
4.3 Rear Panel.........................................................................................................9
5. Installation/Set-up................................................................................................10
5.1 Installing Lamp Module.....................................................................................10
5.2 Inserting/Removing Light Guide .......................................................................13
5.3 Tips to Prevent Premature Degradation of Light Guides...................................15
5.4 Installing the Collimating Adapter .....................................................................16
6. Operation..............................................................................................................18
6.1 Powering Up/Powering Down...........................................................................18
6.2 Quick Start: Illuminating a Specimen................................................................19
6.3 Front Panel Button Functions...........................................................................19
6.4 LED Indicators..................................................................................................20
6.5 Display Format Reference................................................................................20
6.6 Output Intensity Modes.....................................................................................21
6.7 Adjusting Light Output......................................................................................21
6.8 Viewing Accumulated Lamp Hours...................................................................22
6.9 Closed-Loop Feedback™ (CLF).......................................................................22
7. Calibration/Radiometry (optional).......................................................................24
7.1 X-Cite®exacte Power Reading using the XR2100 Power Meter or XR2000;....24
7.2 X-Cite®exacte Calibration using the XR2100 (or XR2000) & Light Guide.........25
7.3 Calibrating X-Cite®exacte with the Objective Plane Power Sensor, XP750......25
7.4 To Clear a Calibration.......................................................................................26
8. Warnings and Alarms..........................................................................................27
8.1 Clearing Alarms................................................................................................28
9. External Control...................................................................................................29
9.1 Graphical User Interface (GUI).........................................................................29
9.2 Installation:.......................................................................................................29
9.3 Graphical User Interface Panel.........................................................................30
9.4 Icons and Functions: ........................................................................................31
9.5 Logging Stability Data.......................................................................................33
9.6 Logging Events.................................................................................................33
9.7 Exporting Logged Data.....................................................................................33
9.8 Shutter Control via Foot pedal..........................................................................34
9.9 Shutter control via TTL.....................................................................................34
9.10 Software Developer’s Kit (SDK) .......................................................................35
10. Troubleshooting...................................................................................................36
10.1 Error Codes...................................................................................................36

10.2 Power Up Failure ..........................................................................................37
10.3 Lamp Strike Failure.......................................................................................37
10.4 Low Light Intensity ........................................................................................38
10.5 Shutter Failure...............................................................................................38
10.6 LED Display Failure ......................................................................................38
10.7 Cooling Fan Failure.......................................................................................38
11. Care and Maintenance.........................................................................................39
11.1 General.........................................................................................................39
11.2 Liquid Light Guides .......................................................................................40
11.3 Lamps...........................................................................................................43
11.4 Replacing the Lamp Module..........................................................................44
11.5 Air Vents & Filters .........................................................................................45
11.6 Replacing the Air Filter..................................................................................47
11.7 Replacing the External Fuses........................................................................47
12. Technical Specifications .....................................................................................48
12.1 Electrical .......................................................................................................48
12.2 Environmental...............................................................................................48
12.3 Lamp.............................................................................................................49
12.4 Light Guide....................................................................................................50
12.5 General.........................................................................................................50
13. Regulatory............................................................................................................51
13.1 Product Safety and Electromagnetic Compatibility: .......................................51
13.2 China RoHS..................................................................................................52
14. Warranty...............................................................................................................53
15. Contact Information.............................................................................................54
15.1 Replacement Parts and Accessories.............................................................54

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X-Cite®exacte User Guide
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1. X-Cite®exacte Message Reference
Display
Message
Definition
Description
XXX
Intensity Setting (%)
Displays the iris setting, which gives relative intensity as
percentage of the lamp’s maximum output
XX.XX
Power Setting (watts)
Displays the power setting, which gives absolute
intensity in terms of watts
uXXX
Power Setting (microwatts)
Displays the power setting, which gives absolute
intensity in terms of microwatts
X.XXX
or
XXX.X
Power Setting (milliwatts)
Displays the power setting, which gives absolute
intensity in terms of milliwatts
XXXX.
Lamp Hours
Displays the “hours of use” accumulated by the lamp
Flashing:
“XXXX”
Warming Up
The lamp is warming up. Warm up time is
approximately 4 minutes ref section 6.1
bulb
Lamp Error
Lamp installed incorrectly /
Lamp did not strike. See section 10.3
cool
Lamp is too hot to strike
The lamp will automatically strike when it has cooled to
the optimum striking temperature. See section 10.3
Alternating:
old / bulb
Old Lamp
The lamp has accumulated over 2500 hours. Lamp may
be near end of life.
Alternating:
end / bulb
New Lamp Required
The lamp has reached end of life. The lamp will not
strike.
LOC
Front Panel Locked
All front panel buttons have been locked to prevent
settings from being changed.
SFI
Shutter Failure
The shutter has failed to return to home position. Unit
should be restarted. If the error repeats, contact Tech
Support.
E1
Lamp temperature is too high
Check ventilation filters and outlets to ensure that no
blockage exists. Restart unit and if problem persists
replace lamp. If problem continues contact Tech
Support
E2
Internal communication failure
Restart unit, if problem persists contact Tech Support
E3
Iris failed to go to home position
Restart unit, if problem persists contact Tech Support
E4
Internal hardware failure
Restart unit, if problem persists contact Tech Support
E5
Failure to communicate with Intelli-
Lamp®
Check Intelli-Lamp®connection and restart unit. If
problem persists try another lamp, if problem still persist
contact Tech Support.
E6
Lamp self extinguished
Let system cool and restart lamp. If it goes out again,
replace lamp. If new lamp continues to self-extinguish,
contact Tech Support
E7
Internal system error
Restart unit, if problem persists contact Tech Support
See Section 10 for troubleshooting.

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2. Introduction
The X-Cite®exacte is a revolution in fluorescence microscopy illumination. Like the
previous X-Cite®illuminators, the X-Cite®exacte has the convenience of pre-aligned,
long-life Intelli-Lamps®, easy installation and compatibility will all major microscope
brands. With additional features such as Closed-Loop Feedback™, intensity adjustment
in 1% increments, a high speed internal shutter, and a calibrated output option which is
compatible with the X-Cite®Optical Power Measurement System, the X-Cite®exacte
reaches a new level of performance that makes it the ideal choice for quantitative,
comparative, and live cell imaging.
Excelitas Technologies Corp. is a global technology leader focused on delivering
innovative, customized solutions to meet the lighting, detection and other high-
performance technology needs of customers. X-Cite®, formerly of Lumen Dynamics
(which was acquired by Excelitas Technologies Corp. in November 2013) offers the Life
Science and Analytical Instrumentation market a broad range of innovative lamp and
LED fluorescence illumination and measurement solutions.
The X-Cite®exacte is a high quality product manufactured in accordance with ISO 9001,
CE marked and certified to UL, China RoHS and CSA standards.
We suggest that you read this manual to discover all its features, and how to use them.
Thank you for choosing X-Cite®!

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X-Cite®exacte User Guide
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3. Safety
3.1 Glossary of Symbols
CAUTION - Risk of danger: consult accompanying documents
WARNING -Eye damage may result from directly viewing ultraviolet light –
protective eye shielding and clothing must be used at all times.
Input/Output Signals
Input Signal
CAUTION –Hot Surface

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X-Cite®exacte User Guide
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3.2 Safety Precautions
The X-Cite®exacte is equipped with two safety sensors to protect the user from
accidental UV exposure. In addition, please observe the following precautions during
use. This series of cautions, warnings and dangers relate to the operation and
maintenance of the X-Cite®exacte. They are also presented throughout this User’s
Guide where necessary.
Warning
Eye damage may result from directly viewing the light produced by the lamp used
in this product. Always use protective eyewear and turn the lamp off before
removing cover.
Caution
Never look into the light emitting end of the light guide. The light could severely
damage the cornea and retina of the eye if the light is observed directly. Eye
shielding must be used at all times as well as clothing to protect exposed skin.
Warning
Always make sure the light guide is properly inserted into the X-Cite®exacte and
the microscope prior to turning on power to the unit. This will minimize the risk of
exposure to the UV light.
Caution!
To prevent damage/ degradation of the light guide, always allow adequate
clearance at the front of the X-Cite®exacte unit to prevent kinking or excessive
bending.
Warning
To reduce the risk of fire or shock, always replace the fuses with the same type
and rating.
Warning
Disconnecting of main supply source is only possible by unplugging the power
cord.
Danger
This unit contains HIGH VOLTAGE components. It is recommended that ONLY
QUALIFIED TECHNICAL PERSONNEL perform any testing or repairs described in
this manual. Disconnect the AC power cord from the unit before opening the cover
of this unit. All cover screws must be replaced prior to applying power to the unit, or
safety of the unit will be impaired.

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Monitoring the unit during manual operation
The level of UV and visible energy supplied by the X-Cite®exacte is sufficient to
ignite flammable substances. During manual operation, the unit must be attended
at all times by a qualified operator. The unit must not be left unattended while
turned on. If an operator leaves the work area of the unit, the lamp power switch
must be turned off.
Monitoring the unit during Automated operation
The level of UV and visible energy supplied by the X-Cite®exacte is sufficient to
ignite flammable substances. Therefore, when the unit is operated unattended in
an automated environment, an alarm function must be provided by the user to
indicate a malfunction in the associated equipment used.
Warning
Hg –LAMP CONTAINS MERCURY, Manage in Accord with Disposal Laws,
see: www.lamprecycle.org or 1-800-668-8752
Danger: Exposure to Mercury represents a health hazard to humans.
When unpacking or installing the lamp, always wear protective clothing and a face
mask. Operate the lamp inside the X-Cite®exacte housing only. This prevents
direct viewing of the arc and in the case of lamp bursting, contains the lamp
particles. In the rare instance in which a lamp bursting occurs, and the mercury
content is released, the following safety precautions are recommended; all
personnel should be immediately evacuated from the area to prevent inhalation of
the mercury vapour. The area should be well ventilated for a minimum of 30
minutes. Prior to clean up ensure an ‘approved-for-mercury’ respirator mask and
non-porous gloves such as latex or rubber are used. After the lamp housing
elements have cooled, the mercury residue should be collected with the use of a
special absorbing agent available from laboratory equipment suppliers.
Listed below are examples of internet web sites for obtaining Mercury Spill Kits;
http://www.rosshealthcare.org/Mercon.spill.kits.htm
http://www.coleparmer.ca/catalog/product_index.asp?cls=43577
http://www.environmental-
expert.com/technology.aspx?idCategory=2054&word=mercury%20spill%20kits
http://www.alibaba.com/products/spill_kit/4.html
Warning
Should this X-Cite®exacte unit be used in a manner not specified by Excelitas
Technologies, the protection provided by the equipment may be impaired.

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Warning
The method in which lamps are disposed of must comply with local rules and
regulations for disposal of hazardous materials. Lamps may be returned to
Excelitas Technologies providing they are returned in their original packaging.
Excelitas Technologies will dispose of them in the appropriate manner.
Warning
This unit is designed for bench top use only! Always ensure that the unit is
operated on a hard, stable surface. This will prevent obstruction of the bottom
chassis ventilation openings. Any obstruction of these openings could result in a
possible over-heating condition. Do not attempt to remove or tamper with the
rubber feet located on the bottom of the unit.
Caution
The lamp module’s operational life can be significantly shortened if it is handled
incorrectly. Do not touch the bulb’s glass envelope or the inside surface of the
reflector. Skin oils can cause the lamp module to fail prematurely.
Caution
Prior to opening the unit and handling the lamp module, allow a minimum of 5
minutes for the lamp module to cool down completely.
Caution
Any electronic equipment connected to the X-Cite®exacte must comply with the
requirements of EN/IEC 60950.
Cleaning:
Clean the exterior of the unit with a slightly dampened cloth and simple water/
detergent solution only.