AmeriWater Central Sterile RO2 Manual

3345 Stop 8 Rd, Dayton, OH 45414 | 800 535 5585 | www.ameriwater.com 98-0106 Rev K
Manufactured With Pride in the USA
Operation & Maintenance Manual
Central Sterile RO2
00HC-0045

TABLE OF CONTENTS HCRO2
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TABLE OF CONTENTS
TABLE OF CONTENTS ............................................................................................................................1
1USER INFORMATION......................................................................................................................3
1.1 Manual Definitions ............................................................................................................................3
1.2 Introduction........................................................................................................................................3
1.3 Electrical Leakage Standards ...........................................................................................................4
1.4 Cautionary Symbols ..........................................................................................................................4
1.5 Warranty Policy ..................................................................................................................................4
2TECHNICAL INFORMATION ...........................................................................................................5
2.1 System Specifications........................................................................................................................5
2.2 Environmental Conditions Anticipated...........................................................................................6
2.3 Transport Conditions Anticipated ...................................................................................................6
2.4 Theory of Operation..........................................................................................................................6
2.5 System Components .........................................................................................................................7
2.6 Electrical Schematic.........................................................................................................................10
2.7 Flow Schematic ................................................................................................................................11
3SYSTEM INSTALLATION ...............................................................................................................12
3.1 Pre-Installation Requirements .......................................................................................................12
3.2 General Installation .........................................................................................................................12
3.3 RO2 Installation................................................................................................................................12
4SYSTEM START-UP PROCEDURES ................................................................................................14
4.1 Caution..............................................................................................................................................14
4.2 Safety Features ................................................................................................................................14
4.3 RO ......................................................................................................................................................15
4.3.1 PT401 Priming Procedure...........................................................................................................17
4.4 System Shutdown............................................................................................................................19
5OPERATION AND MONITORING .................................................................................................20
5.1 Operation..........................................................................................................................................20
5.2 Bypass Procedure............................................................................................................................20
5.3 System Monitoring ..........................................................................................................................20
5.4 AAMI Monitoring..............................................................................................................................22
6DISINFECTION ..............................................................................................................................22
6.1 Disinfection Procedure ...................................................................................................................22
6.1.1 Disinfecting the RO2 ...................................................................................................................23

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6.2 A Word about Hydrogen Peroxide/Peroxyacetic Acid ................................................................25
7CLEAN IN PLACE (CIP) ..................................................................................................................27
8CONTROLLER................................................................................................................................29
8.1 RO Front Panel Controls and Indicators .......................................................................................29
8.2 Controller Operation.......................................................................................................................30
8.3 Controller Adjustments...................................................................................................................33
8.4 Standard Set-points.........................................................................................................................36
8.5 To Display or Change Set-points ...................................................................................................38
8.6 Changing Resistors On The RO Control Board For C2 Range ....................................................38
9MAINTENANCE.............................................................................................................................40
9.1 Maintaining the System ..................................................................................................................40
9.2 Pt401 Anti-Scalant............................................................................................................................40
9.3 RO Maintenance Instructions ........................................................................................................40
9.3.1 Membrane Replacement Procedure.........................................................................................40
9.3.2 Rinse Out Cycle ............................................................................................................................42
10 TROUBLESHOOTING AND REPAIR ..............................................................................................43
10.1 Troubleshooting Chart....................................................................................................................43
10.2 Controller Troubleshooting............................................................................................................45
10.3 Pump Repair.....................................................................................................................................47
10.4 Installing a Replacement Pump Assembly ...................................................................................47
10.5 Solenoid Test Procedure.................................................................................................................48
10.6 Solenoid Valve Replacement..........................................................................................................49
11 SPARE PARTS LISTING..................................................................................................................50
CALIFORNIA PROPOSITION 65...........................................................................................................52

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1USER INFORMATION
1.1 Manual Definitions
NOTE: This symbol points out important information for working with the system in a proper
manner.
WARNING: This symbol refers to a possible danger that threatens the safety and life of
persons.
CAUTION: This symbol refers to a possibly hazardous situation. Failure to observe these
references may result in minor injuries and/or damage to property.
1.2 Introduction
This system is designed to pretreat and purify water for use in sterile processing applications. The
system is shipped with required water treatment components. If the system will be running without
softened water, the PT401 system will need to be connected inside of the RO. This Operation
Manual was written specifically for the RO2 model. Your system was thoroughly tested and in
excellent condition when it was shipped to you. However, because damage during shipment is
possible, please unpack and carefully inspect the system as soon as you receive it. Please notify
AmeriWater if any problems are encountered.
Please read the Operation Manual before using the system. Contact AmeriWater Customer
Service with any questions at 1-800-535-5585 Monday through Friday 8:00 a.m. to 5:00 p.m. Eastern
Time. For after-hours emergencies call 1-800-535-5585 and follow the instructions on the recorded
message. Our on-call technician will return your call as soon as possible. This entire Operation
Manual should be read before operating or servicing the system. This Operation Manual should
then be kept near the system and used as a reference and troubleshooting guide.
WARNING: This Reverse Osmosis System (RO) contains a preservative solution to prevent
microbiological growth and freezing. Discard all product water for at least two hours of
operation before placing the RO in service.
Materials that Contact Product Water
ABS
Acrylic
Carbon
EPDM
Nylon
Polyester
Polyethylene
Polypropylene
PVC
Stainless Steel
TFCM* (Polyimide)
Tygon
*Thin Film Composite Membrane
All of the above listed materials meet FDA and/or NSF standards.
Safety Features: The RO is equipped with several safety features for the benefit of the user.
•INCOMING TAP WATER, PRODUCT WATER, and REJECT WATER TO DRAIN hoses are labeled to
prevent incorrect connections.
•An audible alarm sounds whenever water quality drops to an unacceptable level.

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1.3 Electrical Leakage Standards
The AmeriWater RO2 water treatment systems comply with the IEC 61010-1 Standards for Product
Safety and Construction.
The cabinet of the RO2 is PVC for additional operator safety.
The RO2 is compliant with IEC 61010-1 Safe Current Limits. All major components of the RO
(controller, pump, solenoid valve, antiscalant pump, etc.) are UL listed.
1.4 Cautionary Symbols
Caution, risk of electrical shock!
Attention, risque de choc électrique!
Open by qualified service personnel only!
Ouverture par le personnel qualifié seulement!
Refer to this Operation and Maintenance Manual for
instructions and safety considerations. Référez-vous au
manuel des Opérations et Entretien pour instructions
et mesures de sécurité.
Caution, risk of danger!
Attention, danger potentiel!
For service by qualified service personnel only!
Entretien par le personnel qualifié seulement!
Replace with 120Vac, 15amp, time-delay fuse only.
Remplacer avec 120Vac, 15 amp, fusible à
retardement seulement.
Earth Ground terminal
Borne de mise à la terre
This product has been tested to the requirements of
CAN/CSA-C22.2 No. 61010-1, second edition, including
Amendment 1, or a later version of the same standard
incorporating the same level of testing requirements.
Product marked with this symbol indicates that it is
protected throughout by double insulation or reinforced
insulation.
1.5 Warranty Policy
This product is covered under the standard AmeriWater warranty policy. For specific terms and
conditions, please contact your AmeriWater Sales Representative.
120
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2TECHNICAL INFORMATION
2.1 System Specifications
Ideal, minimum, and maximum
incoming water temperature
Min = 41° F (5° C)
Max = 90° F (33° C)
Ideal Temperature = 77° F (25° C)
Prefilter gauge pressure (when the
RO is running)
Minimum
Maximum
20 PSI
50 PSI (Pounds per Square Inch)
Pump pressure
Minimum
Maximum
100 PSI
180 PSI
Maximum output of product water
@ 77°F (25°C), TDS<1000 ppm of
NaCl, & pump pressure of 150 psi.
RO2 –APPROXIMATELY 2,800 GPD (APPROXIMATELY
10,598 LPD)
(Gallons Per Day / Liters Per Day)
Power Ratings
Voltage
Frequency
Phase Ø
Amp Draw
RO
120 VAC
60HZ
1Ø
14
RO Connection
Provided Hose Length
External Connection
Inlet Tap ½” Plain Hose
8 ft
¾”FGHT
Product ½” Plain Hose
4 ft
¾” MGHT
Reject to Drain ½” Plain Hose
8 ft
½” FPT
Electrical Requirements
Controller Power: 115V/60Hz/20A dedicated Dual Outlet GFI
(Ground Fault Interrupter) for RO controller
Shipping Weight
Operating Weight
507 lbs.
975 lbs.

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2.2 Environmental Conditions Anticipated
This device is intended to be used under the following conditions:
•Indoor use
•Altitude up to 6562 feet (2000 m)
•Temperature between 41°F and 104°F (5°C and 40°C)
•Maximum relative humidity 80% for temperatures up to 87.8°F (31°C) decreasing linearly to
50% relative humidity at 104°F (40°C)
•MAINS supply voltage fluctuations up to + 10% of the nominal voltage
•Transient overvoltages present on MAINS supply = CATEGORY II
•Applicable RATED POLLUTION degree 2
2.3 Transport Conditions Anticipated
•Altitude up to 6562 feet (2000 m)
•Temperature between 41°F and 104°F (5°C and 40°C)
•Maximum relative humidity 80% for temperatures up to 87.8°F (31°C) decreasing linearly to
50% relative humidity at 104°F (40°C)
2.4 Theory of Operation
The process of osmosis can be reversed by placing pressure upon the feed water side (concentrated
solution side) of the membrane. Water will be forced through the membrane barrier to yield water
that is purer on the lower pressure side of the membrane than on the more concentrated solution
side (higher pressure side) of the membrane. The feed water will become more “concentrated,” and
will be discharged through the reject port known as “reject water” or “concentrate”.
Hence, the liberation of purer water from its solutions is caused by the reversal of the osmotic
pressure; the operation is termed “Reverse Osmosis”. Reverse Osmosis is commonly referred to as
“RO”.
Plastic container filled with PT401 solution to prevent the RO membranes from scaling is provided
and should be used in the absence of a water softener.

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2.5 System Components
Figure 1: External Front View

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IDENTIFICATION OF COMPONENTS (Figure 1)
1. PRODUCT GPM: Flowmeter that measures the flow of the product water in gallons per minute
(GPM) and liters per minute (LPM).
2. PUMP PRESSURE: Gauge that measures the primary feed pressure in pounds per square inch
(PSI) from the pump through the RO2 membrane.
3. TEMPORARY FLUSH VALVE: When the unit is in operation, the valve must be in the
“OPERATION” position. Move the valve to the “FLUSH” position to bypass the flow control and
allow a faster flow of water to the drain during membrane flush.
4. CHLORINE SAMPLE PORT: Valve with nozzle to let small amounts of water out to test for the
presence of chlorines before the RO2 membrane.
5. PRODUCT WATER SAMPLE PORT: Valve with nozzle to let small amounts of water out to test
the quality of the product water.
6. PRODUCT WATER: Hose transmitting purified water from the RO2 system to the DI Polisher
or Storage Tank.
7. REJECT WATER TO DRAIN: Hose transmitting wastewater to the drain.
8. INCOMING TAP WATER: Hose feeding tap water into the RO2 system.
9. CONTROLLER: Control mechanism for the RO2.
10. SIDE ENTRY HOOD: External wire installation for float level switches.
11. PREFILTER INLET GAUGE: Gauge that measures the pressure in pounds per square inch (PSI)
of the incoming tap water as it enters the micron prefilter.
12. POST FILTER OUTLET GAUGE: Gauge that measures the pressure in pounds per square inch
(PSI) of the water after going through the carbon cartridge filter. Change the micron prefilter
when the outlet gauge reads 15 PSI less than the prefilter inlet gauge.
13. HOSPITAL GRADE POWER CORD: Must be connected to a single phase, 3-conductor type,
hospital grade receptacle with a ground fault interrupter (GFI) at 115V, 20amp, and 60Hz.
14. PAA QUICK CONNECT FITTING (RO): Quick connect fitting that the PAA container is
connected to on the RO2 for sanitization.

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Figure 2: Internal Rear View
IDENTIFICATION OF COMPONENTS (Figure 2)
15. CLEAN IN PLACE (CIP) SWITCH: When the CIP Switch is placed in the ON position, all RO2 fail-
safe modes are disabled for low pressure membrane cleaning with the optional AmeriWater
Clean In Place System (P/N 00CIP1).
16. CARBON CARTRIDGE FILTERS: Dual 10-micron carbon cartridges for removal of chlorine &
sediment.
17. PUMP: Provides the pressure for the RO2 system. The RO2 ON/OFF switch controls the pump
motor.
18. PT401 FEED PUMP: Injects PT401 Antiscalant / Scale inhibitor solution at a predetermined
dosage based on a water analysis. The PT401 pump runs when the main pump runs.
19. PT401 ANTISCALANT / SCALE INHIBITOR: Plastic container filled with 2 ½ gallons of PT401
solution to prevent the RO2 membrane from scaling. The plastic container should be refilled
with PT401 solution at least monthly or when on when it reaches the half-full level.
20. MEMBRANES: Spiral-wound, thin film, composite membranes for reverse osmosis.

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2.6 Electrical Schematic
Figure 3: Electrical Diagram, RO2

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2.7 Flow Schematic
Figure 4: RO2 Flow Schematic

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3SYSTEM INSTALLATION
3.1 Pre-Installation Requirements
1. Utility requirements;
a. Plumbing: ¾” ball valve with NPT threads and dynamic pressure of at least 20 PSI at
10 GPM.
b. Electrical: see Section 2.1.
2. Hot and cold city/tap water supply line flushed and free of debris before equipment
installation.
3. Floor drain within 4 feet of the RO/DI system capable of handling up to 10 GPM flow.
4. Supply piping to the washer/disinfector/sterilizer system(s) within 3 feet of the RO/DI
distribution pump with a shut off valve.
5. Access space of 1 foot on each side and behind the water treatment equipment with a 3 foot
aisle in front of the equipment or required to meet local codes.
3.2 General Installation
1. Two (2) hose clamps are to be used per hose connection.
2. A visual inspection is to be performed upon finishing installation and start-up of each system
to ensure each component will function as intended. This includes, but is not limited to: lids
and respective O-rings are not damaged or loose, damaged or loose hoses, leakage, secured
power outlets, etc.
3.3 RO2 Installation
1. The electrical source must be single phase, 3-conductor type provided with a hospital grade
receptacle and a ground fault interrupter (GFI) at 115V, 20amp, and 60Hz. The proper
polarity and ground integrity must be initially checked and thereafter maintained. Failure to
do so may result in electrical shock to the operator. It is suggested that the RO be placed on
an electrical supply with emergency backup.
2. The RO must only be plugged directly into a GFI receptacle. It must not be plugged into an
extension cord or power strip that could cause low amperage.
CAUTION: To avoid electrical shock, always unplug the RO system before opening the face of
the electrical controller.
3. Incoming water should be between 41°F and 90°F (5°C and 33°C). It is not recommended to
use water at temperatures below 41°F (5°C) as it will reduce membrane performance
significantly. Use only the cold water supply unless using an automatic blending valve to get
77°F (25°C) water. Never use water warmer than 90°F (33°C).
4. Water with silt density index (SDI) above 5 SDI will foul the membrane.
5. The RO system may be equipped with a pretreatment system to remove chlorine. It is
important to test for chlorine at the chlorine sample port periodically. Chlorine will

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deteriorate the membrane and cause system failure. It is recommended to use a chlorine
test strip.
6. Incoming tap water pH should be within EPA National Secondary Drinking Water Regulations
of 6.5 –8.5. Incoming tap water with pH higher or lower than the regulation will cause higher
conductivity in the product water. If the water changes drastically, the membrane will be
harder to clean. Periodically check the pH of the incoming tap water to verify that it is within
the specified range (IBT pH Water Test Strips P/N 97PH20901).
7. Always maintain water flow and pressure to avoid damage to the pump.
8. Minimum feed pressure is 20 PSI @ 6 GPM (while the RO is in operation, with flow).
Maximum feed pressure is 50 PSI.
9. Remove the plugs from the product water, reject water to drain, and incoming tap water
fittings on the front of the RO.
10. Connect the INCOMING TAP WATER,
PRODUCT WATER and REJECT WATER TO
DRAIN hoses to the appropriate fittings
on the RO. All hoses should run to the
right side of the RO. This will allow you to
move the RO forward to access the rear
panel.
NOTE: To remove the hoses, depress the gray
“collet” inward while gently pulling the hose out.
The tubing extension MUST be fully inserted into
the fitting body to the tube stop.
CAUTION: To ensure proper assembly, tubing
extension MUST be fully inserted into the
fitting body to the tube stop.
11. Open the back cover and remove any packing foam.

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4SYSTEM START-UP PROCEDURES
4.1 Caution
NOTE: This entire Operations Manual should be read before operating or servicing the RO system.
The Operations Manual should then be kept near the system and used as a reference and
troubleshooting guide.
WARNING: This Reverse Osmosis System (RO) contains a preservative solution to prevent
microbiological growth and freezing. Discard all product water for at least two hours of
operation before placing the RO in service.
1. It is important to test for chlorine and chloramines at the CHLORINE SAMPLE PORT before
each use of the system. Chlorine will deteriorate the membrane and cause system failure. It
is recommended to use a Total Chlorine test kit, such as Water Check 2 Low Level
Chlorine/Chloramines Test Strips (P/N 97CM20201).
CAUTION: Mixing chlorine and hydrogen peroxide/peroxyacetic acid causes a toxic chemical
reaction. Never allow them to mix! Do not use chlorine to disinfect the system!
2. Use only the exact amount of hydrogen peroxide/peroxyacetic acid disinfectant solution
(PAA) and in proper dilution during disinfection of the system.
3. It is important to test for PAA in the Product Water after rinsing during disinfection of the
system. Do not use the system until all traces of the disinfecting solution in the Product
Water are gone.
WARNING: The Clean In Place Switch, located inside the cabinet on the back of the controller,
must be in the OFF position during normal operation. If the Clean In Place Switch is left in the
ON position during normal operation, all RO fail-safe modes will be disabled, and damage to
the RO.
4. Ameriwater recommends installing a sediment filter added as pretreatment for the RO.
4.2 Safety Features
The RO is equipped with several safety features for the benefit of the user. They consist of the
following:
Disinfection using (PAA) disinfecting solution instead of formaldehyde increases safety and avoids
health risks associated with formaldehyde. Using PAA does not require additional ventilation, and
disposal is safe and easy. Important information regarding the usage and handling of PAA is listed in
Section 6.2, A WORD ABOUT HYDROGEN PEROXIDE/PEROXYACETIC ACID. Please read carefully.
INCOMING TAP WATER, PRODUCT WATER, and REJECT WATER TO DRAIN hoses are labeled to
prevent incorrect connections.
Low pressure shutdown protects the pump if the feed pressure drops below 20 PSI.

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An audible alarm sounds whenever water quality drops to an unacceptable level.
4.3 RO
1. Lock the two front casters so that the RO will remain stationary during start-up.
NOTE: The incoming, product, and reject hoses were installed on the RO in the install steps.
NOTE: Before connecting incoming feed water to RO, ensure feed water line is routed to drain
and until the water is clear to prevent rusty water from clogging the system.
2. Connect the RO incoming tap water hose to the potable cold water supply using the
incoming tap water hose and fittings supplied. If utilizing a blending valve, ensure hot and
cold water lines are flushed prior to operating the RO. Adjust blend valve to 77°F (25°C). Do
not exceed 82°F (28°C).
3. The reject water to drain hose coming out of the RO system is for reject water. The water
from this hose will go down the sink or drain. Leave at least a 2" air gap between the hose
and the drain to prevent contamination or siphoning.
4. The product water hose should also be secured to the drain until the start-up flush and
initial disinfection cycle are completed, and the water quality is in the good range (below the
conductivity set-point, and not in alarm).

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5. Open the access cover and make sure that the “CIP” switch is in the “ON” position.
6. Plug the controller power cord into a dedicated 115-volt, 20-amp GFI receptacle.
WARNING: Before start-up, membranes on the RO will need to be wetted in order to prevent
damage caused by air pressure.
7. Turn on the potable water supply to the RO.
8. Inorder to allow the membranes to fill with water, press the power button and allow the 10
second pump timer to count down to 2 seconds before turning it off. This will allow water to
be pushed through the system at a low pressure.
9. Repeat previous step up to 10 times. This may take up to 5 minutes. Ensure water is flowing
through the drain and flowmeters.
10. Open the access cover and switch the “CIP switch to the “OFF” position.
11. Turn on the RO by pressing the POWER key, and allow it to run making sure the water is
properly flowing out the Reject and Product hoses.
12. With the RO operating, turn the FLUSH VALVE to the OPERATION position.
13. The user can verify that the RO is operating correctly by checking the flowmeters, the
controller, and the inlet and outlet pressure gauges. The flowmeters will show movement on
the flow bobbers in the flowmeters. The controller will show on the screen operating
parameters, such as quality of water and temperature of water. The incoming and outlet
gauges will show pressure reading on the gauge. There will be a differential of pressure
between the two gauges (outlet subtracted by the inlet).
14. At this point, the preservative in the RO and the membranes needs to be completely flushed
from the RO.
15. Turn the FLUSH VALVE to the FLUSH position to allow the RO to run in full-flow reject flush
for about fifteen minutes. Afterwards, turn the FLUSH VALVE to the OPERATION position and
run for an additional 2 hours. Check for leaks during this time.
NOTE: The RO conductivity alarm may sound, which is normal when it is in FLUSH. Press the ALARM
SILENCE key on the RO controller to silence the alarm. The alarm will restart after a 3-minute delay.
16. The conductivity value, after flushing and being put back into the service mode, must be
within the acceptable limit.
17. After a thorough flushing of the preservative, the RO must be disinfected prior to being put
into service for use. Reference section 6.1.1 for the disinfection procedure.

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18. When all disinfection procedures have been completed, turn on the feed water supply.
19. Press the power key (the display will show operating after a ten second delay).
NOTE: The conductivity may alarm for a few seconds before dropping into the desired range.
20. Press the power key (the display will show standby). Connect the product water hose to the
direct feed loop or storage tank inlet (be sure to connect the product water hose aseptically).
21. Refer to the Monitoring Log in Section 5.3. Complete the log, making sure that the system is
operating within all the required ranges.
WARNING: Do not use the RO until all specifications are met.
CAUTION: Although the water treatment system may produce water of sufficient quality to meet the
requirements of AAMI standards, distribution of the water may degrade its quality to the point
where it no longer meets the requirements of this standard. AmeriWater offers information about
ultra-pure water piping to prevent the degradation of product water in a water loop.
4.3.1 PT401 Priming Procedure
WARNING: Do not use the PT401 anti-scalant if your system has softened water. Turn off the
system by depressing the on / off button on the PT401 pump.
AmeriWater RO products featuring the PT401 anti-scalant system are equipped with a priming valve.
The following procedure should be followed during the initial start-up of the RO system, and
whenever the PT401 pump loses its prime:
1. Open the priming valve by turning it to counterclockwise three times.
2. Verify that the RO is on and running.
3. Be sure the injection pump’s suction line is immersed in solution in the PT401 container.
4. Press the ON key to turn on the PT401 injection pump.

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5. Press the “DOWN” key to cycle the STROKE RATE to
approximately 360.
6. Run the injection pump until a stream of PT401
without air bubbles flows out of the air bleed tube.
7. Close the adjustment knob completely by turning
clockwise until the knob stops, making sure there is
no liquid flow out of the air bleed tubing.
If the pump does not prime, repeat the foregoing steps.
Once the pump has been primed, and is pumping the
chemical through the head into the water stream, adjust the
stroke rate to the recommended settings below.
HARDNESS (Grains Per Gallon)
STROKE SETTINGS
1-6 GPG
4
7-20 GPG
5
21-40 GPG
6
For water with hardness greater than 40 GPG, contact AmeriWater for consultation.
At the stroke rate of ten, the PT401 injection pump will put about 30 milliliters (approximately 10
liquid ounces) per hour into the RO incoming water stream.
Repeat these steps as necessary when the system is started after sitting for extended periods or the
PT401 Bottle is empty and air has drawn into the pump. The priming may not take as long as the
initial time.
PT401 Anti-scalant/Scale Inhibitor
Contaminants
Chemical Feed System
Membrane Scale Control
Not to exceed 40 ppm
WARNING: Overdosing PT401 will adversely affect (blind) the membrane causing a decrease
and/or loss of permeate flow.
WARNING: If softened water is supplied, turn off the PT401 system by depressing the on/off
button on the chemical feed pump and emptying the PT401 jug.