Amersham Pharmacia Biotech AKTA design pH/C-900 User manual

User Manual
18-1120-06
Monitor pH/C-900
18-1120-06_AC.qxd 1999-04-14 14:16 Sida 1 (1,1)

Trademarks
ÄKTA and UNICORN are trademarks of Amersham
Pharmacia Biotech Limited or its subsidiaries.
Amersham is a trademark of Nycomed Amersham plc.
Pharmacia and Drop Design are trademarks of
Pharmacia & Upjohn Inc.
Terms and Conditions of Sale
All goods and services are sold subject to the terms
and conditions of sale of the company within the
Amersham Pharmacia Biotech group which supplies
them. A copy of these terms and conditions of sale is
available on request.
Addresses
Amersham Pharmacia Biotech AB
SE-751 84 Uppsala
Sweden
Amersham Pharmacia Biotech UK Limited
Amersham Place
Little Chalfont
Bucks, HP7 9NA
England
Amersham Pharmacia Biotech Inc
800 Centennial Avenue
PO Box 1327
Piscataway, NJ 08855
USA
Amersham Pharmacia Biotech Europe GmbH
Postfach 5480
D-79021 Freiburg
Germany
©Amersham Pharmacia Biotech AB 1999
– All rights reserved
Important user information
Meaning: Consult the instruction manual to avoid
personal injury or damage to the product or other
equipment.
WARNING!
The Warning sign is used to call attention to the
necessity to follow an instruction in detail to avoid
personal injury. Be sure not to proceed until the
instructions are clearly understood and all stated
conditions are met.
CAUTION!
The Caution sign is used to call attention to
instructions or conditions that shall be followed to
avoid damage to the product or other equipment. Be
sure not to proceed until the instructions are clearly
understood and all stated conditions are met.
Note
The Note sign is used to indicate information
important for trouble-free or optimal use of the
product.
Should you have any comments on this instruction, we
will be pleased to receive them at:
Amersham Pharmacia Biotech AB
SE–751 84 Uppsala
Sweden
!
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Contents
1 Introduction
1.1 General ..................................................................................................................1
1.2 Safety.....................................................................................................................1
2 Installation
2.1 Unpacking..............................................................................................................2
2.2 General precautions ...............................................................................................2
2.3 Installing the conductivity cell ...............................................................................2
2.4 Installing the pH electrode.....................................................................................3
2.5 Connecting electrical signal cables .........................................................................4
2.6 Connecting to communication link........................................................................4
2.7 Connecting to supply voltage.................................................................................5
2.8 Preparing the instrument for use............................................................................5
3 Operation
3.1 On/off....................................................................................................................6
3.2 Menu selection and settings ...................................................................................6
3.3 Main menu overview .............................................................................................7
3.4 Reading pH and conductivity values......................................................................8
3.5 Setting conductivity scale.......................................................................................8
3.6 Calibrating pH.......................................................................................................9
3.7 Calibrating conductivity ......................................................................................11
3.8 Using an external chart recorder..........................................................................11
3.9 Storage and shut-down ........................................................................................12
3.10 Restart after power failure...................................................................................12
4 Maintenance
4.1 Periodic maintenance ...........................................................................................13
4.2 Cleaning-in-place .................................................................................................13
4.3 Cleaning and checking the pH electrode..............................................................13
4.4 Changing pH electrode ........................................................................................14
4.5 Cleaning the conductivity flow cell......................................................................14
4.6 Changing conductivity flow cells .........................................................................14
4.7 Instrument housing ..............................................................................................14
5 Trouble shooting
5.1 General ................................................................................................................15
5.2 Faults and actions................................................................................................15
5.3 Error messages.....................................................................................................18
Monitor pH/C-900
User Manual 18-1120-06 Edition AC i
Contents
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Reference information
A Description ..........................................................................................................19
A.1 Instrument.................................................................................................19
A.2 pH electrode..............................................................................................20
A.3 Conductivity cell .......................................................................................20
B Menus..................................................................................................................21
B.1 Check menu ..............................................................................................21
B.1.1 Check Monitor Cell and Electrode ...........................................21
B.1.2 Check Service Mode .................................................................21
B.2 Setup menu ...............................................................................................21
B.2.1 Setting up conductivity temperature compensation...................21
B.2.2 Calibrating the conductivity cell ...............................................22
B.2.3 Entering the conductivity cell constant .....................................23
B.2.4 Setting up pH temperature compensation.................................24
B.2.5 Setting up the pH display..........................................................24
B.2.6 Calibrating the temperature sensor...........................................24
B.2.7 Setting up the temperature display............................................25
B.2.8 Selecting language.....................................................................25
B.2.9 Setup unit number ....................................................................25
B.2.10 Setup display angle ...................................................................25
B.3 Setting and using the alarm timer..............................................................26
B.4 Service displays .........................................................................................26
B.5 Menu overview .........................................................................................27
C Technical specifications .......................................................................................28
D Accessories and spare parts..................................................................................31
Short instructions on back page
About this manual
This manual comprises two parts; a practical part (sections 1 – 5)
and a reference part (sections A – D).
Sections 1 – 5 contain the necessary information for operating the
instrument.
Monitor pH/C-900
User Manual 18-1120-06 Edition AC
ii
Contents
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1 Introduction
1.1 General
Monitor pH/C-900 is a high precision on-line monitor for
measurement of pH and conductivity in liquid chromatography.
The pH/C-900 features:
• Fast response
• High accuracy and reproducibility
• Flow cells with low dead volume
• Accurate and reliable monitoring through self-test and
self–calibration
• Flow cells can be connected close together, minimizing band
broadening and time delay between detectors.
1.2 Safety
• The instrument is designed for indoor use only.
• Do not use in a dusty atmosphere or close to spraying water.
• Operate in accordance with local safety instructions.
pH/C-900
Monitor pH/C-900
User Manual 18-1120-06 Edition AC 1
WARNING! The instrument must not be opened by the user. It
contains high voltage circuits which can be capable of delivering a
lethal electric shock.
WARNING! Always disconnect the power supply before
attempting to replace any item on the instrument during
maintenance.
WARNING! The instrument must be connected to a grounded
mains socket.
Introduction 1
WARNING! When using hazardous chemicals, all suitable
protective measures, such as protective glasses, must be taken.
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2 Installation
2.1 Unpacking
Unpack the instrument and check the items against the supplied
packing list. Inspect the items for obvious damage which may have
occurred during transportation.
It is recommended that all packing materials should be retained if
onward transport of the instrument is expected.
2.2 General precautions
The instrument should be located in a place of low temperature
variations, away from heat sources, draughts and direct sunlight.
The instrument may be operated at normal ambient temperatures in
the range +4 to 40 °C.
The instrument should be installed on a stable laboratory bench or
in ÄKTA™ explorer or ÄKTA™ purifier. To ensure correct
ventilation a free space of 0.1 m is required behind and in front of
the instrument. Do not use any soft material under the instrument,
to ensure that the ventilation inlet in the front is not blocked.
2.3 Installing the conductivity cell
1 Place the conductivity cell in a suitable
place, for example on the shelf of the
Monitor UV-900 using the clip provided
with the UV monitor. The cell can be
placed up to 1.5 m from the monitor
housing.
2 Connect the conductivity cell to the socket
Conductivity Flow Cell on the rear panel of
the instrument.
3 Connect the tubing with the “Fingertight” connectors. The flow
cell itself does not have a recommended flow direction. In
conjunction with the pH electrode, place the conductivity flow
cell and select its flow direction so that the screw head end of the
flow cell faces the pH flow cell (in ÄKTApurifier systems, the pH
electrode is optional).
CAUTION! The following information should be read carefully
to ensure that the instrument is installed correctly.
Monitor pH/C-900
User Manual 18-1120-06 Edition AC
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2 Installation
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2.4 Installing the pH electrode (optional in ÄKTApurifier systems)
Mounting the flow cell holder
1 Hook the flow cell holder on the right hand
side of the housing. Secure it with the slide
clamp.
If the flow cell holder is not used, the flow
cell must still be installed at an angle of 30°
from the vertical with the outlet placed
higher than the inlet to prevent air bubbles
being trapped in the cell.
The flow direction is marked on the flow cell.
2 Connect the tubing with the “Fingertight” connectors.
Inserting the pH electrode
Note: Handle the pH electrode with care.
1 Unpack the pH electrode. Ensure that it is not broken or dry.
2 Prior to first using the electrode, remove the electrode end cover
and immerse the glass bulb in buffer for 30 minutes.
3 Remove the dummy electrode from the
flow cell and store it in the flow cell
holder.
4 Carefully insert the electrode in the
flow cell. Tighten the nut by hand to
secure the electrode.
Note: If the electrode is not fully
inserted, the system will leak and a
dead volume will occur in the holder.
5 Connect the pH electrode cable to the
rear of the instrument to the socket pH
Probe.
CAUTION! The tip of the pH electrode consists of a thin glass
membrane. Protect it from breakage, contamination and drying
out or the electrode will be destroyed. Always store the electrode
with the end cover filled with a 1:1 mixture of pH 4 buffer and
1 M KNO3. Do NOT store in water only.
Monitor pH/C-900
User Manual 18-1120-06 Edition AC 3
Installation 2
End
cover
To instrument
rear
”pH Probe”
Electrode
Flow direction
Dummy
Flow cell
holder Flow cell
Nut
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2.5 Connecting electrical signal cables
The sockets for electrical signals are located on the rear panel.
Connecting to chart recorder (if used)
The external chart recorder outputs for pH and conductivity from
the monitor are 0 – 1 V. There is also a 4 – 20 mA signal available
at the same output connectors.
1 Connect the chart recorder to the DIN-socket pH or Cond using
the cable supplied:
a. For 0 - 1 V use wire 1 (+) and 2 (–). Use a recorder with
floating inputs (i.e. none of the inputs connected to ground).
b. For 4 - 20 mA use wire 5 (+) and wire 6 (–).
Note: The signal cable is delivered with protective covers on each
wire. Do not remove the protective covers from unused
connections as a short circuit may disturb the measurements.
2 Set the recorder to 0–1 V or 4 - 20 mA input, full scale.
2.6 Connecting to communication link
The monitor can be used in ÄKTAexplorer and ÄKTApurifier, and
can be controlled from a PC running UNICORN™ version 2.20 or
higher, using UniNet cables.
Connect two UniNet cables to the UniNet 1 connectors. The
instrument can be connected in series anywhere between the PC and
a termination plug. The UniNet 1 link connects, in series, the PC
with Pump P-900, Monitor pH/C-900, Monitor UV-900 and the
Frac-900. The termination plug is connected to the last instrument in
the chain.
Analogue out 0-1V
UniNet 1 Mains On/off
pH Cond
Conductivity
Flow Cell
pH
Probe
pH
Ground
Monitor pH/C-900
User Manual 18-1120-06 Edition AC
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2 Installation
CAUTION! The mains power to ÄKTApurifier must be switched
OFF before connecting the instrument to the UniNet 1 link.
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2.7 Connecting to supply voltage
1 Make sure the on/off switch is in the OFF-position (O).
2 Connect the supplied mains cable between the instrument and a
grounded mains socket. Any voltage 100–240 V AC, 50–60 Hz
can be used.
The instrument contains no user replaceable fuse.
2.8 Preparing the instrument for use
Before the instrument is ready to use:
• Set the conductivity cell constant, see section B.2.3.
• Calibrate the temperature sensor, see section B.2.6.
• Calibrate the pH electrode (optional in ÄKTApurifier), see
section 3.6.
Note: The conductivity cell constant is shown on the packaging.
Retain the packaging in case the conductivity cell constant needs to
be re-entered.
Note: The measured temperature is the temperature in the
conductivity flow cell, which can differ from the ambient
temperature, in ÄKTAexplorer systems.
Note: When running chromatography using organic solvents, it is
recommended that the pH electrode is removed and the dummy
electrode inserted in its place, as organic solvents will cause pH
electrode degeneration.
Before performing these procedures you are recommended to read
sections 3.1–3.3.
Monitor pH/C-900
User Manual 18-1120-06 Edition AC 5
Installation 2
WARNING! The instrument must be connected to a grounded
mains socket.
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Monitor pH/C-900
User Manual 18-1120-06 Edition AC
6
3 Operation
3 Operation
3.1 On/off
Switch on the instrument at the mains switch on the rear panel.
At switch on, the instrument performs a selftest and then starts
calibration. After approx. 30 seconds the display shows
Cond Temp pH and the instrument is ready to use. All parameters
are factory set to default values.
If the conductivity cell is not connected the Temp and Cond fields are
blank. The pH field is blank if Show pH is set to off.
The monitor can be used immediately but the full specifications are
not obtained until after a 1 hour warm–up.
3.2 Menu selection and settings
Moving between menus
A specific menu is selected by turning the front selection dial
clockwise or counterclockwise. When the required menu is visible
the menu or selection is accepted by pressing the OK-button.
Menu selection OK-button ESC-button
Select sub-menu Return one menu level
If a menu has sub levels, the sub menu is displayed by pressing the
OK-button. Pressing the ESC-button moves back one menu level.
Calibrating
Please wait...
Selftest
Please wait...
Cond Temp pH
25.4% 22.9°C 11.50
Main menu 1
Main menu 2
Main menu 3
Main menu 4
Main menu 5
Main menus Sub menus Sub menus
Sub menu 2.3.1
Sub menu 2.3.2
Sub menu 2.1
Sub menu 2.2
Sub menu 2.3
Sub menu 2 .1.1
ESC
OK
ESC
OK
ESC
OK
Monitor pH/C900
V1.00
Calibration OK
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