Analytik Jena UVP iBox Explorer2 Manual

iBox®Explorer Imaging Microscope
Instruction Guide
______________________________________________________________________________
UVP, LLC
2066 W. 11th Street, Upland, CA 91786
Tel: [phone]
Fax: [phone]
Web Site: www.uvp.com 81-0342-01 Rev E
Ultra-Violet Products Ltd.
Unit 1, Trinity Hall Farm Estate
Nuffield Road, Cambridge CB4 1TG UK
Tel: [phone] Fax: [phone]
2
™

iBox Explorer22
Table of Contents
Table of Contents.........................................................................................................................................................2
Introduction..................................................................................................................................................................3
Safety Information........................................................................................................................................................4
Components.................................................................................................................................................................5
Specifications............................................................................................................................................................6
Darkroom Cabinet.....................................................................................................................................................6
Four Position Filter Tray............................................................................................................................................6
CCD Camera and Objectives....................................................................................................................................6
Magnification and Field of View.................................................................................................................................6
Viewer Window..........................................................................................................................................................6
Motorized Sample Platform.......................................................................................................................................6
Warming Plate...........................................................................................................................................................6
VisionWorksLS Software...........................................................................................................................................6
BioLite Xe MultiSpectral Source................................................................................................................................6
Installation....................................................................................................................................................................7
Installing the Software...............................................................................................................................................7
Registering the Software...........................................................................................................................................7
Installing the Hardware..............................................................................................................................................9
BioLite Xe MultiSpectral Light Source Setup...........................................................................................................10
Fiber Optic Cable Connection .................................................................................................................................11
Platform Stage/Warming Plate................................................................................................................................12
Additional iBox Explorer2Connections....................................................................................................................13
Loading Drivers.......................................................................................................................................................13
Operation....................................................................................................................................................................14
Preparing the System..............................................................................................................................................14
Preparing to Capture Images..................................................................................................................................15
Capturing Images....................................................................................................................................................18
Service Procedures....................................................................................................................................................19
Care and Cleaning ..................................................................................................................................................19
Return Procedure....................................................................................................................................................19
Troubleshooting.......................................................................................................................................................19
Replacement Parts and Accessories.......................................................................................................................20
Technical Support ...................................................................................................................................................21

iBox Explorer23
Introduction
The iBox Explorer2Imaging Microscope is designed to automate research with one-touch preset or user-defined PC
controls for accurate, repeatable imaging and analysis of animals and plants. The iBox Explorer2incorporates a light
tight darkroom with VisionWorksLS Acquisition & Analysis software for automated control. The system provides a
wide range of magnification settings, allowing visualization from an entire small animal down to the cellular level
subcutaneously and within the body cavity of living mice.
The darkroom has a door-mounted viewing window, four-position emission filter tray with four emission filters
included, and a software- and joystick-controlled imaging platform. The system comes with dual-path directed lighting
for exciting samples with the BioLite™ Xe MultiSpectral Light Source. The BioLite Xe offers an eight-position filter
wheel, six-position intensity selector, and a direct lighting source using fiber optic bundles to tightly control the output
spectrum for consistent, repeatable measurements. Two excitation filters are included, one each for the excitation of
GFP (Green Fluorescent Protein) and RFP (Red Fluorescent Protein).
The iBox Explorer2Imaging Microscope comes equipped with a slide-out warming plate that maintains the platform
stage surface at a constant 38°C. The warming plate also contains two threaded studs to accommodate a small
animal anesthesia manifold.
The iBox Explorer2includes a highly sensitive CCD camera with high magnification optics, providing real-time, live
preview images.
iBox Explorer2Imaging Microscope

iBox Explorer24
Safety Information
Do not use this equipment in a manner other than as stated in these instructions.
This equipment is designed for use in a laboratory environment by personnel knowledgeable in safe laboratory
practices. Proper precautions must be taken as potential electrical and mechanical hazards are inherent in the
use of this equipment.
CAUTION: While UVP’s warming plate is designed for the safety of users, care should be taken when operating
the unit as the platform stage can be hot.

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Components
The iBox Explorer2Imaging Microscope is comprised of the following components:
Darkroom Cabinet
Joystick
CCD Camera/Optics (internal and not visible)
Sample Viewer Window and Internal Amber Screen
Motorized Platform
Slide-Out Warming Plate
Emission Filters (GFP, RFP, 515 Long Pass, Neutral Density)
Excitation Filters (GFP, RFP)
BioLite Xe MultiSpectral Light Source
VisionWorksLS Software
Please review the packing slip for a complete equipment list. System components may vary.
Note: The system may also include a computer and monitor.
Monochrome CCD camera and
magnification objectives are
housed inside the darkroom
Four position filter tray with
RFP, GFP, 515 Long Pass and
Neutral Density filters included
Access ports for
BioLite Xe
MultiSpectral Light
Source
Viewer window opens for
sample observation through
amber screen
Light tight darkroom with
wide access door
Software controlled
platform adjusts on the
X, Y and Z axes
Warming plate
VisionWorks®LS software
controls darkroom functions,
image capture & image analysis
BioLite Xe
with indicator
lights

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Specifications
Power Requirements: 100-230V; 6.3 Amps at 120 Volts
Mains supply voltage fluctuations are not to exceed 10 percent of the nominal supply
voltage
Pollution Degree: 2
Installation Category: II
Altitude: Up to 2000m
Ambient Temperature: 5°C to 40°C
Software Requirements: Windows XP (SP2) or Windows 7 (32-bit and 64-bit versions)
Humidity: Maximum relative humidity of 80% for temperatures up to 31°C, decreasing linearly
to 50% maximum relative humidity at 40°C
Darkroom Cabinet
The iBox Explorer2is constructed of aluminum and fabricated to provide a light tight chamber. Darkroom
dimensions are 17.5”W x 20”D x 42”H (44.5cm x 50.8cm x 106.7cm).
Four Position Filter Tray
The filter tray accommodates up to four removable emission filters. The iBox Explorer2 system includes RFP
(Red Fluorescent Protein), GFP (Green Fluorescent Protein), 515 Long Pass and Neutral Density emission
filters. VisionWorksLS software controls the selection of filters. Additional filters are available through UVP.
CCD Camera and Objectives
The high-sensitivity CCD camera and fast optics, housed in the top of darkroom, generate high resolution
images. The camera and motorized optics are controlled by VisionWorksLS software.
Magnification and Field of View
The iBox Explorer2contains a combination of relay and objective lenses, providing magnifications of 0.17x,
0.25x, 0.50x, 1.66x, 2.50x, 4.50x, 7.50x, 8.80x and 16.5x. The corresponding fields of view are 90mm2, 60mm2,
30mm2, 9mm2, 6mm2, 3.3mm2, 2mm2, 1.7mm2and 0.9mm2.
Viewer Window
The viewer window, built into the darkroom door, opens for sample viewing.
Motorized Sample Platform
The motorized sample platform, controlled by the joystick, can be adjusted in the X, Y and Z dimensions. The
platform can be moved 100mm in each direction.
Warming Plate
The warming plate creates a constant temperature of 38ºC for the warming of animal specimens. The dark
background creates an ideal imaging surface while maintaining a constant temperature for the animal.
VisionWorksLS Software
The VisionWorksLS Acquisition & Analysis software controls the darkroom functions and lighting as well as the
motorized optics and camera. The software is operational on both 32-bit and 64-bit Windows operating systems.
BioLite Xe MultiSpectral Source
The BioLite Xe MultiSpectral Source provides dual path excitation lighting for specimens. GFP and RFP filters
come standard with the BioLite Xe, and custom filters for specific applications are also available. Refer to
“Replacement Parts and Accessories” for ordering information.

iBox Explorer27
Installation
Installing the Software
Insert VisionWorksLS CD (not network CD) into the computer.
Click on the Install button for VisionWorksLS.
Click OK, Next, agree to “I accept terms of licensing agreement”, then Next. Leave all options in
their default settings. Then click Next, Next, Install and finally Finish.
Registering the Software
Open the Software

Double click VisionWorksLS software icon
on the desktop.
To activate the software, registration is
required. To immediately activate the
software online, choose On-the-Fly
activation. If the computer is not
connected to the Internet, select Offline
activation and proceed to the following
page of this manual, or call UVP to register
the software.
Click Next to continue.
The Already have an activation ID option
is useful when reloading the software after
receiving an initial activation code.

Complete all required information on the
form.
Fill out the Serial Number located on the
CD. The number should be four sets of six
numbers.
Once the form is completed, click on Get
Activation No. and then click Activate
once the Activation Number appears in the
box.

iBox Explorer28

If the computer is not connected to the
Internet, click Offline activation to register
the software. This allows the user to obtain
the activation code and enter it at another
time.
Click Next to continue.

Click the link provided and complete the
form to obtain instructions. Click Finish.

iBox Explorer29
Installing the Hardware
Darkroom Setup
When unpacking and setting up the darkroom, two people are required to move the darkroom.
Place the darkroom on a flat surface which can provide adequate support for up to 120 pounds.
NOTE: This system is designed for indoor use only.
Darkroom Filter Setup
Place the filters into the filter tray in the appropriate positions as noted below. Once all filters are in
place, position the magnetic filter holder over the filter tray to hold the filters in place.
Open the darkroom door
and slide the filter tray
well door open. Place the
filter tray into position with
the lip facing up (note that
the filter must be set to
position #2 in
VisionWorksLS in order
for the filter tray to be
inserted or removed).
Filter Position #1 – 515 Long Pass
Filter Position #2 – 535/45 GFP
Filter Position #4 – Neutral Density
Filter Position #3 – 605/50 RFP
Filter Tray
Filter Holder
Lip facing up
Filter tray well door

iBox Explorer210
Dichroic Splitter Cube Installation

The dichroic splitter cube is used
to direct coaxial lighting down
through the magnification
objectives while allowing sample
emission lighting to pass through
to the camera.
Slide the dichroic splitter cube door
open to the left (the door is
embossed with the UVP logo) and
fully insert the dichroic splitter
cube. Fully slide the door to the
right once the cube is in place.
Camera Assembly

The system will come shipped with the camera and objectives installed from the factory. No
additional assembly is required.
BioLite Xe MultiSpectral Light Source Setup
Note: Image shown may appear different from the product
shipped.
Position the BioLite Xe to the left of the darkroom,
turn off the unit and unplug the power cord.
The xenon bulb assembly will come separate from the
BioLite Xe and is equipped with a male connector on
one end and a female connector on the other.
CAUTION: Wear safety glasses when handling the
xenon bulb assembly!The bulb is under high
pressure and can explode if dropped or mishandled.
To insert the bulb, lay the BioLite Xe on its back so
that the bottom of the unit is exposed. Connect the
male connector from the bulb to the female connector
in the BioLite Xe and vice versa.
CAUTION: Do not touch the glass portion of the
xenon bulb assembly! Residual fingerprint oils can
cause the bulb to explode during use.
Connect the bulb base plate to the bottom of the unit
using the three supplied nuts.
Return the BioLite Xe to the upright position. Plug the
power cord into the receptacle on the back of the unit
and to a power outlet. Connect the USB cable to the
back of the unit and to the computer.
USB Cable
Power Cord
Dichroic splitter
cube door
Dichroic splitter
cube