B SCIENCE B-ULT 100 User manual

B-ULT 100
B-ULT 400 / B-ULT 550
B-ULT 650 / B-ULT 850
USER / SERVICE MANUAL
Copyright © 2017 B Science Global ApS. All rights reserved.

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Dear User
To have efficient use of our product, which has been under quality control, please read the
whole user manual carefully before taking the appliance into use. Save and keep the manual
until the abolition of the appliance.
The information contained in this manual and the accompanying products are copyrighted,
and all rights reserved by B Science Global ApS.
B Science Global ApS reserves the right to make periodic design changes without obligation
to notify any person or entity of such changes.
For the specific unit.
Model name: _______________________________________
Serial number: ______________________________________
Date of installation: __________________________________

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Table of Content
Chapter 1: Introduction.................................................................................................4
1.1 Intended use...................................................................................................................................... 4
Chapter 2: Precondition................................................................................................5
Storage................................................................................................................................................... 5
Environmental protection and disposal. ............................................................................................ 5
Safety instructions. ............................................................................................................................... 6
Chapter 3: Getting Started............................................................................................7
Connection to the mains. ..................................................................................................................... 7
Before use.............................................................................................................................................. 7
Setting up the Freezer. ........................................................................................................................ 7
Electrical supply. ................................................................................................................................... 7
Chapter 4: Refrigerant Safety Precautions .................................................................8
Refrigerant ............................................................................................................................................. 8
Field of application: ................................................................................................................................. 8
Chapter 5: Using the ULT Freezer................................................................................9
Startup process..................................................................................................................................... 9
Operating the Freezer .......................................................................................................................... 9
Temperature control ULT Freezers.................................................................................................. 10
Backup Battery. (For temperature logging and Alarm, during Power cut) ................................. 10
Controller.............................................................................................................................................. 10
1. Main Menu.................................................................................................................................... 10
1. Temp. (Temperature graph) ..............................................................................................................11
2. Status.............................................................................................................................................12
3 Download............................................................................................................................................13
3.1 Select Download Period .................................................................................................................. 13
4. Menu..................................................................................................................................................14
2. Service Menu .....................................................................................................................................15
Test Mode when unit is running ...........................................................................................................16
1. Test Menu when unit is OFF (for troubleshooting)...........................................................................17
Remote Alarm connection ....................................................................................................................20
2. Hysteresis ..........................................................................................................................................20
3. Manual Defrost .................................................................................................................................21
3. Change Pin code for User Menu ....................................................................................................... 22
5. Sound selection.................................................................................................................................23

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6.CALIBRATION...................................................................................................................................... 23
3. User Menu .........................................................................................................................................26
1. Test Mode when unit is running .......................................................................................................27
1. Test Mode .........................................................................................................................................27
2. Device Info ........................................................................................................................................28
3. Update Data ......................................................................................................................................28
4. Change Pin.........................................................................................................................................34
5. Set Time & Date ................................................................................................................................34
6. Set Temperature ...............................................................................................................................35
7. Alarms ...............................................................................................................................................36
1. Alarm List...........................................................................................................................................37
Sensor Menu .........................................................................................................................................37
2. Temperature Alarm settings .............................................................................................................38
3. Temperature Delay ...........................................................................................................................39
4. Door Alarm / Delay............................................................................................................................40
5. Power Alarm......................................................................................................................................41
Main Menu Alarm Screen .....................................................................................................................42
4. Temp. Unit.........................................................................................................................................43
5. Display...............................................................................................................................................44
Chapter 6: Maintaining the ULT Freezer....................................................................45
Cleaning................................................................................................................................................. 45
Manual Defrosting. ............................................................................................................................... 45
Wire diagram B-ULT 100........................................................................................................................46
Wire diagram B-ULT 400 & 650 .............................................................................................................47

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NOTICE: Only use this product for its intended purpose.
Chapter 1: Introduction
Congratulations on the purchase of a B Science ULT Freezer. We trust that this unit will serve
you for many years to come. To gain optimal benefits from your Freezer, please read the
following instructions thoroughly and act accordingly.
The ULT Freezer Premium Line offers many unique features to enhance safety, performance
and ergonomic. To take full advantage of the features, please read this manual firmly and keep
it safe for future reference. If you are unfamiliar with how ULT Freezers operate, please review
Chapter 4: Refrigerant Safety Precautions before you start working with the ULT Freezer. Even
if you are an experienced user of ULT Freezers, please read all the user manual firmly.
1.1 Intended use.
The ULT range –86°C freezer described in this manual are high performance units for
professional use. These products are intended for use as cold storage in research use and as a
general-purpose laboratory freezer, storing samples or inventory at operating temperatures
between –40°C and –86°C. It is not considered a medical device and has therefore not been
registered with a medical device regulatory body. That is, it has not been evaluated for the
storage of samples for diagnostic use or for samples to be re-introduced to the human body.
This unit is not intended for use in classified hazardous locations, nor to be used for the storage
of flammable inventory.
This Manual and other technical information is available in PDF format through email:
[email protected]
or
[email protected]

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Chapter 2: Precondition
Before you install the ULT Freezer, you need to prepare the site for installation. Examine the
location where you intend to install the Freezer. You must be certain that the area is level and of
solid construction.
In addition, a dedicated source of electrical power must be located near the installation site.
Carefully read this chapter to learn:
•Storage requirements.
•Location requirements.
•Electrical power requirements.
•Space requirements.
•Service utility requirements.
•Environment Requirement.
Storage.
If the Freezer will be stored for a period without power, the door and inner doors should be kept
open for free circulation of air inside the cabinet to avoid corrosion of the inner liner.
Environmental protection and disposal.
The packaging is designed to protect the appliance and its components during transportation,
and it is made of recyclable materials.
•Please return the packaging to an official collection point for recycling.
•Old appliances contain reusable materials and should not be disposed off together with
household refuse.
•Remove the spring-action hinges from the appliance, to prevent children from being entrapped in
the appliance.
•Ensure that no part of the refrigeration tubing is damaged as the refrigerant in the appliance risks
leaking to the environment.
•Information about refrigerant type and amount will be found on the Rating plate on the rear of the
appliance (Fig. 1).
Figure: 1

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Safety instructions.
•To prevent injuries and or damage of the appliance, it should be unpacked and set up by
minimum two people.
•If upon unpacking the appliance damage is detected, do not connect to the mains, but contact
the supplier.
•Interference with or repair to the appliance should only be carried out by authorized personnel,
to avoid any injuries. (contact the supplier for further information)
•Never use open flames or other ignition sources inside the appliance.
•Never touch the Freezers interior or products in the Freezer when the Freezer is operating.
Use gloves or alike to avoid injuries (frostbite).
•Keep the key to the doors away from the appliance, and out of the reach of children.

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Chapter 3: Getting Started
Connection to the mains.
•For safety reasons, the appliance must be earthed. If you are in any doubt, please contact an
authorized electrician.
•The appliance should be left OFF for 1 hour before it is connected to the mains. If the
appliance is connected before that, there is a risk of damaging the compressor.
•If for any reason the appliance is disconnected from the mains, please wait 10 minutes before
re-connecting. The electronic starting device needs this time to cool down before a safe re-
start can be made.
Before use.
•Before use, the interior of the appliance should be cleaned with a mild soap solution and
wiped off with a dry clean cloth.
NOTE! Never use any kind of solvent or other chemicals.
Setting up the Freezer.
The Freezer should not be placed where it might be splashed with water, in extreme high humidity or
in direct sunlight. Any of these factors may lead to a reduction in performance and shorten the life
span of the components. The Freezer should be placed on a horizontal level and should not be
placed close to a heating appliance or heating tubes. Allow a minimum of 50 mm (2”) clearance on
the sides, and 100 mm at the back side, to allow the heat from the compressor motor to dissipate.
Underneath the appliance there should be a gap of 15 mm approx. (1⁄2”). On a soft surface, e.g.
carpet, it may be necessary to ensure the correct distance by means of spacers.
Electrical supply.
The electrical supply should always be in accordance with the Rating label on the left side of the
cabinet or at the bottom when you open the door to the chamber.
The supply must always be in accordance with the law and regulations regarding electrical safety, if
any doubts contact your supplier.

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Chapter 4: Refrigerant Safety Precautions
Refrigerant
In the ULT Freezer there are used two type of refrigerants. The cooling system is Cascade cooling.
Carefully read the below description of the refrigerants: R290 and R1150
Demand for information: Personnel working with these refrigerants mentioned above and
below must be educated to know about these safety sheets.
Field of application:
R290 is used as refrigerant in first stage of the cooling system. R290, is refrigerant grade propane, a
natural, refrigerant suitable for use in a range of refrigeration and air conditioning applications. The
use of R290 is increasing due to its low environmental impact and excellent thermodynamic
performance. It is non-toxic with zero ODP (Ozone Depletion Potential) and very low GWP (Global
Warming Potential).
R1150 (+ R290) are used in the second stage of the cooling system.
R1150 Ethylene is refrigerant grade ethylene (ethene), a natural refrigerant suitable for use in very
low temperature refrigeration applications, such as those found in LNG liquefaction processes. R1150
is non-toxic, with zero ODP (Ozone Depletion Potential) and very low GWP (Global Warming
Potential). It is a flammable refrigerant and therefore not suitable for retrofitting existing fluorocarbon
refrigerant systems.
If needed, ask for safety data sheet from your supplier.

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Chapter 5: Using the ULT Freezer
Startup process.
In case the compressors do not start when the Freezer has been plugged in, the electrical
supply may not be in order. Check if there is an electricity supply to the plug or if the fuse is
blown.
Before starting the unit, you must check and decide below parameters:
1. SET TIME: Must change to local time zone.
2. SET TEMP: Change the set point for the desired temperature in the chamber.
3. ALARMS: Change High and Low temperature alarm level.
4. ALARM DELAY: Change the time delay from the temperature reach High or
Low Temperature alarm level, to the alarm will be activated.
5. DOOR OPEN ALARM: Enable the door open alarm and the delay for the alarm will be
activated.
During the Freezer is starting up from Ambient level (RT +20°C), the High Temperature alarm
will be activated several times before the temperature reach the High temperature alarm level.
Operating the Freezer
The Empty Freezer should be switched ON for cooling down the chamber, for at least 8-10
hours, prior to loading ballast into the chamber of the Freezer.
Please note: After the door has been opened, there will be a vacuum created inside the Freezer
due to the low temperatures. Wait a few moments before trying to reopen the door otherwise
the handle or gaskets could be damaged.

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Temperature control ULT Freezers.
The temperature inside the Freezer is controlled by the electronic controller in the top panel.
The controller has a digital readout of the temperature inside the cabinet, and the option of
changing the temperature inside the cabinet.
Backup Battery. (For temperature logging and Alarm, during Power cut)
The battery should be exchanged every two years.
Please note! When commissioning the Freezer, the Battery must be checked for correct
connection.
Controller
1. Main Menu
DISPLAY SHOWS
1. Date
2. Time
3. Temperature inside the cabinet
4. Room Temperature (Ambient)
5. Shows if compressor is running
(it will not be visible if the
compressor is not running)
6. Compressor is running/defrost
7. USB is connected
8. Battery status
DISPLAY GIVES ACCESS
TO
9. Temperature graph
10. Status
11. Download
12. Menu
13. ON/OFF
1
2
3
4
6
7
5
8
9
10
11
12
13

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1. Temp. (Temperature graph)
Temperature graph for 1 day
First picture will show the temperature mapping of the current day.
Temperature graph for 7 days
You can select a Temperature Map for either 1 or 7 days
The second picture show the last 7 days (1 Week) temperature mapping.

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2. Status
1. It shows the working hours of the compressor and the fan.
2. It also shows when the last alarms have been activated.

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3 Download
1. Download button – push to download data
2. Select period of data to download
Every time you download data, you will receive 2 CVS files, a file with temperature data
and a file with the parameter list.
In the USB key, you will find the 2 files under folder “History”
3.1 Select Download Period
1. Select a period that you want to download and press ok.
1. Use arrow up or down to change the value.
2. To save the changed data click on the OK button.
2. Then return to download menu and push the Download button.

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4. Menu
From menu you can gain access to.
1. Admin menu (Only access to authorized personal approved by B Science)
2. Service menu Standard pin code: 0215
3. User menu Standard pin code: 0000
4. Temp unit
5. Display
6. Language (this option is under development) Only English is available.

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2. Service Menu
For access to service menu enter pin code.
1. Select Pin code and click on the OK button.
2. Service Menu
Service menu gives access to
1. Test Mode
2. Hysteresis
3. Manual defrost. (Not use in Ultra Low Temperature freezers)
4. Change pin
5. Sound sel.
6. Calibration

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Test Mode when unit is running
When the unit is Switched ON and running, you can see which relays are ON or OFF and
the temperature of each sensor.
In this example:
The Compressor 1 and Fan 2 is running.
The Defrost heater 2, Door heater 1, Light 1 and Light 2 are ON
Door 1 is open, that is why Fan 1 is OFF and Light 1 and 2 is ON
(Fan stops when door is open.)

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1. Test Menu when unit is OFF (for troubleshooting)
When the unit is Off, you can simulate each relay by pushing the square field, for testing
each kind of device. (Compressor, Light, Fan motor etc.) Very good feature for service and
maintenance use.
In case above the Relays for both Compressors are activated, and the remote Alarm Relay.
You should be able to hear both Compressors are running and check that the Remote
Alarm are activated.

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This feature is for service and maintenance use.
By this Test mode, you can check all relays and
door contacts and check the temperature sensors.
Switch OFF the unit by the display ON/OFF button.
Type in the pin code for one of the pin codes levels,
and push the OK button.
The display will now be in OFF mode.
Click on the MENU button.
Click on the SERVICE button

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Type in the pin code for Service level,
and push the OK button.
Click on the TEST MODE button.
By clicking on each white square angle, the relay
for the specific component will be activated.
In this example, the relay for Compressor 1,
Defrost relay 1, Fan 1, Frame heater 1 and Light 1 are ON.
Alarm output has change from NC to NO or opposite.
The door no. 1 is open.
When finished testing relays, click the HOME button,
for returning to Main screen.
If you do not touch the display, the Test Mode will
automatic stop after 5 minutes for safety reason.