BiOptic Qsep100 User manual

Operation Quick Start
1. Turn on the power.
2. Double click the icon Q-Analyzer™
for Qsep100.
3. Q-Analyzer for Qsep100 software user interface:
4. Click “Connect” to link the instrument.
5. Cartridge and Buffer preparation:
5-3. Alignment Marker preparation for cartridge:
Add ≥ 20 μl “Alignment Marker” into the 0.2 ml tube and then add
10 μl “Mineral Oil” on the top of Alignment Marker.
5-2. Add 1X Separation Buffer into “S” well
and add diH2O into others.
* Buffer height should be equal to the
groove of wells.
5-1. Unpacking Cartridge:
Please follow the steps of “Unpacking guide” inside the cartridge
box.
Packing list of Cartridge kit:
Cartridges
Alignment Marker
Mineral Oil
Dilution Buffer
Separation Buffer
Buffer Tray
Droppers
0.2 ml tubes
6. Click “Change Buffer”.
7. Place Alignment Marker 20 bp &
1000 bp C109100 (≥ 20 μl) at MA-1
position and the buffer tray on the
tray holder.
For other size of Alignment Marker,
please place to the corresponding
position.
* Please make sure buffer tray have
been placed correctly and fitted in
the holder.
* Spin down and make sure the
Alignment Marker is in the bottom
of tube and no air bubble appears.
8. Hold the tray holder by your hand
and press down the tube of
Alignment Marker tightly into the
well by thumb.
9. Click “Change Sample” and place your samples. (≥ 20 μl)
* Spin down and make sure the sample is in the bottom
of tube and no air bubble appears.
10. Open the cartridge door and insert
cartridge.
(Guiding groove facing front)
11. Close the cartridge door.
* Press the door and make sure
you hear the squeeze sound .
12. Click “Latch”.
Cartridge information will be presented on the main
window after the cartridge is latched.
Bio–Fragment Analyzer : Qsep100 Document No.: F0004
Ver.: E-2021
Page: 1/3
(P) (W)
(S) (C)

Operation Quick Start
14. Click “Run” to start the analysis.
13-2. Click “Method” to select analysis method.
* If you want to analyze the samples with Alignment Marker, please
choose the appropriate Alignment Marker and then place it on the
corresponding position. (Check √the box)
13-3. Enter the result name.
12-1. New Cartridge Calibration:
New cartridge need to be calibrated before using.
Please proceed with the following steps.
1. Click “OK”.
2. Click “HV Check”.
3. Click “Calibrate”.
* The storage and transportation
condition may influence the gel-
matrix and cause unstable current.
Check current and see if it’s stable or
not. After HV Check, if current is still
unstable, please repeat this step 2-3
times.
•For troubleshooting, please refer to cartridge unpacking guide for
details.
Bio–Fragment Analyzer : Qsep100
During Calibration
Software will recognize peaks from Alignment Marker signal. DO
NOT use Size Marker or DNA sample doing “Calibrate”.
Brief introduction of the Signal Chart:
Current (gray line)
Fluorescence Intensity (red line)
Current Unit
Relative Fluorescence Unit
Document No.: F0004
Ver.: E-2021
Page: 2/3
Please refer to operation manual for details.
*Please check the Alignment Marker has
been located in correct position.
13. Click on the blank column and ①designate the sample
locations, ②test method, sample duration, runs, ③
result name and ④Para by following steps 13-1, 13-2,
13-3 and 13-4.
13-4. Click the icon “Para” and you can set the parameters.
(Baseline Factor, Peak Threshold, Calculate…etc.)
13-1. Click the “Sample Position”, mark the position of sample on the
plate and then press “OK”.

Operation Quick Start
Contact Information:
Company Name: BiOptic Inc.
Office Address: (23141) 5F., No.6, Ln. 130, Minquan Rd., Xindian Dis., New Taipei City, Taiwan
Factory Address: (23141) 4F., No.108-3,Minquan Rd., Xindian Dist., New Taipei City, Taiwan
Tel: [phone], Fax: [phone], E-mail: [email protected]
Bio–Fragment Analyzer : Qsep100 Document No.: F0004
Ver.: E-2021
Page: 3/3
Introduction of the Control Panel
A: System Control
B: Cartridge Control
C: Information Area
D: Testing Status
E: Testing Sequence
Finished
Proceeding
Number: Remaining runs
Pending
Number: Remaining runs
Prefix of the result name:
Tool tip will show up the previewing of the result name.
Prefix of the result name:
Cartridge Number:
Sequence Number/Position/Runs
D
E