Thermo Scientific NanoDrop OneC User manual

NanoDrop Micro-UV/Vis Spectrophotometer
NanoDrop One
C
with
NanoDrop QC Software
User Guide
269-342200 NanoDrop QC UG Revision A December 2019

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Thermo Scientific NanoDrop OneCwith NanoDropQC Software User Guide iii
C
Chapter 1 About the Spectrophotometer. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1
Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Touchscreen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Cuvette Holder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
USB-A port . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Accessories. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
PR-1 Pedestal Reconditioning Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
PV-1 Performance Verification Solution . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Instrument Detection Limits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Chapter 2 Instrument Set up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7
Register Your Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Update Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Setting Up User Account Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
User Account Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Security Administration Policies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Technical Support . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Chapter 3 Applications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11
Measure UV-Vis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Measure UV-Vis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
To make UV-Vis measurements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Best practices for UV-Vis measurements . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
UV-Vis Reported Results. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Settings for UV-Vis Measurements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Measure Chemometrics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
Measure Chemometrics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
Create Chemometrics Method. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Edit Chemometrics Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Chemometrics Reported Results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Settings for Chemometric Measurements . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
Contents

Contents
iv NanoDrop OneCwith NanoDropQC Software User Guide Thermo Scientific
Measure Custom . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Measure using a Custom Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Delete Custom Method. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Custom Method Reported Results. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
Manage Custom Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Chapter 4 Learning Center . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .45
Micro-Volume Sampling—How it Works. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
Set Up the Instrument. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
Measure a Micro-Volume Sample . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
Measure a Sample Using a Cuvette . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
Prepare Samples and Blanks. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
Basic Instrument Operations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
NanoDrop One Home Screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
NanoDrop One Measurement Screens . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
NanoDrop One General Operations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90
Instrument Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100
Data Deletion Settings. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105
PC Control Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 107
PC Control Home Screen overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 107
Control options . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 108
History . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 108
Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 109
Chapter 5 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .111
Maintenance Schedule. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112
Cleaning the Touchscreen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
Maintaining the Pedestals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 114
Cleaning the Pedestals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 114
Reconditioning the Pedestals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 116
Decontaminating the Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 119
Maintaining the Cuvette Sampling System . . . . . . . . . . . . . . . . . . . . . . . . . . . 121
Instrument Diagnostics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 122
Intensity Check . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 122
Performance Verification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 124
Pedestal Image Check . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 130
Chapter 6 Safety and Operating Precautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .131
Operating Precautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 132
Safety Information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 133

Thermo Scientific NanoDrop OneCwith NanoDropQC Software User Guide 1
1
About the Spectrophotometer
The Thermo Scientific™ NanoDrop™ OneCis a compact, stand-alone
UV-Visible spectrophotometer developed for micro-volume analysis of a wide variety of
analytes. The patented sample retention system enables the measurement of highly
concentrated samples without the need for dilutions.
The NanoDrop OneCsystem comes with preloaded software and a touchscreen display.
NanoDrop QC PC Control software can be installed on a local PC and used to control the
instrument and view data. The instrument can be connected to an optional printer with a
USB cable or to a remote printer through an Ethernet connection or wireless network.
NOTICE Locate the instrument away from air vents and exhaust fans to minimize
evaporation
NOTICE Before operating a NanoDrop One instrument, please read the safety and
operating precautions and then follow their recommendations when using the instrument.
NanoDrop OneCSpectrophotometer
Arm
Pedestal
Cuvette
holder

1About the Spectrophotometer
Features
2NanoDrop OneCwith NanoDropQC Software User Guide Thermo Scientific
Features
TheNanoDrop OneCspectrophotometer features the patented micro-volume sample
retention system. The NanoDrop OneCalso features a cuvette holder for analyzing dilute
samples using standard UV-visible cuvettes.
Touchscreen
TheNanoDrop OneCcomes with a built-in, 7-inch high-resolution touchscreen preloaded
with easy-to-use instrument control software. The touchscreen can slide left or right to
accommodate personal preference, and tilt forward or back for optimal viewing

1 About the Spectrophotometer
Features
Thermo Scientific NanoDrop OneCwith NanoDropQC Software User Guide 3
Cuvette Holder
The NanoDrop OneCincludes a cuvette holder for measuring dilute samples, colorimetric
assays, cell cultures and kinetic studies. The cuvette system has these features:
• extended lower detection limits
• 37 °C heater option for temperature-sensitive samples and analyses
• micro-stirring option to ensure sample homogeneity and support kinetic studies
For details, see Measure a Sample using a Cuvette.
USB-A port
Two more USB-A ports are located on instrument back panel
Instrument light path
Cuvette holder

1About the Spectrophotometer
Accessories
4NanoDrop OneCwith NanoDropQC Software User Guide Thermo Scientific
Accessories
This section lists the accessories included for use with the NanoDrop OneC.
PR-1 Pedestal Reconditioning Kit
PV-1 Performance Verification Solution
Liquid photometric standard used to check instrument performance. For more information,
see Performance Verification.
Specially formulated conditioning compound that
can be applied to the pedestals to restore them to a
hydrophobic state (required to achieve adequate
surface tension for accurate sample measurements).
The kit includes conditioning compound and
applicators. For more information, see
Reconditioning the Pedestals.

1 About the Spectrophotometer
Instrument Detection Limits
Thermo Scientific NanoDrop OneCwith NanoDropQC Software User Guide 5
Instrument Detection Limits
Measurement Location Pathlength (mm)
Upper Detection Limit
(10 mm Equivalent Absorbance)
Pedestal 1.0 12.5
0.2 62.5
0.1 150
0.05 300
0.03 550
Cuvette 10 1.5
53
2 7.5
115

1About the Spectrophotometer
6NanoDrop OneCwith NanoDropQC Software User Guide Thermo Scientific
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