Biosan ES-20 User manual

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Contents
1. Safety Precautions...................................................................................................3
2. General information..................................................................................................4
3. Getting started..........................................................................................................5
4. Operation .................................................................................................................6
5. Specifications...........................................................................................................8
6. Maintenance.............................................................................................................9
7. Warranty and Claims. Registration.........................................................................10
8. EU Declaration of Conformity.................................................................................11

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1. Safety Precautions
The following symbols mean:
Caution: Make sure you have fully read and understood the present Manual
before using the equipment. Please pay special attention to sections
marked by this symbol.
GENERAL SAFETY
Use only as specified in the Operating Manual provided.
Do not use a dropped or damaged unit.
Store and transport the unit in a horizontal position (see package label).
After transportation or storage and before connecting to electric circuit, keep the unit
under room temperature for 2-3 h.
Before using any cleaning or decontamination methods except those recommended
by the manufacturer, check with the manufacturer that the proposed method will not
damage the equipment.
Do not make modifications to the design of the unit.
ELECTRICAL SAFETY
Connect only to electric circuit with voltage corresponding to that on the serial number
label.
Ensure that the switch and plug are easily accessible during use.
Do not plug the unit into an ungrounded power socket, and do not use an ungrounded
extension lead.
Disconnect the unit from electric circuit before moving. Switch the unit off and
disconnect the power cord plug frompower socket to disconnect the unit from electric
circuit.
If liquid penetrates into the unit, disconnect it from electric circuit and have it checked
by a repair and maintenance technician.
Do not operate the unit in premises where condensation can form. Operating
conditions of the unit are defined in the Specifications section.
DURING OPERATION
Do not impede the platform motion.
Do not load over the maximum load value mentioned in the Specifications section of
these operating instructions.
Do not leave the operating unit unattended.
Do not operate the unit in environments with aggressive or explosive chemical
mixtures. Please contact manufacturer for possible operation of the unit in specific
atmospheres.
Do not operate the unit if it is faulty or has been installed incorrectly.
Do not use outside laboratory rooms.
BIOLOGICAL SAFETY
It is the user’s responsibility to carry out appropriate decontamination if hazardous
material is spilt on or penetrates into the equipment.

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2. General information
The ES-20 is a compact desktop shaker-incubator used for mixing of biological liquids
as well as incubation and cultivation of biological liquids according to the program set by the
operator.
Built-in microprocessor thermocontroller provides constant temperature control in the
incubator chamber. Forced heated air circulation inside the transparent Plexiglas chamber
guarantees even temperature distribution. Dismountable construction makes transportation
easy. Orbital shaking is controlled by the digital tachometer (rpm) and digital timer
regardless of the temperature. The unit is equipped with the direct-drive system ensuring
most reliable stable long-time operation (up to 30 of 24 hour cycles).
The ES-20 is easy to operate. The two-line LCD display shows both set and actual
values for temperature, time and speed.
The device consists of a thermal regulator and control block, shaking block and a
Plexiglas chamber.
The elements located on the front panel:
- power switch (fig.1/);
- display (fig.1/);
- control buttons (fig.1/).
The elements located on the rear panel:
- shaking block control cable (fig.1/);
- socket for connecting the power cord
(fig.1/);
- fuse holder (fig.1/).
Fig.1. Overall view
Five interchangeable platform types allow using the shaker for:
Growing cell cultures in flasks and other laboratory glassware.
Extracting tissue samples at physiological temperatures.
Other sample preparation processes.
The unit is applicable in all the areas of clinical, biotechnology and microbiology
laboratory research.

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3. Getting started
3.1. Unpacking.
Remove packing materials carefully and retain for them future shipment or storage of
the unit. Examine the unit carefully for any damage incurred during transit. The
warranty does not cover in-transit damage. Warranty covers only the units
transported in the original package.
3.2. Complete set. Package contents:
Standard set:
- Shaker-Incubator ES-20 ................................................................................1 piece
- Power cord.....................................................................................................1 piece
- Control cable..................................................................................................1 piece
- spare fuse (inside the fuse holder) ................................................................1 piece
- Operating manual, Certificate .........................................................................1 copy
- ES-20 Shaker-Incubator Assembling Instruction.............................................1 copy
Optional accessories:
- UP-12 platform ......................................................................................on request
- PP-4 platform ........................................................................................on request
- P-12/100 platform .................................................................................on request
- P-6/250 platform ...................................................................................on request
- P-16/88 platform ...................................................................................on request
- HB-200 holding bar ...............................................................................on request



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3.3. Setup.
- Assemble the unit, using the enclosed ES-20 Orbital Incubator Shaker Assembling
Instruction as a guide.
- Place the unit on a horizontal even working surface.
- Remove protective film from the display.
- Connect the shaker control cable (fig.1/) into the corresponding sockets on the
rear of the thermostatic and shaking blocks.
- Plug the mains cable into the socket on the rear (fig.1/) and position the unit so
that there is easy access to the power switch and connector.
3.4. Platform installation.
- Install the platform by inserting the pins on the bottom side of the platform into the
holes on the supporting platform on the shaker.
Note! Move the unit carefully and only in a vertical position.
4. Operation
Fig.2 Control panel
4.1. Connect the unit to a grounded power socket. Set the Power switch to position I (ON).
4.2. The display will turn on with the upper line (Set p.) showing the previously set time,
speed and temperature and the lower line (Actual p.) showing current readings of
the same parameters (STOP –time, 000 –speed and incubator temperature °C,
which automatically starts rising according to the temperature set in the upper line).
4.3. Setting the parameters. Use the readings in the upper line of the display (Set p.),
while setting the parameters required. The set parameters can also be changed
during operation.
4.3.1. Setting time (Time). Using the ▲ and ▼ Time keys (Fig. 2/1), set the required
working time interval in hours and minutes (increment - 1 min). Pressing the key for
more than 3 seconds will increase the increment.
4.3.2. Setting speed (RPM). Using the ▲ and ▼ RPM keys (Fig. 2/2), set the required
shaking speed (increment 10 RPM). Pressing the key for more than 3 seconds will
increase the increment.

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4.3.3. Setting temperature (Temp.°C).Using the ▲ and ▼ Temp. keys (Fig. 2/3), set the
required temperature (increment 0.1°C). Pressing the key for more than 3 seconds
will increase the increment.
Caution! The chamber heating can be turned off only by setting the
temperature value below 25 °C (the display will show OFF –
Temp.°C–Set p.).
4.4. Program execution.
4.4.1. Place samples on the platform.
4.4.2. Press the Shaker-Run/Stop key (Fig. 2/4). The platform will start rotating and the
timer indicator will start counting the time interval (with 1 min precision).
Note! If the rotation speed is set to zero, pressing Shaker-Run/Stop key
starts the timer but the platform does not move.
4.4.3. After finishing the program (after the set time elapses), the platform motion will stop
and the timer will be showing the flashing reading STOP accompanied by the
repetitive sound signal until the Shaker-Run/Stop key is pressed.
Caution! At the end of the set time period the platform movement is stopped
automatically, but the heating can be stopped ONLY manually by
reducing the temperatureusing the ▼Temp. key (Fig. 2/3 –the lower
key) till the OFF sign appears in the upper line of the display.
4.5. If the working time is not set (the timer indicator in the upper line shows 00:00),
pressing the Shaker-Run/Stop key will start continuous operation of the unit (with
countdown timer in the lower line and indication OFF in the upper line) until the
Shaker-Run/Stop key is pressed again.
4.6. The timer can be reset during operation if required. Press the Timer-Run/Stop key
twice (Fig. 2/5), first –to stop the timer, second –to restart the timer.
4.7. The platform motion can be stopped at any time by pressing the Shaker-Run/Stop
key. In this case, the program execution and the platform motion will stop and the
timer will switch into the STOP mode saving previously set time. Press the Shaker-
Run/Stop key to repeat the operation with the same time and speed.
4.8. After finishing the operation, set the power switch, located on the rear panel of the
unit, in position O (Off) and disconnect the external power supply with from electric
circuit.

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5. Specifications
The unit is designed for operation in cold rooms, incubators (except CO2incubators)
and closed laboratory rooms at ambient temperature from +4°C to +40°C in a non-
condensing atmosphere and maximum relative humidity 80% for temperatures up to 31°C
decreasing linearly to 50% relative humidity at 40°C.
Temperature setting range ...............................................................+25°C ... +42°C
Temperature control range * ........................................5°C above ambient ... +42°C
Temperature setting resolution .........................................................................0.1°C
Speed control range ..............................................50–250 RPM (increment 10 rpm)
Maximum load..................................................................................................2.5 kg
Orbit ...............................................................................................................10 mm
Digital time setting..................................................1 min –96 hrs (increment 1 min)
Continuous operating time, not more ...........................................................30 days
Recommended interval between operation sessions not less than 8 hours
Display ........................................................................................... 2x16 signs, LCD
Dimensions of the inner chamber ................................................. 305x260x250 mm
Dimensions .................................................................................. 340x340x435 mm
Current/power consumption ...............230 V, 0.7 A / 160 W or 120 V, 1.6 A / 170 W
Voltage .............................................................230 V, 50/60 Hz or 120 V, 50/60 Hz
Weight**.........................................................................................................13.2 kg
Optional
accessories
Description
Catalogue
number
PP-4
Metallic flat platform with non-slip rubber mat.
Working dimensions 215x215 mm
BS-010108-BK
UP-12
Universal platform with adjustable bars for different
types of flasks, with non-slip rubber mat. Working
dimensions 265x185 mm
BS-010108-AK
HB-200
Additional holding bar for PUP-12
BS-010108-FK
P-12/100
Platform with clamps for flasks, 100 ml (12 places).
Working dimensions 250x190 mm
BS-010108-EK
P-6/250
Platform with clamps for flasks, 250 ml (6 places).
Working dimensions 250x190 mm
BS-010108-DK
P-16/88
Platform with spring holders for up to 88 tubes up to
30 mm diameter (e.g. 10 ml, 15 ml, 50 ml tubes).
Working dimensions 275x205x75 mm
BS-010116-BK
Biosan is committed to a continuous program of improvement and reserves the right
to alter design and specifications of the equipment without additional notice.
* In cold room, the unit provides stable thermostatic temperature control 30°C above room temperature.
** Accurate within ±10%.

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6. Maintenance
6.1. If the unit requires maintenance, disconnect the unit from the electric circuit and
contact Biosan or your local Biosan representative.
6.2. All maintenance and repair operations must be performed only by qualified and
specially trained personnel.
6.3. Standard ethanol (75%) or other cleaning agents recommended for cleaning of
laboratory equipment can be used for cleaning and decontamination of the unit.
6.3.1. The door and side panels are made of organic glass (polymethyl methacrylate,
Plexiglas®) and are prone to scuffing and scratches if improperly cleaned. Care during
cleaning of the door and side panels is recommended to reduce wear of the Plexiglas
surface.
Caution! Clean the door and side panels with a mild detergent; never use
organic based compounds, pure alcohol, alcohol-containing cleaners
(more than 15%) or ammonia containing cleaners for Plexiglas! Do
not use abrasive pads or cleansers.
6.3.2. For decontamination, it is recommended to use a special DNA/RNA removing
solution (e.g. DNA-Exitus Plus™, RNase-Exitus Plus™).
The table below shows the interreaction of Plexiglas with ethyl alcohol and other
solutions.
Liquid
Interreaction with Plexiglas
DNA-Exitus Plus™
No reaction.
RNase-Exitus Plus™
No reaction.
Ethyl alcohol 10-15%
No reaction.
Ethyl alcohol 30%
Limited reaction
Ethyl alcohol Pure
Full reaction. Do not use!
H2O2 6%
No reaction.
6.4. Fuse replacement
Disconnect from electric circuit.
Remove the power plug from the rear of the unit.
Pull out the fuse holder by applying leverage in recess (A).
Remove the fuse from the holder. Check and replace with the correct fuse if
necessary: for 230 V, M 1 A or for 120 V, M 2 A (type M- time lag: Medium).
Fig.4. Fuse holder

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7. Warranty and Claims. Registration
7.1. The Manufacturer guarantees the compliance of the unit with the requirements of
Specifications, provided the Customer follows the operation, storage and trans-
portation instructions.
7.2. The warranted service life of the unit from the date of its delivery to the Customer is
24 months (excluding platforms mentioned in the table on page 8). For extended
warranty, see p. 7.5.
7.3. Warranty covers only the units transported in the original package.
7.4. If any manufacturing defects are discovered by the Customer, an unsatisfactory
equipment claim shall be compiled, certified and sent to the local distributor ad-dress.
To obtain the claim form, visit section Technical support on our website at link
below.
7.5. Extended warranty. For ES-20, a Basic Plus class model, extended warranty is a paid
service. Contact your local Biosan representative or our service department through
the Technical support section on our website at the link below.
7.6. Description of the classes of our products is available in the Product class de-
scription section on our website at the link below.
Technical support
Product class description
biosan.lv/en/support
biosan.lv/classes-en
7.7. The following information will be required in the event that warranty or post-warranty
service comes necessary. Complete the table below and retain for your records.
Model
ES-20, Orbital shaker-incubator
Serial number
Date of sale

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8. EU Declaration of Conformity
Unit type Shakers-incubators
Models ES-20, ES-20/60
Serial number 14 digits styled XXXXXXYYMMZZZZ, where XXXXXX is
model code, YY and MM –year and month of production,
ZZZZ –unit number.
Manufacturer SIA BIOSAN
Latvia, LV-1067, Riga, Ratsupites str. 7/2
Applicable Directives EMC Directive 2014/30/EC
LVD Directive 2014/35/EC
RoHS2 2011/65/EC
WEEE 2012/19/EU
Applicable Standards LVS EN 61326-1: 2013
Electrical equipment for measurement, control and
laboratory use. EMC requirements. General requirements.
LVS EN 61010-1: 2011
Safety requirements for electrical equipment for
measurement, control, and laboratory use. General
requirements.
LVS EN 61010-2-010: 2015
Particular requirements for laboratory equipment for the
heating of materials.
LVS EN 61010-2-051: 2015
Particular requirements for laboratory equipment for mixing
and stirring.
We declare that this product conforms to the requirements of the above Directives
____________________ ____________________
Signature Signature
Svetlana Bankovska Aleksandr Shevchik
Managing director Engineer of R&D
____________________ ____________________
Date Date

Biosan SIA
Ratsupites 7, build. 2, Riga, LV-1067, Latvia
Phone: [phone]
Fax: [phone]
http://www.biosan.lv
Edition 2.04 - December 2016