Burleigh PCS-6000 Series User manual

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OPERATING MANUAL
PCS-6000 SERIES
Motorized Patch-Clamp
Micromanipulator
035-00266 (Rev. 0)
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All rights reserved. No part of this publication may be reproduced, stored in a
retrieval system, or transmitted in any form, be it electronically, mechanically
or by any other means such as photocopying, recording or otherwise, without
the prior written permission of EXFO Photonic Solutions Inc. (EXFO).
Information provided by EXFO is believed to be accurate and reliable.
However, no responsibility is assumed by EXFO for its use or for any
infringements of patents or other rights of third parties that may result from
its use. No license is granted by implication or otherwise under any patent
rights of EXFO.
The information contained in this publication is subject to change without
notice.
© 2007 EXFO Photonic Solutions Inc.
Words that EXFO considers trademarks have been identified as such. EXFO®,
Burleigh®and Gibraltar®are registered trademarks. However, neither the
presence nor absence of such identification affects the legal status of any
trademark.
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CONTENTS
1. Introduction……………………………………………………………………………………………..1-1
Getting Started ...............................................................................................................................1-1
Overview ..........................................................................................................................................1-1
Major Components .........................................................................................................................1-2
Micromanipulator Assembly .................................................................................................1-2
Axis Control Unit.....................................................................................................................1-2
Joystick.....................................................................................................................................1-3
Controller Unit.........................................................................................................................1-3
Glossary of Symbols …………………………………….................................................................1-3
Safety Warnings .............................................................................................................................1-4
2. Description……………………………………………………………………………………………….2-1
Mechanisms of Motion .......................................................................................................................2-1
Coarse Motion .........................................................................................................................2-1
Fine Motion ..............................................................................................................................2-1
Configurations ....................................................................................................................................2-2
Axes of Motion ...........................................................................................................................2-2
Right and Left Configurations ..............................................................................................2-3
Manipulator Configurations ..................................................................................................2-3
3. Orientation……………………………………………………………………………………………….3-1
Control Unit......................................................................................................................................3-1
Front Panel...............................................................................................................................3-1
Rear Panel………………………………………………………………………………………….....3-3
Axis Control Unit (ACU).................................................................................................................3-4
Joystick .............................................................................................................................................3-5
Graphical User Interface…………………………………………………………………………………3-6
Serial Port Communication………………………………………………...................................3-11
4. Installation ..................................................................................................................................4-1
Unpacking the Unit.........................................................................................................................4-1
Removing the Shipping Clips .......................................................................................................4-1
Mounting the Manipulator.............................................................................................................4-2
Connecting the Cables...................................................................................................................4-2
Mounting a Headstage and Pipette.............................................................................................4-3
5. Operation.....................................................................................................................................5-1
Powering Up and Checking for Motor Motion ...........................................................................5-1
Positioning Under the Microscope and Checking for Piezo Motion.......................................5-1
Adjusting the Approach Axis Angle ............................................................................................5-2
Setting the Adjustable Stop .........................................................................................................5-3
Operational Hints............................................................................................................................5-3
Joystick Control.......................................................................................................................5-3
Piezo Control ...........................................................................................................................5-4
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Single Stepping ...................................................................................................................... 5-4
Drift Problems......................................................................................................................... 5-4
6. Maintenance ............................................................................................................................... 6-1
Overview of Maintenance Required............................................................................................ 6-1
Maintenance Schedule .......................................................................................................... 6-1
Lubricating Motor Shafts ...................................................................................................... 6-1
Electronic Maintenance......................................................................................................... 6-6
Replacing the Fuse(s) ........................................................................................................... 6-6
Adjusting the Friction of the ACU Knobs................................................................................... 6-7
Adjusting the Friction and Eliminating Lateral Play in the Manual Adjustment Knobs ... 6-8
Troubleshooting.............................................................................................................................. 6-9
Problem: Excess motion of pipette tip during manual adjustment ........................... 6-9
Problem: No fine motion................................................................................................... 6-10
Problem: No coarse motion.............................................................................................. 6-11
Problem: Drift...................................................................................................................... 6-12
7. Specifications ............................................................................................................................. 7-1
Performance: .......................................................................................................................... 7-1
Environmental Specifications:............................................................................................. 7-1
Product Safety: ...................................................................................................................... 7-2
WEEE Directive Compliance:................................................................................................7-3
Warranty
Index
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Burleigh PCS-6000 Series Operating Manual 1 Introduction
EXFO Life Sciences & Industrial Division
1-1
1. INTRODUCTION
Getting Started
Thank you for purchasing a PCS-6000 Series motorized manipulator. This
manual is intended to provide in-depth information on the operation and use
of the PCS-6000.
Overview
Burleigh PCS-6000 Series patch-clamp micromanipulators from EXFO Life &
Industrial Division are ultra-stable, high-resolution positioning systems
designed for patch clamp recording. EXFO has incorporated more than 20
years of experience and knowledge in piezoelectric actuators, precision
mechanics and electronic control to create a system that maximizes your
research productivity.
The Burleigh PCS-6000 micromanipulator is mounted on or next to your
microscope. Your headstage and pipette are mounted to the Burleigh PCS-
6000 so that the end of the pipette is in the field of view of the microscope.
The Burleigh PCS-6000 provides rapid motorized positioning with travel range
of about 25 mm and steps as small as 5 µm. After preliminary positioning
with the motors, fine positioning is obtained by using piezo actuators, with
150 µm or 300 µm travel. Special rotational adjustments are incorporated to
allow optimum pipette working angle and easy pipette exchange. The
Burleigh PCS-6000 is modular and allows numerous configurations so that it
can be customized for each user.
The motor is controlled by a joystick that is separate and distinct from the
fine motion control. The farther the joystick is moved from its rest position,
the faster the stage moves. The fastest speed attainable is 2 mm/second.
The fine motion of the piezo is controlled by the position of a large knob on
the axis control unit (ACU). The knobs can be turned through 1080 degrees
(three revolutions) to sweep the entire range of the piezo movement, either
150 or 300 µm. When the knob is left in a specific position, motion stops.
While the Burleigh PCS-6000 was designed for patch clamp recording, the
characteristics of the instrument are useful for many other applications
requiring high-precision positioning capabilities.
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Burleigh PCS-6000 Series Operating Manual 1 Introduction
EXFO Life Sciences & Industrial Division
1-2
Figure 1-1: PCS-6000 Series patch-clamp micromanipulator
Major Components
Micromanipulator Assembly
The micromanipulator assembly consists of up to four linear stages and two
rotary pivot stages assembled with a 90°bracket. Depending on the
configuration, the linear stages can have no piezo, a 150 µm piezo or a 300
µm piezo, no long-travel adjustment mechanism, a manual long travel
adjustment screw or a motor.
Axis Control Unit
The axis control unit (ACU) is a rectangular box with large rotary knobs that
control the position of the piezo actuators. Normally it is placed next to the
microscope.
Axis Control
Unit
(
ACU
)
Joystick
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Burleigh PCS-6000 Series Operating Manual 1 Introduction
EXFO Life Sciences & Industrial Division
1-3
Joystick
The joystick controls the speed and direction of the servo motors. The joystick
assembly also includes switches to activate specific functions.
Controller Unit
The control unit contains the electronics that drive the piezo and the servo
motors.
Glossary of Symbols
The following safety related symbols are used on this product and throughout
this manual:
CAUTION – RISK OF DANGER
Consult accompanying documentation prior to
operation
CAUTION
Risk of Electrical Shock – Do not remove cover, no
user serviceable parts inside
CAUTION
Indicates a potentially hazardous situation that, if
not avoided, may result in component damage. Do
not proceed unless you understand and meet the
required conditions.
Chassis Ground

Motor Direction Switch
Input/Output Control Signal
Fuses – User Replaceable
Safety Ground
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Burleigh PCS-6000 Series Operating Manual 1 Introduction
EXFO Life Sciences & Industrial Division
1-4
Safety Warnings
WARNING
Should this equipment be used in a manner not specified
by EXFO, the protection provided by the equipment may
be impaired. Always follow the operating instructions as
indicated!
WARNING
Refer servicing to qualified personnel. To prevent
electrical shock, do not open or remove the cover of the
controller or joystick.
WARNING
IEC Class I Product: Use only the 3-prong AC power cord
supplied with this equipment. Always insert the power
cord into an electrical outlet provided with a protective
earth contact.
WARNING
To reduce the risk of fire or shock, always replace the
fuses with the same type and rating. Always detach the
power supply cord prior to attempting to replace fuses!
WARNING
This unit contains high voltage components. It is
recommended that only qualified technical personnel
perform testing or repairs.
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Burleigh PCS-6000 Series Operating Manual 2 Description
EXFO Life Sciences & Industrial Division
2-1
2. DESCRIPTION
Mechanisms of Motion
PCS-6000 linear stages integrate piezo-flexure assemblies inside crossed
roller bearing linear stages with a stepping motor for coarse motion. The
joystick is used to control the coarse motion. A large knob on the axis control
unit (ACU) controls the fine positioning obtained by use of a piezo. Two
rotary pivot stages are incorporated into the manipulator to allow optimum
adjustment of the pipette working angle and easy pipette exchange.
Coarse Motion
Coarse motion is provided by a stepping (servo) motor attached to the linear
translation stage. The motor is controlled by a joystick that has a “dead
zone” in the rest position. This is a safety feature so that a small accidental
touch does not cause motion. The farther away from the neutral position the
joystick is moved, the faster the motor moves the stages. The direction of
motion of the motor is controlled by the direction of the joystick motion. It is
possible to move along a diagonal line if the joystick is moved along a
diagonal.
With a bit of practice an experienced operator can learn to position the pipette
to within about 15 µm of the target with the coarse motion provided by the
motor.
Fine Motion
The piezo-flexure stages integrated into the linear stage provide fine motion.
These stages contain a piezoelectric ceramic element that expands when a
voltage is applied across it. The piezoelectric element is mounted in a
specially machined metal body that provides amplification of the motion.
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