CBS 2301 User manual

www.custombiogenics.com
74100 Van Dyke wBruce Twp., MI 48065 wUSA
1.800.523.0072 wTel: 1.586.331.2600 wFax: 1.586.331.2588
Leading the World with Innovative Cryopreservation Technology Solutions
EC REP
European Authorized Representative
EMERGO GROUP
Molenstraat 15
2513 BH, The Hague
The Netherlands
CUSTOM BIOGENIC SYSTEMS
2301 LN2 STORAGE UNIT
USER MANUAL

Custom Biogenic Systems liquid nitrogen freezers equipped with 2301 controllers are CE marked to the
Medical Device Directive. The Medical Device Directive is a European Union directive regulating medical device
construction and operation.
Custom BioGenic Systems
74100 Van Dyke Road
Bruce Township, MI 48065
European Authorized Representative
EMERGO GROUP
Molenstraat 15
2513 BH, The Hague
The Netherlands
Custom Biogenic Systems maintains ISO 13485:2003 Quality Management System
This manual covers the use of Custom Biogenic Systems Liquid Nitrogen Freezers equipped with 2301
controllers. This equipment is intended for use by trained personnel only. READ THE MANUAL BEFORE USING
THIS EQUIPMENT.
Custom Biogenic Systems Liquid Nitrogen Freezers covered in this manual are non-hazardous, open mouth
vacuum insulated dewars specically designed for use with liquid nitrogen. They are not subject to any
pressure vessel codes as they are open to atmospheric pressure. They are shipped empty without liquid
nitrogen or any hazardous material from our factory in Michigan
0086
Custom Biogenic Systems liquid nitrogen freezers, cryogenic equipment and accessories are designed
and intended for use with various applications where extremely low temperature storage is desired, such
as veterinarian, horticultural, pharmaceutical, industrial, scientic and research laboratories. They are not
intended for use in the diagnosis of disease or other conditions, or in the cure, mitigation, treatment or
prevention of disease, and as such have no medical purpose. They are designed for, but not limited to, use
in the laboratory environment.

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Thank you for choosing Custom BioGenic Systems
Custom BioGenic Systems can supply you with everything you need to eectively and safely store your samples.
Our 2101 controlled rate freezer will freeze your samples down at a controlled rate prior to long term liquid
nitrogen storage.
We have an extensive selection of freezer racks, bag storage solutions, boxes and dividers for liquid nitrogen
freezers, mechanical freezers as well as custom congurations.
For long term storage we oer our patented Isothermal dry storage liquid nitrogen freezers, carousel freezers,
standard liquid nitrogen freezers, cryosystems and dewars in many popular sizes.
VersAlert will monitor your temperature critical products and send alerts via text message or email if specied
parameters are exceeded.
For more information please contact Custom BioGenic Systems at 1.800.523.0072, www.custombiogenics.com
or email [email protected]om.

We at Custom BioGenic Systems are proud of our work, and appreciate your purchase
of this product. With proper care, this equipment will be trouble-free for many years to come.
Before setting up and using your new cryogenic storage unit, rst check to see that all parts
are accounted for and that no damage has occurred during shipping. Also, read this manual
completely before proceeding to set-up. If at any time you are unsure of the procedures for
set-up and use of this product, please contact CBS or your CBS sales representative.
NOTE: If equipment is used in a manner not specied by Custom BioGenic Systems,
the protection provided by the equipment will be impaired.
PRODUCT WARRANTY
Custom BioGenic Systems warrants all manufactured cryogenic equipment to
be free from defects in workmanship or materials for a period of ve year vacuum
warranty and a 2 year warranty on electronics and parts.
Custom BioGenic Systems’ liabilities under the warranty shall be limited to
correcting or replacing defective workmanship or materials. A claimant under the
warranty must notify Custom BioGenic Systems within (10) days after the discovery
of the defect. Custom BioGenic Systems reserves the right, at their discretion, to
correct the defect(s) in the eld without return shipment to the factory.
This warranty does not cover defects on cryogenic equipment resulting from
abusive handling and subsequent structural failure.
To register you Custom BioGenic Systems product(s) please go to
www.custombiogenics.com/registration.html
Controller Serial Number: Model:
Freezer Serial Number: Model:
For Technical Assistance Call: 1.800.523.0072 (U.S. Only)
Phone: [phone] Fax: [phone]
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IMPORTANT INFORMATION

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TABLE OF CONTENTS
Q&A Page 1
Safety 2-3
V Series and S Series Vessel Descriptions 4
Initial Set-Up 5
Operation 5
Front Panel Controls (ALL) 6
2301 Back Panel 7
Series 5000 Back Panel 8
V Series Manual Fill Instructions 9
Feature Details 10-11
Remote Alarm Wiring 12
Storage Unit Parameters 12
LCD Display Functions 13
Standard Operating Mode 14
Programming Mode 16
Verication & Calibration
High & Low Alarm Set Point Verication 26
Temperature Display Verication 26
2301 Temperature Calibration 27
V Series Level Display Verication & Calibration 28
Trouble Shooting 29-33
Plumbing Wiring Diagram Part ID 34
Plumbing Assembly Diagram 35
2301 Controller Wiring Diagram 36
2301 Controller Diagram Part ID 37
5000 2301 Controller Diagram Part ID 38
Series 2301 Controller Back Panel Part ID 39
Series 5000 2301 Controller Back Panel Part ID 40
90 Series Tank Diagram Part ID 41
S-1500 Series Tank Diagram Part ID 42
V-1500 Tank Diagram Part ID 43
3000 Tank Diagram Part ID 44
5000 & 5000EH Tank Diagram Part ID 45
Console and Rear Service Cover Assembly 46
Probe Assembly Diagram Part ID 47
Cleaning and Maintenance 48
EMC Test Report 49
Notes 50

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Recommended Best Practices in the industry for safe sample storage
Secondary or backup alarm
It is strongly recommended to have, at a minimum, an Independent Temperature Alarm
for each LN2 freezer.
Remote alarm connection
It is strongly recommended to have, at a minimum, a Remote Auto Dialer connected to
your Delta Room, Facility Monitoring Station or Remote Auto Dialer for each LN2 freezer.
Split Samples
Separate samples in to two dierent freezers.
Keep a daily log
Track temperature levels daily.
Record ll intervals and amount of LN2 lled into vessel (manual or pour-ll freezer or
dewar).
This information should be documented daily and reviewed monthly to foresee and
prevent future problems such as temperature uctuations and varying liquid levels.
Prepare a contact list
Have at least 3 people on your contact list with Home, Cell and Pager Number. Review the
list regularly for accuracy and changes.
Contingency plan
What to do if no one returns a call from your call list.
What to do if we run out of LN2 and it is a weekend or holiday.
What to do if you have a High Temp Alarm.
What to do if you have a Low Level Alarm.
What to do if you have a High Level Alarm.
What to do if you lose power.
What to do if you get a Source Alarm.
RECOMMENDED BEST PRACTICES

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QUESTIONS and ANSWERS
Keep your most important samples safe.
You can plan for the future, you can’t change the past.
Back-up or secondary Alarm
Q: Why do I need a Secondary or Back-Up Alarm?
A: Your freezer could malfunction. By having a secondary or back-up alarm you can minimize
the possibility of a loss due to a malfunction.
Remote Alarm Connection to your Delta Room, Facility Monitoring Station or Remote Auto
Dialer
Q: Why should I use my remote alarm feature or connection?
A: If an alarm occurs after hours, on a weekend or holiday the remote alarm connection will alert
you to a problem and let you address it quickly.
Q: I am not sure how to connect the alarm or what to connect the alarm to.
A: First, contact your lab manager, supervisor or in house biotech representative. If no one is
sure what to do, contact CBS customer service for help and direction.
Q: What is an Auto Dialer and why would I use one?
A: An Auto Dialer is used when a central connection is not available for the remote alarm. When
connected to a telephone land line the Auto Dialer will dial up to 8 dierent contacts.
If you have any question, comments or concerns please contact:
Custom BioGenic Systems Customer Service
Phone [phone]
Toll free (US only) 800.523.0072
Email [email protected]

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WARNING
SAFETY
Go to custombiogenics.com/technicalguides.html
for more information.
IMPORTANT: The following section on SAFETY should be read carefully and followed completely. Warnings
regarding the handling of liquid nitrogen are by no means a complete volume on the safe use of cryogenic liq-
uids. All personnel should have complete knowledge of the correct procedures, as well as the hazards of work-
ing with liquid nitrogen. Failure to do so could result in serious injury or death. Be sure to read and understand
the Liquid Nitrogen Safety Data Sheet provided by your Liquid Nitrogen Supplier.
To avoid risk of electric shock, this equipment must only be connected to a supply mains with protective
earth.
The lid could present a crush hazard. Open the lid entirely and carefully to prevent the lid from
improperly engaging and falling. Use both hands while lowering lid into the closed position.
To prevent a rolling hazard, ensure the casters are locked.
HAZARDS ASSOCIATED WITH LIQUID NITROGEN:
Asphyxiation
When liquid is released into a conned space it displaces oxygen from the air. This results in an
oxygen-decient atmosphere, particularly if vented into a closed space, and asphyxia can result. Entering an
oxygen decient room can cause unconsciousness without warning. Always check air quality upon entering a
room where cryogenic liquids are being used, and if possible, have an air respirator available.
Cold Burns
Due to its extremely low temperature, the cold vapor and gas that are produced when liquid nitrogen boils
can give rise to cold burns or frostbite upon contact with unprotected skin. Exposure may also produce
discomfort in breathing and can provoke an asthma attack in susceptible individuals. Never allow direct skin
contact with liquid nitrogen or serious frostbite will result. Personal Protective Equipment (PPE) must be used,
based on the principle that it should prevent liquid nitrogen from touching the skin and eyes. Appropriate PPE
includes:
• Cryo-gloves (elbow length and loose tting).
• Safety goggles/full face shield.
• Long sleeve lab coat.
• Cryo-apron.
• Long pants that come over the top of shoes.
• Fully enclosed footwear.

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SAFETY
Boiling, Splashing and Spurting
Introducing equipment which is at room temperature into liquid nitrogen is always somewhat hazardous.
Beware of splashing and “boiling”which may occur. Never dip a hollow tube into liquid nitrogen; it may
spurt liquid nitrogen.
Compatibility
Liquid nitrogen should ony be handled and transported in approved containers. Liquid nitrogen may alter
the physical characteristics of many materials, make them brittle and fail. Liquid nitrogen should never be
used in combination with other substances or materials without knowing what the result will be. When in
doubt, contact a competent authority.
Fire in Oxygen-Enriched Atmostphere
Liquid Nitrogen can condense oxygen from the atmosphere. Liquid oxygen creates potential-
ly ammable or explosive conditions by greatly increasing the combustibility of many materials
(e.g. solvents, hydrocarbons).
FIRST AID
In the event a person has frostbite from liquid nitrogen, the following rst aid treatment should be given
while awaiting the arrival of medics or a doctor.
1. If the material has contaced skin or eyes, ood those areas with large quantities of unheated water
and protect frozen areas with loose, bulky, dry, and sterile dressings.
2. If the skin is blistered or there is a chance that the eyes have been aected, seek medical help
immediatley.
SPECIFIC FIRST AID PROCEDURES: PROMPT MEDICAL ATTENTION IS MANDATORY IN ALL CASES OF
OVEREXPOSURE TO NITROGEN. RESCUE PERSONNEL SHOULD BE EQUIPPED WITH SELFCONTAINED
BREATHING APPARATUS.
INHALATION: Conscious persons should be assisted to an uncontaminated area and inhale fresh air. Quick
removal from the contaminated area is most important. Unconscious persons should be moved to an
uncontaminated area, given mouth-to-mouth resuscitation and supplemental oxygen. Further treatment
should be symptomatic and supportive.
SKIN OR EYE CONTACT: Flush aected areas with lukewarm water. DO NOT USE HOT WATER. A physician
should see the patient promtly, especially if the cryogenic “burn” has resulted in blistering

Throughout this manual references are made to the “S Series”
and “V Series” storage unit vessels. In order to give a complete
understanding of the dierence between these two models,
the diagrams at right are being provided. They show clearly the
dierence between the two, and the characteristics of each.
Although the two are quite dierent physically, they operate in
essentially the same way, and the electronic controls are identical for
them both. Whenever there is a dierent procedure or operation for
one, we’ve clearly stated the dierent instructions for each model.
When not stated as dierent, the procedure or operation is the same
for both.
What makes these two designs so dierent is this basic fact:
With the S Series models, the liquid nitrogen is contained
within the storage vessel, and the stored samples are, or are
potentially, in contact with the liquid.
With the V Series or Isothermal models, the liquid nitrogen
is contained inside the vessel walls, and the stored samples never
come into contact with the liquid nitrogen except in the case of an
accidental overll.
To understand the way the system operates, see the
OPERATION section on the next page for a basic explanation of
the mechanics of the system. Also, be sure to read this manual
completely before attempting to use this equipment. If you are still
not sure how to use this equipment properly, contact a competent
authority, rather than risking your safety or the safety of the stored
samples.
When the unit is placed into service, check daily to ensure
proper operation and safety of the stored samples. For the V Series
units, it is essential to lift the lid each day and check for vapor and
signs of proper freezing.
V SERIES (ISOTHERMAL) VESSEL
LIQUID NITROGEN IS CONTAINED INSIDE
THE VESSEL WALLS, AND DOES NOT
CONTACT THE INVENTORY SYSTEM.
S SERIES (STANDARD) VESSEL
LIQUID NITROGEN IS CONTAINED WITHIN
THE VESSEL, AND IS DIRECT CONTACT WITH
THE INVENTORY SYSTEM.
V SERIES AND S SERIES DESCRIPTIONS
Go to custombiogenics.com/technicalguides.html
for more information.
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