cytiva Monitor pH/C-900 User manual
Page intentionally left blank
Table of Contents
1 Introduction ........................................................................................................ 5
1.1 About this manual ......................................................................................................................................... 6
1.2 Important user information ....................................................................................................................... 7
2 Safety instructions ............................................................................................ 9
2.1 Safety precautions ........................................................................................................................................ 10
2.2 Labels ................................................................................................................................................................. 13
2.3 Emergency procedures ............................................................................................................................... 14
3 Instrument description ...................................................................................... 15
4 Installation .......................................................................................................... 19
4.1 Site requirements .......................................................................................................................................... 20
4.2 Unpacking ......................................................................................................................................................... 22
4.3 Installing the conductivity cell .................................................................................................................. 23
4.4 Installing the pH electrode ......................................................................................................................... 24
4.5 Connecting to chart recorder ................................................................................................................... 27
4.6 Connecting to communication link ........................................................................................................ 28
4.7 Connecting to supply voltage ................................................................................................................... 29
4.8 Preparing the instrument for use ............................................................................................................ 30
5 Operation ............................................................................................................ 31
5.1 Starting the instrument ............................................................................................................................... 32
5.2 Menu selection and settings ..................................................................................................................... 33
5.3 Setup menu ...................................................................................................................................................... 40
5.4 Reading pH and conductivity values ...................................................................................................... 50
5.5 Setting conductivity scale .......................................................................................................................... 51
5.6 Calibrating pH and conductivity .............................................................................................................. 52
5.7 Using an external chart recorder ............................................................................................................. 55
5.8 Storage and shut-down ............................................................................................................................... 56
6 Maintenance ....................................................................................................... 57
6.1 Cleaning before planned service ............................................................................................................. 58
6.2 User maintenance schedule ...................................................................................................................... 59
6.3 Cleaning ............................................................................................................................................................ 60
6.4 Component maintenance .......................................................................................................................... 63
7 Troubleshooting ................................................................................................. 64
7.1 Problems and corrective actions ............................................................................................................. 65
7.2 Error messages ............................................................................................................................................... 69
8 Reference information ...................................................................................... 71
8.1 Menu overview ................................................................................................................................................ 72
8.2 Technical specifications .............................................................................................................................. 73
8.3 Accessories and spare parts ...................................................................................................................... 76
8.4 Recycling information .................................................................................................................................. 78
Table of Contents
Monitor pH/C-900 Operating Instructions 29054925 AF 3
8.5 Regulatory information ............................................................................................................................... 79
8.5.1 Contact information .................................................................................................................................... 80
8.5.2 European Union and European Economic Area .............................................................................. 81
8.5.3 Eurasian Economic Union
Евразийский
экономический
союз
.................................................................................................. 82
8.5.4 Regulations for North America ............................................................................................................... 84
8.5.5 Regulatory statements .............................................................................................................................. 85
8.5.6 Declaration of Hazardous Substances (DoHS) ................................................................................ 86
8.6 Health and Safety Declaration Form ...................................................................................................... 88
Table of Contents
4 Monitor pH/C-900 Operating Instructions 29054925 AF
1 Introduction
About this chapter
This chapter contains important user information, descriptions of safety notices, regu-
latory information, and intended use of the Monitor pH/C-900 instrument.
In this chapter
Section See page
1.1 About this manual 6
1.2 Important user information 7
1 Introduction
Monitor pH/C-900 Operating Instructions 29054925 AF 5
1.1 About this manual
Purpose of this manual
The Operating Instructions provide you with the information needed to install, operate
and maintain the product in a safe way.
Typographical conventions
Software items are identified in the text by bold italic text.
Hardware items are identified in the text by bold text.
In electronic format, references in italics are clickable hyperlinks.
1 Introduction
1.1 About this manual
6 Monitor pH/C-900 Operating Instructions 29054925 AF
1.2 Important user information
Read this before operating the
product
All users must read the entire Operating Instructions before installing, oper-
ating or maintaining the product.
Always keep the Operating Instructions at hand when operating the product.
Do not operate the product in any other way than described in the user documenta-
tion. If you do, you may be exposed to hazards that can lead to personal injury and you
may cause damage to the equipment.
Intended use of the product
Monitor pH/C-900 is intended for research use only, and shall not be used in any clinical
procedures, or for diagnostic purposes.
Prerequisites
In order to operate Monitor pH/C-900 safely, and according to the intended purpose,
the following prerequisites must be met:
•You should be acquainted with the use of bioprocessing equipment and with the
handling of biological materials.
•You must read and understand the Safety chapter of these Operating Instructions.
•The system must be installed according to the instructions in the Installation
chapter.
Definitions
This user documentation contains safety notices (WARNING, CAUTION, and NOTICE)
concerning the safe use of the product. See definitions below.
WARNING
WARNING indicates a hazardous situation which, if not avoided,
could result in death or serious injury. It is important not to proceed
until all stated conditions are met and clearly understood.
1 Introduction
1.2 Important user information
Monitor pH/C-900 Operating Instructions 29054925 AF 7
CAUTION
CAUTION indicates a hazardous situation which, if not avoided,
could result in minor or moderate injury. It is important not to
proceed until all stated conditions are met and clearly understood.
NOTICE
NOTICE indicates instructions that must be followed to avoid
damage to the product or other equipment.
Notes and tips
Note: A note is used to indicate information that is important for trouble-free and
optimal use of the product.
Tip: A tip contains useful information that can improve or optimize your proce-
dures.
1 Introduction
1.2 Important user information
8 Monitor pH/C-900 Operating Instructions 29054925 AF
2 Safety instructions
About this chapter
This chapter describes safety compliance, safety labels, general safety precautions,
emergency procedures, power failure and recycling of Monitor pH/C-900.
Important
WARNING
Before installing, operating or maintaining the product, all
users must read and understand the entire contents of this
chapter to become aware of the hazards involved.
In this chapter
Section See page
2.1 Safety precautions 10
2.2 Labels 13
2.3 Emergency procedures 14
2 Safety instructions
Monitor pH/C-900 Operating Instructions 29054925 AF 9
2.1 Safety precautions
Introduction
The safety precautions in this section are grouped into the following categories:
•General precautions
•Personal protection
•Installing and moving the system
•System operation
•Maintenance
General precautions
Always follow these General precautions to avoid injury when using the Monitor pH/
C-900 instrument.
WARNING
Do not operate the product in any other way than described in the
user documentation.
WARNING
Only properly trained personnel may operate and maintain the
product.
NOTICE
Any computer used with the equipment must comply with
IEC 60950 or IEC 62368-1 and be installed and used according to
the manufacturer's instructions.
Personal protection
WARNING
Always use appropriate Personal Protective Equipment (PPE)
during operation and maintenance of this product.
2 Safety instructions
2.1 Safety precautions
10 Monitor pH/C-900 Operating Instructions 29054925 AF
WARNING
Hazardous substances and biological agents. When using
hazardous chemical and biological agents, take all suitable protec-
tive measures, such as wearing protective clothing, glasses and
gloves resistant to the substances used. Follow local and/or
national regulations for safe operation and maintenance of this
product.
WARNING
Spread of biological agents. The operator must take all neces-
sary actions to avoid spreading hazardous biological agents. The
facility must comply with the national code of practice for
biosafety.
Installing and moving the instrument
WARNING
Protective ground. The product must always be connected to a
grounded power outlet.
WARNING
Power cord. Only use power cords with approved plugs delivered
or approved by Cytiva.
WARNING
Access to power switch and power cord with plug. Do not
block access to the power switch and power cord. The power switch
must always be easy to access. The power cord with plug must
always be easy to disconnect.
CAUTION
Make sure that the system is placed on a stable, level bench with
adequate space for ventilation.
2 Safety instructions
2.1 Safety precautions
Monitor pH/C-900 Operating Instructions 29054925 AF 11
System operation
WARNING
If liquid is spilled on the equipment, the electrical power supply
must be disconnected immediately. The equipment must be
completely dry on the inside and the outside before reconnecting
the power supply.
Maintenance
WARNING
Electrical shock hazard. All repairs should be done by service
personnel authorized by Cytiva. Do not open any covers or replace
parts unless specifically stated in the user documentation.
WARNING
Disconnect power. Always disconnect power from the instru-
ment before performing any maintenance task.
WARNING
Use only approved parts. Only spare parts and accessories that
are approved or supplied by Cytiva may be used for maintaining or
servicing the product.
WARNING
Corrosive substance. NaOH is corrosive and therefore dangerous
to health. When using hazardous chemicals, avoid spillage and
wear protective glasses and other suitable Personal Protective
Equipment (PPE).
CAUTION
Hazardous chemicals or biological agents in UV flow cell.
Make sure that the entire flow cell has been flushed thoroughly
with bacteriostatic solution (e.g., NaOH) and distilled water, before
service and maintenance.
2 Safety instructions
2.1 Safety precautions
12 Monitor pH/C-900 Operating Instructions 29054925 AF
2.2 Labels
Introduction
This section describes the system label and other safety or regulatory labels that are
attached to the product.
System label
The system label is located on the back of the equipment. The system label identifies
the equipment and shows electrical data, regulatory compliance, and warning
symbols.
Description of symbols on the system
label
The following symbols and text may be present on the system label:
Symbol/text Meaning
Code no Product code
Serial no Serial number
Mfg Year Manufacturing year (YYYY) and month (MM)
Voltage
Frequency
Max Power
Electrical requirements:
•Supply voltage (VAC )
•Supply voltage frequency (Hz)
•Max. power consumption (VA)
Warning! Read the user documentation before using the
system. Do not open any covers or replace parts unless
specifically stated in the user documentation.
2 Safety instructions
2.2 Labels
Monitor pH/C-900 Operating Instructions 29054925 AF 13
2.3 Emergency procedures
Introduction
This section describes how to shut down the Monitor pH/C-900 instrument in an emer-
gency situation.
The section also describes the result in the event of power failure.
Emergency shutdown
In an emergency situation, follow the steps below to stop the run:
Step Action
1Press the On/off switch located on the rear panel to the (O) position to turn
off the power to the instrument.
2If required, disconnect the mains power cord.
Result:
The run is interrupted immediately.
Restart after power failure
If the power supply to the instrument is interrupted, the instrument automatically
restarts itself and displays the main operating window. All set values are retained in the
instrument.
2 Safety instructions
2.3 Emergency procedures
14 Monitor pH/C-900 Operating Instructions 29054925 AF
3 Instrument description
About this chapter
This chapter gives an overview of the Monitor pH/C-900 instrument, and a brief
description of its function.
Introduction to Monitor pH/C-900
Monitor pH/C-900 is an on-line monitor for measurement of pH and conductivity. The
monitor can work with standard glass pH electrodes with a built in liquid-filled refer-
ence electrode and BNC connector.
The conductivity monitor has a very large dynamic range from 1 μS to 999.9 mS/cm and
is therefore suitable for a wide range of applications.
Main unit
The illustration below shows the Monitor pH/C-900 instrument.
12 3
Part Function
1 Main unit
2 pH electrode
3 Conductivity flow cell
3 Instrument description
Monitor pH/C-900 Operating Instructions 29054925 AF 15
Main unit, rear panel
The illustration below shows the rear panel of the Monitor pH/C-900 instrument.
1 2 3 4 5 6 7 8
pH
Ground
pH
Probe
UniNet -1
Mains On/o
Analogue out 0-1V
pH Cond
Conductivity
Flow Cell
Part Function
1Conductivity Flow Cell. Connection to conductivity flow cell, 9 pole D-
sub connector
2pH Ground. pH signal ground
3pH Probe. Connection to pH electrode, standard BNC socket
4Analog out 0–1V pH. Separate recorder output for pH, recorder output
0–1 V
5Analog out 0–1V Cond. Separate recorder output for conductivity,
recorder output 0–1 V
6UniNet-1. Computer network
7Mains. Supply voltage, grounded
8On/off. Instrument power switch
3 Instrument description
16 Monitor pH/C-900 Operating Instructions 29054925 AF
pH electrode
The pH electrode is of the sealed combination double junction type. It contains a sealed
Ag/AgCl reference which cannot be refilled, an internal electrolyte bridge of 4M KCl
saturated with Ag/AgCl, an outer electrolyte bridge of 1M KNO3, an annular ceramic
reference junction and a low profile pH membrane. The pH electrode is delivered with a
transparent cover.
1
2
3
5
6
7
8
10
9
4
Part Function
1 Sealing
2 Internal electrolyte bridge containing 4M KCl saturated with AgCl
3 Silicone sealing
4 Ag/AgCl reference electrode
5 Internal annular coaxial ceramic reference junction
6 External electrolyte bridge containing 1M KNO3
7 O-ring
8 Glass electrode containing diluted, buffered KCl
3 Instrument description
Monitor pH/C-900 Operating Instructions 29054925 AF 17
Part Function
9 O-ring
10 External annular coaxial ceramic reference junction
Conductivity cell
The flow cell has two cylindrical titanium electrodes positioned in the flow path of the
cell. An alternating voltage is applied between the electrodes and the resulting current
is measured and used to calculate the conductivity of the eluent. The monitor controls
the AC frequency and increases it with increasing conductivity between 50 Hz and
50 kHz giving maximum linearity and true conductivity values.
The conductivity is automatically calculated by multiplying the measured conductance
by the flow cell’s cell constant. The cell constant is pre-calibrated on delivery but can be
measured with a separate calibration procedure, see Calibrating the conductivity cell,
on page 41.
1
2
One of the electrodes has a small temperature sensor (1) for measuring the tempera-
ture of the eluent (2) in the flow cell. Temperature variations influence the conductivity
and in some applications, when highly precise conductivity values are required, it is
possible to program a temperature compensation factor that recalculates the conduc-
tivity to a set reference temperature.
3 Instrument description
18 Monitor pH/C-900 Operating Instructions 29054925 AF
4 Installation
About this chapter
This chapter provides required information to enable users and service personnel to
unpack, install, move and transport Monitor pH/C-900.
Safety precautions
WARNING
Before attempting to perform any of the procedures described in
this chapter, you must read and understand all contents of the
Safety instructions chapter.
In this chapter
Section See page
4.1 Site requirements 20
4.2 Unpacking 22
4.3 Installing the conductivity cell 23
4.4 Installing the pH electrode 24
4.5 Connecting to chart recorder 27
4.6 Connecting to communication link 28
4.7 Connecting to supply voltage 29
4.8 Preparing the instrument for use 30
4 Installation
Monitor pH/C-900 Operating Instructions 29054925 AF 19
4.1 Site requirements
Space requirements
Prepare a clean working area on a stable laboratory bench. The bench must comply
with the specifications in the following table.
Parameter Specification
Minimum bench area for operating
Monitor pH/C-900
70 x 60 cm
Free space required around the imager At least 40 cm free space in front of the
instrument
10 cm free space on all other sides
Space for touchscreen or monitor with
keyboard and mouse
60 x 60 cm
Load capacity 980 N (100 kg) or higher
Inclination of bench surface Horizontal ±2°
WARNING
Access to power cord. Do not block access to the power cord. The
power cord must always be easy to disconnect.
Environmental requirements
The following general requirements must be fulfilled:
•The room must have exhaust ventilation
•The instrument should not be exposed to sources of heat, such as direct sunlight
•Dust in the atmosphere should be kept to a minimum
•The equipment must not be exposed to vibrations
The installation site must comply with the following specifications.
Parameter Requirement
Allowed location Indoor use only
Ambient temperature, operation 5°C to 40°C
Ambient temperature, storage -25°C to 70°C
4 Installation
4.1 Site requirements
20 Monitor pH/C-900 Operating Instructions 29054925 AF
Table of contents
