Dionex ASE 100 User manual

ASE 100®
ACCELERATED SOLVENT EXTRACTOR
OPERATOR'S MANUAL
© 2002 Dionex Corporation
Document No. 031842
Revision 01
May 2002

©2002 by Dionex Corporation
All rights reserved worldwide.
Printed in the United States of America.
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electronic, mechanical, magnetic, manual, or otherwise, or disclosed to third parties
without the express written permission of Dionex Corporation, 1228 Titan Way,
Sunnyvale, California 94088-3603 U.S.A.
DISCLAIMER OF WARRANTY AND LIMITED WARRANTY
THIS PUBLICATION IS PROVIDED “AS IS” WITHOUT WARRANTY OF
ANY KIND. DIONEX CORPORATION DOES NOT WARRANT,
GUARANTEE, OR MAKE ANY EXPRESS OR IMPLIED
REPRESENTATIONS REGARDING THE USE, OR THE RESULTS OF THE
USE, OF THIS PUBLICATION IN TERMS OF CORRECTNESS, ACCURACY,
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CORPORATION RESERVES THE RIGHT TO REVISE THIS PUBLICATION
AND TO MAKE CHANGES FROM TIME TO TIME IN THE CONTENT
HEREINOF WITHOUT OBLIGATION OF DIONEX CORPORATION TO
NOTIFY ANY PERSON OR ORGANIZATION OF SUCH REVISION OR
CHANGES.
TRADEMARKS
AutoSeal™ is a trademark and ASE®100 is a registered trademark of Dionex
Corporation.
Teflon®and Viton®are registered trademarks of E. I. duPont de Nemours &
Company.
PRINTING HISTORY
Revision 01, May 2002

Doc. 031842-01 5/02 i
Contents
1 • Introduction
1.1 Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1
1.2 About This Manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-3
1.2.1 Typefaces . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-4
1.2.2 Safety Messages and Notes . . . . . . . . . . . . . . . . . . . . . . . . . . .1-4
1.3 Safety Labels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-6
2 • Description
2.1 Operating Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1
2.1.1 Control Panel Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-3
2.1.2 Control Panel Keypad . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-4
2.1.3 Extraction Cells and Rinse Cells . . . . . . . . . . . . . . . . . . . . . . .2-6
2.1.4 Collection Bottle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-7
2.1.5 Solvent Bottle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-8
2.1.6 Waste Bottle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-9
2.2 Rear Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-9
2.3 Extraction Process . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-11
2.4 Method Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-13
2.5 Preprogrammed Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-14

ASE 100 Accelerated Solvent Extractor
ii Doc. 031842-01 5/02
3 • Operation and Maintenance
3.1 Preparing to Run an Extraction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-1
3.1.1 Selecting and Preparing Solvent . . . . . . . . . . . . . . . . . . . . . . . 3-1
3.1.2 Filling the Solvent Bottle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3
3.1.3 Preparing the Sample . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-5
3.1.4 Installing the Cell Filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-7
3.1.5 Filling the Cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-9
3.1.6 Installing the Collection Bottle . . . . . . . . . . . . . . . . . . . . . . . 3-11
3.1.7 Rinsing/Priming the System . . . . . . . . . . . . . . . . . . . . . . . . . 3-12
3.2 Editing a Custom Method (1–24) . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-13
3.3 Guidelines for Method Development . . . . . . . . . . . . . . . . . . . . . . . . . 3-17
3.4 Running an Extraction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-20
3.5 Post-Extraction Procedures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-24
3.5.1 Cleaning the Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-24
3.5.2 Processing Extracts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-24
3.6 Aborting a Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-25
3.7 Routine Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-26
3.7.1 Daily Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-26
3.7.2 Periodic Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-26
3.8 Shutdown . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-27

Contents
Doc. 031842-01 5/02 iii
4 • Troubleshooting
4.1 Error Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1
4.2 Liquid Leaks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-8
4.3 Gas Leaks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-10
4.4 System Stops . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-10
5•Service
5.1 Replacing the Seal in the Cell End Cap . . . . . . . . . . . . . . . . . . . . . . . . .5-2
5.2 Replacing the Cell O-Ring . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-4
5.3 Removing the Right Side Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-5
5.4 Cleaning and/or Replacing Pump Check Valves . . . . . . . . . . . . . . . . . .5-8
5.4.1 Removing the Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-8
5.4.2 Removing the Check Valves . . . . . . . . . . . . . . . . . . . . . . . . .5-10
5.4.3 Cleaning the Check Valves . . . . . . . . . . . . . . . . . . . . . . . . . .5-10
5.4.4 Reinstalling the Inlet Check Valve . . . . . . . . . . . . . . . . . . . . .5-10
5.4.5 Reinstalling the Outlet Check Valve . . . . . . . . . . . . . . . . . . .5-11
5.4.6 Reinstalling the Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-11
5.4.7 Completing the Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . .5-11
5.5 Replacing the Piston Seal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-12
5.5.1 Removing the Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-12
5.5.2 Replacing the Piston Seal . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-13
5.5.3 Reinstalling the Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-14
5.5.4 Completing the Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . .5-14
5.6 Replacing the Relief Valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-15