DORIC Bundle-imaging Fiber Photometry System Gen3 User manual

Bundle-imaging Fiber Photometry System
Gen3
User Manual
Version 1.0.0

Contents
1 Introduction 3
2 System Overview 4
3 Getting Started : General Setup Guidelines 6
4 Specifications 7
4.1 General specifications ............................................... 7
4.2 Optical specifications ................................................ 8
5 Support 9
5.1 Maintenance ..................................................... 9
5.2 Warranty ....................................................... 9
5.3 Contact us ...................................................... 9
2

1
Introduction
Doric’s Bundle-imaging Fiber Photometry System Generation 3 (BFMC Gen3) is a fully integrated, offering a hassle-free,
plug-and-play solution, that represents an elegant alternative for multiple site measurements. To record green and
red photometry signals, the BFMC uses built-in LEDs to illuminate the entire sample port, and a CMOS sensor to
simultaneously image the fluorescent signal from every fiber within the bundle. The fluorescent light collected from
each fiber within the bundle creates circular spots on a CMOS sensor. The electrical read-out from pixels within
each fiber image correlates with the calcium activity of the corresponding brain site within Doric Neuroscience Studio
(Fig.1.1b). The system is available for single and dual color measurements with isosbestic reference excitation (Fig.1.1a).
(a) BFMC system (Fan Out configuration) (b) Doric Neuroscience Studio BFPS interface
Figure 1.1: Bundle-Imaging Fiber Photometry System (BFPS) overview
3

2
System Overview
•The BFMC system is also equipped with 8 Digital Input/Output (DIO) ports and an DB25 adapter (see pinout
description on Fig. 2.2). These ports are usefull for synchronization and closed-loop experiments with external
devices such as behavior cameras, operant conditioning chambers, video tracking software, and optogenetic
light sources (Fig. 2.1).
•Each BFMC Gen3 has a single sample port (Fig. 2.1). This port doesn’t have spectral filtering, all wavelengths
can pass freely through it. The sample port consists of a microscope lens and a fiber adapter to image fiber
bundle onto the cameras. To accommodate larger fiber bundles (up to 2.5 mm of diameter), an SMA receptacle
is used on the sample port. Two types of bundle can be connected to the SMA port: A Fan Out bundle where
each fiber has an individual connector at its other end, or a High Density fiber that has multiple fiber-optic
strands terminated on one side in the same high-density connector pattern as the corresponding High-Density
Fiber-optic Cannula Array. Low autofluorescence materials and black epoxy are used to reduce background
fluorescence and prevent cross-talk between each fiber.
•The BFMC Gen3 is also equipped with a power switch and 2 status lights turning ON when the device is powered
on and connected to Doric Neuroscience Studio software (Fig. 2.1).
•The 12 VDC power input port is used to power the BFMC Gen3 (Fig. 2.1).
•The USB-3 port is used to connect the BFMC Gen3 to the computer via a USB-3 cable.
Figure 2.1: BFMC Gen3 overview
4

Figure 2.2: DB25 port pinout description
Chapter 2. System Overview 5

3
Getting Started : General Setup Guidelines
A USB-3 cable and power cables are included with the Bundle-imaging Fiber Photometry System. Figure 3.1 illustrates
connection of the system to a computer.
Figure 3.1: Bundle-imaging Fiber Photometry System : Connection to a computer.
1. Connect a USB 3.0 cable between the BFMC Gen3 system and a USB-3 port of the computer that will be used
for experimentation.
2. Connect a Bundle Branching Fiber Optic Patchcord to the Sample Port.
3. Connect the BFMC Gen3 to the 12 V AC/DC and 36W power supply.
4. Open Doric Neuroscience Studio. To set up an experiment, refer to Doric Neuroscience Studio user manual.
6

4
Specifications
4.1 General specifications
Table 4.1: General specifications for BFMC Gen3
SPECIFICATION VALUE UNIT
Bundle-imaging Fluorescence Mini Cube
Wavelength range 350 to 1100 nm
Field of view 2.5 mm
Objective NA 0.4 -
Optical Fiber connector SMA -
Max. number of sites - 20x core 400 µm NA0.37 -
- 60x core 200 µm NA0.37
- 100x core 100 µm NA0.37
Excitation Uniformity 10% over FOV -
Optical fiber compatibility Core diameter 100, 200, or 400 µm NA 0.37 -
Optical fiber attenuation OD 5 outside band -
Built-in LEDs
Max Current 500 mA
Maximum Output Power See Table 4.3 -
Sensor
CMOS image sensor Sony IMX174LLJ -
Pixel Size 5.86 x 5.86 µm
Resolution 1024 x 1024 pixels
Quantum Efficiency 82%at 520nm
Frame Rate up to 60 Hz
Power consumption (supplied by USB) 200 mA
DI/O
Number of ports 32 -
Maximum sampling rate 10 kSps
Ports description 8 BNC I/O + 1 DB25 (8 Input/Output, 8 Input,
8 Output)
-
7

Physical properties
Size 310x310x66 mm
Mass 3700 g
Power supply
Voltage 110 - 240 VAC
DC power supply 12 VDC
Power 36 W
Output current 3 A
Table 4.2: Computer requirements for BFMC Gen3 installation
Operating system Microsoft 10, 64 bit
Memory 8 GB RAM minimum (16 GB recommended)
Processor speed 3 GHz and 8 cores
Hard drive 500 MB of free hard disk space (SSD recommended)
Data link USB3.0 (cable included)
4.2 Optical specifications
Table 4.3: Typical Built-in LED Output Power vs Optical Fiber Core Diameter
LED TYPICAL OUTPUT POWER
@200 mA,CW (mW)
Central
Wavelength (nm)
Bandwidth
FWHM (nm)
Core 200 µm
0.37 NA
Core 400 µm
0.57 NA
405 10 ∼0.100 ∼0.700
415 10 ∼0.130 ∼0.500
474 23 ∼0.180 ∼0.700
563 9 ∼0.020 ∼0.130
Table 4.4: Typical filter configuration of BFMC Gen3
Fluorescence Mini Cubes Excitation (nm) Fluorescence (nm)
GCaMP
Excitation 1 (isosbestic) 400-410 or 410-420
Emission 2 (functional) 460-490 500-550
GCaMP + red fluorophore
Excitation 1 (isosbestic) 400-410 or 410-420
Excitation 2 (functional) 460-490 500-550
Excitation 3 (red fluorophore) 555-570 580-680
Chapter 4. Specifications 8

5
Support
5.1 Maintenance
The product does not require any specific maintenance. Contact Doric Lenses for return instructions if the unit does
not work properly and needs to be repaired.
5.2 Warranty
This product is under warranty for a period of 12 months. Contact Doric Lenses for return instructions. This warranty
will not be applicable if the unit is damaged or needs to be repaired as a result of improper use or operation outside
the conditions stated in this manual. For more information, see our Website.
5.3 Contact us
For any questions or comments, do not hesitate to contact us by:
Phone [phone]
Email [email protected]
©2023 DORIC LENSES INC
357 rue Franquet - Quebec, (Quebec)
G1P 4N7, Canada
Phone: [phone] - Fax: [phone]
www.doriclenses.com
9