Hanna Instruments HI 3857 User manual

Instruction Manual
www.hannainst.com
DearCustomer,
Thank you for choosing a Hanna Product.
Please read the instructions carefully before using the
chemical test kit. It will provide you with the necessary
information for correct use of the kit.
Remove the chemical test kit from the packing material and
examine it carefully to make sure that no damage has
occurred during shipping. If there is any noticeable dam-
age, notify your Dealer or the nearest Hanna office
immediately.
Each kit is supplied with:
•HI 3857A-0 Detergents Reagent, 1 bottle with drop-
per (15 mL);
•HI 3857B-0 Detergents Reagent, 1 bottle with drop-
per (15 mL);
•Chloroform, 1 glass bottle (180 mL);
• Demineralizer Bottle, 1 bottle with filter cap for
about 12 liters of deionized water (depending on the
hardness level of water to be treated).;
• 1checkerdisc;
• 2 long glass vials (30 mL) with caps;
•1 long plastic pipette;
•1 plastic pipette (3 mL);
•1 plastic pipette (1 mL).
Note: Any damaged or defective item must be returned in
its original packing materials.
HI 3857
Detergents
Test Kit
ISTR3857 11/99 PRINTED IN ITALY
Detergents can enter water and wastewater by discharge of
domestic and industrial cleansing waters. The most widely
used detergents are linear alkyl sulfonates (LAS) and alkyl
benzene sulfonates (ABS): LAS are preferable to ABS be-
cause they are biodegradable, thus readily decomposed by
microorganisms.ThepresenceofanionicLAS/ABS detergents
in natural waters should be below 0.1 mg/L and in raw
domestic wastewater in the range from 1 to 20 mg/L.
Note: mg/L is equivalent to ppm (parts per million).
Range 0.00 to 1.30 mg/L as ABS/LAS
Smallest Increment 0.02 mg/L
Analysis Method Colorimetric
Sample Size 20 mL
Number of Tests 35
Case Dimensions 235x175x115 mm (9.2x6.9x4.5")
150x150x220 mm (5.9x5.9x8.7")
Shipping Weight 1245 g (43.9 oz.)
SPECIFICATIONSSPECIFICATIONS
SPECIFICATIONSSPECIFICATIONS
SPECIFICATIONS
SIGNIFICANCE AND USESIGNIFICANCE AND USE
SIGNIFICANCE AND USESIGNIFICANCE AND USE
SIGNIFICANCE AND USE
CHEMICAL REACTIONCHEMICAL REACTION
CHEMICAL REACTIONCHEMICAL REACTION
CHEMICAL REACTION
READ THE ENTIRE INSTRUCTIONS BEFORE USING THE KIT
Note: Non soap anionic surfactants commonly used in
detergentformulations arestronglyresponsive.Soaps,
as alkali salts of fatty acids do not respond to the
test.
1-Remove the cap and
fill the Demineralizer
Bottle with tap water.
2-Replace the cap and
shake gently for at least
2 minutes. The deminer-
alizedwater isnowready.
Note: The ion exchange resin contained in the Demineral-
izer Bottle is provided with an indicator substance.
The indicator will change from green to blue when
the resin has been exhausted and has to be re-
placed with a new one.
3- Fill one glass vial with 5 mL of sample.
This is the blank. Insert the blank into
the left hand opening of the checker disc.
4- Fill the other glass vial with 20 mL of
the sample, up to the mark.
5-Add 2 drops of HI
3857A-0 Detergents
Reagent. Replace the
cap and swirl to mix.
INSTRUCTIONSINSTRUCTIONS
INSTRUCTIONSINSTRUCTIONS
INSTRUCTIONS
The aqueous solution containing the detergents is treated
with the colored indicator. The reaction product can be
extracted in the chloroform layer, whilst the original dyestuff
isinsolublein the organic medium. Theintensityofthecolor
developed is proportional to the concentration of the deter-
gents present.
6- Add 3 drops of
HI3857B-0De-
tergents
Reagent, re-
place the cap
andswirltomix.
Thesolution will
turn a light blue color.
7- Using the 3 mL plastic pipette,
add exactly 5 mL of Chloroform.
The chloroform will sink to the
bottom of the vial because it is
heavier than water.
8- Cap the vial tight and mix by inverting it slowly for 30
seconds. Let it stand for about 1 minute until the
chloroform layer separates from the aqueous layer. The
color of the aqueous layer will fade slightly, while the
chloroform layer turns slightly blue.
9- Using the long plastic pipette, remove the upper
aqueous layer and dispose of it. Do not remove
the lower chloroform layer too!
10-Open the upper part of the Dem-
ineralizer Bottle cap and, squirting
gently, add to the vial 20 mL of
demineralized water, up to the 25
mL mark.
11-Add 2 drops of HI 3857A-0
reagent.
12- Cap the vial tight and mix by inverting slowly for 30
seconds. Let it stand for at least 1 minute - until the
aqueous layer is unclouded- to allow the chloroform
5 mL
20 mL
5 mL

HEALTH AND SAFETYHEALTH AND SAFETY
HEALTH AND SAFETYHEALTH AND SAFETY
HEALTH AND SAFETY
REFERENCESREFERENCES
REFERENCESREFERENCES
REFERENCES
Standard Methods for the Examination of Water and Waste-
water, 18th edition, 1992
layer to separate from the aqueous layer, as described
above. Then, using the long pipette, remove the upper
aqueous layer and dispose of it.
13-Repeat from step 10 to 12. The colored chloroform layer
is the reacted sample.
14-Insert the reacted sample into the right hand opening
of the checker disc.
15-Hold the checker disc
so that a light source
illuminatesthesamples
from the back win-
dows.
16-Rotate the disc while looking at the color test windows
and stop when you find the color match. Read the
value in the result window and record it in mg/L (or
ppm) of Detergents (LAS and/or ABS).
17-If the color is too
intense to make a
color match, then
theoriginalsample
needs to be di-
luted.Inthiscase,
using the 1 mL
The chemicals contained in this kit may be hazardous if
improperly handled. Read Health and Safety Data Sheet
before performing this test.
1 mL
of sample
plastic pipette, add to the vial 1 mL of sample. Fill the
vial with demineralized water up to the 20 mL mark
exactly. Follow the procedure from step 5 to step 16 and
multiply the result obtained by 20. The blank is pre-
pared in the same way as described in point 3.
18-Before starting a new test rinse the glass vials and the
1 mL plastic pipette several times with sample to be
analyzed. Rinse the glass vials and the 1 mL plastic
pipette in tap water before storing.
Note: Do not mix up the plastic pipettes!
Always use the 3 mL pipette for adding the Chloro-
form, the long pipette for removing the aqueous layer
and the 1 mL pipette for adding the sample.
For best results: Intensely colored samples will make the
colormatching determination difficultandtheyshould
be adequately treated before performing the test.
Suspended matter in large amounts should be
removed by prior filtration.
The temperature should be higher than 15°C, other-
wise the sample may become turbid.
Caution: Ultraviolet radiation may cause fading of colors.
When not in use, keep the disc protected from light,
in a cool and dry place.